Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	584,131	C→A	R151R (CGG→AGG)	appY →	DNA‑binding transcriptional activator AppY

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	584,131	0	C	A	100.0%	39.8 / NA	12	R151R (CGG→AGG)	appY	DNA‑binding transcriptional activator AppY
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (6/6);  total (6/6)

TAAATTCATTTATACTAACAAATATAAATACATTTACCTGTAAAATTACTGGAATAATCAGTTTTAATATTGAGCGGCAATGGCATTTAAAAGATATTGCGGAATTGATTTATACGAGTGAAAGTTTAATAAAAAAAAGATTAAGGGATGA  >  NC_000913/584057‑584207                                                                           |                                                                             tAAATTCATTTATACTAACAAATATAAATACATTTACCTGTAAAATTACTGGAATAATCAGTTTTAATATTGAGAGGCAAt                                                                        <  1:121649/81‑1 (MQ=255) tAAATTCATTTATACTAACAAATATAAATACATTTACCTGTAAAATTACTGGAATAATCAGTTTTAATATTGAGAGGCAAt                                                                        <  2:121650/81‑1 (MQ=255)           tataCTAACAAATATAAATACATTTACCTGTAAAATTACTGGAATAATCAGTTTTAATATTGAGAGGCAATGGCATTTaa                                                               >  2:121666/1‑80 (MQ=255)               cTAACAAATATAAATACATTTACCTGTAAAATTACTGGAATAATCAGTTTTAATATTGAGAGGCAATGGCATTTAAAAGat                                                          <  1:121651/81‑1 (MQ=255)                 aaCAAATATAAATACATTTACCTGTAAAATTACTGGAATAATCAGTTTTAATATTGAGAGGCAATGGCATTTAAAAGatat                                                        >  1:121668/1‑81 (MQ=255)                            aTACATTTACCTGTAAAATTACTGGAATAATCAGTTTTAATATTGAGAGGCAATGGCATTTAAAAGATATTGCGGAATTGa                                             <  1:121652/81‑1 (MQ=255)                                           aaaTTACTGGAATAATCAGTTTTAATATTGAGAGGCAATGGCATTTAAAAGATATTGCGGAATTGATTTATACGAGTGaaa                              <  1:121653/81‑1 (MQ=255)                                                  tGGAATAATCAGTTTTAATATTGAGAGGCAATGGCATTTAAAAGATATTGCGGAATTGATTTATACGAGTGAAAGTTtaa                        >  1:121673/1‑80 (MQ=255)                                                       taatCAGTTTTAATATTGAGAGGCAATGGCATTTAAAAGATATTGCGGAATTGATTTATACGAGTGAAAGTTTAATaaaaa                  <  1:121654/81‑1 (MQ=255)                                                           cAGTTTTAATATTGAGAGGCAATGGCATTTAAAAGATATTGCGGAATTGATTTATACGAGTGAAAGTTTAATaaaaaa                 >  1:121684/1‑78 (MQ=255)                                                               tttAATATTGAGAGGCAATGGCATTTAAAAGATATTGCGGAATTGATTTATACGAGTGAAAGTTTAATAAAAAAAAGAtt           >  1:121675/1‑80 (MQ=255)                                                                     atTGAGAGGCAATGGCATTTAAAAGATATTGCGGAATTGATTTATACGAGTGAAAGTTTAATAAAAAAAAGATTAAGGGAt    >  2:121670/1‑81 (MQ=255)                                                                        gagaGGCAATGGCATTTAAAAGATATTGCGGAATTGATTTATACGAGTGAAAGTTTAATAAAAAAAAGATTAAGGGATGa  <  1:121655/80‑1 (MQ=255)                                                                           |                                                                             TAAATTCATTTATACTAACAAATATAAATACATTTACCTGTAAAATTACTGGAATAATCAGTTTTAATATTGAGCGGCAATGGCATTTAAAAGATATTGCGGAATTGATTTATACGAGTGAAAGTTTAATAAAAAAAAGATTAAGGGATGA  >  NC_000913/584057‑584207

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑