Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,924,204	C→T	R477H (CGT→CAT)	mnmG ←	5‑carboxymethylaminomethyluridine‑tRNA synthase subunit MnmG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,924,204	0	C	T	100.0%	81.4 / NA	24	R477H (CGT→CAT)	mnmG	5‑carboxymethylaminomethyluridine‑tRNA synthase subunit MnmG
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (9/15);  total (9/15)

GGCGCAGTCAGGTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTCAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTCGTCATCCACCAGGCCCAGTTCACGAC  >  NC_000913/3924129‑3924279                                                                            |                                                                            ggcgCAGTCAGGTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTCAGACGCTGatgctca                                                                        >  1:767884/1‑81 (MQ=255) ggcgCAGTCAGGTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTCAGACGCTGatgctca                                                                        >  1:767885/1‑81 (MQ=255)   cgcAGTCAGGTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTCAGACGCTGatgctcacg                                                                      <  2:767834/81‑1 (MQ=255)         cAGGTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTCAGACGCTGATGCTCACGCTCGa                                                                 >  2:767894/1‑80 (MQ=255)         cAGGTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTCAGACGCTGATGCTCACGCTCGa                                                                 >  1:767879/1‑80 (MQ=255)           ggTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTCAGACGCTGATGCTCACGCTCGatat                                                              <  2:767835/81‑1 (MQ=255)               aGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTCAGACGCTGATGCTCACGCTCGATATtctc                                                          <  2:767837/81‑1 (MQ=255)               aGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTCAGACGCTGATGCTCACGCTCGATATtctc                                                          <  2:767836/81‑1 (MQ=255)                   ttCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTCAGACGCTGATGCTCACGCTCGATATTCTCAAGt                                                      <  1:767838/81‑1 (MQ=255)                     cACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTCAGACGCTGATGCTCACGCTCGATATTCTCAAGtt                                                     <  2:767839/80‑1 (MQ=255)                      aCTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTCAGACGCTGATGCTCACGCTCGATATTCTCAAGTTtc                                                   <  1:767840/81‑1 (MQ=255)                       cTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTCAGACGCTGATGCTCACGCTCGATATTCTCAAGTTtct                                                  >  2:767841/1‑81 (MQ=255)                          cGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTCAGACGCTGATGCTCACGCTCGATATTCTCAAGTTtctc                                                 <  2:767843/79‑1 (MQ=255)                          cGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTCAGACGCTGATGCTCACGCTCGATATTCTCAAGTTtctc                                                 <  1:767842/79‑1 (MQ=255)                          cGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTCAGACGCTGATGCTCACGCTCGATATTCTCAAGTTTCTCGt                                               >  2:767900/1‑81 (MQ=255)                           ggCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTCAGACGCTGATGCTCACGCTCGATATTCTCAAGTTTCTCGt                                               >  1:767898/1‑80 (MQ=255)                                 aGCTTCCGCCGACGGGGTTACCCAGGTCGATTTCAGACGCTGATGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAgcg                                        >  1:767888/1‑81 (MQ=255)                                         ccgACGGGGTTACCCAGGTCGATTTCAGACGCTGATGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAAc                                <  2:767844/81‑1 (MQ=255)                                                   tACCCAGGTCGATTTCAGACGCTGATGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTCGTCATc                      >  2:767892/1‑81 (MQ=255)                                                     cccAGGTCGATTTCAGACGCTGATGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTCGTCATcca                    <  1:767846/81‑1 (MQ=255)                                                     cccAGGTCGATTTCAGACGCTGATGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTCGTCATcc                     <  2:767845/80‑1 (MQ=255)                                                     cccAGGTCGATTTCAGACGCTGATGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTCGTCATcc                     <  2:767847/80‑1 (MQ=255)                                                       cAGGTCGATTTCAGACGCTGATGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTCGTCATccac                   >  2:767905/1‑80 (MQ=255)                                                       cAGGTCGATCTCAGACGCTGATGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTCGTCATccacc                  <  2:767848/81‑1 (MQ=255)                                                        aGGTCGATTTCAGACGCTGATGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTCGTCATccacca                 <  1:767849/81‑1 (MQ=255)                                                             gATTTCAGACGCTGATGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTCGTCATCCACCAGGccc            <  2:767850/81‑1 (MQ=255)                                                              aTTTCAGACGCTGATGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTCGTCATCCACCAGGcc             >  2:767891/1‑79 (MQ=255)                                                                       gctgatgctCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTCGTCATCCACCAGGCCCAGTTCACGAc  >  1:767904/1‑81 (MQ=255)                                                                            |                                                                            GGCGCAGTCAGGTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTCAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTCGTCATCCACCAGGCCCAGTTCACGAC  >  NC_000913/3924129‑3924279

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑