Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	475,469	C→T	P31S (CCG→TCG)	ybaY →	PF09619 family lipoprotein YbaY

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	475,469	0	C	T	100.0%	133.4 / NA	37	P31S (CCG→TCG)	ybaY	PF09619 family lipoprotein YbaY
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (21/16);  total (21/16)

CACATGGCCAGTGGTTTAGCGGTTGCGATTGCGTTGGCGGCTTGCGCAGATAAAAGCGCGGATATTCAGACGCCAGCACCGGCTGCAAATACGTCTATTTCAGCAACACAACAACCAGCTATCCAGCAACCGAATGTCTCCGGTACCGTCTG  >  NC_000913/475391‑475542                                                                               |                                                                          cacaTGGCCAGTGGTTTAGCGGTTGCGATTGCGTTGGCGGCTTGCGCAGATAAAAGCGCGGATATTCAGACGCCAGCATCg                                                                         <  1:100877/81‑1 (MQ=255)    aTGGCCAGTGGTTTAGCGGTTGCGATTGCGTTGGCGGCTTGCGCAGATAAAAGCGCGGATATTCAGACGCCAGCATCGGCt                                                                      >  2:100908/1‑81 (MQ=255)     tGGCCAGTGGTTTAGCGGTTGCGATTGCGTTGGCGGCTTGCGCAGATAAAAGCGCGGATATTCAGACGCCAGCATCGGCTg                                                                     >  1:100918/1‑81 (MQ=255)      ggCCAGTGGTTTAGCGGTTGCGATTGCGTTGGCGGCTTGCGCAGATAAAAGCGCGGATATTCAGACGCCAGCATCGGCTg                                                                     >  2:100920/1‑80 (MQ=255)       gCCAGTGGTTTAGCGGTTGCGATTGCGTTGGCGGCTTGCGCAGATAAAAGCGCGGATATTCAGACGTCAGCATCGGCTGCa                                                                   <  1:100878/81‑1 (MQ=255)          aGTGGTTTAGCGGTTGCGATTGCGATGGCGGCTTGCGCAGATAAAAGCGCGGATATTCAGACGCCAGCATCGGCTGCAAAt                                                                <  2:100879/81‑1 (MQ=255)           gTGGTTTAGCGGTTGCGATTGCGTTGGCGGCTTGCGCAGATAAAAGCGCGGATATTCAGACGCCAGCATCGGCTGCAAATa                                                               >  2:100910/1‑81 (MQ=255)                   gCGGTTGCGATTGCGTTGGCGGCTTGCGCAGATAAAAGCGCGGATATTCAGACGCCAGCATCGGCTGCAAATACGTCTAtt                                                       >  1:100905/1‑81 (MQ=255)                    cGGTTGCGATTGCGTTGGCGGCTTGCGCAGATAAAAGCGCGGATATTCAGACGCCAGCATCGGCTGCAAATACGTCTAttt                                                      >  1:100915/1‑81 (MQ=255)                     ggTTGCGATTGCGTTGGCGGCTTGCGCAGATAAAAGCGCGGATATTCAGACGCCAGCATCGGCTGCAAATACGTCTATTTc                                                     <  1:100880/81‑1 (MQ=255)                     ggTTGCGATTGCGTTGGCGGCTTGCGCAGATAAAAGCGCGGATATTCAGACGCCAGCATCGGCTGCAAATACGTCTATTTc                                                     <  2:100881/81‑1 (MQ=255)                       ttGCGATTGCGTTGGCGGCTTGCGCAGATAAAAGCGCGGATATTCAGACGCCAGCATCGGCTGCAAATACGTCTATTTCAg                                                   >  2:100914/1‑81 (MQ=255)                         gCGATTGCGTTGGCGGCTTGCGCAGATAAAAGCGCGGATATTCAGACGCCAGCATCGGCTGCAAATACGTCTATTTCAGc                                                  <  1:100883/80‑1 (MQ=255)                         gCGATTGCGTTGGCGGCTTGCGCAGATAAAAGCGCGGATATTCAGACGCCAGCATCGGCTGCAAATACGTCTATTTCAGc                                                  <  1:100882/80‑1 (MQ=255)                             ttGCGTTGGCGGCTTGCGCAGATAAAAGCGCGGATATTCAGACGCCAGCATCGGCTGCAAATACGTCTATTTCAGcaacac                                             <  1:100884/81‑1 (MQ=255)                              tGCGTTGGCGGCTTGCGCAGATAAAAGCGCGGATATTCAGACGCCAGCATCGGCTGCAAATACGTCTATTTCAGcaacac                                             >  2:100923/1‑80 (MQ=255)                                 gTTGGCGGCTTGCGCAGATAAAAGCGCGGATATTCAGACGCCAGCATCGGCTGCAAATACGTCTATTTCAGcaacacaac                                          >  1:100917/1‑80 (MQ=255)                                     gcggcTTGCGCAGATAAAAGCGCGGATATTCAGACGCCAGCATCGGCTGCAAATACGTCTATTTCAGCAACACAACAAcc                                      <  2:100886/80‑1 (MQ=255)                                     gcggcTTGCGCAGATAAAAGCGCGGATATTCAGACGCCAGCATCGGCTGCAAATACGTCTATTTCAGCAACACAACAAcc                                      <  1:695230/80‑1 (MQ=255)                                     gcggcTTGCGCAGATAAAAGCGCGGATATTCAGACGCCAGCATCGGCTGCAAATACGTCTATTTCAGCAACACAACAAcc                                      <  1:100885/80‑1 (MQ=255)                                      cggcTTGCGCAGATAAAAGCGCGGATATTCAGACGCCAGCATCGGCTGCAAATACGTCTATTTCAGCAACACAACAACCAg                                    <  1:100887/81‑1 (MQ=255)                                       ggcTTGCGCAGATAAAAGCGCGGATATTCAGACGCCAGCATCGGCTGCAAATACGTCTATTTCAGCAACACAACAACCAGc                                   >  2:100939/1‑81 (MQ=255)                                         cTTGCGCAGATAAAAGCGCGGATATTCAGACGCCAGCATCGGCTGCAAATACGTCTATTTCAGCAACACAACAACCAGCTa                                 >  1:100952/1‑81 (MQ=255)                                                    aaaaGCGCGGATATTCAGACGCCAGCATCGGCTGCAAATACGTCTATTTCAGCAACACAACAACCAGCTATCCAGCAAcc                       >  2:100924/1‑80 (MQ=255)                                                     aaaGCGCGGATATTCAGACGCCAGCATCGGCTGCAAATACGTCTATTTCAGCAACACAACAACCAGCTATCCAGCAACCg                      <  1:100889/80‑1 (MQ=255)                                                     aaaGCGCGGATATTCAGACGCCAGCATCGGCTGCAAATACGTCTATTTCAGCAACACAACAACCAGCTATCCAGCAACCGa                     <  2:100888/81‑1 (MQ=255)                                                      aaGCGCGGATATTCAGACGCCAGCATCGGCTGCAAATACGTCTATTTCAGCAACACAACAACCAGCTATCCAGCAACCGaa                    >  1:100950/1‑81 (MQ=255)                                                       aGCGCGGATATTCAGACGCCAGCATCGGCTGCAAATACGTCTATTTCAGCAACACAACAACCAGCTATCCAGCAACCGAAt                   <  2:100891/81‑1 (MQ=255)                                                       aGCGCGGATATTCAGACGCCAGCATCGGCTGCAAATACGTCTATTTCAGCAACACAACAACCAGCTATCCAGCAACCGAAt                   <  2:100890/81‑1 (MQ=255)                                                             gATATTCAGACGCCAGCATCGGCTGCAAATACGTCTATTTCAGCAACACAACAACCAGCTATCCAGCAACCGAATGTCTcc             >  1:100938/1‑81 (MQ=255)                                                              atatTCAGACGCCAGCATCGGCTGCAAATACGTCTATTTCAGCAACACAACAACCAGCTATCCAGCAACCGAATGTCTCCg            >  2:100932/1‑81 (MQ=255)                                                                atTCAGACGCCAGCATCGGCTGCAAATACGTCTATTTCAGCAACACAACAACCAGCTATCCAGCAACCGAATGTCTCCgg           >  1:100930/1‑80 (MQ=255)                                                                  tCAGACGCCAGCATCGGCTGCAAATACGTCTATTTCAGCAACACAACAACCAGCTATCCAGCAACCGAATGTCTCCGGTAc        >  2:100934/1‑81 (MQ=255)                                                                  tCAGACGCCAGCATCGGCTGCAAATACGTCTATTTCAGCAACACAACAACCAGCTATCCAGCAACCGAATGTCTCCGGTAc        >  2:100940/1‑81 (MQ=255)                                                                   cAGACGCCAGCATCGGCTGCAAATACGTCTATTTCAGCAACACAACAACCAGCTATCCAGCAACCGAATGTCTCCGGTAcc       >  1:100943/1‑81 (MQ=255)                                                                     gACGCCAGCATCGGCTGCAAATACGTCTATTTCAGCAACACAACAACCAGCTATCCAGCAACCGAATGTCTCCGGTACCGt     >  2:100925/1‑81 (MQ=255)                                                                         ccAGCATCGGCTGCAAATACGTCTATTTCAGCAACACAACAACCAGCTATCCAGCAACCGAATGTCTCCGGTACCGTCTg  >  2:100937/1‑80 (MQ=255)                                                                               |                                                                          CACATGGCCAGTGGTTTAGCGGTTGCGATTGCGTTGGCGGCTTGCGCAGATAAAAGCGCGGATATTCAGACGCCAGCACCGGCTGCAAATACGTCTATTTCAGCAACACAACAACCAGCTATCCAGCAACCGAATGTCTCCGGTACCGTCTG  >  NC_000913/475391‑475542

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑