Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,579,647	C→A	G65V (GGC→GTC)	symE ←	toxic protein SymE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,579,647	0	C	A	100.0%	113.4 / NA	32	G65V (GGC→GTC)	symE	toxic protein SymE
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (20/12);  total (20/12)

ACCTGGCGTAGCGACTGCATCAGTTCGCTCTCCTCGGCGGCGGGTGGTTGGGCGGTGAGGACAATACAGCCTTCCATCACTTTGACATCTACCGCCGTGCCAGTGGCAAAACCGGCGGCTTCCAGCCACTGACCTTTCAGGGT  >  NC_000913/4579578‑4579720                                                                      |                                                                          acCTGGCGTAGCGACTGCATCAGTTCGCTCTCCTCGGCGGCGGGTGGTTGGGCGGTGAGGACAATACAGACTTCCATCACt                                                                >  1:921445/1‑81 (MQ=255) acCTGGCGTAGCGACTGCATCAGTTCGCTCTCCTCGGCGGCGGGTGGTTGGGCGGTGAGGACAATACAGACTTCCATCACt                                                                <  2:921386/81‑1 (MQ=255)   cTGGCGTAGCGACTGCATCAGTTCGCTCTCCTCGGCGGCGGGTGGTTGGGCGGTGAGGACAATACAGACTTCCATCACttt                                                              >  2:921426/1‑81 (MQ=255)   cTGGCGTAGCGACTGCATCAGTTCGCTCTCCTCGGCGGCGGGTGGTTGGGCGGTGAGGACAATACAGACTTCCATCACttt                                                              <  2:921387/81‑1 (MQ=255)     ggCGTAGCGACTGCATCAGTTCGCTCTCCTCGGCGGCGGGTGGTTGGGCGGTGAGGACAATACAGACTTCCATCACTTTGa                                                            >  1:921423/1‑81 (MQ=255)      gCGTAGCGACTGCATCAGTTCGCTCTCCTCGGCGGCGGGTGGTTGGGCGGTGAGGACAATACAGACTTCCATCACTTTGAc                                                           <  1:921388/81‑1 (MQ=255)       cGTAGCGACTGCATCAGTTCGCTCTCCTCGGCGGCGGGTGGTTGGGCGGTGAGGACAATACAGACTTCCATCACTTTGACa                                                          >  2:921429/1‑81 (MQ=255)         tAGCGACTGCATCAGTTCGCTCTCCTCGGCGGCGGGTGGTTGGGCGGTGAGGACAATACAGACTTCCATCACTTTGACATc                                                        >  2:921444/1‑81 (MQ=255)          aGCGACTGCATCAGTTCGCTCTCCTCGGCGGCGGGTGGTTGGGCGGTGAGGACAATACAGACTTCCATCACTTTGACATc                                                        >  2:921435/1‑80 (MQ=255)          aGCGACTGCATCAGTTCGCTCTCCTCGGCGGCGGGTGGTTGGGCGGTGAGGACAATACAGACTTCCATCACTTTGACATc                                                        >  2:921441/1‑80 (MQ=255)            cGACTGCATCAGTTCGCTCTCCTCGGCGGCGGGTGGTTGGGCGGTGAGGACAATACAGACTTCCATCACTTTGACATCTAc                                                     <  1:921389/81‑1 (MQ=255)               cTGCATCAGTTCGCTCTCCTCGGCGGCGGGTGGTTGGGCGGTGAGGACAATACAGACTTCCATCACTTTGACATCTAccgc                                                  >  1:921427/1‑81 (MQ=255)                  cATCAGTTCGCTCTCCTCGGCGGCGGGTGGTTGGGCGGTGAGGACAATACAGACTTCCATCACTTTGACATCTAccgccg                                                <  1:921390/80‑1 (MQ=255)                  cATCAGTTCGCTCTCCTCGGCGGCGGGTGGTTGGGCGGTGAGGACAATACAGACTTCCATCACTTTGACATCTACCGCCGt                                               >  2:921438/1‑81 (MQ=255)                  cATCAGTTCGCTCTCCTCGGCGGCGGGTGGTTGGGCGGTGAGGACAATACAGACTTCCATCACTTTGACATCTACCGCCGt                                               <  2:921391/81‑1 (MQ=255)                    tCAGTTCGCTCTCCTCGGCGGCGGGTGGTTGGGCGGTGAGGACAATACAGACTTCCATCACTTTGACATCTACCGCCGTGc                                             <  2:921392/81‑1 (MQ=255)                         tCGCTCTCCTCGGCGGCGGGTGGTTGGGCGGTGAGGACAATACAGACTTCCATCACTTTGACATCTACCGCCGTGCCAGTg                                        >  2:921448/1‑81 (MQ=255)                             tctcCTCGGCGGCGGGTGGTTGGGCGGTGAGGACAATACAGACTTCCATCACTTTGACATCTACCGCCGTGCCAGTGGCa                                     >  2:921419/1‑80 (MQ=255)                                     gcggcggGTGGTTGGGCGGTGAGGACAATACAGACTTCCATCACTTTGACATCTACCGCCGTGCCAGTGGCAAAACcggcg                            >  2:921451/1‑81 (MQ=255)                                       ggcggGTGGTTGGGCGGTGAGGACAATACAGACTTCCATCACTTTGACATCTACCGCCGTGCCAGTGGCAAAACCggcggc                          >  2:921442/1‑81 (MQ=255)                                          gggTGGTTGGGCGGTGAGGACAATACAGACTTCCATCACTTTGACATCTACCGCCGTGCCAGTGGCAAAACCGGCGGCTTc                       >  2:921393/1‑81 (MQ=255)                                           ggtggtTGGGCGGTGAGGACAATACAGACTTCCATCACTTTGACATCTACCGCCGTGCCAGTGGCAAAACCGGCGGCTTcc                      <  2:921396/81‑1 (MQ=255)                                           ggtggtTGGGCGGTGAGGACAATACAGACTTCCATCACTTTGACATCTACCGCCGTGCCAGTGGCAAAACCGGCGGCTTcc                      <  1:921395/81‑1 (MQ=255)                                           ggtggtTGGGCGGTGAGGACAATACAGACTTCCATCACTTTGACATCTACCGCCGTGCCAGTGGCAAAACCGGCGGCTTcc                      <  1:921394/81‑1 (MQ=255)                                               gtTGGGCGGTGAGGACAATACAGACTTCCATCACTTTGACATCTACCGCCGTGCCAGTGGCAAAACCGGCGGCTTCCAGc                   <  2:921397/80‑1 (MQ=255)                                                   ggCGGTGAGGACAATACAGACTTCCATCACTTTGACATCTACCGCCGTGCCAGTGGCAAAACCGGCGGCTTCCAGCCACt               >  1:921450/1‑80 (MQ=255)                                                    gCGGTGAGGACAATACAGACTTCCATCACTTTGACATCTACCGCCGTGCCAGTGGCAAAACCGGCGGCTTCCAGCCACTGa             >  2:921439/1‑81 (MQ=255)                                                      ggTGAGGACAATACAGACTTCCATCACTTTGACATCTACCGCCGTGCCAGTGGCAAAACCGGCGGCTTCCAGCCACTGAcc           >  2:921398/1‑81 (MQ=255)                                                       gTGAGGACAATACAGACTTCCATCACTTTGACATCTACCGCCGTGCCAGTGGCAAAACCGGCGGCTTCCAGCCACTGACCt          >  1:921446/1‑81 (MQ=255)                                                       gTGAGGACAATACAGACTTCCATCACTTTGACATCTACCGCCGTGCCAGTGGCAAAACCGGCGGCTTCCAGCCACTGACCt          >  2:921447/1‑81 (MQ=255)                                                          aGGACAATACAGACTTCCATCACTTTGACATCTACCGCCGTGCCAGTGGCAAAACCGGCGGCTTCCAGCCACTGACCttt        <  2:921399/80‑1 (MQ=255)                                                               aaTACAGACTTCCATCACTTTGACATCTACCGCCGTGCCAGTGGCAAAACCGGCGGCTTCCAGCCACTGACCTTTCAGGGt  >  1:921452/1‑81 (MQ=255)                                                                      |                                                                          ACCTGGCGTAGCGACTGCATCAGTTCGCTCTCCTCGGCGGCGGGTGGTTGGGCGGTGAGGACAATACAGCCTTCCATCACTTTGACATCTACCGCCGTGCCAGTGGCAAAACCGGCGGCTTCCAGCCACTGACCTTTCAGGGT  >  NC_000913/4579578‑4579720

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑