Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	134,658	A→G	N91D (AAC→GAC)	yacL →	UPF0231 family protein YacL

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	134,658	0	A	G	100.0%	74.2 / NA	22	N91D (AAC→GAC)	yacL	UPF0231 family protein YacL
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (7/15);  total (7/15)

GGATGGACGGTGAAGAGGTGATGGTTCGCGCCAATCAACTGGAATTCGCTGGCGATGAAATGGAAGAGGGGATGAACTACTACGACGAAGAAAGCCTGTCGCTATGCGGCGTTGAGGATTTTCTGCAGGTCGTGGCGGCTTACCGCAATTT  >  NC_000913/134584‑134734                                                                           |                                                                             ggatggaCGGTGAAGAGGTGATGGTTCGCGCCAATCAACTGGAATTCGCTGGCGATGAAATGGAAGAGGGGATGGactac                                                                         >  1:30515/1‑80 (MQ=255)      gaCGGTGAAGAGGTGATGGTTCGCGCCAATCAACTGGAATTCGCTGGCGATGAAATGGAAGAGGGGATGGACTACTacgac                                                                   <  1:30463/81‑1 (MQ=255)      gaCGGTGAAGAGGTGATGGTTCGCGCCAATCAACTGGAATTCGCTGGCGATGAAATGGAAGAGGGGATGGACTACTacgac                                                                   <  2:30464/81‑1 (MQ=255)            gAAGAGGTGATGGTTCGCGCCAATCAACTGGAATTCGCTGGCGATGAAATGGAAGAGGGGATGGACTACTACGACgaaga                                                              >  2:30511/1‑80 (MQ=255)                     aTGGTTCGCGCCAATCAACTGGAATTCGCTGGCGATGAAATGGAAGAGGGGATGGACTACTACGACGAAGAAAGCCTGTc                                                     <  1:30466/80‑1 (MQ=255)                     aTGGTTCGCGCCAATCAACTGGAATTCGCTGGCGATGAAATGGAAGAGGGGATGGACTACTACGACGAAGAAAGCCTGTCg                                                    <  2:30465/81‑1 (MQ=255)                         ttCGCGCCAATCAACTGGAATTCGCTGGCGATGAAATGGAAGAGGGGATGGACTACTACGACGAAGAAAGCCTGTCGCTAt                                                <  2:30467/81‑1 (MQ=255)                            gcgcCAATCAACTGGAATTCGCTGGCGATGAAATGGAAGAGGGGATGGACTACTACGACGAAGAAAGCCTGTCGCTATgc                                              <  2:30468/80‑1 (MQ=255)                             cgcCAATCAACTGGAATTCGCTGGCGATGAAATGGAAGAGGGGATGGACTACTACGACGAAGAAAGCCTGTCGCTATgcgg                                            <  2:30469/81‑1 (MQ=255)                                     aaCTGGAATTCGCTGGCGATGAAATGGAAGAGGGGATGGACTACTACGACGAAGAAAGCCTGTCGCTATGCGGCGTTGAg                                     >  1:30525/1‑80 (MQ=255)                                            aTTCGCTGGCGATGAAATGGAAGAGGGGATGGACTACTACGACGAAGAAAGCCTGTCGCTATGCGGCGTTGAGGATTTTCt                             >  2:30520/1‑81 (MQ=255)                                                     cGATGAAATGGAAGAGGGGATGGACTACTACGACGAAGAAAGCCTGTCGCTATGCGGCGTTGAGGATTTTCTGCAGGTCGt                    >  2:30509/1‑81 (MQ=255)                                                      gATGAAATGGAAGAGGGGATGGACTACTACGACGAAGAAAGCCTGTCGCTATGCGGCGTTGAGGATTTTCTGCAGGTCGTg                   <  1:30470/81‑1 (MQ=255)                                                       aTGTAATGGAAGAGGGGATGGACTACTACGACGAAGAAAGCCTGTCGCTATGCGGCGTTGAGGATTTTCTGCAGGTCGTgg                  >  2:30514/1‑81 (MQ=255)                                                       aTGAAATGGAAGAGGGGATGGACTACTACGACGAAGAAAGCCTGTCGCTATGCGGCGTTGAGGATTTTCTGCAGGTCGTgg                  <  1:30471/81‑1 (MQ=255)                                                        tGAAATGGAAGAGGGGATGGACTACTACGACGAAGAAAGCCTGTCGCTATGCGGCGTTGAGGATTTTCTGCAGGTCGTggc                 <  2:30472/81‑1 (MQ=255)                                                          aaaTGGAAGAGGGGATGGACTACTACGACGAAGAAAGCCTGTCGCTATGCGGCGTTGAGGATTTTCTGCAGGTCGTggcg                <  1:30473/80‑1 (MQ=255)                                                            aTGGAAGAGGGGATGGACTACTACGACGAAGAAAGCCTGTCGCTATGCGGCGTTGAGGATTTTCTGCAGGTCGTGGCGGCt             >  2:30513/1‑81 (MQ=255)                                                               gAAGAGGGGATGGACTACTACGACGAAGAAAGCCTGTCGCTATGCGGCGTTGAGGATTTTCTGCAGGTCGTGGCGGCTTAc          <  1:30475/81‑1 (MQ=255)                                                               gAAGAGGGGATGGACTACTACGACGAAGAAAGCCTGTCGCTATGCGGCGTTGAGGATTTTCTGCAGGTCGTGGCGGCTTAc          <  1:30474/81‑1 (MQ=255)                                                                  gagGGGATGGACTACTACGACGAAGAAAGCCTGTCGCTATGCGGCGTTGAGGATTTTCTGCAGGTCGTGGCGGCTTACCGc       <  1:30476/81‑1 (MQ=255)                                                                       gATGGACTACTACGACGAAGAAAGCCTGTCGCTATGCGGCGTTGAGGATTTTCTGCAGGTCGTGGCGGCTTACCGCAAttt  <  2:30477/81‑1 (MQ=255)                                                                           |                                                                             GGATGGACGGTGAAGAGGTGATGGTTCGCGCCAATCAACTGGAATTCGCTGGCGATGAAATGGAAGAGGGGATGAACTACTACGACGAAGAAAGCCTGTCGCTATGCGGCGTTGAGGATTTTCTGCAGGTCGTGGCGGCTTACCGCAATTT  >  NC_000913/134584‑134734

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑