Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,545,445	T→C	M117T (ATG→ACG)	fimD →	type I fimbriae usher protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,545,445	0	T	C	100.0%	92.7 / NA	26	M117T (ATG→ACG)	fimD	type I fimbriae usher protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (15/11);  total (15/11)

TGATGTCACATTTAATACGGGCGACAGTGAACAAGGGATTGTTCCCTGCCTGACACGCGCGCAACTCGCCAGTATGGGGCTGAATACGGCTTCTGTCGCCGGTATGAATCTGCTGGCGGATGATGCCTGTGTGCCATTAACCACAATGGTC  >  NC_000913/4545371‑4545521                                                                           |                                                                             tGATGTCACATTTAATACGGGCGACAGTGAACAAGGGATTGTTCCCTGCCTGACACGCGCGCAACTCGCCAGTACGGGGCt                                                                        <  1:914193/81‑1 (MQ=255)         caTTTAATACGGGCGACAGTGAACAAGGGATTGTTCCCTGCCTGACACGCGCGCAACTCGCCAGTACGGGGCTGAATACgg                                                                <  1:914194/81‑1 (MQ=255)             tAATACGGGCGACAGTGAACAAGGGCTTGTTCCCTGCCTGACACGCGCGCAACTCGCCAGTACGGGGCTGAATACGGCTTc                                                            >  2:914245/1‑81 (MQ=255)              aaTACGGGCGACAGTGAACAAGGGATTGTTCCCTGCCTGACACGCGCGCAACTCGCCAGTACGGGGCTGAATACGGCTTCt                                                           >  1:914231/1‑81 (MQ=255)                   gggCGACAGTGAACAAGGGATTGTTCCCTGCCTGACACGCGCGCAACTCGCCAGTACGGGGCTGAATACGGCTTCTGTCGc                                                      >  1:914238/1‑81 (MQ=255)                    ggCGACAGTGAACAAGGGATTGTTCCCTGCCTGACACGCGCGCAACTCGCCAGTACGGGGCTGAATACGGCTTCTGTCGcc                                                     <  2:914195/81‑1 (MQ=255)                              aaCAAGGGATTGTTCCCTGCCTGACACGCGCGCAACTCGCCAGTACGGGGCTGAATACGGCTTCTGTCGCCGGTATGAATc                                           >  2:914235/1‑81 (MQ=255)                                 aaGGGATTGTTCCCTGCCTGACACGCGCGCAACTCGCCAGTACGGGGCTGAATACGGCTTCTGTCGCCGGTATGAATctgc                                        >  2:914243/1‑81 (MQ=255)                                 aaGGGATTGTTCCCTGCCTGACACGCGCGCAACTCGCCAGTACGGGGCTGAATACGGCTTCTGTCGCCGGTATGAATctgc                                        >  1:914237/1‑81 (MQ=255)                                  aGGGATTGTTCCCTGCCTGACACGCGCGCAACTCGCCAGTACGGGGCTGAATACGGCTTCTGTCGCCGGTATGAATctgct                                       >  2:914252/1‑81 (MQ=255)                                   gggATTGTTCCCTGCCTGACACGCGCGCAACTCGCCAGTACGGGGCTGAATACGGCTTCTGTCGCCGGTATGAATctgctg                                      >  1:914246/1‑81 (MQ=255)                                       ttGTTCCCTGCCTGACACGCGCGCAACTCGCCAGTACGGGGCTGAATACGGCTTCTGTCGCCGGTATGAATCTGCTGGCgg                                  <  1:914196/81‑1 (MQ=255)                                            cccTGCCTGACACGCGCGCAACTCGCCAGTACGGGGCTGAATACGGCTTCTGTCGCCGGTATGAATTTGCTGGCGgatgat                             >  1:914255/1‑81 (MQ=255)                                                 cctgACACGCGCGCAACTCGCCAGTACGGGGCTGAATACGGCTTCTGTCGCCGGTATGAATCTGCTGGCGGATGATGcc                          <  1:914197/79‑1 (MQ=255)                                                     acacGCGCGCAACTCGCCAGTACGGGGCTGAATACGGCTTCTGTCGCCGGTATGAATCTGCTGGCGGATGATGCCtgtgtg                    <  2:914198/81‑1 (MQ=255)                                                         gcgcgcAACTCGCCAGTACGGGGCTGAATACGGCTTCTGTCGCCGGTATGAATCTGCTGGCGGATGATGCCTGTGTGCCAt                >  2:914259/1‑81 (MQ=255)                                                             gcAACTCGCCAGTACGGGGCTGAATACGGCTTCTGTCGCCGGTATGAATCTGCTGGCGGATGATGCCTGTGTGCCATTAAc            >  1:914263/1‑81 (MQ=255)                                                             gcAACTCGCCAGTACGGGGCTGAATACGGCTTCTGTCGCCGGTATGAATCTGCTGGCGGATGATGCCTGTGTGCCATTAAc            >  2:914254/1‑81 (MQ=255)                                                              cAACTCGCCAGTACGGGGCTGAATACGGCTTCTGTCGCCGGTATGAATCTGCTGGCGGATGATGCCTGTGTGCCATTAAcc           <  2:914199/81‑1 (MQ=255)                                                               aaCTCGCCAGTACGGGGCTGAATACGGCTTCTGTCGCCGGTATGAATCTGCTGGCGGATGATGCCTGTGTGCCATTAACca          >  1:914256/1‑81 (MQ=255)                                                               aaCTCGCCAGTACGGGGCTGAATACGGCTTCTGTCGCCGGTATGAATCTGCTGGCGGATGATGCCTGTGTGCCATTAACca          <  1:914200/81‑1 (MQ=255)                                                                    gCCAGTACGGGGCTGAATACGGCTTCTGTCGCCGGTATGAATCTGCTGGCGGATGATGCCTGTGTGCCATTAACCACAATg     >  2:914249/1‑81 (MQ=255)                                                                     ccAGTACGGGGCTGAATACGGCTTCTGTCGCCGGTATGAATCTGCTGGCGGATGATGCCTGTGTGCCATTAACCACAATgg    >  1:914247/1‑81 (MQ=255)                                                                     ccAGTACGGGGCTGAATACGGCTTCTGTCGCCGGTATGAATCTGCTGGCGGATGATGCCTGTGTGCCATTAACCACAATgg    <  1:914201/81‑1 (MQ=255)                                                                      cAGTACGGGGCTGAATACGGCTTCTGTCGCCGGTATGAATCTGCTGGCGGATGATGCCTGTGTGCCATTAACCACAATGGt   <  1:914202/81‑1 (MQ=255)                                                                       aGTACGGGGCTGAATACGGCTTCTGTCGCCGGTATGAATCTGCTGGCGGATGATGCCTGTGTGCCATTAACCACAATGGTc  <  1:914203/81‑1 (MQ=255)                                                                           |                                                                             TGATGTCACATTTAATACGGGCGACAGTGAACAAGGGATTGTTCCCTGCCTGACACGCGCGCAACTCGCCAGTATGGGGCTGAATACGGCTTCTGTCGCCGGTATGAATCTGCTGGCGGATGATGCCTGTGTGCCATTAACCACAATGGTC  >  NC_000913/4545371‑4545521

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑