Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,460,132	T→C	H78R (CAT→CGT)	nrdG ←	anaerobic ribonucleoside‑triphosphate reductase activating protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,460,132	0	T	C	100.0%	112.2 / NA	31	H78R (CAT→CGT)	nrdG	anaerobic ribonucleoside‑triphosphate reductase activating protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (16/15);  total (16/15)

ACCCAGATGTCTTTACCCGGACACTCGGCGCGGATGCGTTGTACCAGTTTCAGAATATCCGGCACGTTTTGCGGATGCAGCGGATCGCCGCCGGAGAGGGAAATACCCTGGCGTTTGATACGAGTGTCATTCAGATCGTTAATGATCTGGTCT  >  NC_000913/4460057‑4460209                                                                            |                                                                              acCCAGATGTCTTTACCCGGACACTCGGCGCGGATGCGTTGTACCAGTTTCAGAATATCCGGCACGTTTTGCGGACGCAGc                                                                          >  2:895648/1‑81 (MQ=255)      gATGTCTTTACCCGGACACTCGGCGCGGATGCGTTGTACCAGTTTCAGAATATCCGGCACGTTTTGCGGACGCAGCGGATc                                                                     <  2:895601/81‑1 (MQ=255)           cTTTACCCGGACACTCGGCGCGGATGCGTTGTACCAGTTTCAGAATATCCGGCACGTTTTGCGGACGCAGCGGATcgccgc                                                                >  2:895642/1‑81 (MQ=255)               aCCCGGACACTCGGCGCGGATGCGTTGTACCAGTTTCAGAATATCCGGCACGTTTTGCGGACGCAGCGGATCGCCGCCgg                                                             <  2:895602/80‑1 (MQ=255)                 ccGGACACTCGGCGCGGATGCGTTGTACCAGTTTCAGAATATCCGGCACGTTTTGCGGACGCAGCGGATCGCCGCCGga                                                            >  1:895660/1‑79 (MQ=255)                      cacTCGGCGCGGATGCGTTGTACCAGTTTCAGAATATCCGGCACGTTTTGCGGACGCAGCGGATCGCCGCCGGAGAGGGa                                                      >  1:895666/1‑80 (MQ=255)                        cTCGGCGCGGATGCGTTGTACCAGTTTCAGAATATCCGGCACGTTTTGCGGACGCAGCGGATCGCCGCCGGAGAGGGAAAt                                                   <  2:895603/81‑1 (MQ=255)                          cGGCGCGGATGCGTTGTACCAGTTTCAGAATATCCGGCACGTTTTGCGGACGCAGCGGATCGCCGCCGGAGAGGGAAATAc                                                 >  2:895652/1‑81 (MQ=255)                             cgcgGATGCGTTGTACCAGTTTCAGAATATCCGGCACGTTTTGCGGACGCAGCGGATCGCCGCCGGAGAGGGAAATACCCt                                              >  1:895663/1‑81 (MQ=255)                              gcgGATGCGTTGTACCAGTTTCAGAATATCCGGCACGTTTTGCGGACGCAGCGGATCGCCGCCGGAGAGGGAAATACCCTg                                             >  1:895662/1‑81 (MQ=255)                              gcgGATGCGTTGTACCAGTTTCAGAATATCCGGCACGTTTTGCGGACGCAGCGGATCGCCGCCGGAGAGGGAAATACCCTg                                             >  1:895661/1‑81 (MQ=255)                               cgGATGCGTTGTACCAGTTTCAGAATATCCGGCACGTTTTGCGGACGCAGCGGATCGCCGCCGGAGAGGGAAATACCCTgg                                            <  2:895604/81‑1 (MQ=255)                                  aTGCGTTGTACCAGTTTCAGAATATCCGGCACGTTTTGCGGACGCAGCGGATCGCCGCCGGAGAGGGAAATACCCTGGCGt                                         >  2:895649/1‑81 (MQ=255)                                    gCGTTGTACCAGTTTCAGAATATCCGGCACGTTTTGCGGACGCAGCGGATCGCCGCCGGAGAGGGAAATACCCTGGCGttt                                       >  1:895643/1‑81 (MQ=255)                                    gCGTTGTACCAGTTTCAGAATATCCGGCACGTTTTGCGGACGCAGCGGATCGCCGCCGGAGAGGGAAATACCCTGGCGttt                                       >  2:895650/1‑81 (MQ=255)                                     cGTTGTACCAGTTTCAGAATATCCGGCACGTTTTGCGGACGCAGCGGATCGCCGCCGGAGAGGGAAATACCCTGGCGttt                                       <  2:895605/80‑1 (MQ=255)                                     cGTTGTACCAGTTTCAGAATATCCGGCACGTTTTGCGGACGCAGCGGATCGCCGCCGGAGAGGGAAATACCCTGGCGttt                                       <  1:895606/80‑1 (MQ=255)                                         gTACCAGTTTCAGAATATCCGGCACGTTTTGCGGACGCAGCGGATCGCCGCCGGAGAGGGAAATACCCTGGCGTTTGATa                                   <  2:895607/80‑1 (MQ=255)                                          tACCAGTTTCAGAATATCCGGCACGTTTTGCGGACGCAGCGGATCGCCGCCGGAGAGGGAAATACCCTGGCGTTTGATAc                                  <  2:895608/80‑1 (MQ=255)                                            ccAGTTTCAGAATATCCGGCACGTTTTGCGGACGCAGCGGATCGCCGCCGGAGAGGGAAATACCCTGGCGTTTGATACGa                                <  2:895609/80‑1 (MQ=255)                                                    aGAATATCCGGCACGTTTTGCGGACGCAGCGGATCGCCGCCGGAGAGGGAAATACCCTGGCGTTTGATACGAGTGTCAtt                        >  2:895655/1‑80 (MQ=255)                                                     gAATATCCGGCACGTTTTGCGGACGCAGCGGATCGCCGCCGGAGAGGGAAATACCCTGGCGTTTGATACGAGTGTCAtt                        >  1:895668/1‑79 (MQ=255)                                                      aaTATCCGGCACGTTTTGCGGACGCAGCGGATCGCCGCCGGAGAGGGAAATACCCTGGCGTTTGATACGAGTGTCATTCAg                     <  1:895610/81‑1 (MQ=255)                                                      aaTATCCGGCACGTTTTGCGGACGCAGCGGATCGCCGCCGGAGAGGGAAATACCCTGGCGTTTGATACGAGTGTCATTCAg                     <  2:895611/81‑1 (MQ=255)                                                        tatCCGGCACGTTTTGCGGACGCAGCGGATCGCCGCCGGAGAGGGAAATACCCTGGCGTTTGATACGAGTGTCATTCAGAt                   <  2:895612/81‑1 (MQ=255)                                                           ccGGCACGTTTTGCGGACGCAGCGGATCGCCGCCGGAGAGGGAAATACCCTGGCGTTTGATACGAGTGTCATTCAGATCGt                >  2:895667/1‑81 (MQ=255)                                                              gCACGTTTTGCGGACGCAGCGGATCGCCGCCGGAGAGGGAAATACCCTGGCGTTTGATACGAGTGTCATTCAGATCGTTaa             <  2:895613/81‑1 (MQ=255)                                                               cACGTTTTGCGGACGCAGCGGATCGCCGCCGGAGAGGGAAATACCCTGGCGTTTGATACGAGTGTCATTCAGATCGTTAAt            >  1:895658/1‑81 (MQ=255)                                                                 cGTTTTGCGGACGCAGCGGATCGCCGCCGGAGAGGGAAATACCCTGGCGTTTGATACGAGTGTCATTCAGATCGTTAATGa          <  2:895614/81‑1 (MQ=255)                                                                  gTTTTGCGGACGCAGCGGATCGCCGCCGGAGAGGGAAATACCCTGGCGTTTGATACGAGTGTCATTCAGATCGTTAATGAt         >  1:895657/1‑81 (MQ=255)                                                                         ggACGCAGCGGATCGCCGCCGGAGTGGGAAATACCCTGGCGTTTGATACGAGTGTCATTCAGATCGTTAATGATCTGGTCt  <  2:895615/81‑1 (MQ=255)                                                                            |                                                                              ACCCAGATGTCTTTACCCGGACACTCGGCGCGGATGCGTTGTACCAGTTTCAGAATATCCGGCACGTTTTGCGGATGCAGCGGATCGCCGCCGGAGAGGGAAATACCCTGGCGTTTGATACGAGTGTCATTCAGATCGTTAATGATCTGGTCT  >  NC_000913/4460057‑4460209

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑