Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,391,914	T→C	I104T (ATT→ACT)	orn →	oligoribonuclease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,391,914	0	T	C	100.0%	135.8 / NA	37	I104T (ATT→ACT)	orn	oligoribonuclease
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (18/19);  total (18/19)

GATGGGCGATCGGGAAGCTGAACTGGCAACGCTCGAATTTTTAAAACAGTGGGTGCCTGCGGGAAAATCGCCGATTTGCGGTAACAGCATCGGTCAGGACCGTCGTTTCCTGTTTAAATACATGCCGGAGCTGGAAGCCTACTTCCACTACC  >  NC_000913/4391840‑4391991                                                                           |                                                                              gATGGGCGATCGGGAAGCTGAACTGGCAACGCTCGAATTTTTAAAACAGTGGGTGCCTGCGGGAAAATCGCCGACTTGCgg                                                                         >  2:879158/1‑81 (MQ=255)   tGGGCGATCGGGAAGCTGAACTGGCAACGCTCGAATTTTTAAAACAGTGGGTGCCTGCGGGAAAATCGCCGACTTGCGGt                                                                        >  1:879167/1‑80 (MQ=255)   tGGGCGATCGGGAAGCTGAACTGGCAACGCTCGAATTTTTAAAACAGTGGGTGCCTGCGGGAAAATCGCCGACTTGCGGTa                                                                       <  2:879112/81‑1 (MQ=255)      gCGATCGGGAAGCTGAACTGGCAACGCTCGAATTTTTAAAACAGTGGGTGCCTGCGGGAAAATCGCCGACTTGCGGTaa                                                                      >  1:879155/1‑79 (MQ=255)      gCGATCGGGAAGCTGAACTGGCAACGCTCGAATTTTTAAAACAGTGGGTGCCTGCGGGAAAATCGCCGACTTGCGGTaa                                                                      >  1:879176/1‑79 (MQ=255)        gATCGGGAAGCTGAACTGGCAACGCTCGAATTTTTAAAACAGTGGGTGCCTGCGGGAAAATCGCCGACTTGCGGTAACAg                                                                   >  1:879156/1‑80 (MQ=255)            gggAAGCTGAACTGGCAACGCTCGAATTTTTAAAACAGTGGGTGCCTGCGGGAAAATCGCCGACTTGCGGTAACAGCATc                                                               <  1:879113/80‑1 (MQ=255)               aaGCTGAACTGGCAACGCTCGAATTTTTAAAACAGTGGGTGCCTGCGGGAAAATCGCCGACTTGCGGTAACAGCATCGGt                                                            >  1:879168/1‑80 (MQ=255)               aaGCTGAACTGGCAACGCTCGAATTTTTAAAACAGTGGGTGCCTGCGGGAAAATCGCCGACTTGCGGTAACAGCATCGGTc                                                           <  2:879115/81‑1 (MQ=255)               aaGCTGAACTGGCAACGCTCGAATTTTTAAAACAGTGGGTGCCTGCGGGAAAATCGCCGACTTGCGGTAACAGCATCGGTc                                                           <  1:879114/81‑1 (MQ=255)                aGCTGAACTGGCAACGCTCGAATTTTTAAAACAGTGGGTGCCTGCGGGAAAATCGCCGACTTGCGGTAACAGCATCGGTCa                                                          <  2:879116/81‑1 (MQ=255)                     aaCTGGCAACGCTCGAATTTTTAAAACAGTGGGTGCCTGCGGGAAAATCGCCGACTTGCGGTAACAGCATCGGTCAGGAcc                                                     <  1:879117/81‑1 (MQ=255)                       cTGGCAACGCTCGAATTTTTAAAACAGTGGGTGCCTGCGGGAAAATCGCCGACTTGCGGTAACAGCATCGGTCAGGACcgt                                                   <  2:879118/81‑1 (MQ=255)                         ggCAACGCTCGAATTTTTAAAACAGTGGGTGCCTGCGGGAAAATCGCCGACTTGCGGTAACAGCATCGGTCAGGACcgtcg                                                 >  2:879173/1‑81 (MQ=255)                         ggCAACGCTCGAATTTTTAAAACAGTGGGTGCCTGCGGGAAAATCGCCGACTTGCGGTAACAGCATCGGTCAGGACcgtcg                                                 >  2:879170/1‑81 (MQ=255)                         ggCAACGCTCGAATTTTTAAAACAGTGGGTGCCTGCGGGAAAATCGCCGACTTGCGGTAACAGCATCGGTCAGGACcgtcg                                                 >  2:879174/1‑81 (MQ=255)                          gCAACGCTCGAATTTTTAAAACAGTGGGTGCCTGCGGGAAAATCGCCGACTTGCGGTAACAGCATCGGTCAGGACcgtc                                                  <  2:879119/79‑1 (MQ=255)                           cAACGCTCGAATTTTTAAAACAGTGGGTGCCTGCGGGAAAATCGCCGACTTGCGGTAACAGCATCGGTCAGGACCGTCGtt                                               >  2:879177/1‑81 (MQ=255)                            aaCGCTCGAATTTTTAAAACAGTGGGTGCCTGCGGGAAAATCGCCGACTTGCGGTAACAGCATCGGTCAGGACCGTCGttt                                              <  1:879120/81‑1 (MQ=255)                             aCGCTCGAATTTTTAAAACAGTGGGTGCCTGCGGGAAAATCGCCGACTTGCGGTAACAGCATCGGTCAGGACCGTCGTTTc                                             >  1:879164/1‑81 (MQ=255)                                    aaTTTTTAAAACAGTGGGTGCCTGCGGGAAAATCGCCGACTTGCGGTAACAGCATCGGTCAGGACCGTCGTTTCCTGttt                                       >  1:879171/1‑80 (MQ=255)                                         ttAAAACAGTGGGTGCCTGCGGGAAAATCGCCGACTTGCGGTAACAGCATCGGTCAGGACCGTCGTTTCCTGTTTAAATa                                  <  2:879121/80‑1 (MQ=255)                                          tAAAACAGTGGGTGCCTGCGGGAAAATCGCCGACTTGCGGTAACAGCATCGGTCAGGACCGTCGTTTCCTGTTTAAATACa                                >  1:879161/1‑81 (MQ=255)                                             aaCAGTGGGTGCCTGCGGGAAAATCGCCGACTTGCGGTAACAGCATCGGTCAGGACCGTCGTTTCCTGTTTAAATACATGc                             <  1:879122/81‑1 (MQ=255)                                              aCAGTGGGTGCCTGCGGGAAAATCGCCGACTTGCGGTAACAGCATCGGTCAGGACCGTCGTTTCCTGTTTAAATACATGcc                            <  2:879123/81‑1 (MQ=255)                                                     gTGCCTGCGGGAAAATCGCCGACTTGCGGTAACAGCATCGGTCAGGACCGTCGTTTCCTGTTTAAATACATGCCGGAGCTg                     >  1:879182/1‑81 (MQ=255)                                                      tGCCTGCGGGAAAATCGCCGACTTGCGGTAACAGCATCGGTCAGGACCGTCGTTTCCTGTTTAAATACATGCCGGAGCTgg                    <  1:879124/81‑1 (MQ=255)                                                        ccTGCGGGAAAATCGCCGACTTGCGGTAACAGCATCGGTCAGGACCGTCGTTTCCTGTTTAAATACATGCCGGAGCTgg                    >  2:879172/1‑79 (MQ=255)                                                           gCGGGAAAATCGCCGACTTGCGGTAACAGCATCGGTCAGGACCGTCGTTTCCTGTTTAAATACATGCCGGAGCTGGAAGcc               >  2:879169/1‑81 (MQ=255)                                                           gCGGGAAAATCGCCGACTTGCGGTAACAGCATCGGTCAGGACCGTCGTTTCCTGTTTAAATACATGCCGGAGCTGGAAGcc               >  1:879165/1‑81 (MQ=255)                                                            cGGGAAAATCGCCGACTTGCGGTAACAGCATCGGTCAGGACCGTCGTTTCCTGTTTAAATACATGCCGGAGCTGGAAGcc               >  1:879178/1‑80 (MQ=255)                                                            cGGGAAAATCGCCGACTTGCGGTAACAGCATCGGTCAGGACCGTCGTTTCCTGTTTAAATACATGCCGGAGCTGGAAGCCt              <  2:879125/81‑1 (MQ=255)                                                                 aaaTCGCCGACTTGCGGTAACAGCATCGGTCAGGACCGTCGTTTCCTGTTTAAATACATGCCGGAGCTGGAAGCCTACTTc         <  1:879127/81‑1 (MQ=255)                                                                 aaaTCGCCGACTTGCGGTAACAGCATCGGTCAGGACCGTCGTTTCCTGTTTAAATACATGCCGGAGCTGGAAGCCTACTTc         <  1:879126/81‑1 (MQ=255)                                                                    tCGCCGACTTGCGGTAACAGCATCGGTCAGGACCGTCGTTTCCTGTTTAAATACATGCCGGAGCTGGAAGCCTACTTCCAc      <  2:879128/81‑1 (MQ=255)                                                                      gCCGACTTGCGGTAACAGCATCGGTCAGGACCGTCGTTTCCTGTTTAAATACATGCCGGAGCTGGAAGCCTACTTCCACt     <  1:879129/80‑1 (MQ=255)                                                                        cGACTTGCGGTAACAGCATCGGTCAGGACCGTCGTTTCCTGTTTAAATACATGCCGGAGCTGGAAGCCTACTTCCACTAcc  <  2:879130/81‑1 (MQ=255)                                                                           |                                                                              GATGGGCGATCGGGAAGCTGAACTGGCAACGCTCGAATTTTTAAAACAGTGGGTGCCTGCGGGAAAATCGCCGATTTGCGGTAACAGCATCGGTCAGGACCGTCGTTTCCTGTTTAAATACATGCCGGAGCTGGAAGCCTACTTCCACTACC  >  NC_000913/4391840‑4391991

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑