Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	81,437	A→G	R174R (CGT→CGC)	leuB ←	3‑isopropylmalate dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	81,437	0	A	G	100.0%	181.6 / NA	49	R174R (CGT→CGC)	leuB	3‑isopropylmalate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (25/24);  total (25/24)

CAGCACGTTGGCTTTATCGATCGACGTCACTTTGTGGCGACGCTTGCGAGCAGATTCAAACGCGATGCGGGCGATACGTTCGATCTCAAAACGGTGATACACCTCGGTATCAAAGGCTTTTTCATATTGTCCGCTACCTTCGCGGCCTTTT  >  NC_000913/81362‑81512                                                                            |                                                                            cagcaCGTTGGCTTTATCGATCGACGTCACTTTGTGGCGACGCTTGCGAGCAGATTCAAACGCGATGCGGGCGATGCGTTc                                                                        <  2:18413/81‑1 (MQ=255) cagcaCGTTGGCTTTATCGATCGACGTCACTTTGTGGCGACGCTTGCGAGCAGATTCAAACGCGATGCGGGCGATGCGTTc                                                                        <  2:18414/81‑1 (MQ=255)  agcaCGTTGGCTTTATCGATCGACGTCACTTTGTGGCGACGCTTGCGAGCAGATTCAAACGCGATGCGGGCGATGCGTTc                                                                        >  1:18469/1‑80 (MQ=255)  agcaCGTTGGCTTTATCGATCGACGTCACTTTGTGGCGACGCTTGCGAGCAGATTCAAACGCGATGCGGGCGATGCGTTc                                                                        >  1:18473/1‑80 (MQ=255)  agcaCGTTGGCTTTATCGATCGACGTCACTTTGTGGCGACGCTTGCGAGCAGATTCAAACGCGATGCGGGCGATGCGTTCg                                                                       <  2:18415/81‑1 (MQ=255)     aCGTTGGCTTTATCGATCGACGTCACTTTGTGGCGACGCTTGCGAGCAGATTCAAACGCGATGCGGGCGATGCGTTCGAtc                                                                    >  1:18487/1‑81 (MQ=255)       gTTGGCTTTATCGATCGACGTCACTTTGTGGCGACGCTTGCGAGCAGATTCAAACGCGATGCGGGCGATGCGTTCGAtctc                                                                  <  1:18416/81‑1 (MQ=255)       gTTGGCTTTATCGATCGACGTCACTTTGTGGCGACGCTTGCGAGCAGATTCAAACGCGATGCGGGCGATGCGTTCGAtct                                                                   >  2:18465/1‑80 (MQ=255)        ttGGCTTTATCGATCGACGTCACTTTGTGGCGACGCTTGCGAGCAGATTCAAACGCGATGCGGGCGATGCGTTCGAtctc                                                                  >  2:18479/1‑80 (MQ=255)          ggCTTTATCGATCGACGTCACTTTGTGGCGACGCTTGCGAGCAGATTCAAACGCGATGCGGGCGATGCGTTCGATCTCaa                                                                <  2:18417/80‑1 (MQ=255)           gCTTTATCGATCGACGTCACTTTGTGGCGACGCTTGCGAGCAGATTCAAACGCGATGCGGGCGATGCGTTCGATCTCaaaa                                                              >  1:18493/1‑81 (MQ=255)            cTTTATCGATCGACGTCACTTTGTGGCGACGCTTGCGAGCAGATTCAAACGCGATGCGGGCGATGCGTTCGATCTCAAAAc                                                             <  2:18418/81‑1 (MQ=255)            cTTTATCGATCGACGTCACTTTGTGGCGACGCTTGCGAGCAGATTCAAACGCGATGCGGGCGATGCGTTCGATCTCAAAAc                                                             >  2:18484/1‑81 (MQ=255)               tATCGATCGACGTCACTTTGTGGCGACGCTTGCGAGCAGATTCAAACGCGATGCGGGCGATGCGTTCGATCTCAAAACGGt                                                          >  2:18489/1‑81 (MQ=255)                atcgatcgACGTCACTTTGTGGCGACGCTTGCGAGCAGATTCAAACGCGATGCGGGCGATGCGTTCGATCTCAAAACGGt                                                          <  2:18419/80‑1 (MQ=255)                   gatcgaCGTCACTTTGTGGCGACGCTTGCGAGCAGATTCAAACGCGATGCGGGCGATGCGTTCGATCTCAAAACGGTGATa                                                      >  1:18492/1‑81 (MQ=255)                   gatcgaCGTCACTTTGTGGCGACGCTTGCGAGCAGATTCAAACGCGATGCGGGCGATGCGTTCGATCTCAAAACGGTGATa                                                      >  2:18482/1‑81 (MQ=255)                    atcgaCGTCACTTTGTGGCGACGCTTGCGAGCAGATTCAAACGCGATGCGGGCGATGCGTTCGATCTCAAAACGGTGATa                                                      >  1:18490/1‑80 (MQ=255)                       gaCGTCACTTTGTGGCGACGCTTGCGAGCAGATTCAAACGCGATGCGGGCGATGCGTTCGATCTCAAAACGGTGATACAcc                                                  >  1:18497/1‑81 (MQ=255)                       gaCGTCACTTTGTGGCGACGCTTGCGAGCAGATTCAAACGCGATGCGGGCGATGCGTTCGATCTCAAAACGGTGATACAcc                                                  >  2:18500/1‑81 (MQ=255)                          gTCACTTTGTGGCGACGCTTGCGAGCAGATTCAAACGCGATGCGGGCGATGCGTTCGATCTCAAAACGGTGATACACCTCg                                               >  2:18491/1‑81 (MQ=255)                             aCTTTGTGGCGACGCTTGCGAGCAGATTCAAACGCGATGCGGGCGATGCGTTCGATCTCAAAACGGTGATACACCTCGGt                                             <  1:18420/80‑1 (MQ=255)                             aCTTTGTGGCGACGCTTGCGAGCAGATTCAAACGCGATGCGGGCGATGCGTTCGATCTCAAAACGGTGATACACCTCGGt                                             <  2:18421/80‑1 (MQ=255)                             aCTTTGTGGCGACGCTTGCGAGCAGATTCAAACGCGATGCGGGCGATGCGTTCGATCTCAAAACGGTGATACACCTCGGTa                                            <  2:18422/81‑1 (MQ=255)                                ttGTGGCGACGCTTGCGAGCAGATTCAAACGCGATGCGGGCGATGCGTTCGATCTCAAAACGGTGATACACCTCGGTATc                                          >  2:18456/1‑80 (MQ=255)                                    ggCGACGCTTGCGAGCAGATTCAAACGCGATGCGGGCGATGCGTTCGATCTCAAAACGGTGATACACCTCGGTATCAAAgg                                     >  2:18488/1‑81 (MQ=255)                                       gACGCTTGCGAGCAGATTCAAACGCGATGCGGGCGATGCGTTCGATCTCAAAACGGTGATACACCTCGGTATCAAAGGCtt                                  >  2:18476/1‑81 (MQ=255)                                           cTTGCGAGCAGATTCAAACGCGATGCGGGCGATGCGTTCGATCTCAAAACGGTGATACACCTCGGTATCAAAGGCTTTTTc                              <  1:18423/81‑1 (MQ=255)                                              gCGAGCAGATTCAAACGCGATGCGGGCGATGCGTTCGATCTCAAAACGGTGATACACCTCGGTATCAAAGGCTTTTTCat                            <  1:18424/80‑1 (MQ=255)                                               cGAGCAGATTCAAACGCGATGCGGGCGATGCGTTCGATCTCAAAACGGTGATACACCTCGGTATCAAAGGCTTTTTCatat                          <  1:18425/81‑1 (MQ=255)                                               cGAGCAGATTCAAACGCGATGCGGGCGATGCGTTCGATCTCAAAACGGTGATACACCTCGGTATCAAAGGCTTTTTCatat                          <  1:18427/81‑1 (MQ=255)                                               cGAGCAGATTCAAACGCGATGCGGGCGATGCGTTCGATCTCAAAACGGTGATACACCTCGGTATCAAAGGCTTTTTCata                           <  1:18426/80‑1 (MQ=255)                                                 aGCAGATTCAAACGCGATGCGGGCGATGCGTTCGATCTCAAAACGGTGATACACCTCGGTATCAAAGGCTTTTTCATATTg                        >  1:18504/1‑81 (MQ=255)                                                   cAGATTCAAACGCGATGCGGGCGATGCGTTCGATCTCAAAACGGTGATACACCTCGGTATCAAAGGCTTTTTCATATTGTc                      <  2:18428/81‑1 (MQ=255)                                                    aGATTCAAACGCGATGCGGGCGATGCGTTCGATCTCAAAACGGTGATACACCTCGGTATCAAAGGCTTTTTCATATTGTcc                     <  1:18429/81‑1 (MQ=255)                                                      aTTCAAACGCGATGCGGGCGATGCGTTCGATCTCAAAACGGTGATACACCTCGGTATCAAAGGCTTTTTCATATTGTCCGc                   <  2:18430/81‑1 (MQ=255)                                                        tCAAACGCGATGCGGGCGATGCGTTCGATCTCAAAACGGTGATACACCTCGGTATCAAAGGCTTTTTCATATTGTCCGCTa                 >  1:18483/1‑81 (MQ=255)                                                          aaaCGCGATGCGGGCGATGCGTTCGATCTCAAAACGGTGATACACCTCGGTATCAAAGGCTTTTTCATATTGTCCGCTAcc               >  2:18505/1‑81 (MQ=255)                                                          aaaCGCGATGCGGGCGATGCGTTCGATCTCAAAACGGTGATACACCTCGGTATCAAAGGCTTTTTCATATTGTCCGCTAc                <  1:18431/80‑1 (MQ=255)                                                           aaCGCGATGCGGGCGATGCGTTCGATCTCAAAACGGTGATACACCTCGGTATCAAAGGCTTTTTCATATTGTCCGCTACCt              >  2:18494/1‑81 (MQ=255)                                                             cgcgATGCGGGCGATGCGTTCGATCTCAAAACGGTGATACACCTCGGTATCAAAGGCTTTTTCATATTGTCCGCTACCTTc            <  2:18432/81‑1 (MQ=255)                                                              gcgATGCGGGCGATGCGTTCGATCTCAAAACGGTGATACACCTCGGTATCAAAGGCTTTTTCATATTGTCCGCTACCTTcg           >  1:18503/1‑81 (MQ=255)                                                                gATGCGGGCGATGCGTTCGATCTCAAAACGGTGATACACCTCGGTATCAAAGGCTTTTTCATATTGTCCGCTACCTTcgcg         >  1:431678/1‑81 (MQ=255)                                                                  tGCGGGCGATGCGTTCGATCTCAAAACGGTGATACACCTCGGTATCAAAGGCTTTTTCATATTGTCCGCTACCTTCGCGGc       <  2:18433/81‑1 (MQ=255)                                                                    cGGGCGATGCGTTCGATCTCAAAACGGTGATACACCTCGGTATCAAAGGCTTTTTCATATTGTCCGCTACCTTCGCGGCCt     <  1:18434/81‑1 (MQ=255)                                                                    cGGGCGATGCGTTCGATCTCAAAACGGTGATACACCTCGGTATCAAAGGCTTTTTCATATTGTCCGCTACCTTCGCGGCCt     <  1:733838/81‑1 (MQ=255)                                                                     gggCGATGCGTTCGATCTCAAAACGGTGATACACCTCGGTATCAAAGGCTTTTTCATATTGTCCGCTACCTTCGCGGCCtt    >  2:18496/1‑81 (MQ=255)                                                                      ggCGATGCGTTCGATCTCAAAACGGTGATACACCTCGGTATCAAAGGCTTTTTCATATTGTCCGCTACCTTCGCGGCCttt   <  1:18435/81‑1 (MQ=255)                                                                       gCGATGCGTTCGATCTCAAAACGGTGATACACCTCGGTATCAAAGGCTTTTTCATATTGTCCGCTACCTTCGCGGCCtttt  >  1:18495/1‑81 (MQ=255)                                                                            |                                                                            CAGCACGTTGGCTTTATCGATCGACGTCACTTTGTGGCGACGCTTGCGAGCAGATTCAAACGCGATGCGGGCGATACGTTCGATCTCAAAACGGTGATACACCTCGGTATCAAAGGCTTTTTCATATTGTCCGCTACCTTCGCGGCCTTTT  >  NC_000913/81362‑81512

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑