Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	747,445	G→T	P109T (CCT→ACT)	abrB ←	putative regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	747,445	0	G	T	100.0%	111.0 / NA	31	P109T (CCT→ACT)	abrB	putative regulator
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (15/16);  total (15/16)

CGCCGTAATCTTGCGCCATAGCGACCATTGCCGCCGCGCCGCCAGGAGAGGAGCCCCAGGCACCGGTATTTCCAGGCAATGAGCTATAGCGCACCAATAACCAACCCACAATGGCGCTGGAAAGCAGCGTTACCAGTAAAATTGCAAGCACG  >  NC_000913/747370‑747521                                                                            |                                                                             cgcCGTAATCTTGCGCCATAGCGACCATTGCCGCCGCGCCGCCAGGAGAGGAGCCCCAGGCACCGGTATTTCCAGTCAAt                                                                          >  1:153690/1‑80 (MQ=255) cgcCGTAATCTTGCGCCATAGCGACCATTGCCGCCGCGCCGCCAGGAGAGGAGCCCCAGGCACCGGTATTTCCAGTCAAt                                                                          <  1:153647/80‑1 (MQ=255) cgcCGTAATCTTGCGCCATAGCGACCATTGCCGCCGCGCCGCCAGGAGAGGAGCCCCAGGCACCGGTATTTCCAGTCAATg                                                                         <  2:153648/81‑1 (MQ=255)     gTAATCTTGCGCCATAGCGACCATTGCCGCCGCGCCGCCAGGAGAGGAGCCCCAGGCACCGGTATTTCCAGTCAATGAGCt                                                                     <  2:153649/81‑1 (MQ=255)          cTTGCGCCATAGCGACCATTGCCGCCGCGCCGCCAGGAGAGGAGCCCCAGGCACCGGTATTTCCAGTCAATGAGCTATAgc                                                                >  2:153695/1‑81 (MQ=255)             gcgcCATAGCGACCATTGCCGCCGCGCCGCCAGGAGAGGAGCCCCAGGCACCGGTATTTCCAGTCAATGAGCTATAgcgc                                                              <  2:153651/80‑1 (MQ=255)             gcgcCATAGCGACCATTGCCGCCGCGCCGCCAGGAGAGGAGCCCCAGGCACCGGTATTTCCAGTCAATGAGCTATAgc                                                                <  2:153650/78‑1 (MQ=255)             gcgcCATAGCGACCATTGCCGCCGCGCCGCCAGGAGAGGAGCCCCAGGCACCGGTATTTCCAGTCAATGAGCTATAGCGCa                                                             >  2:153688/1‑81 (MQ=255)              cgcCATAGCGACCATTGCCGCCGCGCCGCCAGGAGAGGAGCCCCAGGCACCGGTATTTCCAGTCAATGAGCTATAGCGCa                                                             <  1:153652/80‑1 (MQ=255)              cgcCATAGCGACCATTGCCGCCGCGCCGCCAGGAGAGGAGCCCCAGGCACCGGTATTTCCAGTCAATGAGCTATAGCGCAc                                                            <  1:153653/81‑1 (MQ=255)                      cGACCATTGCCGCCGCGCCGCCAGGAGAGGAGCCCCAGGCACCGGTATTTCCAGTCAATGAGCTATAGCGCACCAATaacc                                                    <  1:153654/81‑1 (MQ=255)                        aCCATTGCCGCCGCGCCGCCAGGAGAGGAGCCCCAGGCACCGGTATTTCCAGTCAATGAGCTATAGCGCACCAATaaccaa                                                  >  1:153687/1‑81 (MQ=255)                                     cgccgcCAGGAGAGGAGCCCCAGGCACCGGTATTTCCAGTCAATGAGCTATAGCGCACCAATAACCAACCCACAATGgcgc                                     >  1:153694/1‑81 (MQ=255)                                       ccgccAGGAGAGGAGCCCCAGGCACCGGTATTTCCAGTCAATGAGCTATAGCGCACCAATAACCAACCCACAATGGCGCTg                                   <  1:153655/81‑1 (MQ=255)                                       ccgccAGGAGAGGAGCCCCAGGCACCGGTATTTCCAGTCAATGAGCTATAGCGCACCAATAACCAACCCACAATGGCGCTg                                   >  1:153705/1‑81 (MQ=255)                                        cgccAGGAGAGGAGCCCCAGGCACCGGTATTTCCAGTCAATGAGCTATAGCGCACCAATAACCAACCCACAATGGCGCTgg                                  <  2:153656/81‑1 (MQ=255)                                            aggagaggagCCCCAGGCACCGGTATTTCCAGTCAATGAGCTATAGCGCACCAATAACCAACCCACAATGGCGCTGGAAag                              >  1:153711/1‑81 (MQ=255)                                                gaggagCCCCAGGCACCGGTATTTCCAGTCAATGAGCTATAGCGCACCAATAACCAACCCACAATGGCGCTGGAAagcagc                          >  2:153697/1‑81 (MQ=255)                                                  ggagCCCCAGGCACCGGTATTTCCAGTCAATGAGCTATAGCGCACCAATAACCAACCCACAATGGCGCTGGAAAGCAGCGt                        >  1:806754/1‑81 (MQ=255)                                                    agCCCCAGGCACCGGTATTTCCAGTCAATGAGCTATAGCGCACCAATAACCAACCCACAATGGCGCTGGAAAGCAGCGTTa                      >  1:153699/1‑81 (MQ=255)                                                       cccAGGCACCGGTATTTCCAGTCAATGAGCTATAGCGCACCAATAACCAACCCACAATGGCGCTGGAAAGCAGCGTTAc                     >  2:153725/1‑79 (MQ=255)                                                               ccGGTATTTCCAGTCAATGAGCTATAGCGCACCAATAACCAACCCACAATGGCGCTGGAAAGCAGCGTTACCAGTAAAAtt           <  2:153657/81‑1 (MQ=255)                                                                 ggTATTTCCAGTCAATGAGCTATAGCGCACCAATAACCAACCCACAATGGCGCTGGAAAGCAGCGTTACCAGTAAAATTg          >  2:153708/1‑80 (MQ=255)                                                                  gTATTTCCAGTCAATGAGCTATAGCGCACCAATAACCAACCCACAATGGCGCTGGAAAGCAGCGTTACCAGTAAAATTGCa        <  1:153658/81‑1 (MQ=255)                                                                     tttCCAGTCAATGAGCTATAGCGCACCAATATCCAACCCACAATGGCGCTGGAAAGCAGCGTTACCAGTAAAATTGCAAg      >  2:153716/1‑80 (MQ=255)                                                                     tttCCAGTCAATGAGCTATAGCGCACCAATAACCAACCCACAATGGCGCTGGAAAGCAGCGTTACCAGTAAAATTGCAAg      <  1:153659/80‑1 (MQ=255)                                                                      ttCCAGTCAATGAGCTATAGCGCACCAATAACCAACCCACAATGGCGCTGGAAAGCAGCGTTACCAGTAAAATTGCAAGCa    >  1:153707/1‑81 (MQ=255)                                                                       tCCAGTCAATGAGCTATAGCGCACCAATAACCAACCCACAATGGCGCTGGAAAGCAGCGTTACCAGTAAAATTGCAAGCAc   >  1:153703/1‑81 (MQ=255)                                                                        ccAGTCAATGAGCTATAGCGCACCAATAACCAACCCACAATGGCGCTGGAAAGCAGCGTTACCAGTAAAATTGCAAGCAc   <  2:153660/80‑1 (MQ=255)                                                                         cAGTCAATGAGCTATAGCGGACCAATAACCAACCCACAATGGCGCTGGAAAGCAGCGTTACCAGTAAAATTGCAAGCACg  <  1:153661/80‑1 (MQ=255)                                                                         cAGTCAATGAGCTATAGCGCACCAATAACCAACCCACAATGGCGCTGGAAAGCAGCGTTACCAGTAAAATTGCAAGCACg  <  1:153662/80‑1 (MQ=255)                                                                            |                                                                             CGCCGTAATCTTGCGCCATAGCGACCATTGCCGCCGCGCCGCCAGGAGAGGAGCCCCAGGCACCGGTATTTCCAGGCAATGAGCTATAGCGCACCAATAACCAACCCACAATGGCGCTGGAAAGCAGCGTTACCAGTAAAATTGCAAGCACG  >  NC_000913/747370‑747521

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑