Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,333,287	T→C	N222D (AAC→GAC)	basR ←	DNA‑binding transcriptional dual regulator BasR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,333,287	0	T	C	100.0%	120.2 / NA	33	N222D (AAC→GAC)	basR	DNA‑binding transcriptional dual regulator BasR
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (16/17);  total (16/17)

AAATGGCCCCGATGGTCAATATCAGCCGTTGGCGCAGCGATATTGGTCGGCGCAGAAAATGCATCAGATTCAATTAGTTTTCCTCATTCGCGACCAGCATATAGCCAAAGCCGCGCACGGTGCGGATACGGGCTTTGCCCACTTTGTCGCG  >  NC_000913/4333209‑4333359                                                                               |                                                                         aaaTGGCCCCGATGGTCAATATCAGCCGTTGGCGCAGCGATATTGGTCGGCGCAGAAAATGCATCAGATTCAATTAGtctt                                                                        >  1:866179/1‑81 (MQ=255) aaaTGGCCCCGATGGTCAATATCAGCCGTTGGCGCAGCGATATTGGTCGGCGCAGAAAATGCATCAGATTCAATTAGtctt                                                                        <  2:866128/81‑1 (MQ=255) aaaTGGCCCCGATGGTCAATATCAGCCGTTGGCGCAGCGATATTGGTCGGCGCAGAAAATGCATCAGATTCAATTAGtctt                                                                        >  2:866164/1‑81 (MQ=255) aaaTGGCCCCGATGGTCAATATCAGCCGTTGGCGCAGCGATATTGGTCGGCGCAGAAAATGCATCAGATTCAATTAGtctt                                                                        >  1:866159/1‑81 (MQ=255)    tGGCCCCGATGGTCAATATCAGCCGTTGGCGCAGCGATATTGGTCGGCGCAGAAAATGCATCAGATTCAATTAGTCTTCCt                                                                     >  1:866193/1‑81 (MQ=255)       ccccGATGGTCAATATCAGCCGTTGGCGCAGCGATATTGGTCGGCGCAGAAAATGCATCAGATTCAATTAGTCTTCCTCAt                                                                  <  1:866129/81‑1 (MQ=255)         ccGATGGTCAATATCAGCCGTTGGCGCAGCGATATTGGTCGGCGCAGAAAATGCATCAGATTCAATTAGTCTTCCTCATTc                                                                <  2:866130/81‑1 (MQ=255)         ccGATGGTCAATATCAGCCGTTGGCGCAGCGATATTGGTCGGCGCAGAAAATGCATCAGATCCAATTAGTCTTCCTCATTc                                                                <  2:866131/81‑1 (MQ=255)           gATGGTCAATATCAGCCGTTGGCGCAGCGATATTGGTCGGCGCAGAAAATGCATCAGATTCAATTAGTCTTCCTCATTcgc                                                              >  1:866166/1‑81 (MQ=255)            aTGGTCAATATCAGCCGTTGGCGCAGCGATATTGGTCGGCGCAGAAAATGCATCAGATTCAATTAGTCTTCCTCATTcgcg                                                             <  2:866132/81‑1 (MQ=255)                 cAATATCAGCCGTTGGCGCAGCGATATTGGTCGGCGCAGAAAATGCATCAGATTCAATTAGTCTTCCTCATTCGCGACCAg                                                        >  1:866187/1‑81 (MQ=255)                    tatCAGCCGTTGGCGCAGCGATATTGGTCGGCGCAGAAAATGCATCAGATTCAATTAGTCTTCCTCATTCGCGACCAGCat                                                     <  1:866133/81‑1 (MQ=255)                    tatCAGCCGTTGGCGCAGCGATATTGGTCGGCGCAGAAAATGCATCAGATTCAATTAGTCTTCCTCATTCGCGACCAGCat                                                     <  2:866134/81‑1 (MQ=255)                       cAGCCGTTGGCGCAGCGATATTGGTCGGCGCAGAAAATGCATCAGATTCAATTAGTCTTCCTCATTCGCGACCAGCata                                                    >  1:866175/1‑79 (MQ=255)                        aGCCGTTGGCGCAGCGATATTGGTCGGCGCAGAAAATGCATCAGATTCAATTAGTCTTCCTCATTCGCGACCAGCATATAg                                                 >  2:866168/1‑81 (MQ=255)                             ttGGCGCAGCGATATTGGTCGGCGCAGAAAATGCATCAGATTCAATTAGTCTTCCTCATTCGCGACCAGCATATAGCCaaa                                            <  1:866135/81‑1 (MQ=255)                              tGGCGCAGCGATATTGGTCGGCGCAGAAAATGCATCAGATTCAATTAGTCTTCCTCATTCGCGACCAGCATATAGCCAAAg                                           >  1:866185/1‑81 (MQ=255)                                gcgcAGCGATATTGGTCGGCGCAGAAAATGCATCAGATTCAATTAGTCTTCCTCATTCGCGACCAGCATATAGCCAAAGcc                                         <  1:866136/81‑1 (MQ=255)                                  gcAGCGATATTGGTCGGCGCAGAAAATGCATCAGATTCAATTAGTCTTCCTCATTCGCGACCAGCATATAGCCAAAGCcgc                                       <  2:866137/81‑1 (MQ=255)                                    aGCGATATTGGTCGGCGCAGAAAATGCATCAGATTCAATTAGTCTTCCTCATTCGCGACCAGCATATAGCCAAAGCcgcg                                      >  1:866186/1‑80 (MQ=255)                                          atTGGTCGGCGCAGAAAATGCATCAGATTCAATTAGTCTTCCTCATTCGCGACCAGCATATAGCCAAAGCCGCGCACGGTg                               >  2:866189/1‑81 (MQ=255)                                          atTGGTCGGCGCAGAAAATGCATCAGATTCAATTAGTCTTCCTCATTCGCGACCAGCATATAGCCAAAGCCGCGCACGGTg                               >  1:866172/1‑81 (MQ=255)                                           ttGGTCGGCGCAGAAAATGCATCAGATTCAATTAGTCTTCCTCATTCGCGACCAGCATATAGCCAAAGCCGCGCACGGTGc                              >  1:866181/1‑81 (MQ=255)                                            tGGTCGGCGCAGAAAATGCATCAGATTCAATTAGTCTTCCTCATTCGCGACCAGCATATAGCCAAAGCCGCGCACGGTGCg                             >  1:866177/1‑81 (MQ=255)                                              gTCGGCGCAGAAAATGCATCAGATTCAATTAGTCTTCCTCATTCGCGACCAGCATATAGCCAAAGCCGCGCACGGTGCgg                            <  2:866138/80‑1 (MQ=255)                                              gTCGGCGCAGAAAATGCATCAGATTCAATTAGTCTTCCTCATTCGCGACCAGCATATAGCCAAAGCCGCGCACGGTGCGGa                           >  1:866167/1‑81 (MQ=255)                                              gTCGGCGCAGAAAATGCATCAGATTCAATTAGTCTTCCTCATTCGCGACCAGCATATAGCCAAAGCCGCGCACGGTGCGGa                           <  1:866139/81‑1 (MQ=255)                                                   cgcAGAAAATGCATCAGATTCAATTAGTCTTCCTCATTCGCGACCAGCATATAGCCAAAGCCGCGCACGGTGCGGATACgg                      <  1:866140/81‑1 (MQ=255)                                                   cgcAGAAAATGCATCAGATTCAATTAGTCTTCCTCATTCGCGACCAGCATATAGCCAAAGCCGCGCACGGTGCGGATACgg                      >  2:866171/1‑81 (MQ=255)                                                       gAAAATGCATCAGATTCAATTAGTCTTCCTCATTCGCGACCAGCATATAGCCAAAGCCGCGCACGGTGCGGATACGGGCtt                  <  1:866141/81‑1 (MQ=255)                                                            tGCATCAGATTCAATTAGTCTTCCTCATTCGCGACCAGCATATAGCCAAAGCCGCGCACGGTGCGGATACGGGCTTTGccc             <  1:866143/81‑1 (MQ=255)                                                            tGCATCAGATTCAATTAGTCTTCCTCATTCGCGACCAGCATATAGCCAAAGCCGCGCACGGTGCGGATACGGGCTTTGccc             <  1:866142/81‑1 (MQ=255)                                                                tCAGATTCAATTAGTCTTCCTCATTCGCGACCAGCATATAGCCAAAGCCGCGCACGGTTCGGATACGGGCTTTGCCCACtt         >  1:866174/1‑81 (MQ=255)                                                                  aGATTCAATTAGTCTTCCTCATTCGCGACCAGCATATAGCCAAAGCCGCGCACGGTGCGGATACGGGCTTTGCCCACttt        <  2:866144/80‑1 (MQ=255)                                                                   gATTCAATTAGTCTTCCTCATTCGCGACCAGCATATAGCCAAAGCCGCGCACGGTGCGGATACGGGCTTTGCCCACTTTGt      >  1:866176/1‑81 (MQ=255)                                                                    aTTCAATTAGTCTTCCTCATTCGCGACCAGCATATAGCCAAAGCCGCGCACGGTGCGGATACGGGCTTTGCCCACTTTGTc     <  2:866145/81‑1 (MQ=255)                                                                         aTTAGTCTTCCTCATTCGCGACCAGCATATAGCCAAAGCCGCGCACGGTGCGGATACGGGCTTTGCCCACTTTGTcgcg  >  2:866173/1‑79 (MQ=255)                                                                               |                                                                         AAATGGCCCCGATGGTCAATATCAGCCGTTGGCGCAGCGATATTGGTCGGCGCAGAAAATGCATCAGATTCAATTAGTTTTCCTCATTCGCGACCAGCATATAGCCAAAGCCGCGCACGGTGCGGATACGGGCTTTGCCCACTTTGTCGCG  >  NC_000913/4333209‑4333359

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑