Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,104,429	C→T	A183T (GCC→ACC)	cpxA ←	sensory histidine kinase CpxA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,104,429	0	C	T	100.0%	157.2 / NA	43	A183T (GCC→ACC)	cpxA	sensory histidine kinase CpxA
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (22/21);  total (22/21)

CTGGCGTAAGTTTCCCTGGGCAACTTCATCGGCAGCGTTTTTCAGCTTACGCGCCGGTTTTGCCAGACTCCAGGCCAACCACAACAGCAGCGGCGTACTGACCAACATGGTGACAATCAGCAGTAATAGCGGGCGGTCAAACAGTAAGTTA  >  NC_000913/4104355‑4104505                                                                           |                                                                             cTGGCGTAAGTTTCCCTGGGCAACTTCATCGGCAGCGTTTTTCAGCTTACGCGCCGGTTTTGCCAGACTCCAGGTCAAcc                                                                         <  2:810695/80‑1 (MQ=255)    gCGTAAGTTTCCCTGGGCAACTTCATCGGCAGCGTTTTTCAGCTTACGCGCCGGTTTTGCCAGACTCCAGGTCAACCacaa                                                                     <  1:810696/81‑1 (MQ=255)     cGTAAGTTTCCCTGGGCAACTTCATCGGCAGCGTTTTTCAGCTTACGCGCCGGTTTTGCCAGACTCCAGGTCAACCacaac                                                                    <  2:810697/81‑1 (MQ=255)      gTAAGTTTCCCTGGGCAACTTCATCGGCAGCGTTTTTCAGCTTACGCGCCGGTTTTGCCAGACTCCAGGTCAACCacaa                                                                     >  1:810741/1‑79 (MQ=255)        aaGTTTCCCTGGGCAACTTCATCGGCAGCGTTTTTCAGCTTACGCGCCGGTTTTGCCAGACTCCAGGTCAACCACAAcag                                                                  >  2:810745/1‑80 (MQ=255)             tCCCTGGGCAACTTCATCGGCAGCGTTTTTCAGCTTACGCGCCGGTTTTGCCAGACTCCAGGTCAACCACAACAGCAgcgg                                                            >  2:810756/1‑81 (MQ=255)               ccTGGGCAACTTCATCGGCAGCGTTTTTCAGCTTACGCGCCGGTTTTGCCAGACTCCAGGTCAACCACAACAGCAgcggc                                                           <  1:810698/80‑1 (MQ=255)                cTGGGCAACTTCATCGGCAGCGTTTTTCAGCTTACGCGCCGGTTTTGCCAGACTCCAGGTCAACCACAACAGCAGCGGCGt                                                         <  2:810699/81‑1 (MQ=255)                  gggCAACTTCATCGGCAGCGTTTTTCAGCTTACGCGCCGGTTTTGCCAGACTCCAGGTCAACCACAACAGCAGCGGCGTAc                                                       >  1:810735/1‑81 (MQ=255)                   ggCAACTTCATCGGCAGCGTTTTTCAGCTTACGCGCCGGTTTTGCCAGACTCCAGGTCAACCACAACAGCAGCGGCGTa                                                        <  1:810701/79‑1 (MQ=255)                   ggCAACTTCATCGGCAGCGTTTTTCAGCTTACGCGCCGGTTTTGCCAGACTCCAGGTCAACCACAACAGCAGCGGCGTa                                                        <  1:810703/79‑1 (MQ=255)                   ggCAACTTCATCGGCAGCGTTTTTCAGCTTACGCGCCGGTTTTGCCAGACTCCAGGTCAACCACAACAGCAGCGGCGTa                                                        <  2:810700/79‑1 (MQ=255)                   ggCAACTTCATCGGCAGCGTTTTTCAGCTTACGCGCCGGTTTTGCCAGACTCCAGGTCAACCACAACAGCAGCGGCGTAc                                                       <  2:810702/80‑1 (MQ=255)                    gCAACTTCATCGGCAGCGTTTTTCAGCTTACGCGCCGGTTTTGCCAGACTCCAGGTCAACCACAACAGCAGCGGCGTACTg                                                     <  2:810704/81‑1 (MQ=255)                           cATCGGCAGCGTTTTTCAGCTTACGCGCCGGTTTTGCCAGACTCCAGGTCAACCACAACAGCAGCGGCGTACTGACCAACa                                              >  2:810754/1‑81 (MQ=255)                           cATCGGCAGCGTTTTTCAGCTTACGCGCCGGTTTTGCCAGACTCCAGGTCAACCACAACAGCAGCGGCGTACTGACCAACa                                              >  2:810749/1‑81 (MQ=255)                            aTCGGCAGCGTTTTTCAGCTTACGCGCCGGTTTTGCCAGACTCCAGGTCAACCACAACAGCAGCGGCGTACTGACCAACAt                                             >  1:810750/1‑81 (MQ=255)                              cGGCAGCGTTTTTCAGCTTACGCGCCGGTTTTGCCAGACTCCAGGTCAACCACAACAGCAGCGGCGTACTGACCAACATgg                                           >  1:810746/1‑81 (MQ=255)                               ggCAGCGTTTTTCAGCTTACGCGCCGGTTTTGCCAGACTCCAGGTCAACCACAACAGCAGCGGCGTACTGACCAACATGGt                                          >  2:810740/1‑81 (MQ=255)                               ggCAGCGTTTTTCAGCTTACGCGCCGGTTTTGCCAGACTCCAGGTCAACCACAACAGCAGCGGCGTACTGACCAACATGGt                                          <  1:810705/81‑1 (MQ=255)                                 cAGCGTTTTTCAGCTTACGCGCCGGTTTTGCCAGACTCCAGGTCAACCACAACAGCAGCGGCGTACTGACCAACATGGTGa                                        >  1:810731/1‑81 (MQ=255)                                  aGCGTTTTTCAGCTTACGCGCCGGTTTTGCCAGACTCCAGGTCAACCACAACAGCAGCGGCGTACTGACCAACATGGTGAc                                       <  2:810706/81‑1 (MQ=255)                                    cGTTTTTCAGCTTACGCGCCGGTTTTGCCAGACTCCAGGTCAACCACAACAGCAGCGGCGTACTGACCAACATGGTGACaa                                     >  2:810737/1‑81 (MQ=255)                                     gTTTTTCAGCTTACGCGCCGGTTTTGCCAGACTCCAGGTCAACCACAACAGCAGCGGCGTACTGACCAACATGGTGACAAt                                    >  2:810707/1‑81 (MQ=255)                                      tttttCAGCTTACGCGCCGGTTTTACCAGACTCCAGGTCAACCACAACAGCAGCGGCGTACTGACCAACATGGTGACAATc                                   <  2:810708/81‑1 (MQ=255)                                          tCAGCTTACGCGCCGGTTTTGCCAGACTCCAGGTCAACCACAACAGCAGCGGCGTACTGACCAACATGGTGACAATcagc                                <  1:810709/80‑1 (MQ=255)                                          tCAGCTTACGCGCCGGTTTTGCCAGACTCCAGGTCAACCACAACAGCAGCGGCGTACTGACCAACATGGTGACAATcag                                 >  2:810736/1‑79 (MQ=255)                                            aGCTTACGCGCCTGTTTTGCCAGACTCCAGGTCAACCACAACAGCAGCGGCGTACTGACCAACATGGTGACAATcagcag                              <  2:810710/80‑1 (MQ=255)                                             gCTTACGCGCCGGTTTTGCCAGACTCCAGGTCAACCACAACAGCAGCGGCGTACTGACCAACATGGTGACAATCAGCAGt                             >  1:810753/1‑80 (MQ=255)                                                tACGCGCCGGTTTTGCCAGACTCCAGGTCAACCACAACAGCAGCGGCGTACTGACCAACATGGTGACAATCAGCAGTaa                           >  1:810758/1‑79 (MQ=255)                                                 aCGCGCCGGTTTTGCCAGACTCCAGGTCAACCACAACAGCAGCGGCGTACTGACCAACATGGTGACAATCAGCAGTAATAg                        >  2:810767/1‑81 (MQ=255)                                                   gcgcCGGTTTTGCCAGACTCCAGGTCAACCACAACAGCAGCGGCGTACTGACCAACATGGTGACAATCAGCAGTAATAgcg                      <  1:810711/81‑1 (MQ=255)                                                     gcCGGTTTTGCCAGACTCCAGGTCAACCACAACAGCAGCGGCGTACTGACCAACATGGTGACAATCAGCAGTAATAgcggg                    >  1:810761/1‑81 (MQ=255)                                                      ccGGTTTTGCCAGACTCCAGGTCAACCACAACAGCAGCGGCGTACTGACCAACATGGTGACAATCAGCAGTAATAgcgggc                   <  1:810712/81‑1 (MQ=255)                                                        ggTTTTGCCAGACTCCAGGTCAACCACAACAGCAGCGGCGTACTGACCAACATGGTGACAATCAGCAGTAATAgcgggcgg                 >  2:810738/1‑81 (MQ=255)                                                            ttGCCAGACTCCAGGTCAACCACAACAGCAGCGGCGTACTGACCAACATGGTGACAATCAGCAGTAATAGCGGGCGGTCa              >  2:810751/1‑80 (MQ=255)                                                              gCCAGACTCCAGGTCAACCACAACAGCAGCAGCGTACTGACCAACATGGTGACAATCAGCAGTAATAGCGGGCGGTCaaa            <  2:810713/80‑1 (MQ=255)                                                               ccAGACTCCAGGTCAACCACAACAGCAGCGGCGTACTGACCAACATGGTGACAATCAGCAGTAATAGCGGGCGGTCAAAc           <  1:810714/80‑1 (MQ=255)                                                                cAGACTCCAGGTCAACCACAACAGCAGCGGCGTACTGACCAACATGGTGACAATCAGCAGTAATAGCGGGCGGTCAAACAg         >  2:810747/1‑81 (MQ=255)                                                                cAGACTCCAGGTCAACCACAACAGCAGCGGCGTACTGACCAACATGGTGACAATCAGCAGTAATAGCGGGCGGTCAAACAg         >  2:810748/1‑81 (MQ=255)                                                                      ccAGGTCAACCACAACAGCAGCGGCGTACTGACCAACATGGTGACAATCAGCAGTAATAGCGGGCGGTCAAACAGTAAGt    >  2:810764/1‑80 (MQ=255)                                                                       cAGGTCAACCACAACAGCAGCGGCGTACTGACCAACATGGTGACAATCAGCAGTAATAGCGGGCGGTCAAACAGTAAGtt   <  2:810716/80‑1 (MQ=255)                                                                       cAGGTCAACCACAACAGCAGCGGCGTACTGACCAACATGGTGACAATCAGCAGTAATAGCGGGCGGTCAAACAGTAAGTTa  <  2:810715/81‑1 (MQ=255)                                                                           |                                                                             CTGGCGTAAGTTTCCCTGGGCAACTTCATCGGCAGCGTTTTTCAGCTTACGCGCCGGTTTTGCCAGACTCCAGGCCAACCACAACAGCAGCGGCGTACTGACCAACATGGTGACAATCAGCAGTAATAGCGGGCGGTCAAACAGTAAGTTA  >  NC_000913/4104355‑4104505

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑