Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,559,565	T→A	I146F (ATC→TTC)	yjiG ←	Gate family protein YjiG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,559,565	0	T	A	100.0%	113.3 / NA	32	I146F (ATC→TTC)	yjiG	Gate family protein YjiG
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (14/18);  total (14/18)

TGCAGCAGGGTAAAACCGGCTGCGGTATAATCAATCATGGTAACTCCTTGTTAAACAATAAGCTGCATAACCCAGATCGACAGCAATGCGTTAATCACGCAGACCGTAATGATATGCGGGTAATATTTGGCATTCACTTCGGCTGTCCCCAGG  >  NC_000913/4559489‑4559641                                                                             |                                                                             tGCAGCAGGGTAAAACCGGCTGCGGTATAATCAATCATGGTAACTCCTTGTTAAACAATAAGCTGCATAACCCAGAACGa                                                                           >  2:917423/1‑80 (MQ=255)   cagcagGGTAAAACCGGCTGCGGTATAATCAATCATGGTAACTCCTTGTTAAACAATAAGCTGCATAACCCAGAACGAc                                                                          <  2:917395/79‑1 (MQ=255)   cagcagGGTAAAACCGGCTGCGGTATAATCAATCATGGTAACTCCTTGTTAAACAATAAGCTGCATAACCCAGAACGACa                                                                         <  2:917393/80‑1 (MQ=255)   cagcagGGTAAAACCGGCTGCGGTATAATCAATCATGGTAACTCCTTGTTAAACAATAAGCTGCATAACCCAGAACGACAg                                                                        <  1:917394/81‑1 (MQ=255)   cagcagGGTAAAACCGGCTGCGGTATAATCAATCATGGTAACTCCTTGTTAAACAATAAGCTGCATAACCCAGAACGACAg                                                                        >  2:917437/1‑81 (MQ=255)   cagcagGGTAAAACCGGCTGCGGTATAATCAATCATGGTAACTCCTTGTTAAACAATAAGCTGCATAACCCAGAACGACAg                                                                        >  1:917430/1‑81 (MQ=255)       agGGTAAAACCGGCTGCGGTATAATCAATCATGGTAACTCCTTGTTAAACAATAAGCTGCATAACCCAGAACGACAGCAAt                                                                    >  1:917422/1‑81 (MQ=255)        gggTAAAACCGGCTGCGGTATAATCAATCATGGTAACTCCTTGTTAAACAATAAGCTGCATAACCCAGAACGACAGCAATg                                                                   >  2:917416/1‑81 (MQ=255)             aaaCCGGCTGCGGTATAATCAATCATGGTAACTCCTTGTTAAACAATAAGCTGCATAACCCAGAACGACAGCAATGCGTTa                                                              <  2:917396/81‑1 (MQ=255)              aaCCGGCTGCGGTATAATCAATCATGGTAACTCCTTGTTAAACAATAAGCTGCATAACCCAGAACGACAGCAATGCGTTaa                                                             >  1:917429/1‑81 (MQ=255)                  ggCTGCGGTATAATCAATCATGGTAACTCCTTGTTAAACAATAAGCTGCATAACCCAGAACGACAGCAATGCGTTAATCAc                                                         <  2:917397/81‑1 (MQ=255)                    cTGCGGTATAATCAATCATGGTAACTCCTTGTTAAACAATAAGCTGCATAACCCAGAACGACAGCAATGCGTTAATCACg                                                        >  2:917435/1‑80 (MQ=255)                    cTGCGGTATAATCAATCATGGTAACTCCTTGTTAAACAATAAGCTGCATAACCCAGAACGACAGCAATGCGTTAATCACGc                                                       <  1:917398/81‑1 (MQ=255)                      gCGGTATAATCAATCATGGTAACTCCTTGTTAAACAATAAGCTGCATAACCCAGAACGACAGCAATGCGTTAATCACg                                                        >  2:917441/1‑78 (MQ=255)                            taATCAATCATGGTAACTCCTTGTTAAACAATAAGCTGCATAACCCAGAACGACAGCAATGCGTTAATCACGCAGACCGt                                                >  1:917431/1‑80 (MQ=255)                            taATCAATCATGGTAACTCCTTGTTAAACAATAAGCTGCATAACCCAGAACGACAGCAATGCGTTAATCACGCAGACCGt                                                <  1:917399/80‑1 (MQ=255)                              atcaatcaTGGTAACTCCTTGTTAAACAATAAGCTGCATAACCCAGAACGACAGCAATGCGTTAATCACGCAGACCGTaa                                              <  2:917400/80‑1 (MQ=255)                                  atcaTGGTAACTCCTTGTTAAACAATAAGCTGCATAACCCAGAACGACAGCAATGCGTTAATCACGCAGACCGTAATGata                                         <  1:917401/81‑1 (MQ=255)                                   tcaTGGTAACTCCTTGTTAAACAATAAGCTGCATAACCCAGAACGACAGCAATGCGTTAATCACGCAGACCGTAAta                                            <  2:917402/77‑2 (MQ=255)                                        gTAACTTCTTGTTAAACAATAAGCTGCATAACCCAGAACGACAGCAATGCGTTAATCACGCAGACCGTAATGATATGCggg                                   >  1:917450/1‑81 (MQ=255)                                           aCTCCTTGTTAAACAATAAGCTGCATAACCCAGAACGACAGCAATGCGTTAATCACGCAGACCGTAATGATATGCGGGTa                                 <  2:917403/80‑1 (MQ=255)                                                      aaCAATAAGCTGCATAACCCAGAACGACAGCAATGCGTTAATCACGCAGACCGTAATGATATGCGGGTAATATTTGGCAtt                     <  1:917404/81‑1 (MQ=255)                                                          aTAAGCTGCATAACCCAGAACGACAGCAATGCGTTAATCACGCAGACCGTAATGATATGCGGGTAATATTTGGCATTCACt                 >  2:917452/1‑81 (MQ=255)                                                           tAAGCTGCATAACCCAGAACGACAGCAATGCGTTAATCACGCAGACCGTAATGATATGCGGGTAATATTTGGCATTCACtt                <  2:917405/81‑1 (MQ=255)                                                            aaGCTGCATAACCCAGAACGACAGCAATGCGTTAATCACGCAGACCGTAATGATATGCGGGTAATATTTGGCATTCACTTc               >  2:917434/1‑81 (MQ=255)                                                             aGCTGCATAACCCAGAACGACAGCAATGCGTTAATCACGCAGACCGTAATGATATGCGGGTAATATTTGGCATTCACTTc               <  2:917406/80‑1 (MQ=255)                                                                  cATAACCCAGAACGACAGCAATGCGTTAATCACGCAGACCGTAATGATATGCGGGTAATATTTGGCATTCACTTCGGCt           <  1:917407/79‑1 (MQ=255)                                                                    tAACCCAGAACGACAGCAATGCGTTAATCACGCAGACCGTAATGATATGCGGGTAATATTTGGCATTCACTTCGGCTGTcc       <  1:917408/81‑1 (MQ=255)                                                                      aCCCAGAACGACAGCAATGCGTTAATCACGCAGACCGTAATGATATGCGGGTAATATTTGGCATTCACTTCGGCTGTcccc     <  2:917409/81‑1 (MQ=255)                                                                       cccAGAACGACAGCAATGCGTTAATCACGCAGACCGTAATGATATGCGGGTAATATTTGGCATTCACTTCGGCTGTCCCCa    <  1:917410/81‑1 (MQ=255)                                                                          aGAACGACAGCAATGCGTTAATCACGCAGACCGTAATGATATGCGGGTAATATTTGGCATTCACTTCGGCTGTCCCCAgg  >  2:917436/1‑80 (MQ=255)                                                                          aGAACGACAGCAATGCGTTAATCACGCAGACCGTAATGATATGCGGGTAATATTTGGCATTCACTTCGGCTGTCCCCAgg  >  1:917445/1‑80 (MQ=255)                                                                             |                                                                             TGCAGCAGGGTAAAACCGGCTGCGGTATAATCAATCATGGTAACTCCTTGTTAAACAATAAGCTGCATAACCCAGATCGACAGCAATGCGTTAATCACGCAGACCGTAATGATATGCGGGTAATATTTGGCATTCACTTCGGCTGTCCCCAGG  >  NC_000913/4559489‑4559641

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑