Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,276,458	A→G	M330V (ATG→GTG)	pdeC →	c‑di‑GMP phosphodiesterase PdeC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,276,458	0	A	G	100.0%	82.2 / NA	23	M330V (ATG→GTG)	pdeC	c‑di‑GMP phosphodiesterase PdeC
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (14/9);  total (14/9)

GTTGTTACGTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTATGTTATTGATAATGTCTTCCGCGATCTGGGCGATTACCTGGCAACACATG  >  NC_000913/4276382‑4276530                                                                             |                                                                         gttgttACGTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGggtga                                                                       >  2:852049/1‑80 (MQ=255)  ttgttACGTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGGTGATa                                                                     <  2:852000/81‑1 (MQ=255)   tgttACGTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGggtga                                                                       >  1:852038/1‑78 (MQ=255)     ttACGTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGGTGATAGa                                                                   <  2:852001/80‑1 (MQ=255)         gTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGGTGATAGaa                                                                  >  1:852030/1‑77 (MQ=255)            ggCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGGTGATAGAACAGATa                                                            <  1:852003/80‑1 (MQ=255)            ggCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGGTGATAGAACAGATa                                                            <  2:852002/80‑1 (MQ=255)               cTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGGTGATAGAACAGATAACTg                                                        <  1:852004/81‑1 (MQ=255)                  gTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGGTGATAGAACAGATAACTGAt                                                      <  2:852005/80‑1 (MQ=255)                    gAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGGTGATAGAACAGATAACTGATTAt                                                   >  2:852044/1‑81 (MQ=255)                    gAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGGTGATAGAACAGATAACTGATTAt                                                   >  1:852046/1‑81 (MQ=255)                        aGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGGTGATAGAACAGATAACTGATTATGTTa                                               >  2:852055/1‑81 (MQ=255)                           ggCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGGTGATAGAACAGATAACTGATTATGTTAtt                                             >  2:852042/1‑80 (MQ=255)                                ataatGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGGTGATAGAACAGATAACTGATTATGTTATTGATAAt                                       <  1:852006/81‑1 (MQ=255)                                    tGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGGTGATAGAACAGATAACTGATTATGTTATTGATAATGTCt                                   >  1:852050/1‑81 (MQ=255)                                       aTCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGGTGATAGAACAGATAACTGATTATGTTATTGATAATGTCTTcc                                <  1:852007/81‑1 (MQ=255)                                          cGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGGTGATAGAACAGATAACTGATTATGTTATTGATAATGTCTTCcgc                              >  1:852045/1‑80 (MQ=255)                                           ggCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGGTGATAGAACAGATAACTGATTATGTTATTGATAATGTCTTCCGCGa                            >  1:852048/1‑81 (MQ=255)                                                     aTTCCGCTGGCAGAAAAGGAGGGGGTGATAGAACAGATAACTGATTATGTTATTGATAATGTCTTCCGCGATCTGGGCGAt                  >  2:852035/1‑81 (MQ=255)                                                             ggCAGAAAAGGAGGGGGTGATAGAACAGATAACTGATTATGTTATTGATAATGTCTTCCGCGATCTGGGCGATTACCTgg           >  2:852054/1‑80 (MQ=255)                                                              gCAGAAAAGGAGGGGGTGATAGAACAGATAACTGATTATGTTATTGATAATGTCTTCCGCGATCTGGGCGATTACCTgg           <  2:852008/79‑1 (MQ=255)                                                               cAGAAAAGGAGGGGGTGATAGAACAGATAACTGATTATGTTATTGATAATGTCTTCCGCGATCTGGGCGATTACCTGGCaa        >  1:852067/1‑81 (MQ=255)                                                                  aaaaGGAGGGGGTGATAGAACAGATAACTGATTATGTTATTGATAATGTCTTCCGCGATCTGGGCGATTACCTGGCAaca      >  1:852059/1‑80 (MQ=255)                                                                    aaGGAGGGGGTGATAGAACAGATAACTGATTATGTTATTGATAATGTCTTCCGCGATCTGGGCGATTACCTGGCAACACAt   >  1:852047/1‑81 (MQ=255)                                                                       gaggGGGTGATAGAACAGATAACTGATTATGTTATTGATAATGTCTTCCGCGATCTGGGCGATTACCTGGCAACACATg  >  2:852052/1‑79 (MQ=255)                                                                             |                                                                         GTTGTTACGTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTATGTTATTGATAATGTCTTCCGCGATCTGGGCGATTACCTGGCAACACATG  >  NC_000913/4276382‑4276530

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑