Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,418,094	G→A	I120I (ATC→ATT)	ulaR ←	DNA‑binding transcriptional repressor UlaR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,418,094	0	G	A	100.0%	88.2 / NA	25	I120I (ATC→ATT)	ulaR	DNA‑binding transcriptional repressor UlaR
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (15/10);  total (15/10)

TTGTACTGTCCGCCCATAATGATCACGCTGTCATGTTCTTGATCGATCAGGTAATTTGCCAGCGGTAGATAATTAGTGATGATTTGCACTGGCTTGCCACACATTTCCCGCCCAAGCAGAAACGCGGTGGAGCCGCAGTTGATGACTACGCTT  >  NC_000913/4418017‑4418169                                                                              |                                                                            ttgtACTGTCCGCCCATAATGATCACGCTGTCATGTTCTTGATCGATCAGGTAATTTGCCAGCGGTAGATAATTAGtaat                                                                           <  1:885618/80‑1 (MQ=255)  tgtACTGTCCGCCCATAATGATCACGCTGTCATGTTCTTGATCGATCAGGTAATTTGCCAGCGGTAGATAATTAGtaatga                                                                         >  2:885658/1‑81 (MQ=255)   gtACTGTCCGCCCATAATGATCACGCTGTCATGTTCTTGATCGATCAGGTAATTTGCCAGCGGTAGATAATTAGtaatga                                                                         <  1:885619/80‑1 (MQ=255)   gtACTGTCCGCCCATAATGATCACGCTGTCATGTTCTTGATCGATCAGGTAATTTGCCAGCGGTAGATAATTAGtaatga                                                                         <  2:885620/80‑1 (MQ=255)      cTGTCCGCCCATAATGATCACGCTGTCATGTTCTTGATCGATCAGGTAATTTGCCAGCGGTAGATAATTAGTAATGATTTg                                                                     <  2:885621/81‑1 (MQ=255)        gTCCGCCCATAATGATCACGCTGTCATGTTCTTGATCGATCAGGTAATTTGCCAGCGGTAGATAATTAGTAATGATTTGCa                                                                   >  1:885659/1‑81 (MQ=255)         tCCGCCCATAATGATCACGCTGTCATGTTCTTGATCGATCAGGTAATTTGCCAGCGGTAGATAATTAGTAATGATTTGCAc                                                                  <  2:885622/81‑1 (MQ=255)          ccGCCCATAATGATCACGCTGTCATGTTCTTGATCGATCAGGTAATTTGCCAGCGGTAGATAATTAGTAATGATTTGCACt                                                                 >  2:885671/1‑81 (MQ=255)             cccATAATGATCACGCTGTCATGTTCTTGATCGATCAGGTAATTTGCCAGCGGTAGATAATTAGTAATGATTTGCACTGGc                                                              <  2:885623/81‑1 (MQ=255)                 tAATGATCACGCTGTCATGTTCTTGATCGATCAGGTAATTTGCCAGCGGTAGATAATTAGTAATGATTTGCACTGGCTTGc                                                          >  2:885665/1‑81 (MQ=255)                  aaTGATCACGCTGTCATGTTCTTGGTCGATCAGGTAATTTGCCAGCGGTAGATAATTAGTAATTATTTGCACTGGCTTGc                                                          >  1:885668/1‑80 (MQ=255)                    tGATCACGCTGTCATGTTCTTGATCGATCAGGTAATTTGCCAGCGGTAGATAATTAGTAATGATTTGCACTGGCTTGCcac                                                       <  2:885624/81‑1 (MQ=255)                      aTCACGCTGTCATGTTCTTGATCGATCAGGTAATTTGCCAGCGGTAGATAATTAGTAATGATTTGCACTGGCTTGCcaca                                                      >  1:885666/1‑80 (MQ=255)                            cTGTCATGTTCTTGATCGATCAGGTAATTTGCCAGCGGTAGATAATTAGTAATGATTTGCACTGGCTTGCCACACATTTcc                                               <  2:885625/81‑1 (MQ=255)                              gTCATGTTCTTGATCGATCAGGTAATTTGCCAGCGGTAGATAATTAGTAATGATTTGCACTGGCTTGCCACACATTTCCCg                                             >  2:885663/1‑81 (MQ=255)                                         gatcgatcAGGTAATTTGCCAGCGGTAGATAATTAGTAATGATTTGCACTGGCTTGCCACACATTTCCCGCCCAAGCAGa                                   >  1:885656/1‑80 (MQ=255)                                         gatcgatcAGGTAATTTGCCAGCGGTAGATAATTAGTAATGATTTGCACTGGCTTGCCACACATTTCCCGCCCAAGCAGa                                   >  2:885664/1‑80 (MQ=255)                                          atcgatcAGGTAATTTGCCAGCGGTAGATAATTAGTAATGATTTGCACTGGCTTGCCACACATTTCCCGCCCAAGCAGaa                                  <  1:885626/80‑1 (MQ=255)                                           tcgatcAGGTAATTTGCCAGCGGTAGATAATTAGTAATGATTTGCACTGGCTTGCCACACATTTCCCGCCCAAGCAGAAAc                                <  2:885627/81‑1 (MQ=255)                                               tcAGGTAATTTGCCAGCGGTAGATAATTAGTAATGATTTGCACTGGCTTGCCACACATTTCCCGCCCAAGCAGAAACGCgg                            <  1:885628/81‑1 (MQ=255)                                                  ggTAATTTGCCAGCGGTAGATAATTAGTAATGATTTGCACTGGCTTGCCACACATTTCCCGCCCAAGCAGAAACGCGGTg                          >  1:885670/1‑80 (MQ=255)                                                   gTAATTTGCCAGCGGTAGATAATTAGTAATGATTTGCACTGGCTTGCCACACATTTCCCGCCCAAGCAGAAACGCGGTGGa                        <  1:885629/81‑1 (MQ=255)                                                            cAGCGGTAGATAATTAGTAATGATTTGCACTGGCTTGCCACACATTTCCCGCCCAAGCAGAAACGCGGTGGAGCCGCAGtt               >  1:885662/1‑81 (MQ=255)                                                             aGCGGTAGATAATTAGTAATGATTTGCACTGGCTTGCCACACATTTCCCGCCCAAGCAGAAACGCGGTGGAGCCGCAGtt               >  2:885675/1‑80 (MQ=255)                                                                ggTAGATAATTAGTAATGATTTGCACTGGCTTGCCACACATTTCCCGCCCAAGCAGAAACGCGGTGGAGCCGCAGTtgat            >  1:885674/1‑80 (MQ=255)                                                                     aTAATTAGTAATGATTTGCACTGGCTTGCCACACATTTCCCGCCCAAGCAGAAACGCGGTGGAGCCGCAGTTGATGACTAc      >  2:885669/1‑81 (MQ=255)                                                                     aTAATTAGTAATGATTTGCACTGGCTTGCCACACATTTCCCGCCCAAGCAGAAACGCGGTGGAGCCGCAGTTGATGACTAc      >  2:885678/1‑81 (MQ=255)                                                                        aTTAGTAATGATTTGCACTGGCTTGCCACACATTTCCCGCCCAAGCAGAAACGCGGTGGAGCCGCAGTTGATGACTACGCt   >  2:885673/1‑81 (MQ=255)                                                                         ttAGTAATGATTTGCACTGGCTTGCCACACATTTCCCGCCCAAGCAGAAACGCGGTGGAGCCGCAGTTGATGACTACGCtt  <  2:885630/81‑1 (MQ=255)                                                                              |                                                                            TTGTACTGTCCGCCCATAATGATCACGCTGTCATGTTCTTGATCGATCAGGTAATTTGCCAGCGGTAGATAATTAGTGATGATTTGCACTGGCTTGCCACACATTTCCCGCCCAAGCAGAAACGCGGTGGAGCCGCAGTTGATGACTACGCTT  >  NC_000913/4418017‑4418169

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑