Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,480,873	G→A	G382S (GGC→AGC)	yjgN →	conserved inner membrane protein YjgN

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,480,873	0	G	A	100.0%	105.3 / NA	29	G382S (GGC→AGC)	yjgN	conserved inner membrane protein YjgN
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (20/9);  total (20/9)

CAAAAAATACGTATTTGCTGGGCGATTTGGATGACCTTCCTTTAATCAATAAAGAAGAACAACCAGATAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGATAAAAAAGGCCGGAGCATGC  >  NC_000913/4480802‑4480950                                                                        |                                                                              caAAAAATACGTATTTGCTGGGCGATTTGGATGACCTTCCTTTAATCAATAAAGAAGAACAACCAGATAAAAGCTTCTTAg                                                                      <  1:899983/81‑1 (MQ=255)          cGTATTTGCTGGGCGATTTGGATGACCTTCCTTTAATCAATAAAGAAGAACAACCAGATAAAAGCTTCTTAGCCAGTAttt                                                             <  2:899985/81‑1 (MQ=255)          cGTATTTGCTGGGCGATTTGGATGACCTTCCTTTAATCAATAAAGAAGAACAACCAGATAAAAGCTTCTTAGCCAGTAtt                                                              <  1:899984/80‑1 (MQ=255)           gTATTTGCTGGGCGATTTGGATGACCTTCCTTTAATCAATAAAGAAGAACAACCAGATAAAAGCTTCTTAGCCAGTAttt                                                             >  1:900003/1‑80 (MQ=255)                tGCTGGGCGATTTGGATGACCTTCCTTTAATCAATAAAGAAGAACAACCAGATAAAAGCTTCTTAGCCAGTATTTCACggg                                                       >  2:900014/1‑81 (MQ=255)                 gCTGGGCGATTTGGATGACCTTCCTTTAATCAATAAAGAAGAACAACCAGATAAAAGCTTCTTAGCCAGTATTTCACggg                                                       >  2:900020/1‑80 (MQ=255)                   tGGGCGATTTGGATGACCTTCCTTTAATCAATAAAGAAGAACAACCAGATAAAAGCTTCTTAGCCAGTATTTCACGGGGAg                                                    >  2:900016/1‑81 (MQ=255)                     ggCGATTTGGATGACCTTCCTTTAATCAATAAAGAAGAACAACCAGATAAAAGCTTCTTAGCCAGTATTTCACGGGGAGtt                                                  <  1:899986/81‑1 (MQ=255)                     ggCGATTTGGATGACCTTCCTTTAATCAATAAAGAAGAACAACCAGATAAAAGCTTCTTAGCCAGTATTTCACGGGGAGt                                                   <  1:899987/80‑1 (MQ=255)                      gCGATTTGGATGACCTTCCTTTAATCAATAAAGAAGAACAACCAGATAAAAGCTTCTTAGCCAGTATTTCACGGGGAGTTa                                                 >  1:900027/1‑81 (MQ=255)                      gCGATTTGGATGACCTTCCTTTAATCAATAAAGAAGAACAACCAGATAAAAGCTTCTTAGCCAGTATTTCACGGGGAGTTa                                                 >  2:900011/1‑81 (MQ=255)                      gCGATTTGGATGACCTTCCTTTAATCAATAAAGAAGAACAACCAGATAAAAGCTTCTTAGCCAGTATTTCACGGGGAGTTa                                                 >  2:900005/1‑81 (MQ=255)                            tGGATGACCTTCCTTTAATCAATAAAGAAGAACAACCAGATAAAAGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCctt                                           >  1:900013/1‑81 (MQ=255)                             ggATGACCTTCCTTTAATCAATAAAGAAGAACAACCAGATAAAAGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCcttc                                          >  1:900031/1‑81 (MQ=255)                                    cttccttTAATCAATAAAGAAGAACAACCAGATAAAAGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCa                                   >  2:900021/1‑81 (MQ=255)                                      tccttTAATCAATAAAGAAGAACAACCAGATAAAAGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCAtt                                 >  1:900028/1‑81 (MQ=255)                                           tAATCAATAAAGAAGAACAACCAGATAAAAGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCt                              <  2:899988/79‑1 (MQ=255)                                             aTCAATAAAGAAGAACAACCAGATAAAAGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGt                            >  1:900026/1‑79 (MQ=255)                                              tCAATAAAGAAGAACAACCAGATAAAAGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGt                            >  1:900015/1‑78 (MQ=255)                                                  tAAAGAAGAACAACCAGATAAAAGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAt                     <  2:899989/81‑1 (MQ=255)                                                   aaaGAAGAACAACCAGATAAAAGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAt                     >  2:900025/1‑80 (MQ=255)                                                   aaaGAAGAACAACCAGATAAAAGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAt                     >  2:900009/1‑80 (MQ=255)                                                   aaaGAAGAACAACCAGATAAAAGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAt                     >  1:900012/1‑80 (MQ=255)                                                     agaagaACAACCAGATAAAAGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGATaaa                  <  1:899990/81‑1 (MQ=255)                                                      gaagaaCAACCAGATAAAAGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGATaaaa                 <  1:899991/81‑1 (MQ=255)                                                        agaaCAACCAGATAAAAGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGATaaaa                 >  1:900017/1‑79 (MQ=255)                                                          aacaacCAGATAAAAGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGATAAAAAAgg             >  1:900023/1‑81 (MQ=255)                                                                cAGATAAAAGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGATAAAAAAGGCCGGAg       >  2:900033/1‑81 (MQ=255)                                                                  gATAAAAGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGATAAAAAAGGCCGGAGCa     >  1:900019/1‑81 (MQ=255)                                                                     aaaaGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGATAAAAAAGGCCGGAGCATGc  >  1:900024/1‑81 (MQ=255)                                                                        |                                                                              CAAAAAATACGTATTTGCTGGGCGATTTGGATGACCTTCCTTTAATCAATAAAGAAGAACAACCAGATAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGATAAAAAAGGCCGGAGCATGC  >  NC_000913/4480802‑4480950

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑