Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	602,184	G→A	E76K (GAA→AAA)	pheP →	phenylalanine:H(+) symporter PheP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	602,184	0	G	A	100.0%	123.6 / NA	34	E76K (GAA→AAA)	pheP	phenylalanine:H(+) symporter PheP
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (15/19);  total (15/19)

GGGTCCGGCTGTATTGCTGGGCTACGGCGTCGCCGGGATCATCGCTTTCCTGATTATGCGCCAGCTTGGCGAAATGGTGGTTGAGGAGCCGGTATCCGGTTCATTTGCCCACTTTGCCTATAAATACTGGGGACCGTTTGCGGGCTTC  >  NC_000913/602114‑602261                                                                       |                                                                              gggTCCGGCTGTATTGCTGGGCTACGGCGTCGCCGGGATCATCGCTTTCCTGATTATGCGCCAGCTTGGCAAAATggtggt                                                                     <  2:125179/81‑1 (MQ=255)  ggTCCGGCTGTATTGCTGGGCTACGGCGTCGCCGGGATCATCGCTTTCCTGATTATGCGCCAGCTTGGCAAAATGGTGGtt                                                                    >  1:125218/1‑81 (MQ=255)    tCCGGCTGTATTGCTGGGCTACGGCGTCGCCGGGATCATCGCTTTCCTGATTATGCGCCAGCTTGGCAAAATGGTGGTTga                                                                  <  2:125180/81‑1 (MQ=255)     ccGGCTGTATTGCTGGGCTACGGCGTCGCCGGGATCATCGCTTTCCTGATTATGCGCCAGCTTGGCAAAATGGTGGTTgag                                                                 >  1:125208/1‑81 (MQ=255)      cGGCTGTATTGCTGGGCTACGGCGTCGCCGGGATCATCGCTTTCCTGATTATGCGCCAGCTTGGCAAAATGGTGGTTgag                                                                 <  2:125181/80‑1 (MQ=255)       ggCTGTATTGCTGGGCTACGGCGTCGCCGGGATCATCGCTTTCCTGATTATGCGCCAGCTTGGCAAAATGGTGGTTgag                                                                 >  1:125214/1‑79 (MQ=255)           gTATTGCTGGGCTACGGCGTCGCCGGGATCATCGCTTTCCTGATTATGCGCCAGCTTGGCAAAATGGTGGTTGAGGAGCCg                                                           >  2:125215/1‑81 (MQ=255)            tATTGCTGGGCTACGGCGTCGCCGGGATCATCGCTTTCCTGATTATGCGCCAGCTTGGCAAAATGGTGGTTGAGGAGcc                                                            <  1:125182/79‑1 (MQ=255)             aTTGCTGGGCTACGGCGTCGCCGGGATCATCGCTTTCCTGATTATGCGCCAGCTTGGCAAAATGGTGGTTGAGGAGCCgg                                                          >  2:125213/1‑80 (MQ=255)              ttGCTGGGCTACGGCGTCGCCGGGATCATCGCTTTCCTGATTATGCGCCAGCTTGGCAAAATGGTGGTTGAGGAGCCGGt                                                         <  1:125183/80‑1 (MQ=255)                 cTGGGCTACGGCGTCGCCGGGATCATCGCTTTCCTGATTATGCGCCAGCTTGGCAAAATGGTGGTTGAGGAGCCGGTATcc                                                     <  2:125184/81‑1 (MQ=255)                           gCGTCGCCGGGATCATCGCTTTCCTGATTATGCGCCAGCTTGGCAAAATGGTGGTTGAGGAGCCGGTATCCGGTTCATTTg                                           <  1:125185/81‑1 (MQ=255)                            cGTCGCCGGGATCATCGCTTTCCTGATTATGCGCCAGCTTGGCAAAATGGTGGTTGAGGAGCCGGTATCCGGTTCATTTGc                                          >  1:125222/1‑81 (MQ=255)                             gTCGCCGGGATCATCGCTTTCCTGATTATGCGCCAGCTTGGCAAAATGGTGGTTGAGGAGCCGGTATCCGGTTCATTTGcc                                         >  1:125224/1‑81 (MQ=255)                                gCCGGGATCATCGCTTTCCTGATTATGCGCCAGCTTGGCAAAATGGTGGTTGAGGAGCCGGTATCCGGTTCATTTGCCCAc                                      <  2:125186/81‑1 (MQ=255)                                 ccGGGATCATCGCTTTCCTGATTATGCGCCAGCTTGGCAAAATGGTGGTTGAGGAGCCGGTATCCGGTTCATTTGCCCACt                                     <  1:125187/81‑1 (MQ=255)                                  cGGGATCATCGCTTTCCTGATTATGCGCCAGCTTGGCAAAATGGTGGTTGAGGAGCCGGTATCCGGTTCATTTGCCCACtt                                    <  1:125188/81‑1 (MQ=255)                                   gggATCATCGCTTTCCTGATTATGCGCCAGCTTGGCAAAATGGTGGTTGAGGAGCCGGTATCCGGTTCATTTGCCCACttt                                   <  1:125189/81‑1 (MQ=255)                                    ggATCATCGCTTTCCTGATTATGCGCCAGCTTGGCAAAATGGTGGTTGAGGAGCCGGTATCCGGTTCATTTGCCCACTTTg                                  <  2:125190/81‑1 (MQ=255)                                     gATCATCGCTTTCCTGATTATGCGCCAGCTTGGCAAAATGGTGGTTGAGGAGCCGGTATCCGGTTCATTTGCCCACTTTGc                                 >  1:125191/1‑81 (MQ=255)                                       tcatcGCTTTCCTGATTATGCGCCAGCTTGGCAAAATGGTGGTTGAGGAGCCGGTATCCGGTTCATTTGCCCACTTTGCCt                               <  1:125192/81‑1 (MQ=255)                                         atcGCTTTCCTGATTATGCGCCAGCTTGGCAAAATGGTGGTTGAGGAGCCGGTATCCGGTTCATTTGCCCACTTTGCCtat                             <  2:125193/81‑1 (MQ=255)                                           cGCTTTCCTGATTATGCGCCAGCTTGGCAAAATGGTGGTTGAGGAGCCGGTATCCGGTTCATTTGCCCACTTTGCCtata                            >  2:125227/1‑80 (MQ=255)                                            gCTTTCCTGATTATGCGCCAGCTTGGCAAAATGGTGGTTGAGGAGCCGGTATCCGGTTCATTTGCCCACTTTGCCTATaa                           <  1:125194/80‑1 (MQ=255)                                              tttCCTGATTATGCGCCAGCTTGGCAAAATGGTGGTTGAGGAGCCGGTATCCGGTTCATTTGCCCACTTTGCCTATAAATa                        >  1:125229/1‑81 (MQ=255)                                                tCCTGATTATGCGCCAGCTTGGCAAAATGGTGGTTGAGGTGCCGGTATCCGGTTCATTTGCCCACTTTGCCTATAAATAc                       <  1:125195/80‑1 (MQ=255)                                                  cTGATTATGCGCCAGCTTGGCAAAATGGTGGTTGAGGAGCCGGTATCCGGTTCATTTGCCCACTTTGCCTATAAATACTgg                    >  1:125220/1‑81 (MQ=255)                                                    gATTATGCGCCAGCTTGGCAAAATGGTGGTTGAGGAGCCGGTATCCGGTTCATTTGCCCACTTTGCCTATAAATACTggg                   >  1:125232/1‑80 (MQ=255)                                                     aTTATGCGCCAGCTTGGCAAAATGGTGGTTGAGGAGCCGGTATCCGGTTCATTTGCCCACTTTGCCTATAAATACTGGGGa                 >  1:125223/1‑81 (MQ=255)                                                     aTTATGCGCCAGCTTGGCAAAATGGTGGTTGAGGAGACGGTATCCGGTTCATTTGCCCACTTTGCCTATAAATACTggg                   <  2:125196/79‑1 (MQ=255)                                                         tGCGCCAGCTTGGCAAAATGGTGGTTGAGGAGCCGGTATCCGGTTCATTTGCCCACTTTGCCTATAAATACTGGGGACCGt             <  2:125197/81‑1 (MQ=255)                                                          gcgcCAGCTTGGCAAAATGGTGGTTGAGGAGCCGGTATCCGGTTCATTTGCCCACTTTGCCTATAAATACTGGGGACCGtt            >  1:125228/1‑81 (MQ=255)                                                           cgcCAGCTTGGCAAAATGGTGGTTGAGGAGCCGGTATCCGGTTCATTTGCCCACTTTGCCTATAAATACTGGGGACCGttt           <  1:125198/81‑1 (MQ=255)                                                                    ggCAAAATGGTGGTTGAGGAGCCGGTATCCGGTTCATTTGCCCACTTTGCCTATAAATACTGGGGACCGTTTGCGGGCTTc  >  1:125225/1‑81 (MQ=255)                                                                       |                                                                              GGGTCCGGCTGTATTGCTGGGCTACGGCGTCGCCGGGATCATCGCTTTCCTGATTATGCGCCAGCTTGGCGAAATGGTGGTTGAGGAGCCGGTATCCGGTTCATTTGCCCACTTTGCCTATAAATACTGGGGACCGTTTGCGGGCTTC  >  NC_000913/602114‑602261

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑