Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,635,821	G→A	G101S (GGC→AGC)	creA →	PF05981 family protein CreA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,635,821	0	G	A	100.0%	103.8 / NA	29	G101S (GGC→AGC)	creA	PF05981 family protein CreA
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (20/9);  total (20/9)

ATGCGGCCATTTCTTGTCAGCAAGTCGGGCCGATTGAACTGTCGGATCGTATTAAAAACGGCAAAGCTCAGGGCGAGGTAGTATTCAAAAAACGCACGTCCCTGGTCTTTAAGTCGTTACAGGTCGTGCGCTTTTATGATGCCAAAC  >  NC_000913/4635750‑4635896                                                                        |                                                                            aTGCGGCCATTTCTTGTCAGCAAGTCGGGCCGATTGAACTGTCGGATCGTATTAAAAACGGCAAAGCTCAGAGCGAGgtag                                                                    >  1:934479/1‑81 (MQ=255) aTGCGGCCATTTCTTGTCAGCAAGTCGGGCCGATTGAACTGTCGGATCGTATTAAAAACGGCAAAGCTCAGAGCGAGgtag                                                                    >  1:934495/1‑81 (MQ=255)  tGCGGCCATTTCTTGTCAGCAAGTCGGGCCGATTGAACTGTCGGATCGTATTAAAAACGGCAAAGCTCAGAGCGGGgtagt                                                                   <  2:934448/81‑1 (MQ=255)    cGGCCATTTCTTGTCAGCAAGTCGGGCCGATTGAACTGTCGGATCGTATTAAAAACGGCAAAGCTCAGAGCGAGGTAGTAt                                                                 <  1:934449/81‑1 (MQ=255)     ggCCATTTCTTGTCAGCAAGTCGGGCCGATTGAACTGTCGGATCGTATTAAAAACGGCAAAGCTCAGAGCGAGGTAGTAtt                                                                >  2:934483/1‑81 (MQ=255)        cATTTCTTGTCAGCAAGTCGGGCCGATTGAACTGTCGGATCGTATTAAAAACGGCAAAGCTCAGAGCGAGGTAGTATTCa                                                              >  2:934488/1‑80 (MQ=255)                gTCAGCAAGTCGGGCCGATTGAACTGTCGGATCGTATTAAAAACGGCAAAGCTCAGAGCGAGGTAGTATTCAAAAAACGCa                                                     >  2:934502/1‑81 (MQ=255)                 tCAGCAAGTCGGGCCGATTGAACTGTCGGATCGTATTAAAAACGGCAAAGCTCAGAGCGAGGTAGTATTCAAAAAACGCAc                                                    <  2:934450/81‑1 (MQ=255)                      aaGTCGGGCCGATTGAACTGTCGGATCGTATTAAAAACGGCAAAGCTCAGAGCGAGGTAGTATTCAAAAAACGCACGTccc                                               >  1:934493/1‑81 (MQ=255)                       aGTCGGGCCGATTGAACTGTCGGATCGTATTAAAAACGGCAAAGCTCAGAGCGAGGTAGTATTCAAAAAACGCACGTCCCt                                              >  1:934472/1‑81 (MQ=255)                       aGTCGGGCCGATTGAACTGTCGGATCGTATTAAAAACGGCAAAGCTCAGAGCGAGGTAGTATTCAAAAAACGCACGTCCCt                                              >  2:934480/1‑81 (MQ=255)                          cGGGCCGATTGAACTGTCGGATCGTATTAAAAACGGCAAAGCTCAGAGCGAGGTAGTATTCAAAAAACGCACGTCCCTGGt                                           >  2:934478/1‑81 (MQ=255)                            ggCCGATTGAACTGTCGGATCGTATTAAAAACGGCAAAGCTCAGAGCGAGGTAGTATTCAAAAAACGCACGTCCCTGGTCt                                         <  2:934451/81‑1 (MQ=255)                             gCCGATTGAACTGTTGGATCGTATTAAAAACGGCAAAGCTCAGAGCGAGGTAGTATTCAAAAAACGCACGTCCCTGGTCtt                                        >  1:934489/1‑81 (MQ=255)                              ccGATTGAACTGTCGGATCGTATTAAAAACGGCAAAGCTCAGAGCGAGGTAGTATTCAAAAAACGCACGTCCCTGGTCttt                                       <  2:934452/81‑1 (MQ=255)                                    gAACTGTCGGATCGTATTAAAAACGGCAAAGCTCAGAGCGAGGTAGTATTCAAAAAACGCACGTCCCTGGTCTTTAAGTCg                                 >  1:934509/1‑81 (MQ=255)                                            ggATCGTATTAAAAACGGCAAAGCTCAGAGCGAGGTAGTATTCAAAAAACGCACGTCCCTGGTCTTTAAGTCGTTACAGGt                         <  1:934453/81‑1 (MQ=255)                                             gATCGTATTAAAAACGGCAAAGCTCAGAGCGAGGTAGTATTCAAAAAACGCACGTCCCTGGTCTTTAAGTCGTTACAGGTc                        <  1:934454/81‑1 (MQ=255)                                              aTCGTATTAAAAACGGCAAAGCTCAGAGCGAGGTAGTATTCAAAAAACGCACGTCCCTGGTCTTTAAGTCGTTACAGGTCg                       >  1:934494/1‑81 (MQ=255)                                                 gTATTAAAAACGGCAAAGCTCAGAGCGAGGTAGTATTCAAAAAACGCACGTCCCTGGTCTTTAAGTCGTTACAGGTCGTgc                    >  1:934503/1‑81 (MQ=255)                                                 gTATTAAAAACGGCAAAGCTCAGAGCGAGGTAGTATTCAAAAAACGCACGTCCCTGGTCTTTAAGTCGTTACAGGTCGTgc                    >  1:934481/1‑81 (MQ=255)                                                 gTATTAAAAACGGCAAAGCTCAGAGCGAGGTAGTATTCAAAAAACGCACGTCCCTGGTCTTTAAGTCGTTACAGGTCGTgc                    <  1:934455/81‑1 (MQ=255)                                                     tAAAAACGGCAAAGCTCAGAGCGAGGTAGTATTCAAAAAACGCACGTCCCTGGTCTTTAAGTCGTTACAGGTCGTGCGCtt                >  2:934505/1‑81 (MQ=255)                                                        aaaCGGCAAAGCTCAGAGCGAGGTAGTATTCAAAAAACGCACGTCCCTGGTCTTTAAGTCGTTACAGGTCGTGCGCTTTTa             <  2:934456/81‑1 (MQ=255)                                                        aaaCGGCAAAGCTCAGAGCGAGGTAGTATTCAAAAAACGCACGTCCCTGGTCTTTAAGTCGTTACAGGTCGTGCGCTTTTa             >  1:934500/1‑81 (MQ=255)                                                         aaCGGCAAAGCTCAGAGCGAGGTAGTATTCAAAAAACGCACGTCCCTGGTCTTTAAGTCGTTACAGGTCGTGCGCTTTTat            >  1:934491/1‑81 (MQ=255)                                                                 aGCTCAGAGCGAGGTAGTATTCAAAAAACGCACGTCCCTGGTCTTTAAGTCGTTACAGGTCGTGCGCTTTTATGATGCCa     >  1:934498/1‑80 (MQ=255)                                                                   cTCAGAGCGAGGTAGTATTCAAAAAACGCACGTCCCTGGTCTTTAAGTCGTTACAGGTCGTGCGCTTTTATGATGCCAaac  >  1:934511/1‑81 (MQ=255)                                                                   cTCAGAGCGAGGTAGTATTCAAAAAACGCACGTCCCTGGTCTTTAAGTCGTTACAGGTCGTGCGCTTTTATGATGCCAaac  >  2:934497/1‑81 (MQ=255)                                                                        |                                                                            ATGCGGCCATTTCTTGTCAGCAAGTCGGGCCGATTGAACTGTCGGATCGTATTAAAAACGGCAAAGCTCAGGGCGAGGTAGTATTCAAAAAACGCACGTCCCTGGTCTTTAAGTCGTTACAGGTCGTGCGCTTTTATGATGCCAAAC  >  NC_000913/4635750‑4635896

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑