Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,041,630	A→G	W124R (TGG→CGG)	mobA ←	molybdenum cofactor guanylyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,041,630	0	A	G	100.0%	101.6 / NA	28	W124R (TGG→CGG)	mobA	molybdenum cofactor guanylyltransferase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (18/10);  total (18/10)

GTAATAAAGGCTCAATAGCGCGGTTTACCAGAGCAATAGTCGGGTGATCGCGTTCACCGTCATGGACCCACACGACAGGCGCATCTTTGCGCTGATGATTAAGCCTGGCTGCTAAATCAGGGGGAATGTAAGGCGTATCGCACGGG  >  NC_000913/4041561‑4041706                                                                      |                                                                             gTAATAAAGGCTCAATAGCGCGGTTTACCAGAGCAATAGTCGGGTGATCGCGTTCACCGTCATGGACCCGCACGACAGgcg                                                                   >  1:796180/1‑81 (MQ=255)   aataaAGGCTCAATAGCGCGGTTTACCAGAGCAATAGTCGGGTGATCGCGTTCACCGTCATGGACCCGCACGACAGGCGCa                                                                 >  2:796184/1‑81 (MQ=255)       aaGGCTCAATAGCGCGGTTTACCAGAGCAATAGTCGGGTGATCGCGTTCACCGTCATGGACCCGCACGACAGGCGCATCt                                                              <  1:796143/80‑1 (MQ=255)            tCAATAGCGCGGTTTACCAGAGCAATAGTCGGGTGATCGCGTTCACCGTCATGGACCCGCACGACAGGCGCATCTTTgcgc                                                        >  2:796197/1‑81 (MQ=255)            tCAATAGCGCGGTTTACCAGAGCAATAGTCGGGTGATCGCGTTCACCGTCATGGACCCGCACGACAGGCGCATCTTTgcgc                                                        >  1:796178/1‑81 (MQ=255)             cAATAGCGCGGTTTACCAGAGCAATAGTCGGGTGATCGCGTTCACCGTCATGGACCCGCACGACAGGCGCATCTTTGCGCt                                                       >  1:796207/1‑81 (MQ=255)               aTAGCGCGGTTTACCAGAGCAATAGTCGGGTGATCGCGTTCACCGTCATGGACCCGCACGACAGGCGCATCTTTGCGCtga                                                     <  1:796144/81‑1 (MQ=255)                  gcgcGGTTTACCAGAGCAATAGTCGGGTGATCGCGTTCACCGTCATGGACCCGCACGACAGGCGCATCTTTGCGCtgatga                                                  >  1:796182/1‑81 (MQ=255)                          tACCAGAGCAATAGTCGGGTGATCGCGTTCACCGTCATGGACCCGCACGACAGGCGCATCTTTGCGCTGATGATTAAGCCt                                          >  2:796186/1‑81 (MQ=255)                            ccAGAGCAATAGTCGGGTGATCGCGTTCACCGTCATGGACCCGCACGACAGGCGCATCTTTGCGCTGATGATTAAGCCTgg                                        <  2:796145/81‑1 (MQ=255)                            ccAGAGCAATAGTCGGGTGATCGCGTTCACCGTCATGGACCCGCACGACAGGCGCATCTTTGCGCTGATGATTAAGCCTg                                         <  2:796146/80‑1 (MQ=255)                               gagCAATAGTCGGGTGATCGCGTTCACCGTCATGGACCCGCACGACAGGCGCATCTTTGCGCTGATGATTAAGCCTGgctg                                     <  1:796147/81‑1 (MQ=255)                                  cAATAGTCGGGTGATCGCGTTCACCGTCATGGACCCGCACGACAGGCGCATCTTTGCGCTGATGATTAAGCCTGgctgct                                   >  1:796181/1‑80 (MQ=255)                                  cAATAGTCGGGTGATCGCGTTCACCGTCATGGACCCGCACGACAGGCGCATCTTTGCGCTGATGATTAAGCCTGGCTGCTa                                  <  1:796148/81‑1 (MQ=255)                                           ggTGATCGCGTTCACCGTCATGGACCCGCACGACAGGCGCATCTTTGCGCTGATGATTAAGCCTGGCTGCTAAATCAgggg                         >  1:796198/1‑81 (MQ=255)                                               aTCGCGTTCACCGTCATGGACCCGCACGACAGGCGCATCTTTGCGCTGATGATTAAGCCTGGCTGCTAAATCAGGGGGAAt                     >  1:796199/1‑81 (MQ=255)                                                  gcgTTCACCGTCATGGACCCGCACGACAGGCGCATCTTTGCGCTGATGATTAAGCCTGGCTGCTAAATCAGGGGGAATGt                   >  2:796179/1‑80 (MQ=255)                                                        aCCGTCATGGACCCGCACGACAGGCGCATCTTTGCGCTGATGATTAAGCCTGGCTGCTAAATCAGGGGGAATGTAAGGCg             >  2:796195/1‑80 (MQ=255)                                                        aCCGTCATGGACCCGCACGACAGGCGCATCTTTGCGCTGATGATTAAGCCTGGCTGCTAAATCAGGGGGAATGTAAGGCGt            <  2:796149/81‑1 (MQ=255)                                                        aCCGTCATGGACCCGCACGACAGGCGCATCTTTGCGCTGATGATTAAGCCTGGCTGCTAAATCAGGGGGAATGTAAGGCGt            <  2:796150/81‑1 (MQ=255)                                                           gTCATGGACCCGCACGACAGGCGCATCTTTGCGCTGATGATTAAGCCTGGCTGCTAAATCAGGGGGAATGTAAGGCGTATc         >  1:796187/1‑81 (MQ=255)                                                             cATGGACCCGCACGACAGGCGCATCTTTGCGCTGATGATTAAGCCTGGCTGCTAAATCAGGGGGAATGTAAGGCGTATCg        >  2:796201/1‑80 (MQ=255)                                                             cATGGACCCGCACGACAGGCGCATCTTTGCGCTGATGATTAAGCCTGGCTGCTAAATCAGGGGGAATGTAAGGCGTATCg        >  2:796202/1‑80 (MQ=255)                                                              aTGGACCCGCACGACAGGCGCATCTTTGCGCTGATGATTAAGCCTGGCTGCTAAATCAGGGGGAATGTAAGGCGTATCGc       <  1:796151/80‑1 (MQ=255)                                                              aTGGACCCGCACGACAGGCGCATCTTTGCGCTGATGATTAAGCCTGGCTGCTAAATCAGGGGGAATGTAAGGCGTATCGCa      >  2:796191/1‑81 (MQ=255)                                                               tGGACCCGCACGACAGGCGCATCTTTGCGCTGATGATTAAGCCTGGCTGCTAAATCAGGGGGAATGTAAGGCGTATCGCAc     <  1:796152/81‑1 (MQ=255)                                                                ggACCCGCACGACAGGCGCATCTTTGCGCTGATGATTAAGCCTGGCTGCTAAATCAGGGGGAATGTAAGGCGTATCGCACg    >  1:796215/1‑81 (MQ=255)                                                                  aCCCGCACGACAGGCGCATCTTTGCGCTGATGATTAAGCCTGGCTGCTAAATCAGGGGGAATGTAAGGCGTATCGCACggg  >  2:796204/1‑81 (MQ=255)                                                                      |                                                                             GTAATAAAGGCTCAATAGCGCGGTTTACCAGAGCAATAGTCGGGTGATCGCGTTCACCGTCATGGACCCACACGACAGGCGCATCTTTGCGCTGATGATTAAGCCTGGCTGCTAAATCAGGGGGAATGTAAGGCGTATCGCACGGG  >  NC_000913/4041561‑4041706

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑