Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,020,179	C→T	R186C (CGT→TGT)  noncoding (202/252 nt)	ubiJ → esrE →	ubiquinone biosynthesis accessory factor UbiJ small RNA EsrE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,020,179	0	C	T	100.0%	130.6 / NA	36	R186C (CGT→TGT)  noncoding (202/252 nt)	ubiJ esrE	ubiquinone biosynthesis accessory factor UbiJ small RNA EsrE
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (19/17);  total (19/17)

CATTACTGAAGAGTGGCGTATGGCACCCGGTCCGCTTGAAGTGGCCTGGTTTGCGGAAGAGACGGCTGCCGTCGAGCGTGCTGTTGATGCCCTGACCAAACGGCTGGAAAAACTGGAGGCTAAATGACGCCAGGTGAAGTACGGCGCCTATAT  >  NC_000913/4020103‑4020255                                                                             |                                                                             cATTACTGAAGAGTGGCGTATGGCACCCGGTCCGCTTGAAGTGGCCTGGTTTGCGGAAGAGACGGCTGCCGTCGAGTGTGc                                                                          >  2:791000/1‑81 (MQ=255)  aTTACTGAAGAGTGGCGTATGGCACCCGGTCCGCTTGAAGTGGCCTGGTTTGCGGAAGAGACGGCTGCCGTCGAGTGTGCt                                                                         <  2:790958/81‑1 (MQ=255)        gAAGAGTGGCGTATGGCACCCGGTCCGCTTGAAGTGGCCTGGTTTGCGGAAGAGACGGCTGCCGTCGAGTGTGCTGTTGAt                                                                   >  2:791007/1‑81 (MQ=255)         aaGAGTGGCGTATGGCACCCGGTCCGCTTGAAGTGGCCTGGTTTGCGGAAGAGACGGCTGCCGTCGAGTGTGCTGTTGAt                                                                   <  2:790959/80‑1 (MQ=255)            agTGGCGTATGGCACCCGGTCCGCTTGAAGTGGCCTGGTTTGCGGAAGAGACGGCTGCCGTCGAGTGTGCTGTTGATGccc                                                               >  1:790999/1‑81 (MQ=255)             gTGGCGTATGGCACCCGGTCCGCTTGAAGTGGCCTGGTTTGCGGAAGAGACGGCTGCCGTCGAGTGTGCTGTTGATGCCCt                                                              >  1:791017/1‑81 (MQ=255)                  gTATGGCACCCGGTCCGCTTGAAGTGGCCTGGTTTGCGGAAGAGACGGCTGCCGTCGAGTGTGCTGTTGATGCCCTGACCa                                                         <  1:790960/81‑1 (MQ=255)                    aTGGCACCCGGTCCGCTTGAAGTGGCCTGGTTTGCGGAAGAGACGGCTGCCGTCGAGTGTGCTGTTGATGCCCTGACCaaa                                                       >  2:791027/1‑81 (MQ=255)                      ggCACCCGGTCCGCTTGAAGTGGCCTGGTTTGCGGAAGAGACGGCTGCCGTCGAGTGTGCTGTTGATGCCCTGACCAAACg                                                     >  2:791019/1‑81 (MQ=255)                        cACCCGGTCCGCTTGAAGTGGCCTGGTTTGCGGAAGAGACGGCTGCCGTCGAGTGTGCTGTTGATGCCCTGACCAAACGGc                                                   <  2:790961/81‑1 (MQ=255)                           ccGGTCCGCTTGAAGTGGCCTGGTTTGCGGAAGAGACGGCTGCCGTCGAGTGTGCTGTTGATGCCCTGACCAAACGGCTg                                                 >  1:791022/1‑80 (MQ=255)                                 cgattGAAGTGGCCTGGTTTGCGGAAGAGACGGCTGCCGTCGAGTGTGCTGTTGATGCCCTGACCAAACGGCTGGAAAAAc                                          <  2:790963/78‑1 (MQ=255)                                 cGCTTGAAGTGGCCTGGTTTGCGGAAGAGACGGCTGCCGTCGAGTGTGCTGTTGATGCCCTGACCAAACGGCTGGAAAAAc                                          <  1:790962/81‑1 (MQ=255)                                 cGCTTGAAGTGGCCTGGTTTGCGGAAGAGACGGCTGCCGTCGAGTGTGCTGTTGATGCCCTGACCAAACGGCTGGAAAAAc                                          <  2:790964/81‑1 (MQ=255)                                 cGCTTGAAGTGGCCTGGTTTGCGGAAGAGACGGCTGCCGTCGAGTGTGCTGTTGATGCCCTGACCAAACGGCTGGAAAAAc                                          >  1:791025/1‑81 (MQ=255)                                  gCTTGAAGTGGCCTGGTTTGCGGAAGAGACGGCTGCCGTCGAGTGTGCTGTTGATGCCCTGACCAAACGGCTGGAAAAACt                                         <  1:790965/81‑1 (MQ=255)                                  gCTTGAAGTGGCCTGGTTTGCGGAAGAGACGGCTGCCGTCGAGTGTGCTGTTGATGCCCTGACCAAACGGCTGGAAAAACt                                         <  1:790966/81‑1 (MQ=255)                                  gCTTGAAGTGGCCTGGTTTGCGGAAGAGACGGCTGCCGTCGAGTGTGCTGTTGATGCCCTGACCAAACGGCTGGAAAAACt                                         >  1:791004/1‑81 (MQ=255)                                   cTTGAAGTGGCCTGGTTTGCGGAAGAGACGGCTGCCGTCGAGTGTGCTGTTGATGCCCTGACCAAACGGCTGGAAAAACTg                                        <  1:790967/81‑1 (MQ=255)                                            gcttGGTTTGCGGAAGAGACGGCTGCCGTCGAGTGTGCTGTTGATGCCCTGACCAAACGGCTGGAAAAACTGGAGGCTaaa                               >  2:791005/4‑81 (MQ=255)                                               tGGTTTGCGGAAGAGACGGCTGCCGTCGAGTGTGCTGTTGATGCCCTGACCAAACGGCTGGAAAAACTGGAGGCTAAATGa                            >  1:791023/1‑81 (MQ=255)                                                ggTTTGCGGAAGAGACGGCTGCCGTCGAGTGTGCTGTTGATGCCCTGACCAAACGGCTGGAAAAACTGGAGGCTAAATGAc                           >  1:791024/1‑81 (MQ=255)                                                 gTTTGCGGAAGAGACGGCTGCCGTCGAGTGTGCTGTTGATGCCCTGACCAAACGGCTGGAAAAACTGGAGGCTAAATGAc                           <  1:790968/80‑1 (MQ=255)                                                 gTTTGCGGAAGAGACGGCTGCCGTCGAGTGTGCTGTTGATGCCCTGACCAAACGGCTGGAAAAACTGGAGGCTAAATGACg                          >  2:791013/1‑81 (MQ=255)                                                   ttGCGGAAGAGACGGCTGCCGTCGAGTGTGCTGTTGATGCCCTGACCAAACGGCTGGAAAAACTGGAGGCTAAATGACGc                         >  2:791011/1‑80 (MQ=255)                                                      cGGAAGAGACGGCTGCCGTCGAGTGTGCTGTTGATGCCCTGACCAAACGGCTGGAAAAACTGGAGGCTAAATGACGCCAg                      <  1:790969/80‑1 (MQ=255)                                                       ggAAGAGACGGCTGCCGTCGAGTGTGCTGTTGATGCCCTGACCAAACGGCTGGAAAAACTGGAGGCTAAATGACGCCAGGt                    >  2:791015/1‑81 (MQ=255)                                                         aaGAGACGGCTGCCGTCGAGTGTGCTGTTGATGCCCTGACCAAACGGCTGGAAAAACTGGAGGCTAAATGACGCCAGGTg                   >  1:791031/1‑80 (MQ=255)                                                         aaGAGACGGCTGCCGTCGAGTGTGCTGTTGATGCCCTGACCAAACGGCTGGAAAAACTGGAGGCTAAATGACGCCAGGTg                   >  2:791008/1‑80 (MQ=255)                                                             gaCGGCTGCCGTCGAGTGTGCTGTTGATGCCCTGACCAAACGGCTGGAAAAACTGGAGGCTAAATGACGCCAGGTGAAGTa              <  2:790970/81‑1 (MQ=255)                                                              aCGGCTGCCGTCGAGTGTGCTGTTGATGCCCTGACCAAACGGCTGGAAAAACTGGAGGCTAAATGACGCCAGGTGAAGTAc             <  1:790971/81‑1 (MQ=255)                                                               cGGCTGCCGTCGAGTGTGCTGTTGATGCCCTGACCAAACGGCTGGAAAAACTGGAGGCTAAATGACGCCAGGTGAAGTACg            <  2:790972/81‑1 (MQ=255)                                                                ggCTGCCGTCGAGTGTGCTGTTGATGCCCTGACCAAACGGCTGGAAAAACTGGAGGCTAAATGACGCCAGGTGAAGTACgg           <  1:790973/81‑1 (MQ=255)                                                                    gCCGTCGAGTGTGCTGTTGATGCCCTGACCAAACGGCTGGAAAAACTGGAGGCTAAATGACGCCAGGTGAAGTACGGCGcc       >  1:791010/1‑81 (MQ=255)                                                                     ccGTCGAGTGTGCTGTTGATGCCCTGACCAAACGGCTGGAAAAACTGGAGGCTAAATGACGCCAGGTGAAGTACGgcgc        <  2:790974/79‑1 (MQ=255)                                                                         cGAGTGTGCTGTTGATGCCCTGACCAAACGGCTGGAAAAACTGGAGGCTAAATGACGCCAGGTGAAGTACGGCGCCTatat  >  1:791028/1‑81 (MQ=255)                                                                             |                                                                             CATTACTGAAGAGTGGCGTATGGCACCCGGTCCGCTTGAAGTGGCCTGGTTTGCGGAAGAGACGGCTGCCGTCGAGCGTGCTGTTGATGCCCTGACCAAACGGCTGGAAAAACTGGAGGCTAAATGACGCCAGGTGAAGTACGGCGCCTATAT  >  NC_000913/4020103‑4020255

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑