Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	475,050	G→A	Q38* (CAG→TAG)	tesB ←	acyl‑CoA thioesterase II

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	475,050	0	G	A	100.0%	83.0 / NA	24	Q38* (CAG→TAG)	tesB	acyl‑CoA thioesterase II
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (8/16);  total (8/16)

GTAGCTGTGAAACGAATGTACCAGCCGCTCTTCAGGGACGGTCTCTTTTGCAGCATACAAGGCCTGACCCACGACCTGGCCGCCAAACACCTGGCGTAAACCTAAATCTTCACTCTGGCCGCGAAAGAGTCCTTCCTCAATTTTTTCCAGATT  >  NC_000913/474973‑475125                                                                              |                                                                            gTAGCTGTGAAACGAATGTACCAGCCGCTCTTCAGGGACGGTCTCTTTTGCAGCATACAAGGCCTGACCCACGACCTAgcc                                                                          >  1:100848/1‑81 (MQ=255) gTAGCTGTGAAACGAATGTACCAGCCGCTCTTCAGGGACGGTCTCTTTTGCAGCATACAAGGCCTGACCCACGACCTAgcc                                                                          >  2:100852/1‑81 (MQ=255)        tgAAACGAATGTACCAGCCGCTCTTCAGGGACGGTCTCTTTTGCAGCATACAAGGCCTGACCCACGACCTAGCCGCCaa                                                                     <  1:100812/79‑1 (MQ=255)          aaaCGAATGTACCAGCCGCTCTTCAGGGACGGTCTCTTTTGCAGCATACAAGGCCTGACCCACGACCTAGCCGCCAAaca                                                                  <  2:100813/80‑1 (MQ=255)               aaTGTACCAGCCGCTCTTCAGGGACGGTCTCTTTTGCAGCATACAAGGCCTGACCCACGACCTAGCCGCCAAACACCTGGc                                                            <  1:100814/81‑1 (MQ=255)                    aCCAGCCGCTCTTCAGGGACGGTCTCTTTTGCAGCATACAAGGCCTGACCCACGACCTAGCCGCCAAACACCTGGCGTaaa                                                       >  2:100853/1‑81 (MQ=255)                       aGCCGCTCTTCAGGGACGGTCTCTTTTGCAGCATACAAGGCCTGACCCACGACCTAGCCGCCAAACACCTGGCGTAAACCt                                                    <  2:100815/81‑1 (MQ=255)                        gCCGCTCTTCAGGGACGGTCTCTTTTGCAGCATACAAGGCCTGACCCACGACCTAGCCGCCAAACACCTGGCGTAAACCTa                                                   <  1:100816/81‑1 (MQ=255)                            ctctTCAGGGACGGTCTCTTTTGCAGCATACAAGGCCTGACCCACGACCTAGCCGCCAAACACCTGGCGTAAACCTAAATc                                               <  1:100817/81‑1 (MQ=255)                             tctTCAGGGACGGTCTCTTTTGCAGCATACAAGGCCTGACCCACGACCTAGCCGCCAAACACCTGGCGTAAACCTAAATCt                                              >  2:100859/1‑81 (MQ=255)                                 cAGGGACGGTCTCTTTTGCAGCATACAAGGCCTGACCCACGACCTAGCCGCCAAACACCTGGCGTAAACCTAAATCtt                                             <  2:100818/78‑1 (MQ=255)                                 cAGGGACGGTCTCTTTTGCAGCATACAAGGCCTGACCCACGACCTAGCCGCCAAACACCTGGCGTAAACCTAAATCtt                                             <  2:100819/78‑1 (MQ=255)                                 cAGGGACGGTCTCTTTTGCAGCATACAAGGCCTGACCCACGACCTAGCCGCCAAACACCTGGCGTAAACCTAAATCTTCAc                                          >  1:100851/1‑81 (MQ=255)                                      aCGGTCTCTTTTGCAGCATACAAGGCCTGACCCACGACCTAGCCGCCAAACACCTGGCGTAAACCTAAATCTTCACTCTgg                                     <  2:100820/81‑1 (MQ=255)                                        ggTCTCTTTTGCAGCATACAAGGCCTGACCCACGACCTAGCCGCCAAACACCTGGCGTAAACCTAAATCTTCACTCTGGcc                                   <  2:100821/81‑1 (MQ=255)                                        ggTCTCTTTTGCAGCATACAAGGCCTGACCCACGACCTAGCCGCCAAACACCTGGCGTAAACCTAAATCTTCACTCTGGcc                                   >  1:100860/1‑81 (MQ=255)                                         gTCTCTTTTGCAGCATACAAGGCCTGACCCACGACCTAGCCGCCAAACACCTGGCGTAAACCTAAATCTTCACTCTGGCcg                                  <  2:100822/81‑1 (MQ=255)                                                      caTACAAGGCCTGACCCACGACCTAGCCGCCAAACACCTGGCGTAAACCTAAATCTTCACTCTGGCCGCGAAAGAGtcctt                     <  1:100823/81‑1 (MQ=255)                                                       aTACAAGGCCTGACCCACGACCTAGCCGCCAAACACCTGGCGTAAACCTAAATCTTCACTCTGGCCGCGAAAGAGtccttc                    <  2:100824/81‑1 (MQ=255)                                                         aCAAGGCCTGACCCACGACCTAGCCGCCAAACACCTGGCGTAAACCTAAATCTTCACTCTGGCCGCGAAAGAGtccttcct                  >  2:100849/1‑81 (MQ=255)                                                          cAAGGCCTGACCCACGACCTAGCCGCCAAACACCTGGCGTAAACCTAAATCTTCACTCTGGCCGCGAAAGAGTCCTTCCTc                 <  2:100825/81‑1 (MQ=255)                                                           aaGGCCTGACCCACGACCTAGCCGCCAAACACCTGGCGTAAACCTAAATCTTCACTCTGGCCGCGAAAGAGTCCTTCCTCa                <  2:100826/81‑1 (MQ=255)                                                            aGGCCTGACCCACGACCTAGCCGCCAAACACCTGGCGTAAACCTAAATCTTCACTCTGGCCGCGAAAGAGTCCTTCCTCaa               >  2:100856/1‑81 (MQ=255)                                                                         gACCTAGCCGCCAAACACCTGGCGTAAACCTAAATCTTCACTCTGGCCGCGAAAGAGTCCTTCCTAAATTTTTTCCAGAtt  <  2:100827/81‑1 (MQ=255)                                                                              |                                                                            GTAGCTGTGAAACGAATGTACCAGCCGCTCTTCAGGGACGGTCTCTTTTGCAGCATACAAGGCCTGACCCACGACCTGGCCGCCAAACACCTGGCGTAAACCTAAATCTTCACTCTGGCCGCGAAAGAGTCCTTCCTCAATTTTTTCCAGATT  >  NC_000913/474973‑475125

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑