| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 4,410,422 | T→C | V97A (GTT→GCT) | yjfI → | conserved protein YjfI |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,410,422 | 0 | T | C | 95.0% | 61.6 / ‑3.6 | 20 | V97A (GTT→GCT) | yjfI | conserved protein YjfI |
| Reads supporting (aligned to +/- strand): ref base T (0/1); new base C (9/10); total (9/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.54e-01 | |||||||||||
TCTTATTAAGAAATCAGAAGATGATGCCGTTGTCATCGGTAGGGATCTCCAGCGTACAACAGGAAGAGTATTACATTGTTTTCGGGGCGTTATCGCTTAAATCTTCTCTTGAAGATATCCTGCTTGAGATAACCTCGCTGGTAGATAACGCATTG > NC_000913/4410344‑4410498 | tcttATTAAGAAATCAGAAGATGATGCCGTTGTCATCGGTAGGGATCTCCAGCGTACAACAGGAAGAGTATTACATTGctt > 1:883875/1‑81 (MQ=255) attaAGAAATCAGAAGATGATGCCGTTGTCATCGGTAGGGATCTCCAGCGTACAACAGGAAGAGTATTACATTGCTTTc < 1:883851/79‑1 (MQ=255) cAGAAGATGATGCCGTTGTCATCGGTAGGGATCTCCAGCGTACAACAGGAAGAGTATTACATTGCTTTCGGGGCGTTATCg > 2:883886/1‑81 (MQ=255) gaagaTGATGCCGTTGTCATCGGTAGGGATCTCCAGCGTACAACAGGAAGAGTATTACATTGCTTTCGGGGCGTTATCGCt > 2:883889/1‑81 (MQ=255) gatgatGCCGTTGTCATCGGTAGGGATCTCCAGCGTACAACAGGAAGAGTATTACATTGCTTTCGGGGCGTTATCGCTTaa < 2:883852/81‑1 (MQ=255) atgatgCCGTTGTCATCGGTAGGGATCTCCAGCGTACAACAGGAAGAGTATTACATTGCTTTCGGGGCGTTATCGCTTaaa > 2:883883/1‑81 (MQ=255) ccGTTGTCATCGGTAGGGATCTCCAGCGTACAACAGGAAGAGTATTACATTGCTTTCGGGGCGTTATCGCTTAAAtcttc < 2:883853/80‑1 (MQ=255) cGTTGTCATCGGTAGGGATCTCCAGCGTACAACAGGAAGAGTATTACATTGCTTTCGGGGCGTTATCGCTTAAATCTtctc > 1:883881/1‑81 (MQ=255) ttGTCATCGGTAGGGATCTCCAGCGTACAACAGGAAGAGTATTACATTGCTTTCGGGGCGTTATCGCTTAAATCTTCTCtt > 1:883879/1‑81 (MQ=255) cAGCGTACAACAGGAAGAGTATTACATTGTTTTCGGGGCGTTATCGCTTAAATCTTCTCTTGAAGATATCCTGCtt < 2:883854/76‑1 (MQ=255) cAGCGTACAACAGGAAGAGTATTACATTGCTTTCGGGGCGTTATCGCTTAAATCTTCTCTTGAAGATATCCTGCTTgaga > 1:883880/1‑80 (MQ=255) cAGCGTACAACAGGAAGAGTATTACATTGCTTTCGGGGCGTTATCGCTTAAATCTTCTCTTGAAGATATCCTGCTTg < 1:883856/77‑1 (MQ=255) cAGCGTACAACAGGAAGAGTATTACATTGCTTTCGGGGCGTTATCGCTTAAATCTTCTCTTGAAGATATCCTGCTTg < 2:883857/77‑1 (MQ=255) cAGCGTAAAACAGGAAGGGTATTACATTGCTTTCGGGGCGTTATCGCTTAAATCTTCTCTTGAAGATATCCTGCTTg < 2:883855/77‑1 (MQ=255) gTACAACAGGAAGAGTATTACATTGCTTTCGGGGCGTTATCGCTTAAATCTTCTCTTGAAGATATCCTGCTTGAGATAAcc < 2:883858/81‑1 (MQ=255) caacaGGAAGAGTATTACATTGCTTTCGGGGCGTTATCGCTTAAATCTTCTCTTGAAGATATCCTGCTTGAGATAACCTc < 2:883859/80‑1 (MQ=255) aacaGGAAGAGTATTACATTGCTTTCGGGGCGTTATCGCTTAAATCTTCTCTTGAAGATATCCTGCTTGAGATAACCTCGc > 1:883882/1‑81 (MQ=255) caGGAAGAGTATTACATTGCTTTCGGGGCGTTATCGCTTAAATCTTCTCTTGAAGATATCCTGCTTGAGATAACCTCGCTg > 2:883898/1‑81 (MQ=255) ttACATTGCTTTCGGGGCGTTATCGCTTAAATCTTCTCTTGAAGATATCCTGCTTGAGATAACCTCGCTGGTAGATAACGc < 1:883860/81‑1 (MQ=255) cATTGCTTTCGGGGCGTTATCGCTTAAATCTTCTCTTGAAGATATCCTGCTTGAGATAACCTCGCTGGTAGATAACGCAtt < 2:883861/81‑1 (MQ=255) aTTGCTTTCGGGGCGTTATCGCTTAAATCTTCTCTTGAAGATATCCTGCTTGAGATAACCTCGCTGGTAGATAACGCATTg > 2:883903/1‑81 (MQ=255) | TCTTATTAAGAAATCAGAAGATGATGCCGTTGTCATCGGTAGGGATCTCCAGCGTACAACAGGAAGAGTATTACATTGTTTTCGGGGCGTTATCGCTTAAATCTTCTCTTGAAGATATCCTGCTTGAGATAACCTCGCTGGTAGATAACGCATTG > NC_000913/4410344‑4410498 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |