Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	514,719	C→T	W47* (TGG→TGA)	ybbJ ←	NfeD‑like family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	514,719	0	C	T	100.0%	128.1 / NA	35	W47* (TGG→TGA)	ybbJ	NfeD‑like family protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (19/16);  total (19/16)

TTTCCACCACAGCCAGGCGGCGAGCAGCGTCAGGATGGCAAACATTACCCCTTGCCACTCCCAACCCAGCGGCACCAGCCAGACCACCAGGCCGGTAATCACCGCTGCCACGCCGCTCCACAACAAATAACCATTTCCGCCCAGCATCTCGGCTGC  >  NC_000913/514641‑514796                                                                               |                                                                              tttCCACCACAGCCAGGCGGCGAGCAGCGTCAGGATGGCAAACATTACCCCTTGCCACTCCCAACCCAGCGGCAccagtca                                                                             <  2:108923/81‑1 (MQ=255)          caGCCAGGCGGCGAGCAGCGTCAGGATGGCAAACATTACCCCTTGCCACTCCCAACCCAGCGGCACCAGTCAGACCACCAg                                                                    >  1:108982/1‑81 (MQ=255)             ccAGGCGGCGAGCAGCGTCAGGATGGCAAACATTACCCCTTGCCACTCCCAACCCAGCGGCACCAGTCAGACCACCAGGcc                                                                 <  2:108924/81‑1 (MQ=255)             ccAGGCGGCGAGCAGCGTCAGGATGGCAAACATTACCCCTTGCCACTCCCAACCCAGCGGCACCAGTCAGACCACCAGGcc                                                                 <  1:108925/81‑1 (MQ=255)               aGGCGGCGAGCAGCGTCAGGATGGCAAACATTACCCCTTGCCACTCCCAACCCAGCGGCACCAGTCAGACCACCAGGCCgg                                                               >  1:108989/1‑81 (MQ=255)               aGGCGGCGAGCAGCGTCAGGATGGCAAACATTACCCCTTGCCACTCCCAACCCAGCGGCACCAGTCAGACCACCAGGCCgg                                                               >  1:108985/1‑81 (MQ=255)               aGGCGGCGAGCAGCGTCAGGATGGCAAACATTACCCCTTGCCACTCCCAACCCAGCGGCACCAGTCAGACCACCAGGCCgg                                                               >  2:108986/1‑81 (MQ=255)               aGGCGGCGAGCAGCGTCAGGATGGCAAACATTACCCCTTGCCACTCCCAACCCAGCGGCACCAGTCAGACCACCAGGCCgg                                                               >  1:108967/1‑81 (MQ=255)                  cggcgAGCAGCGTCAGGATGGCAAACATTACCCCTTGCCACTCCCAACCCAGCGGCACCAGTCAGACCACCAGGCCGGTaa                                                            <  2:108926/81‑1 (MQ=255)                    gcgAGCAGCGTCAGGATGGCAAACATTACCCCTTGCCACTCCCAACCCAGCGGCACCAGTCAGACCACCAGGCCGGTAAt                                                           >  1:108998/1‑80 (MQ=255)                    gcgAGCAGCGTCAGGATGGCAAACATTACCCCTTGCCACTCCCAACCCAGCGGCACCAGTCAGACCACCAGGCCGGTAATc                                                          <  1:108927/81‑1 (MQ=255)                          agcGTCAGGATGGCAAACATTACCCCTTGCCACTCCCAACCCAGCGGCACCAGTCAGACCACCAGGCCGGTAATCACCGCt                                                    <  1:108928/81‑1 (MQ=255)                            cGTCAGGATGGCAAACATTACCCCTTGCCACTCCCAACCCAGCGGCACCAGTCAGACCACCAGGCCGGTAATCACCGCTGc                                                  >  2:108976/1‑81 (MQ=255)                             gTCAGGATGGCAAACATTACCCCTTGCCACTCCCAACCCAGCGGCACCAGTCAGACCACCAGGCCGGTAATCACCGCTGcc                                                 <  2:108929/81‑1 (MQ=255)                                aGGATGGCAAACATTACCCCTTGCCACTCCCAACCCAGCGGCACCAGTCAGACCACCAGGCCGGTAATCACCGCTGCCAcg                                              >  2:108993/1‑81 (MQ=255)                                aGGATGGCAAACATTACCCCTTGCCACTCCCAACCCAGCGGCACCAGTCAGACCACCAGGCCGGTAATCACCGCTGCCAc                                               <  2:108930/80‑1 (MQ=255)                                   aTGGCAAACATTACCCCTTGCCACTCCCAACCCAGCGGCACCAGTCAGACCACCAGGCCGGTAATCACCGCTGCCAcgccg                                           <  1:108931/81‑1 (MQ=255)                                    tGGCAAACATTACCCCTTGCCACTCCCAACCCAGCGGCACCAGTCAGACCACCAGGCCGGTAATCACCGCTGCCAcgccgc                                          <  1:108932/81‑1 (MQ=255)                                       cAAACATTACCCCTTGCCACTCCCAACCCAGCGGCACCAGTCAGACCACCAGGCCGGTAATCACCGCTGCCACGCCGCTcc                                       <  1:108934/81‑1 (MQ=255)                                       cAAACATTACCCCTTGCCACTCCCAACCCAGCGGCACCAGTCAGACCACCAGGCCGGTAATCACCGCTGCCACGCCGCTcc                                       <  1:108933/81‑1 (MQ=255)                                       cAAACATTACCCCTTGCCACTCCCAACCCAGCGGCACCAGTCAGACCACCAGGCCGGTAATCACCGCTGCCACGCCGCTcc                                       <  2:108935/81‑1 (MQ=255)                                         aaCATTACCCCTTGCCACTCCCAACCCAGCGGCACCAGTCAGACCACCAGGCCGGTAATCACCGCTGCCACGCCGCTCcac                                     <  2:108936/81‑1 (MQ=255)                                           cATTACCCCTTGCCACTCCCAACCCAGCGGCACCAGTCAGACCACCAGGCCGGTAATCACCGCTGCCACGCCGCTCCacaa                                   >  2:108999/1‑81 (MQ=255)                                              tACCCCTTGCCACTCCCAACCCAGCGGCACCAGTCAGACCACCAGGCCGGTAATCACCGCTGCCACGCCGCTCCacaaca                                 >  1:108983/1‑80 (MQ=255)                                                        cACTCCCAACCCAGCGGCACCAGTCAGACCACCAGGCCGGTAATCACCGCTGCCACGCCGCTCCACAACAAATAACCAttt                      <  2:108937/81‑1 (MQ=255)                                                           tCCCAACCCAGCGGCACCAGTCAGACCACCAGGCCGGTAATCACCGCTGCCACGCCGCTCCACAACAAATAACCATTTCCg                   >  1:109003/1‑81 (MQ=255)                                                            cccAACCCAGCGGCACCAGTCAGACCACCAGGCCGGTAATCACCGCTGCCACGCCGCTCCACAACAAATAACCATTTCCGc                  >  1:108994/1‑81 (MQ=255)                                                             ccAACCCAGCGGCACCAGTCAGACCACCAGGCCGGTAATCACCGCTGCCACGCCGCTCCACAACAAATAACCATTTCCGc                  <  2:108938/80‑1 (MQ=255)                                                                aCCCAGCGGCACCAGTCAGACCACCAGGCCGGTAATCACCGCTGCCACGCCGCTCCACAACAAATAACCATTTCCGCCCAg              >  1:109002/1‑81 (MQ=255)                                                                  ccAGCGGCACCAGTCAGACCACCAGGCCGGTAATCACCGCTGCCACGCCGCTCCACAACAAATAACCATTTCCGCCCAGCa            >  2:108990/1‑81 (MQ=255)                                                                   cAGCGGCACCAGTCAGACCACCAGGCCGGTAATCACCGCTGCCACGCCGCTCCACAACAAATAACCATTTCCGCCCAGCAt           >  1:108996/1‑81 (MQ=255)                                                                     gCGGCACCAGTCAGACCACCAGGCCGGTAATCACCGCTGCCACGCCGCTCCACAACAAATAACCATTTCCGCCCAGCAtct         >  2:108995/1‑81 (MQ=255)                                                                      cGGCACCAGTCAGACCACCAGGCCGGTAATCACCGCTGCCACGCCGCTCCACAACAAATAACCATTTCCGCCCAGCAtctc        >  1:108992/1‑81 (MQ=255)                                                                       ggCACCAGTCAGACCACCAGGCCGGTAATCACCGCTGCCACGCCGCTCCACAACAAATAACCATTTCCGCCCAGCATCTCg       >  2:108981/1‑81 (MQ=255)                                                                       ggCACCAGTCAGACCACCAGGCCGGTAATCACCGCTGCCACGCCGCTCCACAACAAATAACCATTTCCGCCCAGCATCTCg       >  2:108984/1‑81 (MQ=255)                                                                          aCCAGTCAGACCACCAGGCCGGTAATCACCGCTGCCACGCCGCTCCACAACAAATAACCATTTCCGCCCAGCATCTCGGCt    <  1:108939/81‑1 (MQ=255)                                                                            cagtcagACCACCAGGCCGGTAATCACCGCTGCCACGCCGCTCCACAACAAATATCCATTTCCGCCCAGCATCTCGGCTGc  >  1:108980/1‑81 (MQ=255)                                                                               |                                                                              TTTCCACCACAGCCAGGCGGCGAGCAGCGTCAGGATGGCAAACATTACCCCTTGCCACTCCCAACCCAGCGGCACCAGCCAGACCACCAGGCCGGTAATCACCGCTGCCACGCCGCTCCACAACAAATAACCATTTCCGCCCAGCATCTCGGCTGC  >  NC_000913/514641‑514796

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑