Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 649,472 G→A Q34* (CAA→TAA)  citF ← citrate lyase alpha subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913649,4720GA100.0% 141.0 / NA 39Q34* (CAA→TAA) citFcitrate lyase alpha subunit
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (16/23);  total (16/23)

TAAACCAGAGCGACGAATCGCTTCTTCCAGGTTGGCGCACAGTTTGCGATCGCGCGCTTTTTCAGCCTGGTAGGTTTGCTTTGGCGAGTTCTGGAAAGCGGCAAGATCGCATTCAGCGCGACGATTCCAGGCCGCTACCCGTTCTTGTCGTT  >  NC_000913/649395‑649546
                                                                             |                                                                          
tAAACCAGAGCGACGAATCGCTTCTTCCAGGTTGGCGCACAGTTTGCGATCGCGCGCTTTTTCAGCCTGGTAGGTTTACtt                                                                         <  2:134850/81‑1 (MQ=255)
tAAACCAGAGCGACGAATCGCTTCTTCCAGGTTGGCGCACAGTTTGCGATCGCGCGCTTTTTCAGCCTGGTAGGTTTACtt                                                                         <  1:134849/81‑1 (MQ=255)
    ccAGAGCGACGAATCGCTTCTTCCAGGTTGGCGCACAGTTTGCGATCGCGCGCTTTTTCAGCCTGGTAGGTTTACTTTGGc                                                                     <  1:134851/81‑1 (MQ=255)
      agagCGACGAATCGCTTCTTCCAGGTTGGCGCACAGTTTGCGATCGCGCGCTTTTTCAGCCTGGTAGGTTTACTTTGGc                                                                     >  2:134909/1‑79 (MQ=255)
        agCGACGAATCGCTTCTTCCAGGTTGGCGCACAGTTTGCGATCGCGCGCTTTTTCAGCCTGGTAGGTTTACTTTGGCGAGt                                                                 <  2:134852/81‑1 (MQ=255)
              gaATCGCTTCTTCCAGGTTGGCGCACAGTTTGCGATCGCGCGCTTTTTCAGCCTGGTAGGTTTACTTTGGCGAGTTCTgg                                                            >  2:134898/1‑80 (MQ=255)
              gaATCGCTTCTTCCAGGTTGGCGCACAGTTTGCGATCGCGCGCTTTTTCAGCCTGGTAGGTTTACTTTGGCGAGTTCTgg                                                            >  2:134900/1‑80 (MQ=255)
               aaTCGCTTCTTCCAGGTTGGCGCACAGTTTGCGATCGCGCGCTTTTTCAGCCTGGTAGGTTTACTTTGGCGAGTTCTGGa                                                           >  1:134911/1‑80 (MQ=255)
                attgCTTCTTCCAGGTTGGCGCACAGTTTGCGATCGCGCGCTTTTTCAGCCTGGTAGGTTTACTTTGGCGAGTTCTGGa                                                           >  1:134904/4‑79 (MQ=255)
                aTCGCTTCTTCCAGGTTGGCGCACAGTTTGCGATCGCGCGCTTTTTCAGCCTGGTAGGTTTACTTTGGCGAGTTCTGGaaa                                                         <  2:134853/81‑1 (MQ=255)
                   gCTTCTTCCAGGTTGGCGCACAGTTTGCGATCGCGCGCTTTTTCAGCCTGGTAGGTTTACTTTGGCGAGTTCTGGAAAGc                                                       <  2:134854/80‑1 (MQ=255)
                       cttcCAGGTTGGCGCACAGTTTGCGATCGCGCGCTTTTTCAGCCTGGTAGGTTTACTTTGGCGAGTTCTGGAAAGCGGc                                                    >  2:134913/1‑79 (MQ=255)
                       cttcCAGGTTGGCGCACAGTTTGCGATCGCGCGCTTTTTCAGCCTGGTAGGTTTACTTTGGCGAGTTCTGGAAAGCGGCa                                                   <  1:134855/80‑1 (MQ=255)
                         tcCAGGTTGGCGCACAGTTTGCGATCGCGCGCTTTTTCAGCCTGGTAGGTTTACTTTGGCGAGTTCTGGAAAGCGGc                                                    >  1:134905/1‑77 (MQ=255)
                            aGGTTGGCGCACAGTTTGCGATCGCGCGCTTTTTCAGCCTGGTAGGTTTACTTTGGCGAGTTCTGGAAAGCGGCAAGATCg                                             >  2:134902/1‑81 (MQ=255)
                              gTTGGCGCACAGTTTGCGATCGCGCGCTTTTTCAGCCTGGTAGGTTTACTTTGGCGAGTTCTGGAAAGCGGCAAGATCGCa                                           <  1:134856/81‑1 (MQ=255)
                                 ggCGCACAGTTTGCGATCGCGCGCTTTTTCAGCCTGGTAGGTTTACTTTGGCGAGTTCTGGAAAGCGGCAAGATCGCAtt                                         >  2:134901/1‑80 (MQ=255)
                                  gcgcACAGTTTGCGATCGCGCGCTTTTTCAGCCTGGTAGGTTTACTTTGGCGAGTTCTGGAAAGCGGCAAGATCGCATTCa                                       <  2:134857/81‑1 (MQ=255)
                                      acaGTTTGCGATCGCGCGCTTTTTCAGCCTGGTAGGTTTACTTTGGCGAGTTCTGGAAAGCGGCAAGATCGCATTCAgcgc                                   <  1:134859/81‑1 (MQ=255)
                                      acaGTTTGCGATCGCGCGCTTTTTCAGCCTGGTAGGTTTACTTTGGCGAGTTCTGGAAAGCGGCAAGATCGCATTCAgcgc                                   <  1:134858/81‑1 (MQ=255)
                                         gTTTGCGATCGCGCGCTTTTTCAGCCTGGTAGGTTTACTTTGGCGAGTTCTGGAAAGCGGCAAGATCGCATTCAGCGcgac                                <  2:134860/81‑1 (MQ=255)
                                              cGATCGCGCGCTTTTTCAGCCTGGTAGGTTTACTTTGGCGAGTTCTGGAAAGCGGCAAGATCGCATTCAGCGCGACGAtt                            <  2:134861/80‑1 (MQ=255)
                                               gATCGCGCGCTTTTTCAGCCTGGTAGGTTTACTTTGGCGAGTTCTGGAAAGCGGCAAGATCGCATTCAGCGCGACGATTcc                          <  2:134862/81‑1 (MQ=255)
                                               gATCGCGCGCTTTTTCAGCCTGGTAGGTTTACTTTGGCGAGTTCTGGAAAGCGGCAAGATCGCATTCAGCGCGACGATTcc                          <  1:134863/81‑1 (MQ=255)
                                                  cgcgcgCTTTTTCAGCCTGGTAGGTTTACTTTGGCGAGTTCTGGAAAGCGGCAAGATCGCATTCAGCGCGACGATTCCAgg                       >  1:134926/1‑81 (MQ=255)
                                                   gcgcgcTTTTTCAGCCTGGTAGGTTTACTTTGGCGAGTTCTGGAAAGCGGCAAGATCGCATTCAGCGCGACGATTCCAgg                       <  1:134864/80‑1 (MQ=255)
                                                   gcgcgcTTTTTCAGCCTGGTAGGTTTACTTTGGCGAGTTCTGGAAAGCGGCAAGATCGCATTCAGCGCGACGATTCCAGGc                      >  1:134921/1‑81 (MQ=255)
                                                     gcgcTTTTTCAGCCTGGTAGGTTTACTTTGGCGAGTTCTGGAAAGCGGCAAGATCGCATTCAGCGCGACGATTCCAGGCCg                    <  1:134865/81‑1 (MQ=255)
                                                       gcTTTTTCAGCCTGGTAGGTTTACTTTGGCGAGTTCTGGAAAGCGGCAAGATCGCATTCAGCGCGACGATTCCAGGCCGCt                  <  2:134867/81‑1 (MQ=255)
                                                       gcTTTTTCAGCCTGGTAGGTTTACTTTGGCGAGTTCTGGAAAGCGGCAAGATCGCATTCAGCGCGACGATTCCAGGCCGCt                  <  1:134866/81‑1 (MQ=255)
                                                         tttttCAGCCTGGTAGGTTTACTTTGGCGAGTTCTGGAAAGCGGCAAGATCGCATTCAGCGCGACGATTCCATGCCGCTAc                >  1:134915/1‑81 (MQ=255)
                                                         tttttCAGCCTGGTAGGTTTACTTTGGCGAGTTCTGGAAAGCGGCAAGATCGCATTCAGCGCGACGATTCCAGGCCGCTAc                >  2:134920/1‑81 (MQ=255)
                                                              cAGCCTGGTAGGTTTACTTTGGCGAGTTCTGGAAAGCGGCAAGATCGCATTCAGCGCGACGATTCCAGGCCGCTACCCGt            <  2:134869/80‑1 (MQ=255)
                                                              cAGCCTGGTAGGTTTACTTTGGCGAGTTCTGGAAAGCGGCAAGATCGCATTCAGCGCGACGATTCCAGGCCGCTACCCGt            <  1:134868/80‑1 (MQ=255)
                                                                gCCTGGTAGGTTTACTTTGGCGAGTTCTGGAAAGCGGCAAGATCGCATTCAGCGCGACGATTCCAGGCCGCTACCCGTTCt         <  1:134870/81‑1 (MQ=255)
                                                                 ccTGGTAGGTTTACTTTGGCGAGTTCTGGAAAGCAGCAAGATCGCATTCAGCGCGACGATTCCAGGCCGCTACCCGTTCtt        <  1:134871/81‑1 (MQ=255)
                                                                    ggTAGGTTTACTTTGGCGAGTTCTGGAAAGCGGCAAGATCGCATTCAGCGCGACGATTCCAGGCCGCTACCCGTTCTTGTc     >  1:134908/1‑81 (MQ=255)
                                                                      tAGGTTTACTTTGGCGAGTTCTGGAAAGCGGCAAGATCGCATTCAGCGCGACGATTCCAGGCCGCTACCCGTTCTTGTCGt   >  2:134907/1‑81 (MQ=255)
                                                                       aGGTTTACTTTGGCGAGTTCTGGAAAGCGGCAAGATCGCATTCAGCGCGACGATTCCAGGCCGCTACCCGTTCTTGTCGtt  >  1:134903/1‑81 (MQ=255)
                                                                             |                                                                          
TAAACCAGAGCGACGAATCGCTTCTTCCAGGTTGGCGCACAGTTTGCGATCGCGCGCTTTTTCAGCCTGGTAGGTTTGCTTTGGCGAGTTCTGGAAAGCGGCAAGATCGCATTCAGCGCGACGATTCCAGGCCGCTACCCGTTCTTGTCGTT  >  NC_000913/649395‑649546

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑