Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	742,374	C→T	T61T (ACG→ACA)	dtpD ←	dipeptide:H(+) symporter DtpD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	742,374	0	C	T	100.0%	95.2 / NA	27	T61T (ACG→ACA)	dtpD	dipeptide:H(+) symporter DtpD
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (17/10);  total (17/10)

CCATCAACAACGCCCCCAGCATCACCGCCATGCGATTGCCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCTAAATAACTCGTAGGCGTGAGTATCGTTGTATTTTAGTTGATTGGT  >  NC_000913/742298‑742448                                                                             |                                                                           ccATCAACAACGCCCCCAGCATCACCGCCATGCGATTGCCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGTGTGa                                                                        >  2:152663/1‑81 (MQ=255)  cATCAACAACGCCCCCAGCATCACCGCCATGCGATTGCCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGTGTGa                                                                        >  1:152661/1‑80 (MQ=255)   aTCAACAACGCCCCCAGCATCACCGCCATGCGATTGCCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGTGTGACa                                                                      >  1:152657/1‑81 (MQ=255)    tCAACAACGCCCCCAGCATCACCGCCATGCGATTGCCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGTGTGACa                                                                      >  2:152658/1‑80 (MQ=255)     caacaaCGCCCCCAGCATCACCGCCATGCGATTGCCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGTGTGACATa                                                                    >  2:152665/1‑81 (MQ=255)      aacaacGCCCCCAGCATCACCGCCATGCGATTGCCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGTGTGACATac                                                                   <  2:152613/81‑1 (MQ=255)            gCCCCCAGCATCACCGCCATGCGATTGCCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGTGTGACATACACCAGc                                                             <  2:152614/81‑1 (MQ=255)            gCCCCCAGCATCACCGCCATGCGATTGCCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGTGTGACATACACCAGc                                                             <  1:152615/81‑1 (MQ=255)                ccAGCATCACCGCCATGCGATTGCCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGTGTGACATACACCAGCGaa                                                          >  1:152646/1‑80 (MQ=255)                        aCCGCCATGCGATTGCCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGTGTGACATACACCAGCGAACAGTAGgc                                                  <  1:152616/80‑1 (MQ=255)                           gCCATGCGATTGCCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGTGTGACATACACCAGCGAACAGTAGGCGCTa                                              <  1:152617/81‑1 (MQ=255)                             cATGCGATTGCCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGTGTGACATACACCAGCGAACAGTAGGCGCTaaa                                            >  1:152659/1‑81 (MQ=255)                              aTGCGATTGCCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGTGTGACATACACCAGCGAACAGTAGGCGCTAAAt                                           <  2:152618/81‑1 (MQ=255)                                 cGATTGCCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGTGTGACATACACCAGCGAACAGTAGGCGCTAAATAAc                                        >  1:152664/1‑81 (MQ=255)                                   aTTGCCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGTGTGACATACACCAGCGAACAGTAGGCGCTAAATAACTc                                      >  1:152654/1‑81 (MQ=255)                                      gCCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGTGTGACATACACCAGCGAACAGTAGGCGCTAAATAACTCg                                     >  1:152653/1‑79 (MQ=255)                                      gCCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGTGTGACATACACCAGCGAACAGTAGGCGCTAAATAACTCGt                                    <  1:152619/80‑1 (MQ=255)                                         gagaACTTTATCCGCCAAAAAGCCACCGAGGATTGGTGTGACATACACCAGCGAACAGTAGGCGCTAAATAACTCGTAGGc                                >  1:152655/1‑81 (MQ=255)                                         gagaACTTTATCCGCCAAAAAGCCACCGAGGATTGGTGTGACATACACCAGCGAACAGTAGGCGCTAAATAACTCGTAGGc                                >  2:152670/1‑81 (MQ=255)                                           gaACTTTATCCGCCAAAAAGCCACCGAGGATTGGTGTGACATACACCAGCGAACAGTAGGCGCTAAATAACTCGTAGGCGt                              >  1:152660/1‑81 (MQ=255)                                           gaACTTTATCCGCCAAAAAGCCACCGAGGATTGGTGTGACATACACCAGCGAACAGTAGGCGCTAAATAACTCGTAGGCGt                              >  2:152669/1‑81 (MQ=255)                                              cTTTATCCGCCAAAAAGCCACCGAGGATTGGTGTGACATACACCAGCGAACAGTAGGCGCTAAATAACTCGTAGGCGTGAg                           >  2:152668/1‑81 (MQ=255)                                                  aTCCGCCAAAAAGCCACCGAGGATTGGTGTGACATACACCAGCGAACAGTAGGCGCTAAATAACTCGTAGGCGTGAGTAt                        <  2:152620/80‑1 (MQ=255)                                                  aTCCGCCAAAAAGCCACCGAGGATTGGTGTGACATACACCAGCGAACAGTAGGCGCTAAATAACTCGTAGGCGTGAGTAt                        <  2:152621/80‑1 (MQ=255)                                                         aaaaaGCCACCGAGGATTGGTGTGACATACACCAGCGAACAGTAGGCGCTAAATAACTCGTAGGCGTGAGTATCGTTGTAt                >  1:152667/1‑81 (MQ=255)                                                                     aGGATTGGTGTGACATACACCAGCGAACAGTAGGCGCTAAATAACTCGTAGGCGTGAGTATCGTTGTATTTTAGTTGATTg    <  2:152622/81‑1 (MQ=255)                                                                          tGGTGTGACATACACCAGCGAACAGTAGGCGCTAAATAACTCGTAGGCGTGAGTATCGTTGTATTTTAGTTGATTGGt  >  2:152673/1‑78 (MQ=255)                                                                             |                                                                           CCATCAACAACGCCCCCAGCATCACCGCCATGCGATTGCCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCTAAATAACTCGTAGGCGTGAGTATCGTTGTATTTTAGTTGATTGGT  >  NC_000913/742298‑742448

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑