Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,183,748	A→G	E835G (GAA→GGA)	rpoB →	RNA polymerase subunit beta

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,183,748	0	A	G	100.0%	163.4 / NA	44	E835G (GAA→GGA)	rpoB	RNA polymerase subunit beta
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (24/20);  total (24/20)

ACAACTTCGAAGACTCCATCCTCGTATCCGAGCGTGTTGTTCAGGAAGACCGTTTCACCACCATCCACATTCAGGAACTGGCGTGTGTGTCCCGTGACACCAAGCTGGGTCCGGAAGAGATCACCGCTGACATCCCGAACGTGGGTGAAGCTG  >  NC_000913/4183673‑4183825                                                                            |                                                                              aCAACTTCGAAGACTCCATCCTCGTATCCGAGCGTGTTGTTCAGGAAGACCGTTTCACCACCATCCACATTCAGGGACTg                                                                           <  1:829862/80‑1 (MQ=255)      ttCGAAGACTCCATCCTCGTATCCGAGCGTGTTGTTCAGGAAGACCGTTTCACCACCATCCACATTCAGGGACTGGCgtgt                                                                     >  1:829910/1‑81 (MQ=255)        cGAAGACTCCATCCTCGTATCCGAGCGTGTTGTTCAGGAAGACCGTTTCACCACCATCCACATTCAGGGACTGGCgtgtgt                                                                   >  1:829918/1‑81 (MQ=255)        cGAAGACTCCATCCTCGTATCCGAGCGTGTTGTTCAGGAAGACCGTTTCACCACCATCCACATTCAGGGACTGGCgtgtgt                                                                   >  2:829931/1‑81 (MQ=255)         gAAGACTCCATCCTCGTATCCGAGCGTGTTGTTCAGGAAGACCGTTTCACCACCATCCACATTCAGGGACTGGCgtgtgtg                                                                  >  1:829913/1‑81 (MQ=255)             aCTCCATCCTCGTATCCGAGCGTGTTGTTCAGGAAGACCGTTTCACCACCATCCACATTCAGGGACTGGCGTGTGTGTccc                                                              <  2:829863/81‑1 (MQ=255)             aCTCCATCCTCGTATCCGAGCGTGTTGTTCAGGAAGACCGTTTCACCACCATCCACATTCAGGGACTGGCGTGTGTGTccc                                                              >  2:829933/1‑81 (MQ=255)                 cATCCTCGTATCCGAGCGTGTTGTTCAGGAAGACCGTTTCACCACCATCCACATTCAGGGACTGGCGTGTGTGTCCCGt                                                            <  2:829864/79‑1 (MQ=255)                 cATCCTCGTATCCGAGCGTGTTGTTCAGGAAGACCGTTTCACCACCATCCACATTCAGGGACTGGCGTGTGTGTCCCGTGa                                                          >  1:829950/1‑81 (MQ=255)                        gTATCCGAGCGTGTTGTTCAGGAAGACCGTTTCACCACCATCCACATTCAGGGACTGGCGTGTGTGTCCCGTGACACCAAg                                                   >  2:829934/1‑81 (MQ=255)                        gTATCCGAGCGTGTTGTTCAGGAAGACCGTTTCACCACCATCCACATTCAGGGACTGGCGTGTGTGTCCCGTGACACCAAg                                                   >  2:829944/1‑81 (MQ=255)                         tATCCGAGCGTGTTGTTCAGGAAGACCGTTTCACCACCATCCACATTCAGGGACTGGCGTGTGTGTCCCGTGACACCAAGc                                                  <  1:829865/81‑1 (MQ=255)                         tATCCGAGCGTGTTGTTCAGGAAGACCGTTTCACCACCATCCACATTCAGGGACTGGCGTGTGTGTCCCGTGACACCAAGc                                                  >  1:829920/1‑81 (MQ=255)                             cGAGCGTGTTGTTCAGGAAGACCGTTTCACCACCATCCACATTCAGGGACTGGCGTGTGTGTCCCGTGACACCAAGCTggg                                              <  2:829866/81‑1 (MQ=255)                              gAGCGTGTTGTTCAGGAAGACCGTTTCACCACCATCCACATTCAGGGACTGGCGTGTGTGTCCCGTGACACCAAGCTGGGt                                             >  1:829935/1‑81 (MQ=255)                                gCGTGTTGTTCAGGAAGACCGTTTCACCACCATCCACATTCAGGGACTGGCGTGTGTGTCCCGTGACACCAAGCTGGGTcc                                           >  2:829921/1‑81 (MQ=255)                                   tgttgtTCAGGAAGACCGTTTCACCACCATCCACATTCAGGGACTGGCGTGTGTGTCCCGTGACACCAAGCTGGGTCCGGa                                        <  1:829867/81‑1 (MQ=255)                                   tgttgtTCAGGAAGACCGTTTCACCACCATCCACATTCAGGGACTGGCGTGTGTGTCCCGTGACACCAAGCTGGGTCCGGa                                        <  1:829868/81‑1 (MQ=255)                                   tgttgtTCAGGAAGACCGTTTCACCACCATCCACATTCAGGGACTGGCGTGTGTGTCCCGTGACACCAAGCTGGGTCCGGa                                        <  1:829869/81‑1 (MQ=255)                                    gttgttCAGGAAGACCGTTTCACCACCATCCACATTCAGGGACTGGCGTGTGTGTCCCGTGACACCAAGCTGGGTCCgg                                         >  1:829938/1‑79 (MQ=255)                                       gttCAGGAAGACCGTTTCACCACCATCCACATTCAGGGACTGGCGTGTGTGTCCCGTGACACCAAGCTGGGTCCGGAaga                                     <  1:829870/80‑1 (MQ=255)                                        ttCAGGAAGACCGTTTCACCACCATCCACATTCAGGGACTGGCGTGTGTGTCCCGTGACACCAAGCTGGGTCCGGAagag                                    <  1:829871/80‑1 (MQ=255)                                         tCAGGAAGACCGTTTCACCACCATCCACATTCAGGGACTGGCGTGTGTGTCCCGTGACACCAAGCTGGGTCCGGaa                                       >  2:829927/1‑76 (MQ=255)                                           aGGAAGACCGTTTCACCACCATCCACATTCAGGGACTGGCGTGTGTGTCCCGTGACACCAAGCTGGGTCCGGAAGAGATc                                 <  2:829873/80‑1 (MQ=255)                                           aGGAAGACCGTTTCACCACCATCCACATTCAGGGACTGGCGTGTGTGTCCCGTGACACCAAGCTGGGTCCGGAAGAGATCa                                <  2:829872/81‑1 (MQ=255)                                           aGGAAGACCGTTTCACCACCATCCACATTCAGGGACTGGCGTGTGTGTCCCGTGACACCAAGCTGGGTCCGGAAGAGATCa                                >  1:829942/1‑81 (MQ=255)                                            ggAAGACCGTTTCACCACCATCCACATTCAGGGACTGGCGTGTGTGTCCCGTGACACCAAGCTGGGTCCGGAAGAGATCAc                               >  1:829941/1‑81 (MQ=255)                                             gAAGACCGTTTCACCACCATCCACATTCAGGGACTGGCGTGTGTGTCCCGTGACACCAAGCTGGGTCCGGAAGAGATCAcc                              >  2:829946/1‑81 (MQ=255)                                               aGACCGTTTCACCACCATCCACATTCAGGGACTGGCGTGTGTGTCCCGTGACACCAAGCTGGGTCCGGAAGAGATCACCGc                            >  2:829926/1‑81 (MQ=255)                                               aGACCGTTTCACCACCATCCACATTCAGGGACTGGCGTGTGTGTCCCGTGACACCAAGCTGGGTCCGGAAGAGATCACCGc                            <  1:829874/81‑1 (MQ=255)                                                   cGTTTCACCACCATCCACATTCAGGGACTGGCGTGTGTGTCCCGTGACACCAAGCTGGGTCCGGAAGAGATCACCGCTGAc                        <  2:829875/81‑1 (MQ=255)                                                   cGTTTCACCACCATCCACATTCAGGGACTGGCGTGTGTGTCCCGTGACACCAAGCTGGGTCCGGAAGAGATCACCGCTGAc                        <  1:829876/81‑1 (MQ=255)                                                       tCACCACCATCCACATTCAGGGACTGGCGTGTGTGTCCCGTGACACCAAGCTGGGTCCGGAAGAGATCACCGCTGACATcc                    >  2:829930/1‑81 (MQ=255)                                                        caccacCATCCACATTCAGGGACTGGCGTGTGTGTCCCGTGACACCAAGCTGGGTCCGGAAGAGATCACCGCTGACATcc                    <  1:829877/80‑1 (MQ=255)                                                         accaccATCCACATTCAGGGACTGGCGTGTGTGTCCCGTGACACCAAGCTGGGTCCGGAAGAGATCACCGCTGACATccc                   >  1:829948/1‑80 (MQ=255)                                                                tCCACATTCTGGGACTGGCGTGTGTGTCCCGTGACACCAAGCTGGGTCCGGAAGAGATCACCGCTGACATCCCGAACGTgg           >  2:829945/1‑81 (MQ=255)                                                                  cacaTTCAGGGACTGGCGTGTGTGTCCCGTGACACCAAGCTGGGTCCGGAAGAGATCACCGCTGACATCCCGAACGTggg          <  1:829878/80‑1 (MQ=255)                                                                      ttCAGGGACTGGCGTGTGTGTCCCGTGACACCAAGCTGGGTCCGGAAGAGATCACCGCTGACATCCCGAACGTGGGTGAAg     <  1:829879/81‑1 (MQ=255)                                                                       tCAGGGACTGGCGTGTGTGTCCCGTGACACCAAGCTGGGTCCGGAAGAGATCACCGCTGACATCCCGAACGTGGGTGAAGc    >  1:829912/1‑81 (MQ=255)                                                                        cAGGGACTGGCGTGTGTGTCCCGTGACACCAAGCTGGGTCCGGAAGAGATCACCGCTGACATCCCGAACGTGGGTGAAgat   >  2:829947/1‑79 (MQ=255)                                                                        cAGGGACTGGCGTGTGTGTCCCGTGACACCAAGCTGGGTCCGGAAGAGATCACCGCTGACATCCCGAACGTGGGTGAAGc    <  1:829880/80‑1 (MQ=255)                                                                         aGGGACTGGCGTGTGTGTCCCGTGACACCAAGCTGGGTCCGGAAGAGATCACCGCTGACATCCCGAACGTGGGTGAAGCt   <  2:829881/80‑1 (MQ=255)                                                                         aGGGACTGGCGTGTGTGTCCCGTGACACCAAGCTGGGTCCGGAAGAGATCACCGCTGACATCCCGAACGTGGGTGAAGCTg  >  2:829929/1‑81 (MQ=255)                                                                         aGGGACTGGCGTGTGTGTCCCGTGACACCAAGCTGGGTCCGGAAGAGATCACCGCTGACATCCCGAACGTGGGTGAAGCTg  >  1:829936/1‑81 (MQ=255)                                                                            |                                                                              ACAACTTCGAAGACTCCATCCTCGTATCCGAGCGTGTTGTTCAGGAAGACCGTTTCACCACCATCCACATTCAGGAACTGGCGTGTGTGTCCCGTGACACCAAGCTGGGTCCGGAAGAGATCACCGCTGACATCCCGAACGTGGGTGAAGCTG  >  NC_000913/4183673‑4183825

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑