Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	548,605	G→A	E83E (GAG→GAA)	ylbE →	DUF1116 domain‑containing protein YlbE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	548,605	0	G	A	100.0%	121.7 / NA	34	E83E (GAG→GAA)	ylbE	DUF1116 domain‑containing protein YlbE
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (20/14);  total (20/14)

TGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAGT  >  NC_000913/548529‑548680                                                                             |                                                                            tGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAACAgg                                                                         <  1:115483/81‑1 (MQ=255)  gAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAACAggg                                                                        >  2:115519/1‑81 (MQ=255)     aGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAACAGGGGGa                                                                     <  2:115484/81‑1 (MQ=255)           gtgcgtgGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCACTACTGGAACAGGGGGAAGTGaa                                                               >  2:115527/1‑81 (MQ=255)            tgcgtgGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAACAGGGGGAAGTGAAc                                                              <  2:115485/81‑1 (MQ=255)             gcgtgGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAACAGGGGGAAGTGAACt                                                             >  2:115518/1‑81 (MQ=255)              cgtgGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAACAGGGGGAAGTGAACtt                                                            >  1:115535/1‑81 (MQ=255)              cgtgGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAACAGGGGGAAGTGAACt                                                             <  2:115486/80‑1 (MQ=255)               gtgGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAACAGGGGGAAGTGAACtt                                                            >  2:115534/1‑80 (MQ=255)               gtgGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAACAGGGGGAAGTGAACtt                                                            >  1:115520/1‑80 (MQ=255)                 gggCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAACAGGGGGAAGTGAACTTCAt                                                         >  1:115539/1‑81 (MQ=255)                      cATGTCTGTTCGAAGGTTGGGCGAAAGGTGAAGCGCAGGCGCTGGCAATACTGGAACAGGGGGAAGTGAACTTCATTCCtt                                                    <  2:115488/81‑1 (MQ=255)                      cATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAACAGGGGGAAGTGAACTTCATTCCtt                                                    <  2:115487/81‑1 (MQ=255)                          tCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAACAGGGGGAAGTGAACTTCATTCCTTGTc                                                 <  1:115489/80‑1 (MQ=255)                             gTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAACAGGGGGAAGTGAACTTCATTCCTTGTCACCa                                             >  1:115529/1‑81 (MQ=255)                             gTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAACAGGGGGAAGTGAACTTCATTCCTTGTCACCa                                             >  2:115533/1‑81 (MQ=255)                                        gggCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAACAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGcc                                   <  2:115490/80‑1 (MQ=255)                                          gCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAACAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTc                                <  1:115491/81‑1 (MQ=255)                                            gAAAGATGAAGCGCAGGCGCTGGCAATACTGGAACAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCgg                              >  1:115528/1‑81 (MQ=255)                                              aaGATGAAGCGCAGGCGCTGGCAATACTGGAACAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGc                            >  1:115547/1‑81 (MQ=255)                                              aaGATGAAGCGCAGGCGCTGGCAATACTGGAACAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGc                            <  2:115492/81‑1 (MQ=255)                                               aGATGAAGCGCAGGCGCTGGCAATACTGGAACAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGcc                           <  2:115493/81‑1 (MQ=255)                                               aGATGAAGCGCAGGCGCTGGCAATACTGGAACAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGc                            >  2:115526/1‑80 (MQ=255)                                                 aTGAAGCGCAGGCGCTGGCAATACTGGAACAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCaa                         >  1:115525/1‑81 (MQ=255)                                                       cgcAGGCGCTGGCAATACTGGAACAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCg                   >  1:115532/1‑81 (MQ=255)                                                          aGGCGCTGGCAATACTGGAACAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTa                >  1:115545/1‑81 (MQ=255)                                                              gcTGGCAATACTGGAACAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTAc            >  1:115537/1‑81 (MQ=255)                                                                 ggCAATACTGGAACAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTc         >  1:115554/1‑81 (MQ=255)                                                                 ggCAATACTGGAACAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTc         >  2:115552/1‑81 (MQ=255)                                                                  gCAATACTGGAACAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTc         <  1:115494/80‑1 (MQ=255)                                                                   cAATACTGGAACAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTg       >  1:115549/1‑81 (MQ=255)                                                                        cTGGAACAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAg   <  2:115495/80‑1 (MQ=255)                                                                        cTGGAACAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAg   >  2:115540/1‑80 (MQ=255)                                                                        cTGGAACAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAGt  <  2:115496/81‑1 (MQ=255)                                                                             |                                                                            TGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAGT  >  NC_000913/548529‑548680

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑