Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,319,653	G→A	F337F (TTC→TTT)	phnI ←	carbon‑phosphorus lyase core complex subunit PhnI

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,319,653	0	G	A	100.0%	197.6 / NA	54	F337F (TTC→TTT)	phnI	carbon‑phosphorus lyase core complex subunit PhnI
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (21/33);  total (21/33)

TAGGCAAAGTTGTAGCCGCTCAGATTAGCCATGGTTCTGCTCCTGTTGCAGACGTTTGAGTAGCTCCAGTTCGGCCTGGAAATCGACGTAGTGGGGGAGTTTGAGGTGTGAGACAAAGCCTGCGGCTTCGACGTTGTCGGCATGTGCCAGCACGAA  >  NC_000913/4319575‑4319730                                                                               |                                                                              tAGGCAAAGTTGTAGCCGCTCAGATTAGCCATGGTTCTGCTCCTGTTGCAGACGTTTGAGTAGCTCCAGTTCGGCCTGaaa                                                                             <  1:862597/81‑1 (MQ=255)    gCAAAGTTGTAGCCGCTCAGATTAGCCATGGTTCTGCTCCTGTTGCAGACGTTTGAGTAGCTCCAGTTCGGCCTGAAAATc                                                                          <  1:862598/81‑1 (MQ=255)     cAAAGTTGTAGCCGCTCAGATTAGCCATGGTTCTGCTCCTGTTGCAGACGTTTGAGTAGCTCCAGTTCGGCCTGAAAATc                                                                          >  1:862672/1‑80 (MQ=255)        aGTTGTAGCCGCTCAGATTAGCCATGGTTCTGCTCCTGTTGCAGACGTTTGAGTAGCTCCAGTTCGGCCTGAAAATCGACg                                                                      >  2:862657/1‑81 (MQ=255)             tAGCCGCTCAGATTAGCCATGGTTCTGCTCCTGTTGCAGACGTTTGAGTAGCTCCAGTTCGGCCTGAAAATCGACGTAGTg                                                                 <  2:862599/81‑1 (MQ=255)               gCCGCTCAGATTAGCCATGGTTCTGCTCCTGTTGCAGACGTTTGAGTAGCTCCAGTTCGGCCTGAAAATCGACGTAGTgg                                                                <  1:862600/80‑1 (MQ=255)                 cGCTCAGATTAGCCATGGTTCTGCTCCTGTTGCAGACGTTTGAGTAGCTCCAGTTCGGCCTGAAAATCGACGTAGTggggg                                                             <  2:862601/81‑1 (MQ=255)                  gCTCAGATTAGCCATGGTTCTGCTCCTGTTGCAGACGTTTGAGTAGCTCCAGTTCGGCCTGAAAATCGACGTAGTgggg                                                              >  2:862674/1‑79 (MQ=255)                  gCTCAGATTAGCCATGGTTCTGCTCCTGTTGCAGACGTTTGAGTAGCTCCAGTTCGGCCTGAAAATCGACGTAGTGGGGGa                                                            <  2:862602/81‑1 (MQ=255)                      aGATTAGCCATGGTTCTGCTCCTGTTGCAGACGTTTGAGTAGCTCCAGTTCGGCCTGAAAATCGACGTAGTGGGGGAGttt                                                        <  2:862605/81‑1 (MQ=255)                      aGATTAGCCATGGTTCTGCTCCTGTTGCAGACGTTTGAGTAGCTCCAGTTCGGCCTGAAAATCGACGTAGTGGGGGAGttt                                                        <  2:862604/81‑1 (MQ=255)                      aGATTAGCCATGGTTCTGCTCCTGTTGCAGACGTTTGAGTAGCTCCAGTTCGGCCTGAAAATCGACGTAGTGGGGGAGtt                                                         <  1:862603/80‑1 (MQ=255)                       gATTAGCCATGGTTCTGCTCCTGTTGCAGACGTTTGAGTAGCTCCAGTTCGGCCTGAAAATCGACGTAGTGGGGGAGttt                                                        >  1:862652/1‑80 (MQ=255)                       gATTAGCCATGGTTCTGCTCCTGTTGCAGACGTTTGAGTAGCTCCAGTTCGGCCTGAAAATCGACGTAGTGGGGGAGTTTg                                                       <  2:862606/81‑1 (MQ=255)                        aTTAGCCATGGTTCTGCTCCTGTTGCAGACGTTTGAGTAGCTCCAGTTCGGCCTGAAAATCGACGTAGTGGGGGAGttt                                                        >  1:862700/1‑79 (MQ=255)                         ttAGCCATGGTTCTGCTCCTGTTGCAGACGTTTGAGTAGCTCCAGTTCGGCCTGAAAATCGACGTAGTGGGGGAGTTTGAg                                                     >  1:862685/1‑81 (MQ=255)                           aGCCATGGTTCTGCTCCTGTTGCAGACGTTTGAGTAGCTCCAGTTCGGCCTGAAAATCGACGTAGTGGGGGAGTTTGAGgt                                                   >  2:862675/1‑81 (MQ=255)                           aGCCATGGTTCTGCTCCTGTTGCAGACGTTTGAGTAGCTCCAGTTCGGCCTGAAAATCGACGTAGTGGGGGAGTTTGAGgt                                                   >  2:862665/1‑81 (MQ=255)                            gCCATGGTTCTGCTCCTGTTGCAGACGTTTGAGTAGCTCCAGTTCGGCCTGAAAATCGACGTAGTGGGGGAGTTTGAGgtg                                                  >  2:862682/1‑81 (MQ=255)                            gCCATGGTTCTGCTCCTGTTGCAGACGTTTGAGTAGCTCCAGTTCGGCCTGAAAATCGACGTAGTGGGGGAGTTTGAGgtg                                                  >  2:862651/1‑81 (MQ=255)                            gCCATGGTTCTGCTCCTGTTGCAGACGTTTGAGTAGCTCCAGTTCGGCCTGAAAATCGACGTAGTGGGGGAGTTTGAGgtg                                                  <  1:862608/81‑1 (MQ=255)                            gCCATGGTTCTGCTCCTGTTGCAGACGTTTGAGTAGCTCCAGTTCGGCCTGAAAATCGACGTAGTGGGGGAGTTTGAGgt                                                   <  1:862607/80‑1 (MQ=255)                            gCCATGGTTCTGCTCCTGTTGCAGACGTTTGAGTAGCTCCAGTTCGGCCTGAAAATCGACGTAGTGGGGGAGTTTGAGgt                                                   <  1:862609/80‑1 (MQ=255)                              cATGGTTCTGCTCCTGTTGCAGACGTTTGAGTAGCTCCAGTTCGGCCTGAAAATCGACGTAGTGGGGGAGTTTGAGgtgt                                                 >  1:862668/1‑80 (MQ=255)                                tGGTTCTGCTCCTGTTGCAGACGTTTGAGTAGCTCCAGTTCGGCCTGAAAATCGACGTAGTGGGGGAGTTTGAGGTGTgag                                              <  1:862610/81‑1 (MQ=255)                                tGGTTCTGCTCCTGTTGCAGACGTTTGAGTAGCTCCAGTTCGGCCTGAAAATCGACGTAGTGGGGGAGTTTGAGGTGTgag                                              <  1:862611/81‑1 (MQ=255)                                      tGCTCCTGTTGCAGACGTTTGAGTAGCTCCAGTTCGGCCTGAAAATCGACGTAGTGGGGGAGTTTGAGGTGTGAGACAAAg                                        >  2:862677/1‑81 (MQ=255)                                        cTCCTGTTGCAGACGTTTGAGTAGCTCCAGTTCGGCCTGAAAATCGACGTAGTGGGGGAGTTTGAGGTGTGAGACAAAGcc                                      <  2:862612/81‑1 (MQ=255)                                        cTCCTGTTGCAGACGTTTGAGTAGCTCCAGTTCGGCCTGAAAATCGACGTAGTGGGGGAGTTTGAGGTGTGAGACAAAGc                                       <  2:862613/80‑1 (MQ=255)                                           cTGTTGCAGACGTTTGAGTAGCTCCAGTTCGGCCTGAAAATCGACGTAGTGGGGGAGTTTGAGGTGTGAGACAAAGCCt                                     <  1:862614/79‑1 (MQ=255)                                           cTGTTGCAGACGTTTGAGTAGCTCCAGTTCGGCCTGAAAATCGACGTAGTGGGGGAGTTTGAGGTGTGAGACAAAGCCTg                                    <  2:862616/80‑1 (MQ=255)                                           cTGTTGCAGACGTTTGAGTAGCTCCAGTTCGGCCTGAAAATCGACGTAGTGGGGGAGTTTGAGGTGTGAGACAAAGCCTGc                                   <  1:862617/81‑1 (MQ=255)                                           cTGTTGCAGACGTTTGAGTAGCTCCAGTTCGGCCTGAAAATCGACGTAGTGGGGGAGTTTGAGGTGTGAGACAAAGCCTGc                                   <  1:862615/81‑1 (MQ=255)                                             gTTGCAGACGTTTGAGTAGCTCCAGTTCGGCCTGAAAATCGACGTAGTGGGGGAGTTTGAGGTGTGAGACAAAGCCTGCgg                                 >  1:862664/1‑81 (MQ=255)                                              ttGCAGACGTTTGAGTAGCTCCAGTTCGGCCTGAAAATCGACGTAGTGGGGGAGTTTGAGGTGTGAGACAAAGCCTGCgg                                 <  1:862619/80‑1 (MQ=255)                                              ttGCAGACGTTTGAGTAGCTCCAGTTCGGCCTGAAAATCGACGTAGTGGGGGAGTTTGAGGTGTGAGACAAAGCCTGCg                                  <  2:862618/79‑1 (MQ=255)                                              ttGCAGACGTTTGAGTAGCTCCAGTTCGGCCTGAAAATCGACGTAGTGGGGGAGTTTGAGGTGTGAGACAAAGCCTGCGGc                                >  2:862666/1‑81 (MQ=255)                                               tGCAGACGTTTGAGTAGCTCCAGTTCGGCCTGAAAATCGACGTAGTGGGGGAGTTTGAGGTGTGAGACAAAGCCTGCGGCt                               >  2:862684/1‑81 (MQ=255)                                                gCAGACGTTTGAGTAGCTCCAGTTCGGCCTGAAAATCGACGTAGTGGGGGAGTTTGAGGTGTGAGACAAAGCCTGCGGCt                               <  1:862620/80‑1 (MQ=255)                                                 cAGACGTTTGAGTAGCTCCAGTTCGGCCTGAAAATCGACGTAGTGGGGGAGTTTGAGGTGTGAGACAAAGCCTGCGGCTTc                             <  1:862621/81‑1 (MQ=255)                                                    aCGTTTGAGTAGCTCCAGTTCGGCCTGAAAATCGACGTAGTGGGGGAGTTTGAGGTGTGAGACAAAGCCTGCGGCTTCGAc                          <  2:862622/81‑1 (MQ=255)                                                       tttGAGTAGCTCCAGTTCGGCCTGAAAATCGACGTAGTGGGGGAGTTTGAGGTGTGAGACAAAGCCTGCGGCTTCGACGtt                       <  1:862623/81‑1 (MQ=255)                                                       tttGAGTAGCTCCAGTTCGGCCTGAAAATCGACGTAGTGGGGGAGTTTGAGGTGTGAGACAAAGCCTGCGGCTTCGACGtt                       <  2:862624/81‑1 (MQ=255)                                                           aGTAGCTCCAGTTCGGCCTGAAAATCGACGTAGTGGGGGAGTTTGAGGTGTGAGACAAAGCCTGCGGCTTCGACGTTGTc                    <  1:862626/80‑1 (MQ=255)                                                           aGTAGCTCCAGTTCGGCCTGAAAATCGACGTAGTGGGGGAGTTTGAGGTGTGAGACAAAGCCTGCGGCTTCGACGTTGTCg                   <  1:862625/81‑1 (MQ=255)                                                                   cAGTTCGGCCTGAAAATCGACGTAGTGGGGGAGTTTGAGGTGTGAGACAAAGCCTGCGGCTTCGACGTTGTCGGCAtgt             <  1:862627/79‑1 (MQ=255)                                                                   cAGTTCGGCCTGAAAATCGACGTAGTGGGGGAGTTTGAGGTGTGAGACAAAGCCTGCGGCTTCGACGTTGTCGGCATGTGc           >  2:862687/1‑81 (MQ=255)                                                                     gTTCGGCCTGAAAATCGACGTAGTGGGGGAGTTTGAGGTGTGAGACAAAGCCTGCGGCTTCGACGTTGTCGGCATGTGc           >  1:862683/1‑79 (MQ=255)                                                                     gTTCGGCCTGAAAATCGACGTAGTGGGGGAGTTTGAGGTGTGAGACAAAGCCTGCGGCTTCGACGTTGTCGGCATGTGc           >  1:862680/1‑79 (MQ=255)                                                                         ggCCTGAAAATCGACGTAGTGGGGGAGTTTGAGGTGTGAGACAAAGCCTGCGGCTTCGACGTTGTCGGCATGTGCCAGCAc     >  1:862694/1‑81 (MQ=255)                                                                         ggCCTGAAAATCGACGTAGTGGGGGAGTTTGAGGTGTGAGACAAAGCCTGCGGCTTCGACGTTGTCGGCATGTGCCAGCAc     >  1:862663/1‑81 (MQ=255)                                                                          gCCTGAAAATCGACGTAGTGGGGGAGTTTGAGGTGTGAGACAAAGCCTGCGGCTTCGACGTTGTCGGCATGTGCCAGCACg    <  2:862630/81‑1 (MQ=255)                                                                          gCCTGAAAATCGACGTAGTGGGGGAGTTTGAGGTGTGAGACAAAGCCTGCGGCTTCGACGTTGTCGGCATGTGCCAGCACg    <  2:862629/81‑1 (MQ=255)                                                                          gCCTGAAAATCGACGTAGTGGGGGAGTTTGAGGTGTGAGACAAAGCCTGCGGCTTCGACGTTGTCGGCATGTGCCAGCACg    <  1:862628/81‑1 (MQ=255)                                                                            cTGAAAATCGACGTAGTGGGGGAGTTTGAGGTGTGAGACAAAGCCTGCGGCTTCGACGTTGTCGGCATGTGCCAGCACGaa  >  1:862673/1‑81 (MQ=255)                                                                               |                                                                              TAGGCAAAGTTGTAGCCGCTCAGATTAGCCATGGTTCTGCTCCTGTTGCAGACGTTTGAGTAGCTCCAGTTCGGCCTGGAAATCGACGTAGTGGGGGAGTTTGAGGTGTGAGACAAAGCCTGCGGCTTCGACGTTGTCGGCATGTGCCAGCACGAA  >  NC_000913/4319575‑4319730

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑