Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,559,878	C→T	A41A (GCG→GCA)	yjiG ←	Gate family protein YjiG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,559,878	0	C	T	100.0%	138.9 / NA	38	A41A (GCG→GCA)	yjiG	Gate family protein YjiG
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (20/18);  total (20/18)

CACCGGGTAATCCCCACAGCGCCATTACGGGTTCACAGATATGCCCCACCCAGTCGAGCAGGCCGGTAATTTTCAGCGCCTGAATGATGACAAACGCCATCACCACGTTTGGCAGCAGGTTGGTCGTGGCGATAGTAAAGCCACGGCGCGCGC  >  NC_000913/4559802‑4559954                                                                             |                                                                             cACCGGGTAATCCCCACAGCGCCATTACGGGTTCACAGATATGCCCCACCCAGTCGAGCAGGCCGGTAATTTTCAgtgc                                                                            <  1:917446/79‑1 (MQ=255) cACCGGGTAATCCCCACAGCGCCATTACGGGTTCACAGATATGCCCCACCCAGTCGAGCAGGCCGGTAATTTTCAGTGCCt                                                                          >  2:917489/1‑81 (MQ=255)  aCCGGGTAATCCCCACAGCGCCATTACGGGTTCACAGATATGCCCCACCCAGTCGAGCAGGCCGGTAATTTTCAGTGCCt                                                                          <  1:917447/80‑1 (MQ=255)    cGGGTAATCCCCACAGCGCCATTACGGGTTCACAGATATGCCCCACCCAGTCGAGCAGGCCGGTAATTTTCAGTGCCTGaa                                                                       >  1:917504/1‑81 (MQ=255)        tAATCCCCACAGCGCCATTACGGGTTCACAGATATGCCCCACCCAGTCGAGCAGGCCGGTAATTTTCAGTGCCTGAatgat                                                                   >  1:917500/1‑81 (MQ=255)             cccACAGCGCCATTACGGGTTCACAGATATGCCCCACCCAGTCGAGCAGGCCGGTAATTTTCAGTGCCTGAATGATGACaa                                                              <  1:917448/81‑1 (MQ=255)               cacaGCGCCATTACGGGTTCACAGATATGCCCCACCCAGTCGAGCAGGCCGGTAATTTTCAGTGCCTGAATGATGACAAAc                                                            <  1:917449/81‑1 (MQ=255)                 caGCGCCATTACGGGTTCACAGATATGCCCCACCCAGTCGAGCAGGCCGGTAATTTTCAGTGCCTGAATGATGACAAACGc                                                          <  2:917450/81‑1 (MQ=255)                  aGCGCCATTACGGGTTCACAGATATGCCCCACCCAGTCGAGCAGGCCGGTAATTTTCAGTGCCTGAATGATGACAAACGc                                                          >  2:917496/1‑80 (MQ=255)                  aGCGCCATTACGGGTTCACAGATATGCCCCACCCAGTCGAGCAGGCCGGTAATTTTCAGTGCCTGAATGATGACAAACGc                                                          >  1:917490/1‑80 (MQ=255)                    cgcCATTACGGGTTCACAGATATGCCCCACCCAGTCGAGCAGGCCGGTAATTTTCAGTGCCTGAATGATGACAAACGCCAt                                                       >  1:917503/1‑81 (MQ=255)                     gcCATTACGGGTTCACAGATATGCCCCACCCAGTCGAGCAGGCCGGTAATTTTCAGTGCCTGAATGATGACAAACGCCAt                                                       >  2:917505/1‑80 (MQ=255)                       cATTACGGGTTCACAGATATGCCCCACCCAGTCGAGCAGGCCGGTAATTTTCAGTGCCTGAATGATGACAAACGCCATcac                                                    <  1:917451/81‑1 (MQ=255)                       cATTACGGGTTCACAGATATGCCCCACCCAGTCGAGCAGGCCGGTAATTTTCAGTGCCTGAATGATGACAAACGCCATcac                                                    <  1:917452/81‑1 (MQ=255)                        aTTACGGGTTCACAGATATGCCCCACCCAGTCGAGCAGGCCGGTAATTTTCAGTGCCTGAATGATGACAAACGCCATcacc                                                   <  2:917453/81‑1 (MQ=255)                        aTTACGGGTTCACAGATATGCCCCACCCAGTCGAGCAGGCCGGTAATTTTCAGTGCCTGAATGATGACAAACGCCATcac                                                    >  2:917509/1‑80 (MQ=255)                           aCGGGTTCACAGATATGCCCCACCCAGTCGAGCAGGCCGGTAATTTTCAGTGCCTGAATGATGACAAACGCCATCACCACg                                                >  1:917454/1‑81 (MQ=255)                              ggTTCACAGATATGCCCCACCCAGTCGAGCAGGCCGGTAATTTTCAGTGCCTGAATGATGACAAACGCCATCACCACGttt                                             <  1:917455/81‑1 (MQ=255)                              ggTTCACAGATATGCCCCACCCAGTCGAGCAGGCCGGTAATTTTCAGTGCCTGAATGATGACAAACGCCATCACCACGttt                                             >  2:917494/1‑81 (MQ=255)                               gTTCACAGATATGCCCCACCCAGTCGAGCAGGCCGGTAATTTTCAGTGCCTGAATGATGACAAACGCCATCACCACGTTTg                                            >  2:917492/1‑81 (MQ=255)                                ttCACAGATATGCCCCACCCAGTCGAGCAGGCCGGTAATTTTCAGTGCCTGAATGATGACAAACGCCATCACCACGTTTgg                                           >  1:917510/1‑81 (MQ=255)                                       atatGCCCCACCCAGTCGAGCAGGCCGGTAATTTTCAGTGCCTGAATGATGACAAACGCCATCACCACGTTTGGCAGCAgg                                    >  2:917495/1‑81 (MQ=255)                                        tatGCCCCACCCAGTCGGGCAGGCCGGTAATTTTCAGTGCCTGAATGATGACAAACGCCATCACCACGTTTGGCAGCAGGt                                   <  2:917456/81‑1 (MQ=255)                                             cccacccaGTCGAGCAGGCCGGTAATTTTCAGTGCCTGAATGATGACAAACGCCATCACCACGTTTGGCAGCAGGTTGGTc                              <  1:917458/81‑1 (MQ=255)                                             cccacccaGTCGAGCAGGCCGGTAATTTTCAGTGCCTGAATGATGACAAACGCCATCACCACGTTTGGCAGCAGGTTGGTc                              <  2:917457/81‑1 (MQ=255)                                                acccaGTCGAGCAGGCCGGTAATTTTCAGTGCCTGAATGATGACAAACGCCATCACCACGTTTGGCAGCAGGTTGGTCGTg                           >  2:917493/1‑81 (MQ=255)                                                acccaGTCGAGCAGGCCGGTAATTTTCAGTGCCTGAATGATGACAAACGCCATCACCACGTTTGGCAGCAGGTTGGTCGTg                           <  1:917459/81‑1 (MQ=255)                                                  ccaGTCGAGCAGGCCGGTAATTTTCAGTGCCTGAATGATGACAAACGCCATCACCACGTTTGGCAGCAGGTTGGTCGTGGc                         <  2:917460/81‑1 (MQ=255)                                                         aGCAGGCCGGTAATTTTCAGTGCCTGAATGATGACAAACGCCATCACCACGTTTGGCAGCAGGTTGGTCGTGGCGATAGt                   >  1:917506/1‑80 (MQ=255)                                                         aGCAGGCCGGTAATTTTCAGTGCCTGAATGATGACAAACGCCATCACCACGTTTGGCAGCAGGTTGGTCGTGGCGATAGTa                  <  2:917461/81‑1 (MQ=255)                                                         aGCAGGCCGGTAATTTTCAGTGCCTGAATGATGACAAACGCCATCACCACGTTTGGCAGCAGGTTGGTCGTGGCGATAGTa                  <  2:917462/81‑1 (MQ=255)                                                           cAGGCCGGTAATTTTCAGTGCCTGAATGATGACAAACGCCATCACCACGTTTGGCAGCAGGTTGGTCGTGGCGATAGTaaa                <  1:917463/81‑1 (MQ=255)                                                            aGGCCGGTAATTTTCAGTGCCTGAATGATGACAAACGCCATCACCACGTTTGGCAGCAGGTTGGTCGTGGCGATAGTAAAg               >  1:917507/1‑81 (MQ=255)                                                               ccGGTAATTTTCAGTGCCTGAATGATGACAAACGCCATCACCACGTTTGGCAGCAGGTTGGTCGTGGCGATAGTAAAGCCa            >  1:917501/1‑81 (MQ=255)                                                                 ggTAATTTTCAGTGCCTGAATGATGACAAACGCCATCACCACGTTTGGCAGCAGGTTGGTCGTGGCGATAGTAAAGCCACg          >  1:917514/1‑81 (MQ=255)                                                                   tAATTTTCAGTGCCTGAATGATGACAAACGCCATCACCACGTTTGGCAGCAGGTTGGTCGTGGCGATAGTAAAGCCACGgc        <  2:917464/81‑1 (MQ=255)                                                                   tAATTTTCAGTGCCTGAATGATGACAAACGCCATCACCACGTTTGGCAGCAGGTTGGTCGTGGCGATAGTAAAGCCACGgc        <  1:917465/81‑1 (MQ=255)                                                                       tttCAGTGCCTGAATGATGACAAACGCCATCACCACGTTTGGCAGCAGGTTGGTCGTGGCGATAGTAAAGCCACGgcgcgc    >  1:917498/1‑81 (MQ=255)                                                                          cAGTGCCTGAATGATGACAAACGCCATCACCACGTTTGGCAGCAGGTTGGTCGTGGCGATAGTAAAGCCACGgcgcgcgc  >  2:917512/1‑80 (MQ=255)                                                                             |                                                                             CACCGGGTAATCCCCACAGCGCCATTACGGGTTCACAGATATGCCCCACCCAGTCGAGCAGGCCGGTAATTTTCAGCGCCTGAATGATGACAAACGCCATCACCACGTTTGGCAGCAGGTTGGTCGTGGCGATAGTAAAGCCACGGCGCGCGC  >  NC_000913/4559802‑4559954

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑