Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,323,424	G→T	T310N (ACC→AAC)	phnD ←	phosphonate ABC transporter periplasmic binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,323,424	0	G	T	100.0%	113.2 / NA	31	T310N (ACC→AAC)	phnD	phosphonate ABC transporter periplasmic binding protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (13/18);  total (13/18)

TTTACTCACCGAACTCATCGCGCTTAGCGCGTTGTTCAGGCGGTCCAGGTCATCCAGCTGCGCCTGAATCTCGGTGGTTTTTGCCAGCTTGTCCTGCTCATTCAGTCCTTTATTGCTTTTCACGCCCTGCATCTCTTTAAACAGCGCGAG  >  NC_000913/4323348‑4323497                                                                             |                                                                          tttACTCACCGAACTCATCGCGCTTAGCGCGTTGTTCAGGCGGTCCAGGTCATCCAGCTGCGCCTGAATCTCGGTGTtttt                                                                       >  2:863836/1‑81 (MQ=255)    aCTCACCGAACTCATCGCGCTTAGCGCGTTGTTCAGGCGGTCCAGGTCATCCAGCTGCGCCTGAATCTCGGTGTTTTTTg                                                                     <  2:863780/80‑1 (MQ=255)     cTCACCGAACTCATCGCGCTTAGCGCGTTGTTCAGGCGGTCCAGGTCATCCAGCTGCGCCTGAATCTCGGTGTTTTTTGc                                                                    <  1:863781/80‑1 (MQ=255)          cGAACTCATCGCGCTTAGCGCGTTGTTCAGGCGGTCCAGGTCATCCAGCTGCGCCTGAATCTCGGTGTTTTTTGCCAGCtt                                                              <  2:863782/81‑1 (MQ=255)            aaCTCATCGCGCTTAGCGCGTTGTTCAGGCGGTCCAGGTCATCCAGCTGCGCCTGAATCTCGGTGTTTTTTGCCAGCTTGt                                                            <  1:863783/81‑1 (MQ=255)               tCATCGCGCTTAGCGCGTTGTTCAGGCGGTCCAGGTCATCCAGCTGCGCCTGAATCTCGGTGTTTTTTGCCAGCTTGTCCt                                                         >  1:863833/1‑81 (MQ=255)                 aTCGCGCTTAGCGCGTTGTTCAGGCGGTCCAGGTCATCCAGCTGCGCCTGAATCTCGGTGTTTTTTGCCAGCTTGTCCt                                                         <  1:863784/79‑1 (MQ=255)                     cgcTTAGCGCGTTGTTCAGGCGGTCCAGGTCATCCAGCTGCGCCTGAATCTCGGTGTTTTTTGCCAGCTTGTCCTGCTCAt                                                   <  2:863785/81‑1 (MQ=255)                      gcTTAGCGCGTTGTTCAGGCGGTCCAGGTCATCCAGCTGCGCCTGAATCTCGGTGTTTTTTGCCAGCTTGTCCTGCTCAtt                                                  <  2:863786/81‑1 (MQ=255)                      gcTTAGCGCGTTGTTCAGGCGGTCCAGGTCATCCAGCTGCGCCTGAATCTCGGTGTTTTTTGCCAGCTTGTCCTGCTCAtt                                                  >  1:863825/1‑81 (MQ=255)                          aGCGCGTTGTTCAGGCGGTCCAGGTCATCCAGCTGCGCCTGAATCTCGGTGTTTTTTGCCAGCTTGTCCTGCTCATTCa                                                <  1:863787/79‑1 (MQ=255)                             gcgTTGTTCAGGCGGTCCAGGTCATCCAGCTGCGCCTGAATCTCGGTGTTTTTTGCCAGCTTGTCCTGCTCATTCAGTCCt                                           >  1:863834/1‑81 (MQ=255)                             gcgTTGTTCAGGCGGTCCAGGTCATCCAGCTGCGCCTGAATCTCGGTGTTTTTTGCCAGCTTGTCCTGCTCATTCAGTCCt                                           >  1:863843/1‑81 (MQ=255)                               gttgttCAGGCGGTCCAGGTCATCCAGCTGCGCCTGAATCTCGGTGTTTTTTGCCAGCTTGTCCTGCTCATTCAGTCCttt                                         <  1:863788/81‑1 (MQ=255)                               gttgttCAGGCGGTCCAGGTCATCCAGCTGCGCCTGAATCTCGGTGTTTTTTGCCAGCTTGTCCTGCTCATTCAGTCCtt                                          >  1:863824/1‑80 (MQ=255)                               gttgttCAGGCGGTCCAGGTCATCCAGCTGCGCCTGAATCTCGGTGTTTTTTGCCAGCTTGTCCTGCTCATTCAGTCCtt                                          >  1:863829/1‑80 (MQ=255)                               gttgttCAGGCGGTCCAGGTCATCCAGCTGCGCCTGAATCTCGGTGTTTTTTGCCAGCTTGTCCTGCTCATTCAGTCCtt                                          >  1:863832/1‑80 (MQ=255)                                      aGGCGGTCCAGGTCATCCAGCTGCGCCTGAATCTCGGTGTTTTTTGCCAGCTTGTCCTGCTCATTCAGTCCTTTATTGCtt                                  <  2:863789/81‑1 (MQ=255)                                        gCGGTCCAGGTCATCCAGCTGCGCCTGAATCTCGGTGTTTTTTGCCAGCTTGTCCTGCTCATTCAGTCCTTTATTGCtttt                                >  1:863835/1‑81 (MQ=255)                                         cGGTCCAGGTCATCCAGCTGCGCCTGAATCTCGGTGTTTTTTGCCAGCTTGTCCTGCTCATTCAGTCCTTTATTGCTTTTc                               <  2:863790/81‑1 (MQ=255)                                               aGGTCATCCAGCTGCGCCTGAATCTCGGTGTTTTTTGCCAGCTTGTCCTGCTCATTCAGTCCTTTATTGCTTTTCACGccc                         >  1:863838/1‑81 (MQ=255)                                               aGGTCATCCAGCTGCGCCTGAATCTCGGTGTTTTTTGCCAGCTTGTCCTGCTCATTCAGTCCTTTATTGCTTTTCACGccc                         >  2:863837/1‑81 (MQ=255)                                                   cATCCAGCTGCGCCTGAATCTCGGTGTTTTTTGCCAGCTTGTCCTGCTCATTCAGTCCTTTATTGCCTTTCACGCCCTGCa                     >  1:863840/1‑81 (MQ=255)                                                     tCCAGCTGCGCCTGAATCTCGGTGTTTTTTGCCAGCTTGTCCTGCTCATTCAGTCCTTTATTGCTTTTCACGCCCTGCAtc                   <  2:863791/81‑1 (MQ=255)                                                       cAGCTGCGCCTGAATCTCGGTGTTTTTTGCCAGCTTGTCCTGCTCATTCAGTCCTTTATTGCTTTTCACGCCCTGCAtct                  <  1:863792/80‑1 (MQ=255)                                                        aGCTGCGCCTGAATCTCGGTGTTTTTTGCCAGCTTGTCCTGCTCATTCAGTCCTTTATTGCTTTTCACGCCCTGCATctct                <  1:863793/81‑1 (MQ=255)                                                          cTGCGCCTGAATCTCGGTGTTTTTTGCCAGCTTGTCCTGCTCATTCAGTCCTTTATTGCTTTTCACGCCCTGCATCTCttt              <  1:863794/81‑1 (MQ=255)                                                              gcCTGAATCTCGGTGTTTTTTGCCAGCTTGTCCTGCTCATTCAGTCCTTTATTGCTTTTCACGCCCTGCATCTCTTTaaa           >  2:863847/1‑80 (MQ=255)                                                                   aaTCTCGGTGTTTTTTGCCAGCTTGTCCTGCTCATTCAGTCCTTTATTGCTTTTCACGCCCTGCATCTCTTTAAACAgcgc     <  2:863796/81‑1 (MQ=255)                                                                   aaTCTCGGTGTTTTTTGCCAGCTTGTCCTGCTCATTCAGTCCTTTATTGCTTTTCACGCCCTGCATCTCTTTAAACAgc       <  1:863795/79‑1 (MQ=255)                                                                    aTCTCGGTGTTTTTTGCCAGCTTGTCCTGCTCATTCAGTCCTTTATTGCTTTTCACGCCCTGCATCTCTTTAAACAGcgcg    <  1:863797/81‑1 (MQ=255)                                                                         ggtgttTTTTGCCAGCTTGTCCTGCTCATTCAGTCCTTTATTGCTTTTCACGCCCTGCATCTCTTTAAACAGCGCGAg  <  2:863798/78‑1 (MQ=255)                                                                             |                                                                          TTTACTCACCGAACTCATCGCGCTTAGCGCGTTGTTCAGGCGGTCCAGGTCATCCAGCTGCGCCTGAATCTCGGTGGTTTTTGCCAGCTTGTCCTGCTCATTCAGTCCTTTATTGCTTTTCACGCCCTGCATCTCTTTAAACAGCGCGAG  >  NC_000913/4323348‑4323497

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑