Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	713,939	C→T	L128L (CTG→TTG)	pgm →	phosphoglucomutase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	713,939	0	C	T	100.0%	113.2 / NA	31	L128L (CTG→TTG)	pgm	phosphoglucomutase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (12/19);  total (12/19)

GCAGCGAACGGCGTTGATGTCATTGTGCAGGAAAACAATGGCTTCACCCCGACGCCTGCCGTTTCCAATGCCATCCTGGTTCACAATAAAAAAGGTGGCCCGCTGGCAGACGGTATCGTGATTACACCGTCCCATAACCCGCCGGAAGATGG  >  NC_000913/713864‑714015                                                                            |                                                                             gCAGCGAACGGCGTTGATGTCATTGTGCAGGAAAACAATGGCTTCACCCCGACGCCTGCCGTTTCCAATGCCATCTTGGtt                                                                         >  1:147198/1‑81 (MQ=255)  cAGCGAACGGCGTTGATGTCATTGTGCAGGAAAACAATGGCTTCACCCCGACGCCTGCCGTTTCCAATGCCATCTTGGtt                                                                         >  2:147195/1‑80 (MQ=255)        aCGGCGTTGATGTCATTGTGCAGGAAAACAATGGCTTCACCCCGACGCCTGCCGTTTCCAATGCCATCTTGGTTCACAATa                                                                  <  2:147150/81‑1 (MQ=255)           gCGTTGATGTCATTGTGCAGGAAAACAATGGCTTCACCCCGACGCCTGCCGTTTCCAATGCCATCTTGGTTCACAATaaa                                                                >  2:147199/1‑80 (MQ=255)           gCGTTGATGTCATTGTGCAGGAAAACAATGGCTTCACCCCGACGCCTGCCGTTTCCAATGCCATCTTGGTTCACAATaaa                                                                >  2:147187/1‑80 (MQ=255)               tGATGTCATTGTGCAGGAAAACAATGGCTTCACCCCGACGCCTGCCGTTTCCAATGCCATCTTGGTTCACAATAAAAAAgg                                                           >  1:147205/1‑81 (MQ=255)               tGATGTCATTGTGCAGGAAAACAATGGCTTCACCCCGACGCCTGCCGTTTCCAATGCCATCTTGGTTCACAATAAAAAAgg                                                           >  1:147193/1‑81 (MQ=255)                      aTTGTGCAGGAAAACAATGGCTTCACCCCGACGCCTGCCGTTTCCAATGCCATCTTGGTTCACAATAAAAAAGGTGGccc                                                     <  2:147153/80‑1 (MQ=255)                      aTTGTGCAGGAAAACAATGGCTTCACCCCGACGCCTGCCGTTTCCAATGCCATCTTGGTTCACAATAAAAAAGGTGGccc                                                     <  2:147151/80‑1 (MQ=255)                      aTTGTGCAGGAAAACAATGGCTTCACCCCGACGCCTGCCGTTTCCAATGCCATCTTGGTTCACAATAAAAAAGGTGGCCCg                                                    <  1:147152/81‑1 (MQ=255)                         gtgCAGGAAAACAATGGCTTCACCCCGACGCCTGCCGTTTCCAATGCCATCTTGGTTCACAATAAAAAAGGTGGCCCGCt                                                  <  1:147154/80‑1 (MQ=255)                         gtgCAGGAAAACAATGGCTTCACCCCGACGCCTGCCGTTTCCAATGCCATCTTGGTTCACAATAAAAAAGGTGGCCCGCt                                                  <  2:147155/80‑1 (MQ=255)                          tgCAGGAAAACAATGGCTTCACCCCGACGCCTGCCGTTTCCAATGCCATCTTGGTTCACAATAAAAAAGGTGGCCCGCTg                                                 <  2:147156/80‑1 (MQ=255)                            cAGGAAAACAATGGCTTCACCCCGACGCCTGCCGTTTCCAATGCCATCTTGGTTCACAATAAAAAAGGTGGCCCGCTGGc                                               <  1:147157/80‑1 (MQ=255)                                 aaaCAATGGCTTCACCCCGACGCCTGCCGTTTCCAATGCCATCTTGGTTCACAATAAAAAAGGTGGCCCGCTGGCAGACgg                                         >  2:147191/1‑81 (MQ=255)                                     aaTGGCTTCACCCCGACGCCTGCCGTTTCCAATGCCATCTTGGTTCACAATAAAAAAGGTGGCCCGCTGGCAGACGGTATc                                     <  1:147158/81‑1 (MQ=255)                                      aTGGCTTCACCCCGACGCCTGCCGTTTCCAATGCCATCTTGGTTCACAACAAAAAAGGTGGCCCGCTGGCAGACGGTATCg                                    >  2:147189/1‑81 (MQ=255)                                         gCTTCACCCCGACGCCTGCCGTTTCCAATGCCATCTTGGTTCACAATAAAAAAGGTGGCCCGCTGGCAGACGGTATCGTg                                  <  2:761683/80‑1 (MQ=255)                                             cACCCCGACGCCTGCCGTTTCCAATGCCATCTTGGTTCACAATAAAAAAGGTGGCCCGCTGGCAGACGGTATCGTGATTa                              <  1:147159/80‑1 (MQ=255)                                                 ccGACGCCTGCCGTTTCCAATGCCATCTTGGTTCACAATAAAAAAGGTGGCCCGCTGGCAGACGGTATCGTGATTACACCg                         >  1:147203/1‑81 (MQ=255)                                                    aCGCCTGCCGTTTCCAATGCCATCTTGGTTCACAATAAAAAAGGTGGCCCGCTGGCAGACGGTATCGTGATTACACCGTcc                      <  2:147160/81‑1 (MQ=255)                                                      gCCTGCCGTTTCCAATGCCATCTTGGTTCACAATAAAAAAGGTGGCCCGCTGGCAGACGGTATCGTGATTACACCGTCCCa                    >  1:147190/1‑81 (MQ=255)                                                       ccTGCCGTTTCCAATGCCATCTTGGTTCACAATAAAAAAGGTGGCCCGCTGGCAGACGGTATCGTGATTACACCGTCCCa                    >  2:147207/1‑80 (MQ=255)                                                           ccGTTTCCAATGCCATCTTGGTTCACAATAAAAAAGGTGGCCCGCTGGCAGACGGTATCGTGATTACACCGTCCCATaa                 <  1:147161/79‑1 (MQ=255)                                                             gTTTCCAATGCCATCTTGGTTCACAATAAAAAAGGTGGCCCGCTGGCAGACGGTATCGTGATTACACCGTCCCATAACccg             <  2:147162/81‑1 (MQ=255)                                                                 ccAATGCCATCTTGGTTCACAAT‑AAAAAGGTGGCCCGCTGGCAGACGGTATCGTGATTACACCGTCCCATAACCCGCCgg         <  1:147163/80‑1 (MQ=255)                                                                  cAATGCCATCTTGGTTCACAATAAAAAAGGTGGCCCGCTGGCAGACGGTATCGTGATTACACCGTCCCATAACCCGCCGGa        <  1:147164/81‑1 (MQ=255)                                                                    aTGCCATCTTGGTTCACAATAAAAAAGGTGGCCCGCTGGCAGACGGTATCGTGATTACACCGTCCCATAACCCGCCGGAAg      <  2:147165/81‑1 (MQ=255)                                                                       ccATCTTGGTTCACAATAAAAAAGGTGGCCCGCTGGCAGACGGTATCGTGATTACACCGTCCCATAACCCGCCGGAAGAt    <  2:147166/80‑1 (MQ=255)                                                                       ccATCTTGGTTCACAATAAAAAAGGTGGCCCGCTGGCAGACGGTATCGTGATTACACCGTCCCATAACCCGCCGGAAGAt    <  1:147167/80‑1 (MQ=255)                                                                        cATCTTGGTTCACAATAAAAAAGGTGGCCCGCTGGCAGACGGTATCGTGATTACACCGTCCCATAACCCGCCGGAAGAtgg  >  2:147209/1‑81 (MQ=255)                                                                            |                                                                             GCAGCGAACGGCGTTGATGTCATTGTGCAGGAAAACAATGGCTTCACCCCGACGCCTGCCGTTTCCAATGCCATCCTGGTTCACAATAAAAAAGGTGGCCCGCTGGCAGACGGTATCGTGATTACACCGTCCCATAACCCGCCGGAAGATGG  >  NC_000913/713864‑714015

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑