Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	458,055	A→T	A210A (GCA→GCT)	clpX →	ATP‑dependent Clp protease ATP‑binding subunit ClpX

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	458,055	0	A	T	100.0%	135.1 / NA	38	A210A (GCA→GCT)	clpX	ATP‑dependent Clp protease ATP‑binding subunit ClpX
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (18/20);  total (18/20)

TGAAATCGACAAGATTTCTCGTAAGTCAGACAACCCGTCCATTACCCGAGACGTTTCCGGTGAAGGCGTACAGCAGGCACTGTTGAAACTGATCGAAGGTACGGTAGCTGCTGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTT  >  NC_000913/457977‑458132                                                                               |                                                                              tGAAATCGACAAGATTTCTCGTAAGTCAGACAACCGGTCCATTACCCGAGACGTTTCCGGTGAAGGCGTACAGCAGGctct                                                                             <  2:97363/81‑1 (MQ=255)   aaaTCGACAAGATTTCTCGTAAGTCAGACAACCCGTCCATTACCCGAGACGTTTCCGGTGAAGGCGTACAGCAGGCTCTg                                                                            <  1:97364/80‑1 (MQ=255)   aaaTCGACAAGATTTCTCGTAAGTCAGACAACCCGTCCATTACCCGAGACGTTTCCGGTGAAGGCGTACAGCAGGCTCTGt                                                                           >  2:97419/1‑81 (MQ=255)    aaTCGACAAGATTTCTCGTAAGTCAGACAACCCGTCCATTACCCGAGACGTTTCCGGTGAAGGCGTACAGCAGGCTCTGtt                                                                          <  2:97365/81‑1 (MQ=255)     aTCGACAAGATTTCTCGTAAGTCAGACAACCCGTCCATTACCCGAGACGTTTCCGGTGAAGGCGTACAGCAGGCTCTGTTg                                                                         <  1:97366/81‑1 (MQ=255)        gACAAGATTTCTCGTAAGTCAGACAACCCGTCCATTACCCGAGACGTTTCCGGTGAAGGCGTACAGCAGGCTCTGTTGaaa                                                                      <  2:97367/81‑1 (MQ=255)         aCAAGATTTCTCGTAAGTCAGACAACCCGTCCATTACCCGAGACGTTTCCGGTGAAGGCGTACAGCAGGCTCTGTTGAAAc                                                                     >  1:97423/1‑81 (MQ=255)           aaGATTTCTCGTAAGTCAGACAACCCGTCCATTACCCGAGACGTTTCCGGTGAAGGCGTACAGCAGGCTCTGTTGAAACt                                                                    >  2:97414/1‑80 (MQ=255)                      tAAGTCAGACAACCCGTCCATTACCCGAGACGTTTCCGGTGAAGGCGTACAGCAGGCTCTGTTGAAACTGATCGAAGGTAc                                                        >  1:97415/1‑81 (MQ=255)                         gTCAGACAACCCGTCCATTACCCGAGACGTTTCCGGTGAAGGCGTACAGCAGGCTCTGTTGAAACTGATCGAAGGTACGGt                                                     <  1:97369/81‑1 (MQ=255)                         gTCAGACAACCCGTCCATTACCCGAGACGTTTCCGGTGAAGGCGTACAGCAGGCTCTGTTGAAACTGATCGAAGGTACGGt                                                     <  1:97368/81‑1 (MQ=255)                          tCAGACAACCCGTCCATTACCCGAGACGTTTCCGGTGAAGGCGTACAGCAGGCTCTGTTGAAACTGATCGAAGGTACGGTa                                                    <  2:97370/81‑1 (MQ=255)                                aaCCCGTCCATTACCCGAGACGTTTCCGGTGAAGGCGTACAGCAGGCTCTGTTGAAACTGATCGAAGGTACGGTAgctgct                                              <  1:97372/81‑1 (MQ=255)                                aaCCCGTCCATTACCCGAGACGTTTCCGGTGAAGGCGTACAGCAGGCTCTGTTGAAACTGATCGAAGGTACGGTAgctgct                                              <  2:97371/81‑1 (MQ=255)                                aaCCCGTCCATTACCCGAGACGTTTCCGGTGAAGGCGTACAGCAGGCTCTGTTGAAACTGATCGAAGGTACGGTAgctgct                                              >  1:97412/1‑81 (MQ=255)                                aaCCCGTCCATTACCCGAGACGTTTCCGGTGAAGGCGTACAGCAGGCTCTGTTGAAACTGATCGAAGGTACGGTAgctgct                                              >  2:97426/1‑81 (MQ=255)                                  cccGTCCATTACCCGAGACGTTTCCGGTGAAGGCGTACAGCAGGCTCTGTTGAAACTGATCGAAGGTACGGTAGCTGCTGt                                            <  1:97373/81‑1 (MQ=255)                                   ccGTCCATTACCCGAGACGTTTCCGGTGAAGGCGTACAGCAGGCTCTGTTGAAACTGATCGAAGGTACGGTAGCTGCTGtt                                           >  2:97422/1‑81 (MQ=255)                                       ccATTACCCGAGACGTTTCCGGTGAAGGCGTACAGCAGGCTCTGTTGAAACTGATCGAAGGTACGGTAGCTGCTGTTCCAc                                       >  2:97431/1‑81 (MQ=255)                                          ttACCCGAGACGTTTCCGGTGAAGGCGTACAGCAGGCTCTGTTGAAACTGATCGAAGGTACGGTAGCTGCTGTTCCACCg                                     >  1:97425/1‑80 (MQ=255)                                            aCCCGAGACGTTTCCGGTGAAGGCGTACAGCAGGCTCTGTTGAAACTGATCGAAGGTACGGTAGCTGCTGTTCCACCGc                                    >  1:97432/1‑79 (MQ=255)                                            aCCCGAGACGTTTCCGGTGAAGGCGTACAGCAGGCTCTGTTGAAACTGATCGAAGGTACGGTAGCTGCTGTTCCACCGCa                                   <  1:97374/80‑1 (MQ=255)                                                  gaCGTTTCCGGTGAAGGCGTACAGCAGGCTCTGTTGAAACTGATCGAAGGTACGGTAGCTGCTGTTCCACCGCAAGGTggg                            <  1:97375/81‑1 (MQ=255)                                                   aCGTTTCCGGTGAAGGCGTACAGCAGGCTCTGTTGAAACTGATCGAAGGTACGGTAGCTGCTGTTCCACCGCAAGGTGGGc                           <  1:97376/81‑1 (MQ=255)                                                     gTTTCCGGTGAAGGCGTACAGCAGGCTCTGTTGAAACTGATCGAAGGTACGGTAGCTGCTGTTCCACCGCAAGGTGGGCGt                         <  1:97377/81‑1 (MQ=255)                                                        tCCGGTGAAGGCGTACAGCAGGCTCTGTTGAAACTGATCGAAGGTACGGTAGCTGCTGTTCCACCGCAAGGTGGGCGTaaa                      >  2:97417/1‑81 (MQ=255)                                                         ccGGTGAAGGCGTACAGCAGGCTCTGTTGAAACTGATCGAAGGTACGGTAGCTGCTGTTCCACCGCAAGGTGGGCGTaaa                      >  2:97428/1‑80 (MQ=255)                                                           ggTGAAGGCGTACAGCAGGCTCTGTTGAAACTGATCGAAGGTACGGTAGCTGCTGTTCCACCGCAAGGTGGGCGTAAACAt                   >  1:97420/1‑81 (MQ=255)                                                           ggTGAAGGCGTACAGCAGGCTCTGTTGAAACTGATCGAAGGTACGGTAGCTGCTGTTCCACCGCAAGGTGGGCGTAAACAt                   <  2:97378/81‑1 (MQ=255)                                                             tGAAGGCGTACAGCAGGCTCTGTTGAAACTGATCGAAGGTACGGTAGCTGCTGTTCCACCGCAAGGTGGGCGTAAACATcc                 <  1:97379/81‑1 (MQ=255)                                                               aaGGCGTACAGCAGGCTCTGTTGAAACTGATCGAAGGTACGGTAGCTGCTGTTCCACCGCAAGGTGGGCGTAAACATCCg                >  1:97416/1‑80 (MQ=255)                                                                  gCGTACAGCAGGCTCTGTTGAAACTGATCGAAGGTACGGTAGCTGCTGTTCCACCGCAAGGTGGGCGTAAACATCCgcag             >  1:97418/1‑80 (MQ=255)                                                                   cGTACAGCAGGCTCTGTTGAAACTGATCGAAGGTACGGTAGCTGCTGTTCCACCGCAAGGTGGGCGTAAACATCCgcagca           <  1:97381/81‑1 (MQ=255)                                                                    gTACAGCAGGCTCTGTTGAAACTGATCGAAGGTACGGTAGCTGCTGTTCCACCGCAAGGTGGGCGTAAACATCCGcagcag          <  1:97382/81‑1 (MQ=255)                                                                       cagcagGCTCTGTTGAAACTGATCGAAGGTACGGTAGCTGCTGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGaa       >  2:97438/1‑81 (MQ=255)                                                                           agGCTCTGTTGAAACTGATCGAAGGTACGGTAGCTGCTGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCt   >  2:97424/1‑81 (MQ=255)                                                                            ggCTCTGTTGAAACTGATCGAAGGTACGGTAGCTGCTGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCtt  <  2:97384/81‑1 (MQ=255)                                                                            ggCTCTGTTGAAACTGATCGAAGGTACGGTAGCTGCTGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCtt  <  1:97383/81‑1 (MQ=255)                                                                            ggCTCTGTTGAAACTGATCGAAGGTACGGTAGCTGCTGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCtt  >  2:97436/1‑81 (MQ=255)                                                                               |                                                                              TGAAATCGACAAGATTTCTCGTAAGTCAGACAACCCGTCCATTACCCGAGACGTTTCCGGTGAAGGCGTACAGCAGGCACTGTTGAAACTGATCGAAGGTACGGTAGCTGCTGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTT  >  NC_000913/457977‑458132

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑