Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	397,213	A→T	Q192L (CAG→CTG)	sbmA →	peptide antibiotic/peptide nucleic acid transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	397,213	0	A	T	100.0%	113.1 / NA	31	Q192L (CAG→CTG)	sbmA	peptide antibiotic/peptide nucleic acid transporter
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (11/20);  total (11/20)

GTACAGCGATGAACGAATATTACATGGCGAACTGGCAACAACTGCGTCATATCGAAGGGGCCGCACAGCGTGTGCAGGAAGACACCATGCGTTTTGCTTCAACGCTGGAGAATATGGGCGTCAGTTTTATCAACGCCATCATGACGTT  >  NC_000913/397138‑397285                                                                            |                                                                         gTACAGCGATGAACGAATATTACATGGCGAACTGGCAACAACTGCGTCATATCGAAGGGGCCGCACAGCGTGTGCTGGAAg                                                                     <  2:84797/81‑1 (MQ=255)     aGCGATGAACGAATATTACATGGCGAACTGGCAACAACTGCGTCATATCGAAGGGGCCGCACAGCGTGTGCTGGAAGacac                                                                 <  1:84798/81‑1 (MQ=255)     aGCGATGAACGAATATTACATGGCGAACTGGCAACAACTGCGTCATATCGAAGGGGCCGCACAGCGTGTGCTGGAAGacac                                                                 >  1:84848/1‑81 (MQ=255)      gCGATGAACGAATATTACATGGCGAACTGGCAACAACTGCGTCATATCGAAGGGGCCGCACAGCGTGTGCTGGAAGacac                                                                 >  1:84846/1‑80 (MQ=255)       cGATGAACGAATATTACATGGCGAACTGGCAACAACTGCGTCATATCGAAGGGGCCGCACAGCGTGTGCTGGAAGACACCa                                                               <  1:84799/81‑1 (MQ=255)          tGAACGAATATTACATGGCGAACTGGCAACAACTGCGTCATATCGAAGGGGCCGCACAGCGTGTGCTGGAAGACACCATGc                                                            <  1:84800/81‑1 (MQ=255)                aaTATTACATGGCGAACTGGCAACAACTGCGTCATATCGAAGGGGCCGCACAGCGTGTGCTGGAAGACACCATGCGTTTTg                                                      <  2:84801/81‑1 (MQ=255)                     tACATGGCGAACTGGCAACAACTGCGTCATATCGAAGGGGCCGCACAGCGTGTGCTGGAAGACACCATGCGTTTTGCTTc                                                  >  2:84871/1‑80 (MQ=255)                          ggCGAACTGGCAACAACTGCGTCATATCGAAGGGGCCGCACAGCGTGTGCTGGAAGACACCATGCGTTTTGCTTCAACGCt                                            >  2:84860/1‑81 (MQ=255)                           gCGAACTGGCAACAACTGCGTCATATCGAAGGGGCCGCACAGCGTGTGCTGGAAGACACCATGCGTTTTGCTTCAACGCt                                            <  2:84802/80‑1 (MQ=255)                           gCGAACTGGCAACAACTGCGTCATATCGAAGGGGCCGCACAGCGTGTGCTGGAAGACACCATGCGTTTTGCTTCAACGCTg                                           <  1:84803/81‑1 (MQ=255)                            cGAACTGGCAACAACTGCGTCATATCGAAGGGGCCGCACAGCGTGTGCTGGAAGACACCATGCGTTTTGCTTCAACGCTg                                           >  2:84850/1‑80 (MQ=255)                             gAACTGGCAACAACTGCGTCATATCGAAGGGGCCGCACAGCGTGTGCTGGAAGACACCATGCGTTTTGCTTCAACGCTg                                           >  1:84864/1‑79 (MQ=255)                             gAACTGGCAACAACTGCGTCATATCGAAGGGGCCGCACAGCGTGTGCTGGAAGACACCATGCGTTTTGCTTCAACGCTGga                                         <  2:84804/81‑1 (MQ=255)                                   gCAACAACTGCGTCATATCGAAGGGGCCGCACAGCGTGTGCTGGAAGACACCATGCGTTTTGCTTCAACGCTGGAGAatat                                   <  2:84805/81‑1 (MQ=255)                                     aacaacTGCGTCATATCGAAGGGGCCGCACAGCGTGTGCTGGAAGACACCATGCGTTTTGCTTCAACGCTGGAGAATATgg                                 >  1:84858/1‑81 (MQ=255)                                      acaacTGCGTCATATCGAAGGGGCCGCACAGCGTGTGCTGGAAGACACCATGCGTTTTGCTTCAACGCTGGAGAATATggg                                >  2:84856/1‑81 (MQ=255)                                       caacTGCGTCATATCGAAGGGGCCGCACAGCGTGTGCTGGAAGACACCATGCGTTTTGCTTCAACGCTGGAGAATATGGGc                               <  1:84806/81‑1 (MQ=255)                                          cTGCGTCATATCGAAGGGGCCGCACAGCGTGTGCTGGAAGACACCATGCGTTTTGCTTCAACGCTGGAGAATATGGGCGTc                            <  1:84807/81‑1 (MQ=255)                                             cGTCATATCGAAGGGGCCGCACAGCGTGTGCTGGAAGACACCATGCGTTTTGCTTCAACGCTGGAGAATATGGGCGTCAGt                         <  1:84808/81‑1 (MQ=255)                                                  tatCGAAGGGGCCGCACAGCGTGTGCTGGAAGACACCATGCGTTTTGCTTCAACGCTGGAGAATATGGGCGTCAGTTTTAt                    <  2:84809/81‑1 (MQ=255)                                                    tCGAAGGGGCCGCACAGCGTGTGCTGGAAGACACCATGCGTTTTGCTTCAACGCTGGAGAATATGGGCGTCAGTTTTATCa                  <  2:84810/81‑1 (MQ=255)                                                      gAAGGGGCCGCACAGCGTGTGCTGGAAGACACCATGCGTTTTGCTTCAACGCTGGAGAATATGGGCGTCAGTTTTATCAAc                <  2:84811/81‑1 (MQ=255)                                                      gAAGGGGCCGCACAGCGTGTGCTGGAAGACACCATGCGTTTTGCTTCAACGCTGGAGAATATGGGCGTCAGTTTTATCAAc                >  2:84862/1‑81 (MQ=255)                                                           ggCCGCACAGCGTGTGCTGGAAGACACCATGCGTTTTGCTTCAACGCTGGAGAATATGGGCGTCAGTTTTATCAACGCcat           <  2:84812/81‑1 (MQ=255)                                                           ggCCGCACAGCGTATGCTGGAAGACACCATGCGTTTTGCTTCAACGCTGGAGAATATGGGCGTCAGTTTTATCAACGCcat           <  2:84813/81‑1 (MQ=255)                                                              cGCACAGCGTGTGCTGGAAGACACCATGCGTTTTGCTTCAACGCTGGAGAATATGGGCGTCAGTTTTATCAACGCcatcat        >  1:84865/1‑81 (MQ=255)                                                              cGCACAGCGTGTGCTGGAAGACACCATGCGTTTTGCTTCAACGCTGGAGAATATGGGCGTCAGTTTTATCAACGCcatcat        <  1:84814/81‑1 (MQ=255)                                                               gCACAGCGTGTGCTGGAAGACACCATGCGTTTTGCTTCAACGCTGGAGAATATGGGCGTCAGTTTTATCAACGCCATCATg       <  2:84815/81‑1 (MQ=255)                                                                    gCGTGTGCTGGAAGACACCATGCGTTTTGCTTCAACGCTGGAGAATATGGGCGTCAGTTTTATCAACGCCATCATGACGtt  <  2:84816/81‑1 (MQ=255)                                                                     cGTGTGCTGGAAGACACCATGCGTTTTGCTTCAACGCTGGAGAATATGGGCGTCAGTTTTATCAACGCCATCATGACGtt  >  2:84868/1‑80 (MQ=255)                                                                            |                                                                         GTACAGCGATGAACGAATATTACATGGCGAACTGGCAACAACTGCGTCATATCGAAGGGGCCGCACAGCGTGTGCAGGAAGACACCATGCGTTTTGCTTCAACGCTGGAGAATATGGGCGTCAGTTTTATCAACGCCATCATGACGTT  >  NC_000913/397138‑397285

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑