| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 551,066 | G→T | A210S (GCC→TCC) | ybcF → | putative carbamate kinase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 551,066 | 0 | G | T | 97.1% | 118.2 / NA | 34 | A210S (GCC→TCC) | ybcF | putative carbamate kinase |
| Reads supporting (aligned to +/- strand): ref base G (0/0); major base T (16/17); minor base A (1/0); total (17/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTGATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGTATATGAAAACTGGGGAACGCCG > NC_000913/550988‑551140 | ggcgggggTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTGATTGATAAAGATCTCGCCGCTGCGTTGCTCAcc > 2:116114/1‑81 (MQ=255)ggcggcggTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTGATTGATAAAGATCTCGCCGCTGCGTTGCTCTcc > 1:116107/1‑81 (MQ=255) gcggcggTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTGATTGATAAAGATCTCGCCGCTGCGTTGCTCTCCg < 2:116067/81‑1 (MQ=255) gcggcggTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTGATTGATAAAGATCTCGCCGCTGCGTTGCTCTCCg < 1:116066/81‑1 (MQ=255) ggTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTGATTGATAAAGATCTCGCCGCTGCGTTGCTCTCCGAg > 1:116112/1‑78 (MQ=255) ggTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTGATTGATAAAGATCTCGCCGCTGCGTTGCTCTCCGAg > 1:116108/1‑78 (MQ=255) tgtgCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTGATTGATAAAGATCTCGCCGCTGCGTTGCTCTCCGAGCAGAt > 2:116113/1‑81 (MQ=255) acggatgacggaGCAGGGAGTGAAGCAGTGATTGATAAAGATCTCGCCGCTGCGTTGCTCTCCGAGCAGATTAATGCAGAt < 1:116068/81‑1 (MQ=255) acggatgacggaGCAGGGAGTGAAGCAGTGATTGATAAAGATCTCGCCGCTGCGTTGCTCTCCGAGCAGATTAATGCAGAt < 1:116069/81‑1 (MQ=255) ggatgacggaGCAGGGAGTGAAGCAGTGATTGATAAAGATCTCGCCGCTGCGTTGCTCTCCGAGCAGATTAATGCAGATgg < 2:116070/81‑1 (MQ=255) gatgacggaGCAGGGAGTGAAGCAGTGATTGATAAAGATCTCGCCGCTGCGTTGCTCTCCGAGCAGATTAATGCAGATGGa > 2:116100/1‑81 (MQ=255) gatgacggaGCAGGGAGTGAAGCAGTGATTGATAAAGATCTCGCCGCTGCGTTGCTCTCCGAGCAGATTAATGCAGATGGa > 1:116122/1‑81 (MQ=255) atgacggaGCAGGGAGTGAAGCAGTGATTGATAAAGATCTCGCCGCTGCGTTGCTCTCCGAGCAGATTAATGCAGATGGAc > 1:116116/1‑81 (MQ=255) gacggaGCAGGGAGTGAAGCAGTGATTGATAAAGATCTCGCCGCTGCGTTGCTCTCCGAGCAGATTAATGCAGATGGACTg > 1:116109/1‑81 (MQ=255) cggaGCAGGGAGTGAAGCAGTGATTGATAAAGATCTCGCCGCTGCGTTGCTCTCCGAGCAGATTAATGCAGATGGACTGGt < 2:116071/81‑1 (MQ=255) ggaGCAGGGAGTGAAGCAGTGATTGATAAAGATCTCGCCGCTGCGTTGCTCTCCGAGCAGATTAATGCAGATGGACTGGt > 1:116118/1‑80 (MQ=255) gaGCAGGGAGTGAAGCAGTGATTGATAAAGATCTCGCCGCTGCGTTGCTCTCCGAGCAGATTAATGCAGATGGACTGGTGa > 2:116105/1‑81 (MQ=255) gggAGTGAAGCAGTGATTGATAAAGATCTCGCCGCTGCGTTGCTCTCCGAGCAGATTAATGCAGATGGACTGGTGATCCTc < 1:116072/81‑1 (MQ=255) aaGCAGTGATTGATAAAGATCTCGCCGCTGCGTTGCTCTCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGAt < 1:116073/80‑1 (MQ=255) aaGCAGTGATTGATAAAGATCTCGCCGCTGCGTTGCTCTCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGAt < 1:116074/80‑1 (MQ=255) gCAGTGATTGATAAAGATCTCGCCGCTGCGTTGCTCTCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCt > 1:116111/1‑81 (MQ=255) gCAGTGATTGATAAAGATCTCGCCGCTGCGTTGCTCTCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCt < 1:116075/81‑1 (MQ=255) cAGTGATTGATAAAGATCTCGCCGCTGCGTTGCTCTCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCt > 1:116130/1‑80 (MQ=255) cAGTGATTGATAAAGATCTCGCCGCTGCGTTGCTCTCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTg < 1:116076/81‑1 (MQ=255) cAGTGATTGATAAAGATCTCGCCGCTGCGTTGCTCTCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTg < 1:116077/81‑1 (MQ=255) aGTGATTGATAAAGATCTCGCCGCTGCGTTGCTCTCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGa < 1:116078/81‑1 (MQ=255) attgatAAAGATCTCGCCGCTGCGTTGCTCTCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCg > 2:116119/1‑81 (MQ=255) tAAAGATCTCGCCGCTGCGTTGCTCTCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGtata < 2:116079/81‑1 (MQ=255) gccgcTGCGTTGCTCTCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGTATATGAAAACTgg > 1:116131/1‑81 (MQ=255) gccgcTGCGTTGCTCTCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGTATATGAAAACTg < 1:116080/80‑1 (MQ=255) cgcTGCGTTGCTCTCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGTATATGAAAACTgggg < 2:116081/81‑1 (MQ=255) cGTTGCTCTCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGTATATGAAAACTGGGGAAcgc < 2:116082/81‑1 (MQ=255) gCTCTCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGTATATGAAAACTGGGGAAcgccg > 1:116115/1‑79 (MQ=255) gCTCTCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGTATATGAAAACTGGGGAAcgccg > 2:116120/1‑79 (MQ=255) | GGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTGATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGTATATGAAAACTGGGGAACGCCG > NC_000913/550988‑551140 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |