Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	862,428	A→T	L60Q (CTG→CAG)	ybiW ←	putative pyruvate formate lyase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	862,428	0	A	T	100.0%	153.0 / NA	42	L60Q (CTG→CAG)	ybiW	putative pyruvate formate lyase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (20/22);  total (20/22)

ATCGGCGCGGCGCGAACTTCGCTTGCCTGGTTGCCAATGATCAACTCATCGTGTTTGATCCAGATGGTGCGATTCGCCAGGTGATGCGCCAGTGCCAGCGCGCGACGTACCGGGATCGGCTTATCGAGATGTTGTTGATACATCTCGGTATAGTGCT  >  NC_000913/862350‑862506                                                                               |                                                                               aTCGGCGCGGCGCGAACTTCGCTTGCCTGGTTGCCAATGATCAACTCATCGTGTTTGATCCAGATGGTGCGATTCGCCTgg                                                                              >  2:175508/1‑81 (MQ=255)    ggcgcggcgcGAACTTCGCTTGCCTGGTTGCCAATGATCAACTCATCGTGTTTGATCCAGATGGTGCGATTCGCCTGGTg                                                                            <  1:175456/80‑1 (MQ=255)    ggcgcggcgcGAACTTCGCTTGCCTGGTTGCCAATGATCAACTCATCGTGTTTGATCCAGATGGTGCGATTCGCCTGGTGa                                                                           >  2:175488/1‑81 (MQ=255)     gcgcggcgcgAACTTCGCTTGCCTGGTTGCCAATGATCAACTCATCGTGTTTGATCCAGATGGTGCGATTCGCCTGGTGAt                                                                          <  2:175457/81‑1 (MQ=255)        cggcgcgAACTTCGCTTGCCTGGTTGCCAATGATCAACTCATCGTGTTTGATCCAGATGGTGCGATTCGCCTGGTGATgcg                                                                       >  1:175516/1‑81 (MQ=255)           cgcgAACTTCGCTTGCCTGGTTGCCAATGATCAACTCATCGTGTTTGATCCAGATGGTGCGATTCGCCTGGTGATgcgc                                                                      >  1:175524/1‑79 (MQ=255)            gcgAACTTCGCTTGCCTGGTTGCCAATGATCAACTCATCGTGTTTGATCCAGATGGTGCGATTCGCCTGGTGATGCGCCAg                                                                   >  1:175496/1‑81 (MQ=255)             cgAACTTCGCTTGCCTGGTTGCCAATGATCAACTCATCGTGTTTGATCCAGATGGTGCGATTCGCCTGGTGATGCGCCAGt                                                                  <  1:175458/81‑1 (MQ=255)                aCTTCGCTTGCCTGGTTGCCAATGATCAACTCATCGTGTTTGATCCAGATGGTGCGATTCGCCTGGTGATGCGCCAGTGcc                                                               <  1:175459/81‑1 (MQ=255)                   tCGCTTGCCTGGTTGCCAATGATCAACTCATCGTGTTTGATCCAGATGGTGCGATTCGCCTGGTGATGCGCCAGTGCCAgc                                                            >  1:175505/1‑81 (MQ=255)                   tCGCTTGCCTGGTTGCCAATGATCAACTCATCGTGTTTGATCCAGATGGTGCGATTCGCCTGGTGATGCGCCAGTGCCAgc                                                            >  1:175506/1‑81 (MQ=255)                    cGCTTGCCTGGTTGCCAATGATCAACTCATCGTGTTTGATCCAGATGGTGCGATTCGCCTGGTGATGCGCCAGTGCCAg                                                             <  1:175460/79‑1 (MQ=255)                    cGCTTGCCTGGTTGCCAATGATCAACTCATCGTGTTTGATCCAGATGGTGCGATTCGCCTGGTGATGCGCCAGTGCCAg                                                             <  2:175461/79‑1 (MQ=255)                      cTTGCCTGGTTGCCAATGATCAACTCATCGTGTTTGATCCAGATGGTGCGATTCGCCTGGTGATGCGCCAGTGCCAgcgcg                                                         >  2:175520/1‑81 (MQ=255)                        tGCCTGGTTGCCAATGATCAACTCATCGTGTTTGATCCAGATGGTGCGATTCGCCTGGTGATGCGCCAGTGCCAgcgcgc                                                        >  2:175509/1‑80 (MQ=255)                         gCCTGGTTGCCAATGATCAACTCATCGTGTTTGATCCAGATGGTGCGATTCGCCTGGTGATGCGCCAGTGCCAgcgcgc                                                        <  2:175462/79‑1 (MQ=255)                          ccTGGTTGCCAATGATCAACTCATCGTGTTTGATCCAGATGGTGCGATTCGCCTGGTGATGCGCCAGTGCCAGCGCGCGa                                                      <  2:175464/80‑1 (MQ=255)                          ccTGGTTGCCAATGATCAACTCATCGTGTTTGATCCAGATGGTGCGATTCGCCTGGTGATGCGCCAGTGCCAGCGCGCGAc                                                     <  1:175465/81‑1 (MQ=255)                          ccTGGTTGCCAATGATCAACTCATCGTGTTTGATCCAGATGGTGCGATTCGCCTGGTGATGCGCCAGTGCCAGCGCGCGAc                                                     <  2:175463/81‑1 (MQ=255)                                 gCCAATGATCAACTCATCGTGTTTGATCCAGATGGTGCGATTCGCCTGGTGATGCGCCAGTGCCAGCGCGCGACGTACCg                                               <  1:175466/80‑1 (MQ=255)                                    aaTGATCAACTCATCGTGTTTGATCCAGATGGTGCGATTCGAATGGTGATGCGCCAGTGCCAGCGCGCGACGTACCGGGAt                                           <  1:175467/81‑1 (MQ=255)                                     aTGATCAACTCATCGTGTTTGATCCAGATGGTGCGATTCGCCTGGTGATGCGCCAGTGCCAGCGCGCGACGTACCGGGATc                                          <  1:175468/81‑1 (MQ=255)                                     aTGATCAACTCATCGTGTTTGATCCAGATGGTGCGATTCGCCTGGTGATGCGCCAGTGCCAGCGCGCGACGTACCGGGATc                                          >  1:175517/1‑81 (MQ=255)                                        aTCAACTCATCGTGTTTGATCCAGATGGTGCGATTCGCCTGGTGATGCGCCAGTGCCAGCGCGCGACGTACCGGGATCGGc                                       >  1:175504/1‑81 (MQ=255)                                        aTCAACTCATCGTGTTTGATCCAGATGGTGCGATTCGCCTGGTGATGCGCCAGTGCCAGCGCGCGACGTACCGGGATCGGc                                       <  1:175469/81‑1 (MQ=255)                                          cAACTCATCGTGTTTGATCCACATGGTGCGATTCGCCTGGTGATGCGCCAGTGCCAGCGCGCGACGTACCGGGATCGGCt                                      >  2:175510/1‑80 (MQ=255)                                           aaCTCATCGTGTTTGATCCAGATGGTGCGATTCGCCTGGTGATGCGCCAGTGCCAGCGCGCGACGTACCGGGATCGGCtt                                     <  1:175470/80‑1 (MQ=255)                                            aCTCATCGTGTTTGATCCAGATGGTGCGATTCGCCTGGTGATGCGCCAGTGCCAGCGCGCGACGTACCGGGATCGGCTTAt                                   >  2:175515/1‑81 (MQ=255)                                            aCTCATCGTGTTTGATCCAGATGGTGCGATTCGCCTGGTGATGCGCCAGTGCCAGCGCGCGACGTACCGGGATCGGCTTAt                                   <  1:175471/81‑1 (MQ=255)                                             cTCATCGTGTTTGATCCAGATGGTGCGATTCACCTGGTGATGCGCCAGTGCCAGCGCGCGACGTACCGGGATCGGCTTATc                                  <  2:175472/81‑1 (MQ=255)                                                aTCGTGTTTGATCCAGATGGTGCGATTCGCCTGGTGATGCGCCAGTGCCAGCGCGCGACGTACCGGGATCGGCTTATc                                  >  1:175518/1‑78 (MQ=255)                                                 tCGTGTTTGATCCAGATGGTGCGATTCGCCTGGTGATGCGCCAGTGCCAGCGCGCGACGTACCGGGATCGGCTTATCgaga                              <  1:175473/81‑1 (MQ=255)                                                  cGTGTTTGATCCAGATGGTGCGATTCGCCTGGTGATGCGCCAGTGCCAGCGCGCGACGTACCGGGATCGGCTTATCGAGAt                             >  1:175522/1‑81 (MQ=255)                                                    tgtTTGATCCAGATGGTGCGATTCGCCTGGTGATGCGCCAGTGCCAGCGCGCGACGTACCGGGATCGGCTTATCGAGAtgt                           <  1:175474/81‑1 (MQ=255)                                                         gATCCAGATGGTGCGATTCGCCTGGTGATGCGCCAGTGCCAGCGCGCGACGTACCGGGATCGGCTTATCGAGATgttgtt                       >  1:175512/1‑80 (MQ=255)                                                               gATGGTGCGATTCGCCTGGTGATGCGCCAGTGCCAGCGCGCGACGTACCGGGATCGGCTTATCGAGATGTTGTTGATACAt                >  2:175530/1‑81 (MQ=255)                                                                aTGGTGCGATTCGCCTGGTGATGCGCCAGTGCCAGCGCGCGACGTACCGGGATCGGCTTATCGAGATGTTGTTGATACAtc               <  1:175475/81‑1 (MQ=255)                                                                  ggTGCGATTCGCCTGGTGATGCGCCAGTGCCAGCGCGCGACGTACCGGGATCGGCTTATCGAGATGTTGTTGATACAtctc             >  2:175513/1‑81 (MQ=255)                                                                   gTGCGATTCGCCTGGTGATGCGCCAGTGCCAGCGCGCGACGTACCGGGATCGGCTTATCGAGATGTTGTTGATACAtctc             <  1:175477/80‑1 (MQ=255)                                                                   gTGCGATTCGCCTGGTGATGCGCCAGTGCCAGCGCGCGACGTACCGGGATCGGCTTATCGAGATGTTGTTGATACAtctc             <  1:175476/80‑1 (MQ=255)                                                                   gTGCGATTCGCCTGGTGATGCGCCAGTGCCAGCGCGCGACGTACCGGGATCGGCTTATCGAGATGTTGTTGATACATCTCg            >  2:175525/1‑81 (MQ=255)                                                                            gCCTGGTGATGCGCCAGTGCCAGCGCGCGACGTACCGGGATCGGC‑TATCGAGATGTTGTTGATACATCTCGGTATAGtgct  >  2:175529/1‑81 (MQ=255)                                                                               |                                                                               ATCGGCGCGGCGCGAACTTCGCTTGCCTGGTTGCCAATGATCAACTCATCGTGTTTGATCCAGATGGTGCGATTCGCCAGGTGATGCGCCAGTGCCAGCGCGCGACGTACCGGGATCGGCTTATCGAGATGTTGTTGATACATCTCGGTATAGTGCT  >  NC_000913/862350‑862506

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑