Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	447,488	T→C	N73S (AAT→AGT)	cyoE ←	heme O synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	447,488	0	T	C	100.0%	122.3 / NA	34	N73S (AAT→AGT)	cyoE	heme O synthase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (14/20);  total (14/20)

ATAAAGCCAGCAATACCCAGCAACGTGGCGTACACCAGCGAGACAGCAGGAGAGATCAGGCCTTTCACCAGCACCCGATTCTTCGTCCTTTCCATCTTTCTGTCGATATCCCTGTCGATGTAGTTGTTAAACACACAACCCGACGCCACAACCAG  >  NC_000913/447410‑447564                                                                               |                                                                             aTAAAGCCAGCAATACCCAGCAACGTGGCGTACACCAGCGAGACAGCAGGAGAGATCAGGCCTTTCACCAGCACCCGActc                                                                            <  1:95147/81‑1 (MQ=255) aTAAAGCCAGCAATACCCAGCAACGTGGCGTACACCAGCGAGACAGCAGGAGAGATCAGGCCTTTCACCAGCACCCGActc                                                                            >  2:95200/1‑81 (MQ=255)  tAAAGCCAGCAATACCCAGCAACGTGGCGTACACCAGCGAGACAGCAGGAGAGATCAGGCCTTTCACCAGCACCCGActct                                                                           <  2:95148/81‑1 (MQ=255)    aaGCCAGCAATACCCAGCAACGTGGCGTACACCAGCGAGACAGCAGGAGAGATCAGGCCTTTCACCAGCACCCGActcttc                                                                         <  1:95149/81‑1 (MQ=255)       ccAGCAATACCCAGCAACGTGGCGTACACCAGCGAGACAGCAGGAGAGATCAGGCCTTTCACCAGCACCCGACTCTTCGTc                                                                      <  2:95150/81‑1 (MQ=255)        cAGCAATACCCAGCAACGTGGCGTACACCAGCGAGACAGCAGGAGAGATCAGGCCTTTCACCAGCACCCGACTCTTCGTcc                                                                     >  2:95216/1‑81 (MQ=255)         aGCAATACCCAGCAACGTGGCGTACACCAGCGAGACAGCAGGAGAGATCAGGCCTTTCACCAGCACCCGACTCTTCGTCCt                                                                    <  2:95151/81‑1 (MQ=255)             aTACCCAGCAACGTGGCGTACACCAGCGAGACAGCAGGAGAGATCAGGCCTTTCACCAGCACCCGACTCTTCGTCCTTTc                                                                 >  1:95199/1‑80 (MQ=255)              tACCCAGCAACGTGGCGTACACCAGCGAGACAGCAGGAGAGATCAGGCCTTTCACCAGCACCCGACTCTTCGTCCTTTCCa                                                               <  1:95152/81‑1 (MQ=255)               aCCCAGCAACGTGGCGTACACCAGCGAGACAGCAGGAGAGATCAGGCCTCTCACCAGCACCCGACTCTTCGTCCTTTCCAt                                                              >  2:95202/1‑81 (MQ=255)                cccAGCAACGTGGCGTACACCAGCGAGACAGCAGGAGAGATCAGGCCTTTCACCAGCACCCGACTCTTCGTCCTTTCCAt                                                              <  2:95153/80‑1 (MQ=255)                cccAGCAACGTGGCGTACACCAGCGAGACAGCAGGAGAGATCAGGCCTTTCACCAGCACCCGACTCTTCGTCCTTTCCATc                                                             >  2:95211/1‑81 (MQ=255)                     cAACGTGGCGTACACCAGCGAGACAGCAGGAGAGATCAGGCCTTTCACCAGCACCCGACTCTTCGTCCTTTCCATCTTTCt                                                        <  1:95155/81‑1 (MQ=255)                     cAACGTGGCGTACACCAGCGAGACAGCAGGAGAGATCAGGCCTTTCACCAGCACCCGACTCTTCGTCCTTTCCATCTTTCt                                                        <  1:95154/81‑1 (MQ=255)                         gTGGCGTACACCAGCGAGACAGCAGGAGAGATCAGGCCTTTCACCAGCACCCGACTCTTCGTCCTTTCCATCTTTCTGTc                                                     >  2:95212/1‑80 (MQ=255)                          tGGCGTACACCAGCGAGACAGCAGGAGAGATCAGGCCTTTCACCAGCACCCGACTCTTCGTCCTTTCCATCTTTCTGTCGa                                                   <  1:95156/81‑1 (MQ=255)                            gCGTACACCAGCGAGACAGCAGGAGAGATCAGGCCTTTCACCAGCACCCGACTCTTCGTCCTTTCCATCTTTCTGTCGat                                                  <  1:95157/80‑1 (MQ=255)                              gTACACCAGCGAGACAGCAGGAGAGATCAGGCCTTTCACCAGCACCCGACTCTTCGTCCTTTCCATCTTTCTGTCGATATc                                               >  2:95205/1‑81 (MQ=255)                               tACACCAGCGAGACAGCAGGAGAGATCAGGCCTTTCACCAGCACCCGACTCTTCGTCCTTTCCATCTTTCTGTCGATATcc                                              <  2:95158/81‑1 (MQ=255)                                acacCAGCGAGACAGCAGGAGAGATCAGGCCTTTCACCAGCACCCGACTCTTCGTCCTTTCCATCTTTCTGTCGATATccc                                             >  2:95214/1‑81 (MQ=255)                                    cAGCGAGACAGCAGGAGAGATCAGGCCTTTCACCAGCACCCGACTCTTCGTCCTTTCCATCTTTCTGTCGATATCCCTGTc                                         <  1:95160/81‑1 (MQ=255)                                    cAGCGAGACAGCAGGAGAGATCAGGCCTTTCACCAGCACCCGACTCTTCGTCCTTTCCATCTTTCTGTCGATATCCCTGTc                                         <  2:95159/81‑1 (MQ=255)                                     aGCGAGACAGCAGGAGAGATCAGGCCTTTCACCAGCACCCGACTCTTCGTCCTTTCCATCTTTCTGTCGATATCCCTGTc                                         >  2:95196/1‑80 (MQ=255)                                     aGCGAGACAGCAGGAGAGATCAGGCCTTTCACCAGCACCCGACTCTTCGTCCTTTCCATCTTTCTGTCGATATCCCTGTCg                                        <  2:95161/81‑1 (MQ=255)                                                   agagaTCAGGCCTTTCACCAGCACCCGACTCTTCGTCCTTTCCATCTTTTTGTCGATATCCCTGTCGATGTAGTTGTTaaa                          >  2:95209/1‑81 (MQ=255)                                                      gaTCAGGCCTTTCACCAGCACCCGACTCTTCGTCCTTTCCATCTTTCTGTCGATATCCCTGTCGATGTAGTTGTTAAaca                        >  2:95227/1‑80 (MQ=255)                                                       aTCAGGCCTTTCACCAGCACCCGACTCTTCGTCCTTTCCATCTTTCTGTCGATATCCCTGTCGATGTAGTTGTTAAacaca                      >  1:95218/1‑81 (MQ=255)                                                          aGGCCTTTCACCAGCACCCGACTCTTCGTCCTTTCCATCTTTCTGTCGATATCCCTGTCGATGTAGTTGTTAAACACACaa                   <  1:95162/81‑1 (MQ=255)                                                          aGGCCTTTCACCAGCACCCGACTCTTCGTCCTTTCCATCTTTCTGTCGATATCCCTGTCGATGTAGTTGTTAAACACACaa                   >  1:95208/1‑81 (MQ=255)                                                              cTTTCACCAGCACCCGACTCTTCGTCCTTTCCATCTTTCTGTCGATATCCCTGTCGATGTAGTTGTTAAACACACAAccc                >  1:95213/1‑80 (MQ=255)                                                              cTTTCACCAGCACCCGACTCTTCGTCCTTTCCATCTTTCTGTCGATATCCCTGTCGATGTAGTTGTTAAACACACAACCCg               <  1:95163/81‑1 (MQ=255)                                                                  cACCAGCACCCGACTCTTCGTCCTTTCCATCTTTCTGTCGATATCCCTGTCGATGTAGTTGTTAAACACACAACCCGACg            <  1:95164/80‑1 (MQ=255)                                                                  cACCAGCACCCGACTCTTCGTCCTTTCCATCTTTCTGTCGATATCCCTGTCGATGTAGTTGTTAAACACACAACCCGACGc           >  2:95207/1‑81 (MQ=255)                                                                   aCCAGCACCCGACTCTTCGTCCTTTCCATCTTTCTGTCGATATCCCTGTCGATGTAGTTGTTAAACACACAACCCGACGcc          <  1:95165/81‑1 (MQ=255)                                                                   aCCAGCACCCGACTCTTCGTCCTTTCCATCTTTCTGTCGATATCCCTGTCGATGTAGTTGTTAAACACACAACCCGACGc           <  2:95167/80‑1 (MQ=255)                                                                   aCCAGCACCCGACTCTTCGTCCTTTCCATCTTTCTGTCGATATCCCTGTCGATGTAGTTGTTAAACACACAACCCGACGc           <  1:95166/80‑1 (MQ=255)                                                                          cccGACTCTTCGTCCTTTCCATCTTTCTGTCGATATCCCTGTCGATGTAGTTGTTAAACACACAACCCGACGCCACAACCa   <  1:95168/81‑1 (MQ=255)                                                                           ccGACTCTTCGTCCTTTCCATCTTTCTGTCGATATCCCTGTCGATGTAGTTGTTAAACACACAACCCGACGCCACAACCAg  <  1:95169/81‑1 (MQ=255)                                                                               |                                                                             ATAAAGCCAGCAATACCCAGCAACGTGGCGTACACCAGCGAGACAGCAGGAGAGATCAGGCCTTTCACCAGCACCCGATTCTTCGTCCTTTCCATCTTTCTGTCGATATCCCTGTCGATGTAGTTGTTAAACACACAACCCGACGCCACAACCAG  >  NC_000913/447410‑447564

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑