Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	916,809	C→A	R113S (CGT→AGT)	ybjD →	DUF2813 domain‑containing protein YbjD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	916,809	0	C	A	100.0%	71.3 / NA	20	R113S (CGT→AGT)	ybjD	DUF2813 domain‑containing protein YbjD
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (9/11);  total (9/11)

TCGGGTTCGCCGTTATCGGCCGCTGGAAGCGTGCTGGACGCCATGCACCGATGGCTATCACCGTATTTTTTATCGTCTGGAAGGGGAGAGTGCGGAAGACGGCAGCGTGATGACACTGCGCAGTTTTCTCGATAAAGACGGACATC  >  NC_000913/916736‑916881                                                                          |                                                                         tCGGGTTCGCCGTTATCGGCCGCTGGAAGCGTGCTGGACGCCATGCACCGATGGCTATCACCGTATTTTTTATAGTCTGGa                                                                   <  1:185474/81‑1 (MQ=255)   gggTTCGCCGTTATCGGCCGCTGGAAGCGTGCTGGACGCCATGCACCGATGGCTATCACCGTATTTTTTATAGTCTGGAAg                                                                 <  1:185475/81‑1 (MQ=255)   gggTTCGCCGTTATCGGCCGCTGGAAGCGTGCTGGACGCCATGCACCGATGGCTATCACCGTATTTTTTATAGTCTGGAAg                                                                 >  2:185504/1‑81 (MQ=255)      ttCGCCGTTATCGGCCGCTGGAAGCGTGCTGGACGCCATGCACCGATGGCTATCACCGTATTTTTTATAGTCTGGAAgggg                                                              <  2:185476/81‑1 (MQ=255)             ttATCGGCCGCTGGAAGCGTGCTGGACGCCATGCACCGATGGCTATCACCGTATTTTTTATAGTCTGGAAGGGGAGAGTGc                                                       >  1:185507/1‑81 (MQ=255)              tATCGGCCGCTGGAAGCGTGCTGGACGCCATGCACCGATGGCTATCACCGTATTTTTTATAGTCTGGAAGGGGAGAGTGCg                                                      >  1:185498/1‑81 (MQ=255)                         ggAAGCGTGCTGGACGCCATGCACCGATGGCTATCACCGTATTTTTTATAGTCTGGAAGGGGAGAGTGCGGAAGACGGCAg                                           >  1:185501/1‑81 (MQ=255)                          gAAGCGTGCTGGACGCCATGCACCGATGGCTATCACCGTATTTTTTATAGTCTGGAAGGGGAGAGTGCGGAAGACGGCa                                            <  1:185477/79‑1 (MQ=255)                            aGCATGCTGGACGCCATGCACCGATGGCTATCACCGTATTTTTTATAGTCTGGAAGGGGAGAGTGCGGAAGACGGCAGCGt                                        <  1:185478/81‑1 (MQ=255)                               gTGCTGGACGCCATGCACCGATGGCTATCACCGTATTTTTTATAGTCTGGAAGGGGAGAGTGCGGAAGACGGCAGCGtgat                                     <  1:185480/81‑1 (MQ=255)                               gTGCTGGACGCCATGCACCGATGGCTATCACCGTATTTTTTATAGTCTGGAAGGGGAGAGTGCGGAAGACGGCAGCGtgat                                     <  1:185479/81‑1 (MQ=255)                                     gACGCCATGCACCGATGGCTATCACCGTATTTTTTATAGTCTGGAAGGGGAGAGTGCGGAAGACGGCAGCGTGATGACACt                               >  2:185510/1‑81 (MQ=255)                                     gACGCCATGCACCGATGGCTATCACCGTATTTTTTATAGTCTGGAAGGGGAGAGTGCGGAAGACGGCAGCGTGATGACACt                               >  2:185514/1‑81 (MQ=255)                                              cACCGATGGCTATCACCGTATTTTTTATAGTCTGGAAGGGGAGAGTGCGGAAGACGGCAGCGTGATGACACTGCGCAGtt                       <  1:185481/80‑1 (MQ=255)                                               aCCGATGGCTATCACCGTATTTTTTATAGTCTGGAAGGGGAGAGTGCGGAAGACGGCAGCGTGATGACACTGCGCAGtttt                     >  2:185506/1‑81 (MQ=255)                                                ccGATGGCTATCACCGTATTTTTTATAGTCTGGAAGGGGAGAGTGCGGAAGACGGCAGCGTGATGACACTGCGCAGTTTtc                    <  1:185482/81‑1 (MQ=255)                                                   aTGGCTATCACCGTATTTTTTATAGTCTGGAAGGGGAGAGTGCGGAAGACGGCAGCGTGATGACACTGCGCAGTTTTCTCg                 <  1:185483/81‑1 (MQ=255)                                                     ggCTATCACCGTATTTTTTATAGTCTGGAAGGGGAGAGTGCGGAAGACGGCAGCGTGATGACACTGCGCAGTTTTCTCGa                >  2:185508/1‑80 (MQ=255)                                                              cGTATTTTTTATAGTCTGGAAGGGGAGAGTGCGGAAGACGGCAGCGTGATGACACTGCGCAGTTTTCTCGATAAAGACGGa      >  2:185511/1‑81 (MQ=255)                                                                  ttttttATAGTCTGGAAGGGGAGAGTGCGGAAGACGGCAGCGTGATGACACTGCGCAGTTTTCTCGATAAAGACGGACATc  <  1:185484/81‑1 (MQ=255)                                                                          |                                                                         TCGGGTTCGCCGTTATCGGCCGCTGGAAGCGTGCTGGACGCCATGCACCGATGGCTATCACCGTATTTTTTATCGTCTGGAAGGGGAGAGTGCGGAAGACGGCAGCGTGATGACACTGCGCAGTTTTCTCGATAAAGACGGACATC  >  NC_000913/916736‑916881

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑