Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	808,820	G→T	G146G (GGC→GGA)	bioA ←	adenosylmethionine‑8‑amino‑7‑oxononanoate aminotransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	808,820	0	G	T	100.0%	100.3 / NA	28	G146G (GGC→GGA)	bioA	adenosylmethionine‑8‑amino‑7‑oxononanoate aminotransferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (13/15);  total (13/15)

TTTTCTGGCAGGTAGCCTTTCCACAGACTGTGCATTGAGTTATCCGGATCGCACACCGACATCGCGCCAAAGGTATCGCCATGATAACCATTGCGGAAGGTCAGAAAACGCTGGCGCGCTTCGCCTTTGGCTTGCCAGTACTGCAACGCCA  >  NC_000913/808743‑808893                                                                              |                                                                          ttttCTGGCAGGTAGCCTTTCCACAGACTGTGCATTGAGTTATCCGGATCGCACGCCGACATCGCGCCAAAGGTATCTCCa                                                                        <  2:165272/81‑1 (MQ=255)   ttCTGGCAGGTAGCCTTTCCACAGACTGTGCATTGAGTTATCCGGATCGCACACCGACATCGCGCCAAAGGTATCTCCAt                                                                       <  2:165273/80‑1 (MQ=255)    tCTGGCAGGTAGCCTTTCCACAGACTGTGCATTGAGTTATCCGGATCGCACACCGACATCGCGCCAAAGGTATCTCCATg                                                                      <  2:165275/80‑1 (MQ=255)    tCTGGCAGGTAGCCTTTCCACAGACTGTGCATTGAGTTATCCGGATCGCACACCGACATCGCGCCAAAGGTATCTCCATg                                                                      <  2:165274/80‑1 (MQ=255)       ggCAGGTAGCCTTTCCACAGACTGTGCATTGAGTTATCCGGATCGCACACCGACATCGCGCCAAAGGTATCTCCATGATaa                                                                  >  1:165305/1‑81 (MQ=255)        gCAGGTAGCCTTTCCACAGACTGTGCATTGAGTTATCCGGATCGCACACCGACATCGCGCCAAAGGTATCTCCATGATaa                                                                  <  2:165276/80‑1 (MQ=255)            gTAGCCTTTCCACAGACTGTGCATTGAGTTATCCGGATCGCACACCGACATCGCGCCAAAGGTATCTCCATGATAACCAtt                                                             <  2:165277/81‑1 (MQ=255)              aGCCTTTCCACAGACTGTGCATTGAGTTATCCGGATCGCACACCGACATCGCGCCAAAGGTATCTCCATGATAACCATTGc                                                           >  2:165315/1‑81 (MQ=255)              aGCCTTTCCACAGACTGTGCATTGAGTTATCCGGATCGCACACCGACATCGCGCCAAAGGTATCTCCATGATAACCATTGc                                                           >  2:165312/1‑81 (MQ=255)                  tttCCACAGACTGTGCATTGAGTTATCCGGATCGCACACCGACATCGCGCCAAAGGTATCTCCATGATAACCATTGCgg                                                         >  2:165311/1‑79 (MQ=255)                    tCCACAGACTGTGCATTGAGTTATCCGGATCGCACACCGACATCGCGCCAAAGGTATCTCCATGATAACCATTGCGGaa                                                       <  2:165278/79‑1 (MQ=255)                      cacaGACTGTGCATTGAGTTATCCGGATCGCACACCGACATCGCGCCAAAGGTATCTCCATGATAACCATTGCGGAAGGTc                                                   <  2:165279/81‑1 (MQ=255)                        caGACTGTGCATTGAGTTATCCGGATCGCACACCGACATCGCGCCAAAGGTATCTCCATGATAACCATTGCGGAAGGt                                                    >  1:165322/1‑78 (MQ=255)                         aGACTGTGCATTGAGTTATCCGGATCGCACACCGACATCGCGCCAAAGGTATCTCCATGATAACCATTGCGGAAGGTCAg                                                 >  1:165321/1‑80 (MQ=255)                           aCTGTGCATTGAGTTATCCGGATCGCACACCGACATCGCGCCAAAGGTATCTCCATGATAACCATTGCGGAAGGTCAGaa                                               >  2:165318/1‑80 (MQ=255)                               tgCATTGAGTTATCCGGATCGCACACCGACATCGCGCCAAAGGTATCTCCATGATAACCATTGCGGAAGGTCAGAAAACGc                                          <  2:165280/81‑1 (MQ=255)                                   ttGAGTTATCCGGATCGCACACCGACATCGCGCCAAAGGTATCTCCATGATAACCATTGCGGAAGGTCAGAAAACGCTGgc                                      <  1:165281/81‑1 (MQ=255)                                          aTCCGGATCGCACACCGACATCGCGCCAAAGGTATCTCCATGATAACCATTGCGGAAGGTCAGAAAACGCTGGCGCGCTTc                               >  2:165313/1‑81 (MQ=255)                                           tCCGGATCGCACACCGACATCGCGCCAAAGGTATCTCCATGATAACCATTGCGGAAGGTCAGAAAACGCTGGCGCGCTTc                               <  1:165282/80‑1 (MQ=255)                                            ccGGATCGCACACCGACATCGCGCCAAAGGTATCTCCATGATAACCATTGCGGAAGGTCAGAAAACGCTGGCGCGCTTCGc                             >  2:165323/1‑81 (MQ=255)                                             cGGATCGCACACCGACATCGCGCCAAAGGTATCTCCATGATAACCATTGCGGAAGGTCAGAAAACGCTGGCGCGCTTCGcc                            <  2:165283/81‑1 (MQ=255)                                              ggATCGCACACCGACATCGCGCCAAAGGTATCTCCATGATAACCATTGCGGAAGGTCAGAAAACGCTGGCGCGCTTCGCCt                           <  2:165284/81‑1 (MQ=255)                                                  cGCACACCGACATCGCGCCAAAGGTATCTCCATGATAACCATTGCGGAAGGTCAGAAAACGCTGGCGCGCTTCGCCTTTgg                       >  1:165314/1‑81 (MQ=255)                                                       acCGACATCGCGCCAAAGGTATCTCCATGATAACCATTGCGGAAGGTCAGAAAACGCTGGCGCGCTTCGCCTTTGGCTTGc                  <  1:165285/81‑1 (MQ=255)                                                        ccGACATCGCGCCAAAGGTATCTCCATGATAACCATTGCGGAAGGTCAGAAAACGCTGGCGCGCTTCGCCTTTGGCTTGc                  >  2:165310/1‑80 (MQ=255)                                                         cGACATCGCGCCAAAGGTATCTCCATGATAACCATTGCGGAAGGTCAGAAAACGCTGGCGCGCTTCGCCTTTGGCTTGCCa                <  1:165286/81‑1 (MQ=255)                                                                  gcCAAAGGTATCTCCATGATAACCATTGCGGAAGGTCAGAAAACGCTGGCGCGCTTCGCCTTTGGCTTGCCAGTACTGCaa       >  1:165316/1‑81 (MQ=255)                                                                       aGGTATCTCCATGATAACCATTGCGGAAGGTCAGAAAACGCTGGCGCGCTTCGCCTTTGGCTTGCCAGTACTGCAACGCCa  >  2:165326/1‑81 (MQ=255)                                                                              |                                                                          TTTTCTGGCAGGTAGCCTTTCCACAGACTGTGCATTGAGTTATCCGGATCGCACACCGACATCGCGCCAAAGGTATCGCCATGATAACCATTGCGGAAGGTCAGAAAACGCTGGCGCGCTTCGCCTTTGGCTTGCCAGTACTGCAACGCCA  >  NC_000913/808743‑808893

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑