Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,590,284	G→A	F332F (TTC→TTT)	btsT ←	pyruvate:H(+) symporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,590,284	0	G	A	100.0%	161.5 / NA	44	F332F (TTC→TTT)	btsT	pyruvate:H(+) symporter
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (17/27);  total (17/27)

ATTAGCCAGCAGTTTTGGCGTCGTACCGGAAGAGATCAGCGCGTGGAAGCCAGATACCGCACCACAGGCGATGGTGATGAACAGGAACGGGAACAGAGCGCCTTTCCACAGCGGGCCAGTACCGTCAATGTACTGGGTCATGGCAGGCATTTTC  >  NC_000913/4590206‑4590359                                                                               |                                                                            aTTAGCCAGCAGTTTTGGCGTCGTACCGGAAGAGATCAGCGCGTGGAAGCCAGATACCGCACCACAGGCGATGGTGATaaa                                                                           <  1:923884/81‑1 (MQ=255)   tAGCCAGCAGTTTTGGCGTCGTACCGGAAGAGATCAGCGCGTGGAAGCCAGATACCGCACCACAGGCGATGGTGATaaa                                                                           >  2:923945/1‑79 (MQ=255)     gCCAGCAGTTTTGGCGTCGTACCGGAAGAGATCAGCGCGTGGAAGCCAGATACCGCACCACAGGCGATGGTGATAAACAg                                                                        >  1:923959/1‑80 (MQ=255)      ccAGCAGTTTTGGCGTCGTACCGGAAGAGATCAGCGCGTGGAAGCCAGATACCGCACCACAGGCGATGGTGATAAACAGGa                                                                      <  1:923885/81‑1 (MQ=255)       cagcagTTTTGGCGTCGTACCGGAAGAGATCAGCGCGTGGAAGCCAGATACCGCACCACAGGCGATGGTGATAAACAGGaa                                                                     <  2:923886/81‑1 (MQ=255)        agcagTTTTGGCGTCGTACCGGAAGAGATCAGCGCGTGGAAGCCAGATACCGCACCACAGGCGATGGTGATAAACAGGAAc                                                                    >  1:923955/1‑81 (MQ=255)          cagTTTTGGCGTCGTACCGGAAGAGATCAGCGCGTGGAAGCCAGATACCGCACCACAGGCGATGGTGATAAACAGGAACgg                                                                  <  2:923887/81‑1 (MQ=255)           agTTTTGGCGTCGTACCGGAAGAGATCAGCGCGTGGAAGCCAGATACCGCACCACAGGCGATGGTGATAAACAGGAACgg                                                                  >  1:923949/1‑80 (MQ=255)            gTTTTGGCGTCGTACCGGAAGAGATCAGCGCGTGGAAGCCAGATACCGCACCACAGGCGATGGTGATAAACAGGAACGGGa                                                                <  2:923888/81‑1 (MQ=255)                   cgtcgtACCGGAAGAGATCAGCGCGTGGAAGCCAGATACCGCACCACAGGCGATGGTGATAAACAGGAACGGGAACAGAgc                                                         <  1:923889/81‑1 (MQ=255)                       gtACCGGAAGAGATCAGCGCGTGGAAGCCAGATACCGCACCACAGGCGATGGTGATAAACAGGAACGGGAACAGAGCGcc                                                      <  1:923890/80‑1 (MQ=255)                       gtACCGGAAGAGATCAGCGCGTGGAAGCCAGATACCGCACCACAGGCGATGGTGATAAACAGGAACGGGAACAGAGCGCCt                                                     >  1:923891/1‑81 (MQ=255)                         aCCGGAAGAGATCAGCGCGTGGAAGCCAGATACCGCACCACAGGCGATGGTGATAAACAGGAACGGGAACAGAGCGCCttt                                                   >  1:923973/1‑81 (MQ=255)                             gAAGAGATCAGCGCGTGGAAGCCAGATACCGCACCACAGGCGATGGTGATAAACAGGAACGGGAACAGAGCGCCTTTCcac                                               <  1:923892/81‑1 (MQ=255)                              aaGAGATCAGCGCGTGGAAGCCAGATACCGCACCACAGGCGATGGTGATAAACAGGAACGGGAACAGAGCGCCTTTCcaca                                              <  2:923894/81‑1 (MQ=255)                              aaGAGATCAGCGCGTGGAAGCCAGATACCGCACCACAGGCGATGGTGATAAACAGGAACGGGAACAGAGCGCCTTTCcaca                                              <  1:923893/81‑1 (MQ=255)                                gagaTCAGCGCGTGGAAGCCAGATACCGCACCACAGGCGATGGTGATAAACAGGAACGGGAACAGAGCGCCTTTCCACAGc                                            <  1:923895/81‑1 (MQ=255)                                 agaTCAGCGCGTGGAAGCCAGATACCGCACCACAGGCGATGGTGATAAACAGGAACGGGAACAGAGCGCCTTTCCACAGCg                                           >  1:923896/1‑81 (MQ=255)                                  gaTCAGCGCGTGGAAGCCAGATACCGCACCACAGGCGATGGTGATAAACAGGAACGGGAACAGAGCGCCTTTCCACAGCgg                                          >  2:923976/1‑81 (MQ=255)                                      aGCGCGTGGAAGCCAGATACCGCACCACAGGCGATGGTGATAAACAGGAACGGGAACAGAGCGCCTTTCCACAGCGGGCCa                                      <  1:923897/81‑1 (MQ=255)                                        cgcgTGGAAGCCAGATACCGCACCACAGGCGATGGTGATAAACAGGAACGGGAACAGAGCGCCTTTCCACAGCGGGCCAGt                                    <  2:923898/81‑1 (MQ=255)                                           gTGGAAGCCAGATACCGCACCACAGGCGATGGTTATAAACAGGAACGGGAACAGAGCGCCTTTCCACAGCGGGCCAGTAcc                                 >  1:923970/1‑81 (MQ=255)                                            tGGAAGCCAGATACCGCACCACAGGCGATGGTGATAAACAGGAACGGGAACAGAGCGCCTTTCCACAGCGGGCCAGTACCg                                >  2:923962/1‑81 (MQ=255)                                              gAAGCCAGATACCGCACCACAGGCGATGGTGATAAACAGGAACGGGAACAGAGCGCCTTTCCACAGCGGGCCAGTACCGTc                              <  1:923899/81‑1 (MQ=255)                                                 gCCAGATACCGCACCACAGGCGATGGTGATAAACAGGAACGGGAACAGAGCGCCTTTCCACAGCGGGCCAGTACCGTCAAt                           >  2:923974/1‑81 (MQ=255)                                                 gCCAGATACCGCACCACAGGCGATGGTGATAAACAGGAACGGGAACAGAGCGCCTTTCCACAGCGGGCCAGTACCGTCAAt                           <  1:923900/81‑1 (MQ=255)                                                   cAGATACCGCACCACAGGCGATGGTGATAAACAGGAACGGGAACAGAGCGCCTTTCCACAGCGGGCCAGTACCGTCAATg                          >  1:923969/1‑80 (MQ=255)                                                   cAGATACCGCACCACAGGCGATGGTGATAAACAGGAACGGGAACAGAGCGCCTTTCCACAGCGGGCCAGTACCGTCAATGt                         <  2:923901/81‑1 (MQ=255)                                                      aTACCGCACCACAGGCGATGGTGATAAACAGGAACGGGAACAGAGCGCCTTTCCACAGCGGGCCAGTACCGTCAATGTACt                      <  1:923902/81‑1 (MQ=255)                                                        aCCGCACCACAGGCGATGGTGATAAACAGGAACGGGAACAGAGCGCCTTTCCACAGCGGGCCAGTACCGTCAATGTACTgg                    >  2:923977/1‑81 (MQ=255)                                                         ccGCACCACAGGCGATGGTGATAAACAGGAACGGGAACAGAGCGCCTTTCCACAGCGGGCCAGTACCGTCAATGTACTgg                    <  1:923903/80‑1 (MQ=255)                                                          cGCACCACAGGCGATGGTGATAAACAGGAACGGGAACAGAGCGCCTTTCCACAGCGGGCCAGTACCGTCAATGTACTGGGt                  <  1:923906/81‑1 (MQ=255)                                                          cGCACCACAGGCGATGGTGATAAACAGGAACGGGAACAGAGCGCCTTTCCACAGCGGGCCAGTACCGTCAATGTACTGGGt                  <  1:923905/81‑1 (MQ=255)                                                          cGCACCACAGGCGATGGTGATAAACAGGAACGGGAACAGAGCGCCTTTCCACAGCGGGCCAGTACCGTCAATGTACTGGGt                  <  2:923904/81‑1 (MQ=255)                                                           gCACCACAGGCGATGGTGATAAACAGGAACGGGAACAGAGCGCCTTTCCACAGCGGGCCAGTACCGTCAATGTACTGGGt                  >  1:923965/1‑80 (MQ=255)                                                           gCACCACAGGCGATGGTGATAAACAGGAACGGGAACAGAGCGCCTTTCCACAGCGGGCCAGTACCGTCAATGTACTGGGTc                 <  1:923907/81‑1 (MQ=255)                                                             accacAGGCGATGGTGATAAACAGGAACGGGAACAGAGCGCCTTTCCACAGCGGGCCAGTACCGTCAATGTACTGGGTCAt               >  2:923982/1‑81 (MQ=255)                                                               cacaGGCGATGGTGATAAACAGGAACGGGAACAGAGCGCCTTTCCACAGCGGGCCAGTACCGTCAATGTACTGGGTCATgg             <  1:923908/81‑1 (MQ=255)                                                                acaGGCGATGGTGATAAACAGGAACGGGAACAGAGCGCCTTTCCACAGCGGGCCAGTACCGTCAATGTACTGGGTCATggc            <  2:923909/81‑1 (MQ=255)                                                                   ggCGATGGTGATAAACAGGAACGGGAACAGAGCGCCTGTCCACAGCGGGCCAGTACCGTCAATGTACTGGGTTATggcagg         <  2:923910/81‑1 (MQ=37)                                                                    gCGATGGTGATAAACAGGAACGGGAACAGAGCGCCTTTCCACAGCGGGCCAGTACCGTCAATGTACTGGGTCATggcagg         >  2:923972/1‑80 (MQ=255)                                                                    gCGATGGTGATAAACAGGAACGGGAACAGAGCGCCTTTCCACAGCGGGCCAGTACCGTCAATGTACTGGGTCATggcagg         >  2:923980/1‑80 (MQ=255)                                                                      gATGGTGATAAACAGGAACGGGAACAGAGCGCCTTTCCACAGCGGGCCAGTACCGTCAATGTACTGGGTCATGGCAGGCAt      >  2:923975/1‑81 (MQ=255)                                                                        tGGTGATAAACAGGAACGGGAACAGAGCGCCTTTCCACAGCGGGCCAGTACCGTCAATGTACTGGGTCATGGCAGGCAttt    <  1:923911/81‑1 (MQ=255)                                                                        tGGTGATAAACAGGAACGGGAACAGAGCGCCTTTCCACAGAGGGCCAGTACCGTCAATGTACTGGGTCATGGCAGGCAttt    <  2:923912/81‑1 (MQ=255)                                                                          gTGATAAACAGGAACGGGAACAGAGCGCCTTTCCACAGCGGGCCAGTACCGTCAATGTACTGGGTCATGGCAGGCATTTTc  <  1:923913/81‑1 (MQ=255)                                                                               |                                                                            ATTAGCCAGCAGTTTTGGCGTCGTACCGGAAGAGATCAGCGCGTGGAAGCCAGATACCGCACCACAGGCGATGGTGATGAACAGGAACGGGAACAGAGCGCCTTTCCACAGCGGGCCAGTACCGTCAATGTACTGGGTCATGGCAGGCATTTTC  >  NC_000913/4590206‑4590359

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑