Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,033,383	T→A	L80Q (CTG→CAG)	trkH →	K(+) transporter TrkH

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,033,383	0	T	A	96.8%	103.9 / ‑4.6	31	L80Q (CTG→CAG)	trkH	K(+) transporter TrkH
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base A (13/17);  minor base G (0/1);  total (13/18)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

CGAACCGCAAAGAGAAAGGCGAACTTAAATCCCGTGAGGGGTTTCTGATAGTGGTGCTGTTCTGGACCGTGCTGGGTAGCGTCGGTGCGCTCCCTTTTATCTTCTCGGAAAGCCCGAACCTCACGATTACCGATGCGTTTTTT  >  NC_000913/4033311‑4033453                                                                         |                                                                       cGAACCGCAAAGAGAAAGGCGAACTTAAATCCCGTGAGGGGTTTCTGATAGTGGTGCTGTTCTGGACCGTGCAGGGTAGCg                                                                >  2:794360/1‑81 (MQ=255)   aaCCGCAAAGAGAAAGGCGAACTTAAATCCCGTGAGGGGTTTCTGATAGTGGTGCTGTTCTGGACCGTGCAGGGTAGCGTc                                                              <  1:794312/81‑1 (MQ=255)   aaCCGCAAAGAGAAAGGCGAACTTAAATCCCGTGAGGGGTTTCTGATAGTGGTGCTGTTCTGGACCGTGCAGGGTAGCGTc                                                              <  2:794313/81‑1 (MQ=255)   aaCCGCAAAGAGAAAGGCGAACTTAAATCCCGTGAGGGGTTTCTGATAGTGGTGCTGTTCTGGACCGTGCAGGGTAGCGTc                                                              <  1:794311/81‑1 (MQ=255)         aaaGAGAAAGGCGAACTTAAATCCCGTGAGGGGTTTCTGATAGTGGTGCTGTTCTGGACCGTGCAGGGTAGCGTCGGTgcg                                                        >  1:794361/1‑81 (MQ=255)         aaaGAGAAAGGCGAACTTAAATCCCGTGAGGGGTTTCTGATAGTGGTGCTGTTCTGGACCGTGCAGGGTAGCGTCGGTgcg                                                        >  2:794350/1‑81 (MQ=255)          aaGAGAAAGGCGAACTTAAATCCCGTGAGGGGTTTCTGATAGTGGTGCTGTTCTGGACCGTGCAGGGTAGCGTCGGTgcgc                                                       <  1:794314/81‑1 (MQ=255)            gagaAAGGCGAACTTAAATCCCGTGAGGGGTTTCTGATAGTGGTGCTGTTCTGGACCGTGCAGGGTAGCGTCGGTGCGCTc                                                     >  1:794362/1‑81 (MQ=255)               aaaGGCGAACTTAAATCCCGTGAGGGGTTTCTGATAGTGGTGCTGTTCTGGACCGTGCAGGGTAGCGTCGGTGCGCTCCCt                                                  >  1:794351/1‑81 (MQ=255)               aaaGGCGAACTTAAATCCCGTGAGGGGTTTCTGATAGTGGTGCTGTTCTGGACCGTGCAGGGTAGCGTCGGTGCGCTCCCt                                                  >  2:794352/1‑81 (MQ=255)               aaaGGCGAACTTAAATCCCGTGAGGGGTTTCTGATAGTGGTGCTGTTCTGGACCGTGCAGGGTAGCGTCGGTGCGCTCCCt                                                  <  1:794315/81‑1 (MQ=255)                 aGGCGAACTTAAATCCCGTGAGGGGTTTCTGATAGTGGTGCTGTTCTGGACCGTGCAGGGTAGCGTCGGTGCGCTCCCttt                                                <  1:794316/81‑1 (MQ=255)                     gAACTTAAATCCCGTGAGGGGTTTCTGATAGTGGTGCTGTTCTGGACCGTGCAGGGTAGCGTCGGTGCGCTCCCTTTTAtc                                            <  2:794317/81‑1 (MQ=255)                           aaaTCCCGTGGGGGGTTTCTGATAGTGGTGCTGTTCTGGACCGTGCAGGGTAGCGTCGGTGCGCTCCCTTTTATCTtctc                                       <  2:794318/80‑1 (MQ=255)                           aaaTCCCGTGAGGGGTTTCTGATAGTGGTGCTGTTCTGGACCGTGCAGGGTAGCGTCGGTGCGCTCCCTTTTAtcttct                                        <  2:794319/79‑1 (MQ=255)                            aaTCCCGTGAGGGGTTTCTGATAGTGGTGCTGTTCTGGACCGTGCAGGGTAGCGTCGGTGCGCTCCCTTTTATCTTCTCgg                                     <  1:794320/81‑1 (MQ=255)                            aaTCCCGTGAGGGGTTTCTGATAGTGGTGCTGTTCTGGACCGTGCAGGGTAGCGTCGGTGCGCTCCCTTTTATCTTCTCg                                      >  1:794355/1‑80 (MQ=255)                                  gTGAGGGGTTTCTGATAGTGGTGCTGTTCTGGACCGTGCAGGGTAGCGTCGGTGCGCTCCCTTTTATCTTCTCGGAAAGcc                               >  1:794363/1‑81 (MQ=255)                                         gTTTCTGATAGTGGTGCTGTTCTGGACCGTGCAGGGTAGCGTCGGTGCGCTCCCTTTTATCTTCTCGGAAAGCCCGAACCt                        >  1:794371/1‑81 (MQ=255)                                          tttCTGATAGTGGTGCTGTTCTGGACCGTGCGGGGTAGCGTCGGTGCGCTCCCTTTTATCTTCTCGGAAAGCCCGAACCTc                       <  2:794321/81‑1 (MQ=255)                                          tttCTGATAGTGGTGCTGTTCTGGACCGTGCAGGGTAGCGTCGGTGCGCTCCCTTTTATCTTCTCGGAAAGCCCGAACCt                        >  2:794370/1‑80 (MQ=255)                                                   gtggtgCTGTTCTGGACCGTGCAGGGTAGCGTCGGTGCGCTCCCTTTTATCTTCTCGGAAAGCCCGAACCTCACGATTAcc              <  2:794323/81‑1 (MQ=255)                                                   gtggtgCTGTTCTGGACCGTGCAGGGTAGCGTCGGTGCGCTCCCTTTTATCTTCTCGGAAAGCCCGAACCTCACGATTAcc              <  1:794322/81‑1 (MQ=255)                                                      gtgCTGTTCTGGACCGTGCAGGGTAGCGTCGGTGCGCTCCCTTTTATCTTCTCGGAAAGCCCGAACCTCACGATTACCGAt           <  1:794324/81‑1 (MQ=255)                                                         cTGTTCTGGACCGTGCAGGGTAGCGTCGGTGCGCTCCCTTTTATCTTCTTGGAAAGCCCGAACCTCACGATTACCGATGCg        >  2:794374/1‑81 (MQ=255)                                                         cTGTTCTGGACCGTGCAGGGTAGCGTCGGTGCGCTCCCTTTTATCTTCTCGGAAAGCCCGAACCTCACGATTACCGATGc         <  1:794325/80‑1 (MQ=255)                                                           gTTCTGGACCGTGCAGGGTAGCGTCGGTGCGCTCCCTTTTATCTTCTCGGAAAGCCCGAACCTCACGATTACCGATGCGtt      >  1:794356/1‑81 (MQ=255)                                                           gTTCTGGACCGTGCAGGGTAGCGTCGGTGCGCTCCCTTTTATCTTCTCGGAAAGCCCGAACCTCACGATTACCGATGCGtt      >  2:794359/1‑81 (MQ=255)                                                            ttCTGGACCGTGCAGGGTAGCGTCGGTGCGCTCCCTTTTATCTTCTCGGAAAGCCCGAACCTCACGATTACCGATGCGttt     <  2:794326/81‑1 (MQ=255)                                                                 gACCGTGCAGGGTAGCGTCGGTGCGCTCCCTTTTATCTTCTCGGAAAGCCCGAACCTCACGATTACCGATGCGtttttt  <  2:794328/79‑1 (MQ=255)                                                                 gACCGTGCAGGGTAGCGTCGGTGCGCTCCCTTTTATCTTCTCGGAAAGCCCGAACCTCACGATTACCGATGCGttttt   <  2:794327/78‑1 (MQ=255)                                                                         |                                                                       CGAACCGCAAAGAGAAAGGCGAACTTAAATCCCGTGAGGGGTTTCTGATAGTGGTGCTGTTCTGGACCGTGCTGGGTAGCGTCGGTGCGCTCCCTTTTATCTTCTCGGAAAGCCCGAACCTCACGATTACCGATGCGTTTTTT  >  NC_000913/4033311‑4033453

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑