Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	693,279	G→A	R34C (CGC→TGC)	ybeZ ←	PhoH‑like protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	693,279	0	G	A	100.0%	103.2 / NA	29	R34C (CGC→TGC)	ybeZ	PhoH‑like protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (9/20);  total (9/20)

CGCAGCGGTGACGCAAATCGGACGGCCGGTCAGTTTAAAGTGGTTATCGCGGCGATTGATCTCGATGCCGAGACGGCGTTCGAGCTGCTTGATGTTGTCATCAAACGGGCCGCACAGGCTCAACAGACGCGCATTGTCTGCTGGCTCCAGGGT  >  NC_000913/693202‑693354                                                                              |                                                                            cGCAGCGGTGACGCAAATCGGACGGCCGGTCAGTTTAAAGTGGTTATCGCGGCGATTGATCTCGATGCCGAGACGGCATTc                                                                          >  2:143213/1‑81 (MQ=255)  gCAGCGGTGACGCAAATCGGACGGCCGGTCAGTTTAAAGTGGTTATCGCGGCGATTGATCTCGATGCCGAGACGGCATTCg                                                                         <  2:143171/81‑1 (MQ=255)   cAGCGGTGACGCAAATCGGACGGCCGGTCAGTTTAAAGTGGTTATCGCGGCGATTGATCTCGATGCCGAGACGGCATTc                                                                          >  2:143222/1‑79 (MQ=255)    aGCGGTGACGCAAATCGGACGGCCGGTCAGTTTAAAGTGGTTATCGCGGCGATTGATCTCGATGCCGAGACGGCATTCGAg                                                                       <  1:143172/81‑1 (MQ=255)      cGGTGACGCAAATCGGACGGCCGGTCAGTTTAAAGTGGTTATCGCGGCGATTGATCTCGATGCCGAGACGGCATTCGAgct                                                                     >  1:143214/1‑81 (MQ=255)         tGACGCAAATCGGACGGCCGGTCAGTTTAAAGTGGTTATCGCGGCGATTGATCTCGATGCCGAGACGGCATTCGAgctgct                                                                  <  2:143173/81‑1 (MQ=255)         tGACGCAAATCGGACGGCCGGTCAGTTTAAAGTGGTTATCGCGGCGATTGATCTCGATGCCGAGACGGCATTCGAgctgct                                                                  <  2:143174/81‑1 (MQ=255)                aaTCGGACGGCCGGTCAGTTTAAAGTGGTTATCGCGGCGATTGATCTCGATGCCGAGACGGCATTCGAGCTGCTTGAtgtt                                                           >  2:143217/1‑81 (MQ=255)                       cGGCCGGTCAGTTTAAAGTGGTTATCGCGGCGATTGATCTCGATGCCGAGACGGCATTCGAGCTGCTTGATGTTGtcatca                                                    <  2:143175/81‑1 (MQ=255)                             gTCAGTTTAAAGTGGTTATCGCGGCGATTGATCTCGATGCCGAGACGGCATTCGAGCTGCTTGATGTTGTCATCAAACggg                                              >  1:143220/1‑81 (MQ=255)                                aGTTTAAAGTGGTTATCGCGGCGATTGATCTCGATGCCGAGACGGCATTCGAGCTGCTTGATGTTGTCATCAAACGGGcc                                            <  1:143176/80‑1 (MQ=255)                                        gTGGTTATCGCGGCGATTGATCTCGATGCCGAGACGGCATTCGAGCTGCTTGATGTTGTCATCAAACGGGCCGCACAGGCt                                   <  2:143177/81‑1 (MQ=255)                                           gTTATCGCGGCGATTGATCTCGATGCCGAGACGGCATTCGAGCTGCTTGATGTTGTCATCAAACGGGCCGCACAGGCTCaa                                <  2:143178/81‑1 (MQ=255)                                             tATCGCGGCGATTGATCTCGATGCCGAGACGGCATTCGAGCTGCTTGATGTTGTCATCAAACGGGCCGCACAGGCTCaa                                >  2:143223/1‑79 (MQ=255)                                                 gcggcgATTGATCTCGATGCCGAGACGGCATTCGAGCTGCTTGATGTTGTCATCAAACGGGCCGCACAGGCTCAACAGAc                           <  2:143179/80‑1 (MQ=255)                                                   ggcgATTGATCTCGATGCCGAGACGGCATTCGAGCTGCTTGATGTTGTCATCAAACGGGCCGCACAGGCTCAACAGAcgcg                        <  1:143180/81‑1 (MQ=255)                                                     cgATTGATCTCGATGCCGAGACGGCATTCGAGCTGCTTGATGTTGTCATCAAACGGGCCGCACAGGCTCAACAGACGCGCa                      <  2:143181/81‑1 (MQ=255)                                                      gATTGATCTCGATGCCGAGACGGCATTCGAGCTGCTTGATGTTGTCATCAAACGGGCCGCACAGGCTCAACAGACGCGCAt                     >  1:143221/1‑81 (MQ=255)                                                       aTTGATCTCGATGCCGAGACGGCATTCGAGCTGCTTGATGTTGTCATCAAACGGGCCGCACAGGCTCAACAGACGCGCAtt                    >  1:143232/1‑81 (MQ=255)                                                       aTTGATCTCGATGCCGAGACGGCATTCGAGCTGCTTGATGTTGTCATCAAACGGGCCGCACAGGCTCAACAGACGCGCAtt                    <  2:143182/81‑1 (MQ=255)                                                       aTTGATCTCGATGCCGAGACGGCATTCGAGCTGCTTGATGTTGTCATCAAACGGGCCGCACAGGCTCAACAGACGCGCAtt                    <  2:143183/81‑1 (MQ=255)                                                           aTCTCGATGCCGAGACGGCATTCGAGCTGCTTGATGTTGTCATCAAACGGGCCGCACAGGCTCAACAGACGCGCATTGTc                 <  2:143184/80‑1 (MQ=255)                                                             ctcGATGCCGAGACGGCATTCGAGCTGCTTGATGTTGTCATCAAACGGGCCGCACAGGCTCAACAGACGCGCATTGTctgc              >  2:143227/1‑81 (MQ=255)                                                                gATGCCGAGACGGCATTCGAGCTGCTTGATGTTGTCATCAAACGGGCCGCACAGGCTCAACAGACGCGCATTGTctgct             <  2:143185/79‑1 (MQ=255)                                                                  tGCCGAGACGGCATTCGAGCTGCTTGATGTTGTCATCAAACGGGCCGCACAGGCTCAACAGACGCGCATTGTCTGCTGGCt         <  2:143186/81‑1 (MQ=255)                                                                    ccGAGACGGCATTCGAGCTGCTTGATGTTGTCATCAAACGGGCCGCACAGGCTCAACAGACGCGCATTGTCTGCTGGCTcc       <  1:143188/81‑1 (MQ=255)                                                                    ccGAGACGGCATTCGAGCTGCTTGATGTTGTCATCAAACGGGCCGCACAGGCTCAACAGACGCGCATTGTCTGCTGGCTcc       <  1:143187/81‑1 (MQ=255)                                                                      gagaCGGCATTCGAGCTGCTTGATGTTGTCATCAAACGGGCCGCACAGGCTCAACAGACGCGCATTGTCTGCTGGCTCCAg     <  2:143189/81‑1 (MQ=255)                                                                         aCGGCATTCGAGCTGCTTGATGTTGTCATCAAACGGGCCGCACAGGCTCAACAGACGCGCATTGTCTGCTGGCTCCAGGGt  <  2:143190/81‑1 (MQ=255)                                                                              |                                                                            CGCAGCGGTGACGCAAATCGGACGGCCGGTCAGTTTAAAGTGGTTATCGCGGCGATTGATCTCGATGCCGAGACGGCGTTCGAGCTGCTTGATGTTGTCATCAAACGGGCCGCACAGGCTCAACAGACGCGCATTGTCTGCTGGCTCCAGGGT  >  NC_000913/693202‑693354

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑