Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	974,632	C→A	P105Q (CCG→CAG)	mukF →	chromosome partitioning protein MukF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	974,632	0	C	A	100.0%	187.3 / NA	50	P105Q (CCG→CAG)	mukF	chromosome partitioning protein MukF
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (19/31);  total (19/31)

ACGACATGGTGCGTCAACGTCTGCTGAACCGCTTTACCAGCGAGCAGGCGGAAGGGAACGCAATTTACCGTCTGACGCCGCTCGGCATCGGCATTACTGACTACTACATCCGTCAGCGCGAGTTTTCTACGCTGCGTCTTTCTATGCAGTTGTCGA  >  NC_000913/974554‑974709                                                                               |                                                                              aCGACATGGTGCGTCAACGTCTGCTGAACCGCTTTACCAGCGAGCAGGCGGAAGGGAACGCAATTTACCGTCTGAcgcagc                                                                             <  2:195863/81‑1 (MQ=255)   gACATGGTGCGTCAACGTCTGCTGAACCGCTTTACCAGCGAGCAGGCGGAAGGGAACGCAATTTACCGTCTGACGCAGCtc                                                                           <  1:195865/81‑1 (MQ=255)   gACATGGTGCGTCAACGTCTGCTGAACCGCTTTACCAGCGAGCAGGCGGAAGGGAACGCAATTTACCGTCTGACGCAGCtc                                                                           <  1:195864/81‑1 (MQ=255)    aCATGGTGCGTCAACGTCTGCTGAACCGCTTTACCAGCGAGCAGGCGGAAGGGAACGCAATTTACCGTCTGACGCAGCtcg                                                                          <  2:195866/81‑1 (MQ=255)     cATGGTGCGTCAACGTCTGCTGAACCGCTTTACCAGCGAGCAGGCGGAAGGGAACGCAATTTACCGTCTGACGCAGCtcgg                                                                         <  2:195867/81‑1 (MQ=255)          tGCGTCAACGTCTGCTGAACCGCTTTACCAGCGAGCAGGCGGAAGGGAACGCAATTTACCGTCTGACGCAGCtcggcatcg                                                                    >  2:195919/1‑81 (MQ=255)           gCGTCAACGTCTGCTGAACCGCTTTACCAGCGAGCAGGCGGAAGGGAACGCAATTTACCGTCTGACGCAGCtcggcatcgg                                                                   >  1:195946/1‑81 (MQ=255)             gTCAACGTCTGCTGAACCGCTTTACCAGCGAGCAGGCGGAAGGGAACGCAATTTACCGTCTGACGCAGCtcggcatcggca                                                                 <  2:774472/81‑1 (MQ=255)              tCAACGTCTGCTGAACCGCTTTACCAGCGAGCAGGCGGAAGGGAACGCAATTTACCGTCTGACGCAGCTcggcatcggcat                                                                <  1:195868/81‑1 (MQ=255)               cAACGTCTGCTGAACCGCTTTACCAGCGAGCAGGCGGAAGGGAACGCAATTTACCGTCTGACGCAGCTcggcatcggcat                                                                >  2:195924/1‑80 (MQ=255)                aaCGTCTGCTGAACCGCTTTACCAGCGAGCAGGCGGAAGGGAACGCAATTTACCGTCTGACGCAGCTCGGCATCGGCAtt                                                               <  1:195870/80‑1 (MQ=255)                aaCGTCTGCTGAACCGCTTTACCAGCGAGCAGGCGGAAGGGAACGCAATTTACCGTCTGACGCAGCTCGGCATCGGCAtt                                                               <  1:195869/80‑1 (MQ=255)                aaCGTCTGCTGAACCGCTTTACCAGCGAGCAGGCGGAAGGGAACGCAATTTACCGTCTGACGCAGCTCGGCATCGGCAtt                                                               <  1:195871/80‑1 (MQ=255)                aaCGTCTGCTGAACCGCTTTACCAGCGAGCAGGCGGAAGGGAACGCAATTTACCGTCTGACGCAGCTCGGCATCGGCATTa                                                              >  2:195944/1‑81 (MQ=255)                 aCGTCTGCTGAACCGCTTTACCAGCGAGCAGGCGGAAGGGAACGCAATTTACCGTCTGACGCAGCTCGGCATCGGCATTAc                                                             >  1:195920/1‑81 (MQ=255)                     ctgctgAACCGCTTTACCAGCGAGCAGGCGGAAGGGAACGCAATTTACCGTCTGACGCAGCTCGGCATCGGCATTACTGac                                                         >  2:195929/1‑81 (MQ=255)                      tgctgAACCGCTTTACCAGCGAGCAGGCGGAAGGGAACGCAATTTACCGTCTGACGCAGCTCGGCATCGGCATTACTGac                                                         >  2:195945/1‑80 (MQ=255)                        ctgAACCGCTTTACCAGCGAGCAGGCGGAAGGGAACGCAATTTACCGTCTGACGCAGCTCGGCATCGGCATTACTGac                                                         <  2:195872/78‑1 (MQ=255)                               gCTTTACCAGCGAGCAGGCGGAAGGGAACGCAATTTACCGTCTGACGCAGCTCGGCATCGGCATTACTGACTACTACATcc                                               <  2:195873/81‑1 (MQ=255)                               gCTTTACCAGCGAGCAGGCGGAAGGGAACGCAATTTACCGTCTGACGCAGCTCGGCATCGGCATTACTGACTACTACATcc                                               <  1:195874/81‑1 (MQ=255)                                cTTTACCAGCGAGCAGGCGGAAGGGAACGCAATTTACCGTCTGACGCAGCTCGGCATCGGCATTACTGACTACTACATCCg                                              >  1:195934/1‑81 (MQ=255)                                cTTTACCAGCGAGCAGGCGGAAGGGAACGCAATTTACCGTCTGACGCAGCTCGGCATCGGCATTACTGACTACTACATCCg                                              <  1:195875/81‑1 (MQ=255)                                  ttACCAGCGAGCAGGCGGAAGGGAACGCAATTTACCGTCTGACGCAGCTCGGCATCGGCATTACTGACTACTACATCCGt                                             >  1:195939/1‑80 (MQ=255)                                   tACCAGCGAGCAGGCGGAAGGGAACGCAATTTACCGTCTGACGCAGCTCGGCATCGGCATTACTGACTACTACATCCGt                                             <  2:195876/79‑1 (MQ=255)                                   tACCAGCGAGCAGGCGGAAGGGAACGCAATTTACCGTCTGACGCAGCTCGGCATCGGCATTACTGACTACTACATCCGTc                                            <  2:195877/80‑1 (MQ=255)                                    aCCAGCGAGCAGGCGGAAGGGAACGCAATTTACCGTCTGACGCAGCTCGGCATCGGCATTACTGACTACTACATCCGTCAg                                          >  2:195938/1‑81 (MQ=255)                                     ccAGCGAGCAGGCGGAAGGGAACGCAATTTACCGTCTGACGCAGCTCGGCATCGGCATTACTGACTACTACATCCGTCAgc                                         <  1:195878/81‑1 (MQ=255)                                      cAGCGAGCAGGCGGAAGGGAACGCAATTTACCGTCTGACGCAGCTCGGCATCGGCATTACTGACTACTACATCCGTCAgcg                                        <  1:195879/81‑1 (MQ=255)                                       aGCGAGCAGGCGGAAGGGAACGCAATTTACCGTCTGACGCAGCTCGGCATCGGCATTACTGACTACTACATCCGTCAgcgc                                       >  2:195951/1‑81 (MQ=255)                                           aGCAGGCGGAAGGGAACGCAATTTACCGTCTGACGCAGCTCGGCATCGGCATTACTGACTACTACATCCGTCAGCGCGAGt                                   <  2:195880/81‑1 (MQ=255)                                              aGGCGGAAGGGAACGCAATTTACCGTCTGACGCAGCTCGGCATCGGCATTACTGACTACTACATCCGTCAGCGCGAGtttt                                >  1:195933/1‑81 (MQ=255)                                               ggCGGAAGGGAACGCAATTTACCGTCTGACGCAGCTCGGCATCGGCATTACTGACTACTACATCCGTCAGCGCGAGTTTTc                               <  1:195881/81‑1 (MQ=255)                                                gCGGAAGGGAACGCAATTTACCGTCTGACGCAGCTCGGCATCGGCATTACTGACTACTACATCCGTCAGCGCGAGTTTTc                               >  2:195931/1‑80 (MQ=255)                                                 cGGAAGGGAACGCAATTTACCGTCTGACGCAGCTCGGCATCGGCATTACTGACTACTACATCCGTCAGCGCGAGTTTTCTa                             <  2:195882/81‑1 (MQ=255)                                                    aaGGGAACGCAATTTACCGTCTGACGCAGCTCGGCATCGGCATTACTGACTACTACATCCGTCAGCGCGAGTTTTCTACGc                          >  1:195932/1‑81 (MQ=255)                                                    aaGGGAACGCAATTTACCGTCTGACGCAGCTCGGCATCGGCATTACTGACTACTACATCCGTCAGCGCGAGTTTTCTACGc                          >  2:195947/1‑81 (MQ=255)                                                    aaGGGAACGCAATTTACCGTCTGACGCAGCTCGGCATCGGCATTACTGACTACTACATCCGTCAGCGCGAGTTTTCTACGc                          <  1:195883/81‑1 (MQ=255)                                                          aCGCAATTTACCGTCTGACGCAGCTCGGCATCGGCATTACTGACTACTACATCCGTCAGCGCGAGTTTTCTACGCTGCGTc                    <  1:195884/81‑1 (MQ=255)                                                             cAATTTACCGTCTGACGCAGCTCGGCATCGGCATTACTGACTACTACATCCGTCAGCGCGAGTTTTCTACGCTGCGTCttt                 <  2:195885/81‑1 (MQ=255)                                                             cAATTTACCGTCTGACGCAGCTCGGCATCGGCATTACTGACTACTACATCCGTCAGCGCGAGTTTTCTACGCTGCGTCttt                 >  2:195948/1‑81 (MQ=255)                                                              aaTTTACCGTCTGACGCAGCTCGGCATCGGCATTACTGACTACTACATCCGTCAGCGCGAGTTTTCTACGCTGCGTCTTTc                <  2:195887/81‑1 (MQ=255)                                                              aaTTTACCGTCTGACGCAGCTCGGCATCGGCATTACTGACTACTACATCCGTCAGCGCGAGTTTTCTACGCTGCGTCTTTc                <  1:195886/81‑1 (MQ=255)                                                                  tACCGTCTGACGCAGCTCGGCATCGGCATTACTGACTACTACATCCGTCAGCGCGAGTTTTCTACGCTGCGTCTTTCTATg            >  2:195942/1‑81 (MQ=255)                                                                    ccGTCTGACGCAGCTCGGCATCGGCATTACTGACTACTACATCCGTCAGCGCGAGTTTTCTACGCTGCGTCTTTCTATGc           <  1:195888/80‑1 (MQ=255)                                                                     cGTCTGACGCAGCTCGGCATCGGCATTACTGACTACTACATCCGTCAGCGCGAGTTTTCTACGCTGCGTCTTTCTATGCAg         <  1:195889/81‑1 (MQ=255)                                                                       tCTGACGCAGCTCGGCATCGGCATTACTGACTACTACATCCGTCAGCGCGAGTTTTCTACGCTGCGTCTTTCTATGCAGtt       <  1:195890/81‑1 (MQ=255)                                                                        cTGACGCAGCTCGGCATCGGCATTACTGACTACTACATCCGTCAGCGCGAGTTTTCTACGCTGCGTCTTTCTATGCAGTTg      >  2:316317/1‑81 (MQ=255)                                                                         tGACGCAGCTCGGCATCGGCATTACTGACTACTACATCCGTCAGCGCGAGTTTTCTACGCTGCGTCTTTCTATGCAGTTGt     >  1:195937/1‑81 (MQ=255)                                                                           aCGCAGCTCGGCATCGGCATTACTGACTACTACATCCGTCAGCGCGAGTTTTCTACGCTGCGTCTTTCTATGCAGTTGt     <  2:195893/79‑1 (MQ=255)                                                                           aCGCAGCTCGGCATCGGCATTACTGACTACTACATCCGTCAGCGCGAGTTTTCTACGCTGCGTCTTTCTATGCAGTTGt     <  1:195891/79‑1 (MQ=255)                                                                           aCGCAGCTCGGCATCGGCATTACTGACTACTACATCCGTCAGCGCGAGTTTTCTACGCTGCGTCTTTCTATGCAGTTGTCg   <  1:195892/81‑1 (MQ=255)                                                                            cgcagcTCGGCATCGGCATTACTGACTACTACATCCGTCAGCGCGAGTTTTCTACGCTGCGTCTTTCTATGCAGTTGTCGa  <  1:195894/81‑1 (MQ=255)                                                                            cgcagcTCGGCATCGGCATTACTGACTACTACATCCGTCAGCGCGAGTTTTCTACGCTGCGTCTTTCTATGCAGTTGTCGa  <  1:195895/81‑1 (MQ=255)                                                                               |                                                                              ACGACATGGTGCGTCAACGTCTGCTGAACCGCTTTACCAGCGAGCAGGCGGAAGGGAACGCAATTTACCGTCTGACGCCGCTCGGCATCGGCATTACTGACTACTACATCCGTCAGCGCGAGTTTTCTACGCTGCGTCTTTCTATGCAGTTGTCGA  >  NC_000913/974554‑974709

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑