Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,479,585	C→T	intergenic (‑48/‑145)	yjgM ← / → yjgN	putative acetyltransferase YjgM/conserved inner membrane protein YjgN

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,479,585	0	C	T	100.0%	63.0 / NA	18	intergenic (‑48/‑145)	yjgM/yjgN	putative acetyltransferase YjgM/conserved inner membrane protein YjgN
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (10/8);  total (10/8)

TTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACATTTGTTTAATACCACTCTCTTCTTACGTACGCAAGCGACTTATAAAGTAATAAAGTTTTTGCCAGCCTATTACTGAT  >  NC_000913/4479507‑4479647                                                                               |                                                               ttACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACATTTGTTTAATACCAttc                                                              <  1:899822/81‑1 (MQ=255)              cggcgCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACATTTGTTTAATACCATTCTCTTCTtacgtac                                                 <  2:899823/81‑1 (MQ=255)              cggcgCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACATTTGTTTAATACCATTCTCTTCTtacgta                                                  <  2:899824/80‑1 (MQ=255)                    aaTGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACATTTGTTTAATACCATTCTCTTCTTACGTACGCAAGc                                           <  2:899825/81‑1 (MQ=255)                      tGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACATTTGTTTAATACCATTCTCTTCTTACGTACGCAAGCGa                                         >  2:899847/1‑81 (MQ=255)                          aTTCATAGTTTAACCCAATAAAAATAAAGTAATTATACATTTGTTTAATACCATTCTCTTCTTACGTACGCAAGCGACtt                                      <  1:899826/80‑1 (MQ=255)                          aTTCATAGTTTAACCCAATAAAAATAAAGTAATTATACATTTGTTTAATACCATTCTCTTCTTACGTACGCAAGCGACTta                                     >  2:899845/1‑81 (MQ=255)                          aTTCATAGTTTAACCCAATAAAAATAAAGTAATTATACATTTGTTTAATACCATTCTCTTCTTACGTACGCAAGCGACTta                                     >  2:899846/1‑81 (MQ=255)                          aTTCATAGTTTAACCCAATAAAAATAAAGTAATTATACATTTGTTTAATACCATTCTCTTCTTACGTACGCAAGCGACTta                                     >  2:899848/1‑81 (MQ=255)                           ttCATAGTTTAACCCAATAAAAATAAAGTAATTATACATTTGTTTAATACCATTCTCTTCTTACGTACGCAAGCGACTtat                                    <  2:899827/81‑1 (MQ=255)                                        ccAATAAAAATAAAGTAATTATACATTTGTTTAATACCATTCTCTTCTTACGTACGCAAGCGACTTATAAAGTAATAAAGt                       >  1:899853/1‑81 (MQ=255)                                             aaaaataaaGTAATTATACATTTGTTTAATACCATTCTCTTCTTACGTACGCAAGCGACTTATAAAGTAATAAAGTTTTTg                  >  1:899852/1‑81 (MQ=255)                                                 ataaaGTAATTATACATTTGTTTAATACCATTCTCTTCTTACGTACGCAAGCGACTTATAAAGTAATAAAGTTTTTGCCAg              >  1:899850/1‑81 (MQ=255)                                                    aaGTAATTATACATTTGTTTAATACCATTCTCTTCTTACGTACGCAAGCGACTTATAAAGTAATAAAGTTTTTGCCAGCCt           <  2:899828/81‑1 (MQ=255)                                                       tAATTATACATTTGTTTAATACCATTCTCTTCTTACGTACGCAAGCGACTTATAAAGTAATAAAGTTTTTGCCAGCCTAtt        <  1:899830/81‑1 (MQ=255)                                                       tAATTATACATTTGTTTAATACCATTCTCTTCTTACGTACGCAAGCGACTTATAAAGTAATAAAGTTTTTGCCAGCCTAtt        <  1:899829/81‑1 (MQ=255)                                                            ataCATTTGTTTAATACCATTCTCTTCTTACGTACGCAAGCGACTTATAAAGTAATAAAGTTTTTGCCAGCCTATTACTGa   >  1:899859/1‑81 (MQ=255)                                                              aCATTTGTTTAATGCCATTCTCTTCTTACGTACGCAAGCGACTTATAAAGTAATAAAGTTTTTGCCAGCCTATTACTGAt  >  2:899844/1‑80 (MQ=255)                                                               cATTTGTTTAATACCATTCTCTTCTTACGTACGCAAGCGACTTATAAAGTAATAAAGTTTTTGCCAGCCTATTACTGAt  >  2:899857/1‑79 (MQ=255)                                                                               |                                                               TTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACATTTGTTTAATACCACTCTCTTCTTACGTACGCAAGCGACTTATAAAGTAATAAAGTTTTTGCCAGCCTATTACTGAT  >  NC_000913/4479507‑4479647

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑