Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	895,153	G→A	G55S (GGC→AGC)	potG →	putrescine ABC transporter ATP binding subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	895,153	0	G	A	100.0%	92.2 / NA	26	G55S (GGC→AGC)	potG	putrescine ABC transporter ATP binding subunit
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (14/12);  total (14/12)

CGATGGTCAACATGCGGTGGATGATGTCAGCCTGACCATCTACAAAGGTGAAATCTTCGCGCTGCTGGGCGCATCCGGCTGTGGCAAGTCCACGCTGCTGCGTATGCTGGCAGGTTTCGAACAACCTTCTGCCGGACAGATAATGCTTGATGG  >  NC_000913/895077‑895229                                                                             |                                                                             cGATGGTCAACATGCGGTGGATGATGTCAGCCTGACCATCTACAAAGGTGAAATCTTCGCGCTGCTGGGCGCATCCAGCt                                                                           <  2:181344/80‑1 (MQ=255)      gTCAACATGCGGTGGATGATGTCAGCCTGACCATCTACAAAGGTGAAATCTTCGCGCTGCTGGGCGCATCCAGCTGTgg                                                                       <  2:181345/79‑1 (MQ=255)             tGCGGTGGATGATGTCAGCCTGACCATCTACAAAGGTGAAATCTTCGCGCTGCTGGGCGCATCCAGCTGTGGCAAGTCCAc                                                              <  2:181346/81‑1 (MQ=255)                 gTGGATGATGTCAGCCTGACCATCTACAAAGGTGAAATCTTCGCGCTGCTGGGCGCATCCAGCTGTGGCAAGTCCACgctg                                                          >  1:181383/1‑81 (MQ=255)                         tgTCAGCCTGACCATCTACAAAGGTGAAATCTTCGCGCTGCTGGGCGCATCCAGCTGTGGCAAGTCCACGCTGCTGCGTAt                                                  <  2:181347/81‑1 (MQ=255)                         tgTCAGCCTGACCATCTACAAAGGTGAAATCTTCGCGCTGCTGGGCGCATCCAGCTGTGGCAAGTCCACGCTGCTGCGTAt                                                  >  1:181390/1‑81 (MQ=255)                          gTCAGCCTGACCATCTACAAAGGTGAAATCTTCGCGCTGCTGGGCGCATCCAGCTGTGGCAAGTCCACGCTGCTGCGTATg                                                 >  1:181382/1‑81 (MQ=255)                             aGCCTGACCATCTACAAAGGTGAAATCTTCGCGCTGCTGGGCGCATCCAGCTGTGGCAAGTCCACGCTGCTGCGTATGCTg                                              <  1:181348/81‑1 (MQ=255)                               ccTGACCATCTACAAAGGTGAAATCTTCGCGCTGCTGGGCGCATCCAGCTGTGGCAAGTCCACGCTGCTGCGTATGCTgg                                             >  2:181394/1‑80 (MQ=255)                                cTGACCATCTACAAAGGTGAAATCTTCGCGCTGCTGGGCGCATCCAGCTGTGGCAAGTCCACGCTGCTGCGTATGCTgg                                             >  2:181397/1‑79 (MQ=255)                                  gACCATCTACAAAGGTGAAATCTTCGCGCTGCTGGGCGCATCCAGCTGTGGCAAGTCCACGCTGCTGCGTATGCTGGCAgg                                         >  1:181400/1‑81 (MQ=255)                                   aCCATCTACAAAGGTGAAATCTTCGCGCTGCTGGGCGCATCCAGCTGTGGCAAGTCCACGCTGCTGCGTATGCTGGCAgg                                         <  1:181349/80‑1 (MQ=255)                                   aCCATCTACAAAGGTGAAATCTTCGCGCTGCTGGGCGCATCCAGCTGTGGCAAGTCCACGCTGCTGCGTATGCTGGCAGGt                                        >  2:181384/1‑81 (MQ=255)                                     cATCTACAAAGGTGAAATCTTCGCGCTGCTGGGCGCATCCAGCTGTGGCAAGTCCACGCTGCTGCGTATGCTGGCAGGttt                                      >  1:181402/1‑81 (MQ=255)                                      aTCTACAAAGGTGAAATCTTCGCGCTGCTGGGCGCATTCAGCTGTGGCAAGTCCACGCTGCTGCGTATGCTGGCAGGTTTc                                     >  2:181393/1‑81 (MQ=255)                                        cTACAAAGGTGAAATCTTCGCGCTGCTGGGCGCATCCAGCTGTGGCAAGTCCACGCTGCTGCGTATGCTGGCAGGTTTCg                                    >  1:181423/1‑80 (MQ=255)                                            aaaGGTGAAATCTTCGCGCTGCTGGGCGCATCCAGCTGTGGCAAGTCCACGCTGCTGCGTATGCTGGCAGGTTTCGaacaa                               >  2:181396/1‑81 (MQ=255)                                                         tCGCGCTGCTGGGCGCATCCAGCTGTGGCAAGTCCACGCTGCTGCGTATGCTGGCAGGTTTCGAACAACCTTCTGCCGGa                   >  1:181399/1‑80 (MQ=255)                                                           gcgcTGCTGGGCGCATCCAGCTGTGGCAAGTCCACGCTGCTGCGTATGCTGGCAGGTTTCGAACAACCTTCTGCCGGACAg                <  1:181350/81‑1 (MQ=255)                                                                gctgGGCGCATCCAGCTGTGGCAAGTCCACGCTGCTGCGTATGCTGGCAGGTTTCGAACAACCTTCTGCCGGACAGATAAt           >  2:181418/1‑81 (MQ=255)                                                                 ctgGGCGCATCCAGCTGTGGCAAGTCCACGCTGCTGCGTATGCTGGCAGGTTTCGAACAACCTTCTGCCGGACAGATAAt           <  1:181351/80‑1 (MQ=255)                                                                    ggCGCATCCAGCTGTGGCAAGTCCACGCTGCTGCGTATGCTGGCAGGTTTCGAACAACCTTCTGCCGGACAGATAATGCtt       >  1:181392/1‑81 (MQ=255)                                                                    ggCGCATCCAGCTGTGGCAAGTCCACGCTGCTGCGTATGCTGGCAGGTTTCGAACAACCTTCTGCCGGACAGATAATGCtt       <  1:181353/81‑1 (MQ=255)                                                                    ggCGCATCCAGCTGTGGCAAGTCCACGCTGCTGCGTATGCTGGCAGGTTTCGAACAACCTTCTGCCGGACAGATAATGCtt       <  1:181352/81‑1 (MQ=255)                                                                     gcgcATCCAGCTGTGGCAAGTCCACGCTGCTGCGTATGCTGGCAGGTTTCGAACAACCTTCTGCCGGACAGATAATGCTTg      <  2:181354/81‑1 (MQ=255)                                                                         aTCCAGCTGTGGCAAGTCCACGCTGCTGCGTATGCTGGCAGGTTTCGAACAACCTTCTGCCGGACAGATAATGCTTGATgg  <  1:181355/81‑1 (MQ=255)                                                                             |                                                                             CGATGGTCAACATGCGGTGGATGATGTCAGCCTGACCATCTACAAAGGTGAAATCTTCGCGCTGCTGGGCGCATCCGGCTGTGGCAAGTCCACGCTGCTGCGTATGCTGGCAGGTTTCGAACAACCTTCTGCCGGACAGATAATGCTTGATGG  >  NC_000913/895077‑895229

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑