| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 4,504,097 | C→T | R14W (CGG→TGG) | yjhB → | putative sialic acid transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,504,097 | 0 | C | T | 96.0% | 84.5 / ‑3.3 | 25 | coding (40/1218 nt) | yjhB | putative sialic acid transporter |
| Reads supporting (aligned to +/- strand): ref base C (0/0); major base T (18/6); minor base . (0/1); total (18/7) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.80e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ACTTTAAGGGCTATATTAGAATAACACAGGAAACAAATATGGCAACAGCATGGTATAAACAAGTTAATCCACCACAACGGAAAGCTCTTTTTTCCGCATGGCTTGGATATGTATTTGATGGCTTTGATTTTATGATGATATTTTACATTCT > NC_000913/4504020‑4504170 | aCTTTAAGGGCTATATTAGAATAACACAGGAAACAAATATGGCAACAGCATGGTATAAACAAGTTAATCCACCACAATgg < 2:905327/80‑1 (MQ=255)aCTTTAAGGGCTATATTAGAATAACACAGGAAACAAATATGGCAACAGCATGGTATAAACAAGTTAATCCACCACAATgg > 2:905360/1‑80 (MQ=255) aaGGGCTATATTAGAATAACACAGGAAACAAATATGGCAACAGCATGGTATAAACAAGTTAATCCACCACAATGGAAAGct > 2:905361/1‑81 (MQ=255) aaGGGCTATATTAGAATAACACAGGAAACAAATATGGCAACAGCATGGTATAAACAAGTTAATCCACCACAATGGAAAGc < 2:905328/80‑1 (MQ=255) ggCTATATTAGAATAACACAGGAAACAAATATGGCAACAGCATGGTATAAACAAGTTAATCCACCACAATGGAAAGCTCtt < 2:905329/81‑1 (MQ=255) atTAGAATAACACAGGAAACAAATATGGCAACAGCATGGTATAAACAAGTTAATCCACCACAATGGAAAGCTCTTTTTTcc > 2:905357/1‑81 (MQ=255) gAATAACACAGGAAACAAATATGGCAACAGCATGGTATAAACAAGTTAATCCACCACAATGGAAAGCTCTTTTTTCCGCAt > 2:905365/1‑81 (MQ=255) acacAGGAAACAAATATGGCAACAGCATGGTATAAACAAGTTAATCCACCACAATGGAAAGCTCTTTTTTCCGCATGGCt < 1:905330/80‑1 (MQ=255) ggAAACAAATATGGCAACAGCATGGTATAAACAAGTTAATCCACCACAATGGAAAGCTCTTTTTTCCGCATGGCTTGGata > 2:905352/1‑81 (MQ=255) gAAACAAATATGGCAACAGCATGGTATAAACAAGTTAATCCACCACAATGGAAAGCTCTTTTTTCCGCATGGCTTGGatat < 2:905331/81‑1 (MQ=255) gAAACAAATATGGCAACAGCATGGTATAAACAAGTTAATCCACCACAATGGAAAGCTCTTTTTTCCGCATGGCTTGGatat > 2:905353/1‑81 (MQ=255) tGGCAACAGCATGGTATAAACAAGTTAATCCACCACAATGGAAAGCTCTTTTTTCCGCATGGCTTGGATATGTATTTGATg > 2:905356/1‑81 (MQ=255) gCAACAGCATGGTATAAACAAGTTAATCCACCACAATGGAAAGCTCTTTTTTCCGCATGGCTTGGATATGTATTTGATGGc > 2:905351/1‑81 (MQ=255) cAACAGCATGGTATAAACAAGTTAATCCACCACAATGGAAAGCTCTTTTTTCCGCATGGCTTGGATATGTATTTGATGGCt > 2:905366/1‑81 (MQ=255) aGCATGGTATAAACAAGTTAATCCACCACAATGGAAAGCTCTTTTTTCCGCATGGCTTGGATATGTATTTGATGGCTTTGa > 1:905354/1‑81 (MQ=255) aTGGTATAAACAAGTTAATCCACCACAATGGAAAGCTCTTTTTTCCGCATGGCTTGGATATGTATTTGATGGCTTTGAttt < 2:905332/81‑1 (MQ=255) ggTATAAACAAGTTAATCCACCACAATGGAAAGCTCTTTTTTCCGCATGGCTTGGATATGTATTTGATGGCTTTGATTTTa > 1:905374/1‑81 (MQ=255) gTATAAACAAGTTAATCCACCACAATGGAAAGCTCTTTTTTCCGCATGGCTTGGATATGTATTTGATGGCTTTGATTTTat > 2:905358/1‑81 (MQ=255) aaaCAAGTTAATCCACCACAATGGAAAGCTCTTTTTTCCGCATGGCTTGGATATGTATTTGATGGCTTTGATTTTatgat > 2:905368/1‑80 (MQ=255) aaCAAGTTAATCCACCACAATGGAAAGCTCTTTTTTCCGCATGGCTTGGATATGTATTTGATGGCTTTGATTTTAtgatga > 1:905373/1‑81 (MQ=255) aCAAGTTAATCCACCACAATGGAAAGCTCTTTTTTCCGCATGGCTTGGATATGTATTTGATGGCTTTGATTTTAtgatga > 1:905363/1‑80 (MQ=255) tAATCCACCACAATGGAAAGCTCTTTTTTCCGCATGGCTTGGATATGTATTTGATGGCTTTGATTTTATGATGATATTTTa > 1:905383/1‑81 (MQ=255) aTCCACCACAATGGAAAGCTCTTTTTTCCGCATGGCTTGGATATGTATTTGATGGCTTTGATTTTATGATGATATTTTACa > 2:905367/1‑81 (MQ=255) caccacAATGGAAAGCTCTTTTTTCCGCATGGCTTGGATATGTATTTGATGGCTTTGATTTTATGATGATATTTTACAttc > 1:905372/1‑81 (MQ=255) caccacAA‑TGAAAGCTCTTTTTTCCGCATGGCTTGGATATGTATTTGATGGCTTTGATTTTATGATGATATTTTACAttct < 2:905333/81‑1 (MQ=255) | ACTTTAAGGGCTATATTAGAATAACACAGGAAACAAATATGGCAACAGCATGGTATAAACAAGTTAATCCACCACAACGGAAAGCTCTTTTTTCCGCATGGCTTGGATATGTATTTGATGGCTTTGATTTTATGATGATATTTTACATTCT > NC_000913/4504020‑4504170 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |