Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	94,873	T→A	L75Q (CTG→CAG)	murF →	D‑alanyl‑D‑alanine‑adding enzyme

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	94,873	0	T	A	100.0%	146.2 / NA	40	L75Q (CTG→CAG)	murF	D‑alanyl‑D‑alanine‑adding enzyme
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (23/17);  total (23/17)

TGCCCACGATTTTGCCGACCAGGCGAAAGCTGGCGGCGCAGGCGCACTACTGGTTAGCCGTCCGCTGGACATCGACCTGCCGCAGTTAATCGTCAAGGATACGCGTCTGGCGTTTGGTGAACTGGCTGCATGGGTTCGCCAGCAAGTTCCGGCGC  >  NC_000913/94796‑94950                                                                              |                                                                              tGCCCACGATTTTGCCGACCAGGCGAAAGCTGGCGGCGCAGGCGCACTACTGGTTAGCCGTCCGCTGGACATCGACCAGcc                                                                            <  2:21362/81‑1 (MQ=255)  gCCCACGATTTTGCCGACCAGGCGAAAGCTGGCGGCGCAGGCGCACTACTGGTTAGCCGTCCGCTGGACATCGACCAGCCg                                                                           >  2:21430/1‑81 (MQ=255)  gCCCACGATTTTGCCGACCAGGCGAAAGCTGGCGGCGCAGGCGCACTACTGGTTAGCCGTCCGCTGGACATCGACCAGCCg                                                                           >  2:21423/1‑81 (MQ=255)   cccACGATTTTGCCGACCAGGCGAAAGCTGGCGGCGCAGGCGCACTACTGGTTAGCCGTCCGCTGGACATCGACCAGCCGc                                                                          <  2:21363/81‑1 (MQ=255)    ccACGATTTTGCCGACCAGGCGAAAGCTGGCGGCGCAGGCGCACTACTGGTTAGCCGTCCGCTGGACATCGACCAGCCg                                                                           >  1:21402/1‑79 (MQ=255)              gCCGACCAGGCGAAAGCTGGCGGCGCAGGCGCACTACTGGTTAGCCGTCCGCTGGACATCGACCAGCCGCAGTTAATCGt                                                                >  2:21436/1‑80 (MQ=255)              gCCGACCAGGCGAAAGCTGGCGGCGCAGGCGCACTACTGGTTAGCCGTCCGCTGGACATCGACCAGCCGCAGTTAATCGt                                                                >  2:21429/1‑80 (MQ=255)                 gACCAGGCGAAAGCTGGCGGCGCAGGCGCACTACTGGTTAGCCGTCCGCTGGACATCGACCAGCCGCAGTTAATCGTCAAg                                                            >  1:21422/1‑81 (MQ=255)                       gCGAAAGCTGGCGGCGCAGGCGCACTACTGGTTAGCCGTCCGCTGGACATCGACCAGCCGCAGTTAATCGTCAAGGATAcg                                                      >  2:21417/1‑81 (MQ=255)                       gCGAAAGCTGGCGGCGCAGGCGCACTACTGGTTAGCCGTCCGCTGGACATCGACCAGCCGCAGTTAATCGTCAAGGATAcg                                                      >  1:21416/1‑81 (MQ=255)                        cGAAAGCTGGCGGCGCAGGCGCACTACTGGTTAGCCGTCCGCTGGACATCGACCAGCCGCAGTTAATCGTCAAGGATAcgc                                                     <  1:21365/81‑1 (MQ=255)                        cGAAAGCTGGCGGCGCAGGCGCACTACTGGTTAGCCGTCCGCTGGACATCGACCAGCCGCAGTTAATCGTCAAGGATAcgc                                                     <  2:21364/81‑1 (MQ=255)                         gAAAGCTGGCGGCGCAGGCGCACTACTGGTTAGCCGTCCGCTGGACATCGACCAGCCGCAGTTAATCGTCAAGGATAcgcg                                                    >  1:21409/1‑81 (MQ=255)                         gAAAGCTGGCGGCGCAGGCGCACTACTGGTTAGCCGTCCGCTGGACATCGACCAGCCGCAGTTAATCGTCAAGGATAcgcg                                                    <  2:21366/81‑1 (MQ=255)                          aaaGCTGGCGGCGCAGGCGCACTACTGGTTAGCCGTCCGCTGGACATCGACCAGCCGCAGTTAATCGTCAAGGATACGCGt                                                   >  1:21420/1‑81 (MQ=255)                            aGCTGGCGGCGCAGGCGCACTACTGGTTAGCCGTCCGCTGGACATCGACCAGCCGCAGTTAATCGTCAAGGATACGCGTCt                                                 <  1:21367/81‑1 (MQ=255)                                 gcggcgCAGGCGAACTACTGGTTAGCCGTCCGCTGGACATCGACCAGCCGCAGTTAATCGTCAAGGATACGCGTCTGGCg                                             <  2:21368/80‑1 (MQ=255)                                   ggcgcaggcgcaCTACTGGTTAGCCGTCCGCTGGACATCGACCAGCCGCAGTTAATCGTCAAGGATACGCGTCTGGCGttt                                          >  1:21434/1‑81 (MQ=255)                                      gcaggcgcaCTACTGGTTAGCCGTCCGCTGGACATCGACCAGCCGCAGTTAATCGTCAAGGATACGCGTCTGGCGTTTGGt                                       <  1:21369/81‑1 (MQ=255)                                       caggcgcaCTACTGGTTAGCCGTCCGCTGGACATCGACCAGCCGCAGTTAATCGTCAAGGATACGCGTCTGGCGTTTGGt                                       >  2:21428/1‑80 (MQ=255)                                       caggcgcaCTACTGGTTAGCCGTCCGCTGGACATCGACCAGCCGCAGTTAATCGTCAAGGATACGCGTCTGGCGTTTGGTg                                      <  2:21370/81‑1 (MQ=255)                                         ggcgcaCTACTGGTTAGCCGTCCGCTGGACATCGACCAGCCGCAGTTAATCGTCAAGGATACGCGTCTGGCGTTTGGTGaa                                    >  2:21425/1‑81 (MQ=255)                                           cgcaCTACTGGTTAGCCGTCCGCTGGACATCGACCAGCCGCAGTTAATCGTCAAGGATACGCGTCTGGCGTTTGGTGAACt                                  <  1:21371/81‑1 (MQ=255)                                              actactGGTTAGCCGTCCGCTGGACATCGACCAGCCGCAGTTAATCGTCAAGGATACGCGTCTGGCGTTTGGTGAACTGGc                               >  2:21445/1‑81 (MQ=255)                                              actactGGTTAGCCGTCCGCTGGACATCGACCAGCCGCAGTTAATCGTCAAGGATACGCGTCTGGCGTTTGGTGAACTGGc                               <  1:21372/81‑1 (MQ=255)                                              actactGGTTAGCCGTCCGCTGGACATCGACCAGCCGCAGTTAATCGTCAAGGATACGCGTCTGGCGTTTGGTGAACTGGc                               >  2:21426/1‑81 (MQ=255)                                              actactGGTTAGCCGTCCGCTGGACATCGACCAGCCGCAGTTAATCGTCAAGGATACGCGTCTGGCGTTTGGTGAACTGGc                               >  1:21419/1‑81 (MQ=255)                                               ctactGGTTAGCCGTCCGCTGGACATCGACCAGCCGCAGTTAATCGTCAAGGATACGCGTCTGGCGTTTGGTGAACTGGCt                              >  2:21413/1‑81 (MQ=255)                                               ctactGGTTAGCCGTCCGCTGGACATCGACCAGCCGCAGTTAATCGTCAAGGATACGCGTCTGGCGTTTGGTGAACTGGCt                              <  2:21373/81‑1 (MQ=255)                                               ctactGGTTAGCCGTCCGCTGGACATCGACCAGCCGCAGTTAATCGTCAAGGATACGCGTCTGGCGTTTGGTGAACTGGCt                              >  1:21424/1‑81 (MQ=255)                                               ctactGGTTAGCCGTCCGCTGGACATCGACCAGCCGCAGTTAATCGTCAAGGATACGCGTCTGGCGTTTGGTGAACTGGCt                              >  2:21440/1‑81 (MQ=255)                                                        aGCCGTCCGCTGGACATCGACCAGCCGCAGTTAATCGTCAAGGATACGCGTCTGGCGTTTGGTGAACTGGCTGCATGGGtt                     >  1:21448/1‑81 (MQ=255)                                                            gTCCGCTGGACATCGACCAGCCGCAGTTAATCGTCAAGGATACGCGTCTGGCGTTTGGTGAACTGGCTGCATGGGTTCGcc                 <  1:21374/81‑1 (MQ=255)                                                             tCCGCTGGACATCGACCAGCCGCAGTTAATCGTCAAGGATACGCGTCTGGCGTTTGGTGAACTGGCTGCATGGGTTCGCCa                <  1:21376/81‑1 (MQ=255)                                                             tCCGCTGGACATCGACCAGCCGCAGTTAATCGTCAAGGATACGCGTCTGGCGTTTGGTGAACTGGCTGCATGGGTTCGCCa                <  2:21375/81‑1 (MQ=255)                                                                 cTGGACATCGACCAGCCGCAGTTAATCGTCAAGGATACGCGTCTGGCGTTTGGTGAACTGGCTGCATGGGTTCGCCAGc              >  2:21431/1‑79 (MQ=255)                                                                   ggACATCGACCAGCCGCAGTTAATCGTCAAGGATACGCGTCTGGCGTTTGGTGAACTGGCTGCATGGGTTCGCCAGCAAGt          >  2:21437/1‑81 (MQ=255)                                                                      cATCGACCAGCCGCAGTTAATCGTCAAGGATACGCGTCTGGCGTTTGGTGAACTGGCTGCATGGGTTCGCCAGCAAGTTcc       <  2:21377/81‑1 (MQ=255)                                                                          gACCAGCCGCAGTTAATCGTCAAGGATACGCGTCTGGCGTTTGGTGAACTGGCTGCATGGGTTCGCCAGCAAGTTCCGgcg   <  2:21378/81‑1 (MQ=255)                                                                           aCCAGCCGCAGTTAATCGTCAAGGATACGCGTCTGGCGTTTGGTGAACTGGCTGCATGGGTTCGCCAGCAAGTTCCGgcgc  >  2:21442/1‑81 (MQ=255)                                                                              |                                                                              TGCCCACGATTTTGCCGACCAGGCGAAAGCTGGCGGCGCAGGCGCACTACTGGTTAGCCGTCCGCTGGACATCGACCTGCCGCAGTTAATCGTCAAGGATACGCGTCTGGCGTTTGGTGAACTGGCTGCATGGGTTCGCCAGCAAGTTCCGGCGC  >  NC_000913/94796‑94950

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑