Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,081,536	A→C	F109V (TTC→GTC)	fdoI ←	formate dehydrogenase O subunit gamma

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,081,536	0	A	C	100.0%	128.1 / NA	35	F109V (TTC→GTC)	fdoI	formate dehydrogenase O subunit gamma
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (15/20);  total (15/20)

AGATAATCACGCCGCTCACCAGCAGCAGAACCAGGAAAATAATCGCCGCCCAGAAAACGCATTTCTGACCGAAGTTATAACGCCCGGTGTCACCTACTTCCTCGTTGACGACGATCTTACGAATATTCTTCGCCCAA  >  NC_000913/4081464‑4081600                                                                         |                                                                 aGATAATCACGCCGCTCACCAGCAGCAGAACCAGGAAAATAATCGCCGCCCAGAAAACGCATTTCTGACCGACGTTATAAc                                                          <  2:805163/81‑1 (MQ=255)    tAATCACGCCGCTCACCAGCAGCAGAACCAGGAAAATAATCGCCGCCCAGAAAACGCATTTCTGACCGACGTTATAACGcc                                                       <  1:805164/81‑1 (MQ=255)      aTCACGCCGCTCACCAGCAGCAGAACCAGGAAAATAATCGCCGCCCAGAAAACGCATTTCTGACCGACGTTATAACGccc                                                      <  2:805165/80‑1 (MQ=255)        cACGCCGCTCACCAGCAGCAGAACCAGGAAAATAATCGCCGCCCAGAAAACGCATTTCTGACCGACGTTATAACGCCCGgt                                                   >  1:805209/1‑81 (MQ=255)         aCGCCGCTCACCAGCAGCAGAACCAGGAAAATAATCGCCGCCCAGAAAACGCATTTCTGACCGACGTTATAACGCCCGgtg                                                  >  1:805210/1‑81 (MQ=255)              gcTCACCAGCAGCAGAACCAGGAAAATAATCGCCGCCCAGAAAACGCATTTCTGACCGACGTTATAACGCCCGGTGTCAcc                                             >  2:805198/1‑81 (MQ=255)               cTCACCAGCAGCAGAACCAGGAAAATAATCGCCGCCCAGAAAACGCATTTCTGACCGACGTTATAACGCCCGGTGTCACCt                                            <  1:805166/81‑1 (MQ=255)                  aCCAGCAGCAGAACCAGGAAAATAATCGCCGCCCAGAAAACGCATTTCTGACCGACGTTATAACGCCCGGTGTCACCTACt                                         >  2:805206/1‑81 (MQ=255)                     agcagcagAACCAGGAAAATAATCGCCGCCCAGAAAACGCATTTCTGACCGACGTTATAACGCCCGGTGTCACCTACTTcc                                      <  2:805167/81‑1 (MQ=255)                       cagcagAACCAGGAAAATAATCGCCGCCCAGAAAACGCATTTCTGACCGACGTTATAACGCCCGGTGTCACCTACTTCCTc                                    >  2:805230/1‑81 (MQ=255)                       cagcagAACCAGGAAAATAATCGCCGCCCAGAAAACGCATTTCTGACCGACGTTATAACGCCCGGTGTCACCTACTTCCTc                                    <  2:805168/81‑1 (MQ=255)                        agcagAACCAGGAAAATAATCGCCGCCCAGAAAACGCATTTCTGACCGACGTTATAACGCCCGGTGTCACCTACTTCCTCg                                   >  2:805200/1‑81 (MQ=255)                          cagAACCAGGAAAATAATCGCCGCCCAGAAATCGCATTTCTGACCGACGTTATAACGCCCGGTGTCACCTACTTCCTCGtt                                 <  1:805169/81‑1 (MQ=255)                          cagAACCAGGAAAATAATCGCCGCCCAGAAAACGCATTTCTGACCGACGTTATAACGCCCGGTGTCACCTACTTCCTCGtt                                 <  1:805170/81‑1 (MQ=255)                           agAACCAGGAAAATAATCGCCGCCCAGAAAACGCATTTCTGACCGACGTTATAACGCCCGGTGTCACCTACTTCCTCGtt                                 >  2:805220/1‑80 (MQ=255)                             aaCCAGGAAAATAATCGCCGCCCAGAAAACGCATTTCTGACCGACGTTATAACGCCCGGTGTCACCTACTTCCTCGTTgac                              <  1:805171/81‑1 (MQ=255)                                   gAAAATAATCGCCGCCCAGAAAACGCATTTCTGACCGACGTTATAACGCCCGGTGTCACCTACTTCCTCGTTGACGACGAt                        >  1:805201/1‑81 (MQ=255)                                    aaaaTAATCGCCGCCCAGAAAACGCATTTCTGACCGACGTTATAACGCCCGGTGTCACCTACTTCCTCGTTGACGACGATc                       <  2:805172/81‑1 (MQ=255)                                     aaaTAATCGCCGCCCAGAAAACGCATTTCTGACCGACGTTATAACGCCCGGTGTCACCTACTTCCTCGTTGACGACGATCt                      >  1:805240/1‑81 (MQ=255)                                     aaaTAATCGCCGCCCAGAAAACGCATTTCTGACCGACGTTATAACGCCCGGTGTCACCTACTTCCTCGTTGACGACGATCt                      <  1:805173/81‑1 (MQ=255)                                      aataatCGCCGCCCAGAAAACGCATTTCTGACCGACGTTATAACGCCCGGTGTCACCTACTTCCTCGTTGACGACGATCtt                     <  2:805174/81‑1 (MQ=255)                                          atCGCCGCCCAGAAAACGCATTTCTGACCGACGTTATAACGCCCGGTGTCACCTACTTCCTCGTTGACGACGATCTTACGa                 <  2:805175/81‑1 (MQ=255)                                           tCGCCGCCCAGAAAACGCATTTCTGACCGACGTTATAACGCCCGGTGTCACCTACTTCCTCGTTGACGACGATCTTACGaa                >  1:805212/1‑81 (MQ=255)                                           tCGCCGCCCAGAAAACGCATTTCTGACCGACGTTATAACGCCCGGTGTCACCTACTTCCTCGTTGACGACGATCTTACGaa                <  2:805176/81‑1 (MQ=255)                                              ccgccCAGAAAACGCATTTCTGACCGACGTTATAACGCCCGGTGTCACCTACTTCCTCGTTGACGACGATCTTACGAatat             <  1:805177/81‑1 (MQ=255)                                                    aGAAAACGCATTTCTGACCGACGTTATAACGCCCGGTGTCACCTACTTCCTCGTTGACGACGATCTTACGAATATTCTTCg       <  1:805178/81‑1 (MQ=255)                                                    aGAAAACGCATTTCTGACCGACGTTATAACGCCCGGTGTCACCTACTTCCTCGTTGACGACGATCTTACGAATATTCTTCg       <  1:805179/81‑1 (MQ=255)                                                       aaaCGCATTTCTGACCGACGTTATAACGCCCGGTGTCACCTACTTCCTCGTTGACGACGATCTTACGAATATTCTTCGccc    <  1:805181/81‑1 (MQ=255)                                                       aaaCGCATTTCTGACCGACGTTATAACGCCCGGTGTCACCTACTTCCTCGTTGACGACGATCTTACGAATATTCTTCGccc    <  2:805180/81‑1 (MQ=255)                                                        aaCGCATTTCTGACCGACGTTATAACGCCCGGTGTCACCTACTTCCTCGTTGACGACGATCTTACGAATATTCTTCGCCCa   <  2:805182/81‑1 (MQ=255)                                                         aCGCATTTCTGACCGACGTTATAACGCCCGGTGTCACCTACTTCCTCGTTGACGACGATCTTACGAATATTCTTCGCCCa   >  1:805219/1‑80 (MQ=255)                                                          cGCATTTCTGACCGACGTTATAACGCCCGGTGTCACCTACTTCCTCGTTGACGACGATCTTACGAATATTCTTCGCCCaa  >  2:805242/1‑80 (MQ=255)                                                          cGCATTTCTGACCGACGTTATAACGCCCGGTGTCACCTACTTCCTCGTTGACGACGATCTTACGAATATTCTTCGCCCaa  >  1:805236/1‑80 (MQ=255)                                                            cATTTCTGACCGACGTTATAACGCCCGGTGTCACCTACTTCCTCGTTGACGACGATCTTACGAATATTCTTCGCCCaa  >  1:805204/1‑78 (MQ=255)                                                             aTTTCTGACCGACGTTATAACGCCCGGTGTCACCTACTTCCTCGTTGACGACGATCTTACGAATATTCTTCGCCCaa  >  1:805207/1‑77 (MQ=255)                                                                         |                                                                 AGATAATCACGCCGCTCACCAGCAGCAGAACCAGGAAAATAATCGCCGCCCAGAAAACGCATTTCTGACCGAAGTTATAACGCCCGGTGTCACCTACTTCCTCGTTGACGACGATCTTACGAATATTCTTCGCCCAA  >  NC_000913/4081464‑4081600

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑