Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	589,509	G→A	A482A (GCC→GCT)	nfrA ←	bacteriophage N4 receptor, outer membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	589,509	0	G	A	100.0%	143.3 / NA	39	A482A (GCC→GCT)	nfrA	bacteriophage N4 receptor, outer membrane protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (16/23);  total (16/23)

TGTTCGGCCTGAAGCCATGCATACAACGCCACACCGGGTAGCGTGTCCCGATAACACTTTGCCAGACGGTTCCAGGCGGCGGCATCGTAGGAAGGCGACATATCGCCCAGCAAGCGAACTATTGCCGGGCAATTATCTGCAATACCCGGCAA  >  NC_000913/589435‑589586                                                                           |                                                                              tgttCGGCCTGAAGCCATGCATACAACGCCACACCGGGTAGCGTGTCCCGATAACACTTTGCCAGACGGTTCCCagcggcg                                                                         >  1:122607/1‑81 (MQ=255)  gttCGGCCTGAAGCCATGCATACAACGCCACACCGGGTAGCGTGTCCCGATAACACTTTGCCAGACGGTTCCAagcggcg                                                                         <  2:122544/80‑1 (MQ=255)  gttCGGCCTGAAGCCATGCATACAACGCCACACCGGGTAGCGTGTCCCGATAACACTTTGCCAGACGGTTCCAagcggcg                                                                         <  2:122543/80‑1 (MQ=255)      ggCCTGAAGCCATGCATACAACGCCACACCGGGTAGCGTGTCCCGATAACACTTTGCCAGACGGTTCCAAGCGGCGGCATc                                                                    <  1:122546/81‑1 (MQ=255)      ggCCTGAAGCCATGCATACAACGCCACACCGGGTAGCGTGTCCCGATAACACTTTGCCAGACGGTTCCAAGCGGCGGCATc                                                                    <  2:122545/81‑1 (MQ=255)          tGAAGCCATGCATACAACGCCACACCGGGTAGCGTGTCCCGATAACACTTTGCCAGACGGTTCCAAGCGGCGGCATCGTAg                                                                >  2:122593/1‑81 (MQ=255)            aaGCCATGCATACAACGCCACACCGGGTAGCGTGTCCCGATAACACTTTGCCAGACGGTTCCAAGCGGCGGCATCGTAg                                                                <  2:122548/79‑1 (MQ=255)            aaGCCATGCATACAACGCCACACCGGGTAGCGTGTCCCGATAACACTTTGCCAGACGGTTCCAAGCGGCGGCATCGTAGGa                                                              <  1:122547/81‑1 (MQ=255)                 aTGCATACAACGCCACACCGGGTAGCGTGTCCCGATAACACTTTGCCAGACGGTTCCAAGCGGCGGCATCGTAGGAAGGCg                                                         <  1:122549/81‑1 (MQ=255)                 aTGCATACAACGCCACACCGGGTAGCGTGTCCCGATAACACTTTGCCAGACGGTTCCAAGCGGCGGCATCGTAGGAAGGCg                                                         <  2:122550/81‑1 (MQ=255)                   gCATACAACGCCACACCGGGTAGCGTGTCCCGATAACACTTTGCCAGACGGTTCCAAGCGGCGGCATCGTAGGAAGGCGa                                                        >  1:122601/1‑80 (MQ=255)                   gCATACAACGCCACACCGGGTAGCGTGTCCCGATAACACTTTGCCAGACGGTTCCAAGCGGCGGCATCGTAGGAAGGCGa                                                        >  2:122603/1‑80 (MQ=255)                    cATACAACGCCACACCGGGTAGCGTGTCCCGATAACACTTTGCCAGACGGTTCCAAGCGGCGGCATCGTAGGAAGGCGAc                                                       <  1:122551/80‑1 (MQ=255)                      tACAACGCCACACCGGGTAGCGTGTCCCGATAACACTTTGCCAGACGGTTCCAAGCGGCGGCATCGTAGGAAGGCGACata                                                    <  1:122552/81‑1 (MQ=255)                         aaCGCCACACCGGGTAGCGTGTCCCGATAACACTTTGCCAGACGGTTCCAAGCGGCGGCATCGTAGGAAGGCGACATATCg                                                 >  1:122609/1‑81 (MQ=255)                         aaCGCCACACCGGGTAGCGTGTCCCGATAACACTTTGCCAGACGGTTCCAAGCGGCGGCATCGTAGGAAGGCGACATATCg                                                 <  1:122553/81‑1 (MQ=255)                            gCCACACCGGGTAGCGTGTCCCGATAACACTTTGCCAGACGGTTCCAAGCGGCGGCATCGTAGGAAGGCGACATATCGccc                                              <  1:122554/81‑1 (MQ=255)                               acacCGGGTAGCGTGTCCCGATAACACTTTGCCAGACGGTTCCAAGCGGCGGCATCGTAGGAAGGCGACATATCGCCCAGc                                           <  2:122555/81‑1 (MQ=255)                                cacCGGGTAGCGTGTCCCGATAACACTTTGCCAGACGGTTCCAAGCGGCGGCATCGTAGGAAGGCGACATATCGCCCAGc                                           >  1:122605/1‑80 (MQ=255)                                 acCGGGTAGCGTGTCCCGATAACACTTTGCCAGACGGTTCCAAGCGGCGGCATCGTAGGAAGGCGACATATCGCCCAGCaa                                         <  1:122556/81‑1 (MQ=255)                                      gTAGCGTGTCCCGATAACACTTTGCCAGACGGTTCCAAGCGGCGGCATCGTAGGAAGGCGACATATCGCCCAGCAAGCGaa                                    >  2:122606/1‑81 (MQ=255)                                          cGTGTCCCGATAACACTTTGCCAGACGGTTCCAAGCGGCGGCATCGTAGGAAGGCGACATATCGCCCAGCAAGCGAACTAt                                >  1:122608/1‑81 (MQ=255)                                           gtgtCCCGATAACACTTTGCCAGACGGTTCCAAGCGGCGGCATCGTAGGAAGGCGACATATCGCCCAGCAAGCGAACTAtt                               >  2:122604/1‑81 (MQ=255)                                           gtgtCCCGATAACACTTTGCCAGACGGTTCCAAGCGGCGGCATCGTAGGAAGGCGACATATCGCCCAGCAAGCGAACTAtt                               <  2:122558/81‑1 (MQ=255)                                           gtgtCCCGATAACACTTTGCCAGACGGTTCCAAGCGGCGGCATCGTAGGAAGGCGACATATCGCCCAGCAAGCGAACTAtt                               <  2:122557/81‑1 (MQ=255)                                            tgtCCCGATAACACTTTGCCAGACGGTTCCAAGCGGCGGCATCGTAGGAAGGCGACATATCGCCCAGCAAGCGAACTATTg                              >  2:122613/1‑81 (MQ=255)                                               cccGATAACACTTTGCCAGACGGTTCCAAGCGGCGGCATCGTAGGAAGGCGACATATCGCCCAGCAAGCGAACTATTGCCg                           >  2:122610/1‑81 (MQ=255)                                                ccGATAACACTTTGCCAGACGGTTCCAAGCGGCGGCATCGTAGGAAGGCGACATATCGCCCAGCAAGCGAACTATTGCCg                           <  2:122559/80‑1 (MQ=255)                                                  gATAACACTTTGCCAGACGGTTCCAAGCGGCGGCATCGTAGGAAGGCGACATATCGCCCAGCAAGCGAACTATTGCCggg                         >  1:122618/1‑80 (MQ=255)                                                         cTTTGCCAGACGGTTCCAAGCGGCGGCATCGTAGGAAGGCGACATATCGCCCAGCAAGCGAACTATTGCCGGGCAATTATc                 <  2:122560/81‑1 (MQ=255)                                                           ttGCCAGACGGTTCCAATCGGCGGCATCGTAGGAAGGCGACATATCGCCCAGCAAGCGAACTATTGCCGGGCAATTATCTg               >  1:122616/1‑81 (MQ=255)                                                             gCCAGACGGTTCCAAGCGGCGGCATCGTAGGAAGGCGACATATCGCCCAGCAAGCGAACTATTGCCGGGCAATTATCTGc              <  2:122561/80‑1 (MQ=255)                                                              ccAGACGGTTCCAAGCGGCGGCATCGTAGGAAGGCGACATATCGCCCAGCAAGCGAACTATTGCCGGGCAATTATCTGCaa            <  2:122562/81‑1 (MQ=255)                                                               cAGACGGTTCCAAGCGGCGGCATCGTAGGAAGGCGACATATCGCCCAGCAAGCGAACTATTGCCGGGCAATTATCTGCaa            >  1:122615/1‑80 (MQ=255)                                                               cAGACGGTTCCAAGCGGCGGCATCGTAGGAAGGCGACATATCGCCCAGCAAGCGAACTATTGCCGGGCAATTATCTGCAAt           <  2:122564/81‑1 (MQ=255)                                                               cAGACGGTTCCAAGCGGCGGCATCGTAGGAAGGCGACATATCGCCCAGCAAGCGAACTATTGCCGGGCAATTATCTGCAAt           <  1:122563/81‑1 (MQ=255)                                                                 gACGGTTCCAAGCGGCGGCATCGTAGGAAGGCGACATATCGCCCAGCAAGCGAACTATTGCCGGGCAATTATCTGCAATAc         <  1:122565/81‑1 (MQ=255)                                                                   cGGTTCCAAGCGGCGGCATCGTAGGAAGGCGACATATCGCCCAGCAAGCGAACTATTGCCGGGCAATTATCTGCAATAccc       <  1:122566/81‑1 (MQ=255)                                                                    ggTTCCAAGCGGCGGCATCGTAGGAAGGCGACATATCGCCCAGCAAGCGAACTATTGCCGGGCAATTATCTGCAATACCCg      >  1:122619/1‑81 (MQ=255)                                                                    ggTTCCAAGCGGCGGCATCGTAGGAAGGCGACATATCGCCCAGCAAGCGAACTATTGCCGGGCAATTATCTGCAATACCCg      >  2:122611/1‑81 (MQ=255)                                                                     gTTCCAAGCGGCGGCATCGTAGGAAGGCGACATATCGCCCAGCAAGCGAACTATTGCCGGGCAATTATCTGCAATACCCgg     <  2:122567/81‑1 (MQ=255)                                                                        ccAAGCGGCGGCATCGTAGGAAGGCGACATATCGCCCAGCAAGCGAACTATTGCCGGGCAATTATCTGCAATACCCGGCaa  >  1:122614/1‑81 (MQ=255)                                                                           |                                                                              TGTTCGGCCTGAAGCCATGCATACAACGCCACACCGGGTAGCGTGTCCCGATAACACTTTGCCAGACGGTTCCAGGCGGCGGCATCGTAGGAAGGCGACATATCGCCCAGCAAGCGAACTATTGCCGGGCAATTATCTGCAATACCCGGCAA  >  NC_000913/589435‑589586

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑