Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	714,976	G→A	L473L (CTG→CTA)	pgm →	phosphoglucomutase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	714,976	0	G	A	100.0%	123.3 / NA	34	L473L (CTG→CTA)	pgm	phosphoglucomutase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (11/23);  total (11/23)

GCAGGCAGCTGCGACTTCCGCACAAAAAGCGGCGCTGTCTAAGCTGTCTCCGGAAATGGTGAGCGCCAGCACCCTGGCAGGTGACCCGATCACCGCGCGCCTGACTGCTGCTCCGGGCAACGGTGCTTCTATTGGCGGTCTGAAAGT  >  NC_000913/714901‑715047                                                                            |                                                                        gcaggcagCTGCGACTTCCGCACAAAAAGCGGCGCTGTCTAAGCTGTCTCCGGAAATGGTGAGCGCCAGCACCCTAGCAg                                                                     <  1:147374/80‑1 (MQ=255)       agCTGCGACTTCCGCACAAAAAGCGGCGCTGTCTAAGCTGTCTCCGGAAATGGTGAGCGCCAGCACCCTAGCAGGTGAccc                                                              >  2:147421/1‑81 (MQ=255)       agCTGCGACTTCCGCACAAAAAGCGGCGCTGTCTAAGCTGTCTCCGGAAATGGTGAGCGCCAGCACCCTAGCAGGTGAccc                                                              <  2:147375/81‑1 (MQ=255)           gCGACTTCCGCACAAAAAGCGGCGCTGTCTAAGCTGTCTCTGGAAATGGTGAGCGCCAGCACCCTAGCAGGTGATCCGAt                                                           >  2:147416/1‑80 (MQ=255)            cGACTTCCGCACAAAAAGCGGCGCTGTCTAAGCTGTCTCCGGAAATGGTGAGCGCCAGCACCCTAGCAGGTGACCCGAt                                                           <  2:147376/79‑1 (MQ=255)            cGACTTCCGCACAAAAAGCGGCGCTGTCTAAGCTGTCTCCGGAAATGGTGAGCGCCAGCACCCTAGCAGGTGACCCGATc                                                          <  1:147377/80‑1 (MQ=255)             gACTTCCGCACAAAAAGCGGCGCTGTCTAAGCTGTCTCCGGAAATGGTGAGCGCCAGCACCCTAGCAGGTGACCCGATCAc                                                        >  1:147412/1‑81 (MQ=255)               cTTCCGCACAAAAAGCGGCGCTGTCTAAGCTGTCTCCGGAAATGGTGAGCGCCAGCACCCTAGCAGGTGACCCGATCACcg                                                      <  1:147378/81‑1 (MQ=255)                ttCCGCACAAAAAGCGGCGCTGTCTAAGCTGTCTCCGGAAATGGTGAGCGCCAGCACCCTAGCAGGTGACCCGATCACcgc                                                     >  1:147427/1‑81 (MQ=255)                  ccGCACAAAAAGCGGCGCTGTCTAAGCTGTCTCCGGAAATGGTGAGCGCCAGCACCCTAGCAGGTGACCCGATCACcgcg                                                    <  1:147379/80‑1 (MQ=255)                  ccGCACAAAAAGCGGCGCTGTCTAAGCTGTCTCCGGAAATGGTGAGCGCCAGCACCCTAGCAGGTGACCCGATCACcgc                                                     <  2:147380/79‑1 (MQ=255)                      acaAAAAGCGGCGCTGTCTAAGCTGTCTCCGGAAATGGTGAGCGCCAGCACCCTAGCAGGTGACCCGATCACCgcgcgcat                                               <  1:147381/81‑3 (MQ=255)                      acaAAAAGCGGCGCTGTCTAAGCTGTCTCCGGAAATGGTGAGCGCCAGCACCCTAGCAGGTGACCCGATCACCGCGCGCCt                                               <  1:147382/81‑1 (MQ=255)                      acaAAAAGCGGCGCTGTCTAAGCTGTCTCCGGAAATGGTGAGCGCCAGCACCCTAGCAGGTGACCCGATCACCGCGCGCCt                                               <  2:147383/81‑1 (MQ=255)                       caAAAAGCGGCGCTGTCTAAGCTGTCTCCGGAAATGGTGAGCGCCAGCACCCTAGCAGGTGACCCGATCACCGCGCGCCTg                                              <  2:147384/81‑1 (MQ=255)                         aaaaGCGGCGCTGTCTAAGCTGTCTCCGGAAATGGTGAGCGCCAGCACCCTAGCAGGTGACCCGATCACCGCGCGCCTGAc                                            >  1:147429/1‑81 (MQ=255)                          aaaGCGGCGCTGTCTAAGCTGTCTCCGGAAATGGTGAGCGCCAGCACCCTAGCAGGTGACCCGATCACCGCGCGCCTGAct                                           >  1:147414/1‑81 (MQ=255)                            aGCGGCGCTGTCTAAGCTGTCTCCGGAAATGGTGAGCGCCAGCACCCTAGCAGGTGACCCGATCACCGCGCGCCTGActgc                                         >  1:147424/1‑81 (MQ=255)                            aGCGGCGCTGTCTAAGCTGTCTCCGGAAATGGTGAGCGCCAGCACCCTAGCAGGTGACCCGATCACCGCGCGCCTGActgc                                         <  2:147385/81‑1 (MQ=255)                            aGCGGCGCTGTCTAAGCTGTCTCCGGAAATGGTGAGCGCCAGCACCCTAGCAGGTGACCCGATCACCGCGCGCCTGActgc                                         >  1:147419/1‑81 (MQ=255)                              cggcgCTGTCTAAGCTGTCTCCGGAAATGGTGAGCGCCAGCACCCTAGCAGGTGACCCGATCACCGCGCGCCTGActgctg                                       <  2:147386/81‑1 (MQ=255)                                  gcTGTCTAAGCTGTCTCCGGAAATGGTGAGCGCCAGCACCCTAGCAGGTGACCCGATCACCGCGCGCCTGACTGCTGCTcc                                   <  1:147387/81‑1 (MQ=255)                                  gcTGTCTAAGCTGTCTCCGGAAATGGTGAGCGCCAGCACCCTAGCAGGTGACCCGATCACCGCGCGCCTGACTGCTGCTcc                                   >  1:147420/1‑81 (MQ=255)                                      tCTAAGCTGTCTCCGGAAATGGTGAGCGCCAGCACCCTAGCAGGTGACCCGATCACCGCGCGCCTGACTGCTGCTCCgg                                 <  1:147388/79‑1 (MQ=255)                                          aGCTGTCTCCGGAAATGGTGAGCGCCAGCACCCTAGCAGGTGACCCGATCACCGCGCGCCTGACTGCTGCTCCGGGCAAc                            <  2:147389/80‑1 (MQ=255)                                          aGCTGTCTCCGGAAATGGTGAGCGCCAGCACCCTAGCAGGTGACCCGATCACCGCGCGCCTGACTGCTGCTCCGGGCAAc                            <  2:147390/80‑1 (MQ=255)                                            cTGTCTCCGGAAATGGTGAGCGCCAGCACCCTAGCAGGTGACCCGATCACCGCGCGCCTGACTGCTGCTCCGGGCAACGGt                         <  1:147391/81‑1 (MQ=255)                                                   cGGAAATGGTGAGCGCCAGCACCCTAGCAGGTGACCCGATCACCGCGCGCCTGACTGCTGCTCCGGGCAACGGTGCTTc                    <  2:147392/79‑1 (MQ=255)                                                         tGGTGAGCGCCAGCACCCTAGCAGGTGACCCGATCACCGCGCGCCTGACTGCTGCTCCGGGCAACGGTGCTTCTATTGGCg            <  1:147393/81‑1 (MQ=255)                                                           gTGAGCGCCAGCACCCTAGCAGGTGACCCGATCACCGCGCGCCTGACTGCTGCTCCGGGCAACGGTGCTTCTATTGGCGGt          <  1:147394/81‑1 (MQ=255)                                                           gTGAGCGCCAGCACCCTAGCAGGTGACCCGATCACCGCGCGCCTGACTGCTGCTCCGGGCAACGGTGCTTCTATTGGCGGt          <  2:147395/81‑1 (MQ=255)                                                              aGCGCCAGCACCCTAGCAGGTGACCCGATCACCGCGCGCCTGACTGCTGCTCCGGGCAACGGTGCTTCTATTGGCGGTCTg       <  2:147396/81‑1 (MQ=255)                                                                   cAGCACCCTAGCAGGTGACCCGATCACCGCGCGCCTGACTGCTGCTCCGGGCAACGGTGCTTCTATTGGCGGTCTGAAAGt  >  1:147426/1‑81 (MQ=255)                                                                    aGCACCCTAGCAGGTGACCCGATCACCGCGCGCCTGACTGCTGCTCCGGGCAACGGTGCTTCTATTGGCGGTCTGAAAGt  >  1:147425/1‑80 (MQ=255)                                                                            |                                                                        GCAGGCAGCTGCGACTTCCGCACAAAAAGCGGCGCTGTCTAAGCTGTCTCCGGAAATGGTGAGCGCCAGCACCCTGGCAGGTGACCCGATCACCGCGCGCCTGACTGCTGCTCCGGGCAACGGTGCTTCTATTGGCGGTCTGAAAGT  >  NC_000913/714901‑715047

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑