Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	982,951	C→T	intergenic (+57/‑124)	ldtD → / → ycbK	L,D‑transpeptidase LdtD/DUF882 domain‑containing protein YcbK

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	982,951	0	C	T	100.0%	82.8 / NA	23	intergenic (+57/‑124)	ldtD/ycbK	L,D‑transpeptidase LdtD/DUF882 domain‑containing protein YcbK
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (12/11);  total (12/11)

AGCGGAACAATTAATCAGGTAAATGAAGTAGTTCGGGGAAATCAGTTGTCGTAATAATTAGCATGATTGGGGGCGATTCTCTGCAGCCCCCGTCACTGCTGGGGTTGAGTCATCTTGACGTCTGCTTTACGGGCGGTTAAGGTGCCTCTTGTGCG  >  NC_000913/982873‑983027                                                                               |                                                                             aGCGGAACAATTAATCAGGTAAATGAAGTAGTTCGGGGAAATCAGTTGTCGTAATAATTAGCATGATTGGGGGCGATtttc                                                                            >  2:197727/1‑81 (MQ=255) aGCGGAACAATTAATCAGGTAAATGAAGTAGTTCGGGGAAATCAGTTGTCGTAATAATTAGCATGATTGGGGGCGATtttc                                                                            >  2:197724/1‑81 (MQ=255) aGCGGAACAATTAATCAGGTAAATGAAGTAGTTCGGGGAAATCAGTTGTCGTAATAATTAGCATGATTGGGGGCGATtttc                                                                            >  1:197726/1‑81 (MQ=255)      aaCAATTAATCAGGTAAATGAAGTAGTTCGGGGAAATCAGTTGTCGTAATAATTAGCATGATTGGGGGCGATTTTCTGCAg                                                                       >  1:197735/1‑81 (MQ=255)              aTCAGGTAAATGAAGTAGTTCGGGGAAATCAGTTGTCGTAATAATTAGCATGATTGGGGGCGATTTTCTGCAGCCCCCGt                                                                <  1:197693/80‑1 (MQ=255)                cAGGTAAATGAAGTAGTTCGGGGAAATCAGTTGTCGTAATAATTAGCATGATTGGGGGCGATTTTCTGCAGCCCCCGTCAc                                                             >  1:197732/1‑81 (MQ=255)                      aaTGAAGTAGTTCGGGGAAATCAGTTGTCGTAATAATTAGCATGATTGGGGGCGATTTTCTGCAGCCCCCGTCACTGCTgg                                                       >  1:197728/1‑81 (MQ=255)                         gAAGTAGTTCGGGGAAATCAGTTGTCGTAATAATTAGCATGATTGGGGGCGATTTTCTGCAGCCCCCGTCACTGCTGGGGt                                                    >  2:197729/1‑81 (MQ=255)                           agtagtTCGGGGAAATCAGTTGTCGTAATAATTAGCATGATTGGGGGCGATTTTCTGCAGCCCCCGTCACTGCTGGGGTTg                                                  <  1:197694/81‑1 (MQ=255)                               gtTCGGGGAAATCAGTTGTCGTAATAATTAGCATGATTGGGGGCGATTTTCTGCAGCCCCCGTCACTGCTGGGGTTGAGTc                                              <  1:197695/81‑1 (MQ=255)                                     ggAAATCAGTTGTCGTAATAATTAGCATGATTGGGGGCGATTTTCTGCAGCCCCCGTCACTGCTGGGGTTGAGTTATCtt                                         >  2:197737/1‑80 (MQ=255)                                     ggAAATCAGTTGTCGTAATAATTAGCATGATTGGGGGCGATTTTCTGCAGCCCCCGTCACTGCTGGGGTTGAGTCATCtt                                         >  1:197745/1‑80 (MQ=255)                                                   gTAATAATTAGCATGATTGGGGGCGATTTTCTGCAGCCCCCGTCACTGCTGGGGTTGAGTCATCTTGACGTCTGCTTTACg                          >  1:197736/1‑81 (MQ=255)                                                      ataatTAGCATGATTGGGGGCGATTTTCTGCAGCCCCCGTCACTGCTGGGGTTGAGTCATCTTGACGTCTGCTTTACGGGc                       <  1:197696/81‑1 (MQ=255)                                                            aGCATGATTGGGGGCGATTTTCTGCAGCCCCCGTCACTGCTGGGGTTGAGTCATCTTGACGTCTGCTTTACGGGCGGTTa                  <  1:197697/80‑1 (MQ=255)                                                                 gATTGGGGGCGATTTTCTGCAGCCCCCGTCACTGCTGGGGTTGAGTCATCTTGACGTCTGCTTTACGGGCGGTTAAGGTGc            >  2:197753/1‑81 (MQ=255)                                                                     gggggCGATTTTCTGCAGCCCCCGTCACTGCTGGGGTTGAGTCATCTTGACGTCTGCTTTACGGGCGGTTAAGGTGCctct        >  1:197733/1‑81 (MQ=255)                                                                     gggggCGATTTTCTGCAGCCCCCGTCACTGCTGGGGTTGAGTCATCTTGACGTCTGCTTTACGGGCGGTTAAGGTGCctct        >  2:197742/1‑81 (MQ=255)                                                                      ggggCGATTTTCTGCAGCCCCCGTCACTGCTGGGGTTGAGTCATCTTGACGTCTGCTTTACGGGCGGTTAAGGTGCctct        <  2:197701/80‑1 (MQ=255)                                                                      ggggCGATTTTCTGCAGCCCCCGTCACTGCTGGGGTTGAGTCATCTTGACGTCTGCTTTACGGGCGGTTAAGGTGCCTCtt       <  1:197700/81‑1 (MQ=255)                                                                      ggggCGATTTTCTGCAGCCCCCGTCACTGCTGGGGTTGAGTCATCTTGACGTCTGCTTTACGGGCGGTTAAGGTGCCTCtt       <  1:197699/81‑1 (MQ=255)                                                                      ggggCGATTTTCTGCAGCCCCCGTCACTGCTGGGGTTGAGTCATCTTGACGTCTGCTTTACGGGCGGTTAAGGTGCCTCtt       <  1:197698/81‑1 (MQ=255)                                                                       gggCGATTTTCTGCAGCCCCCGTCACTGCTGGGGTTGAGTCATCTTGACGTCTGCTTTACGGGCGGTTAAGGTGCCTCTtg      <  2:197702/81‑1 (MQ=255)                                                                          cGATTTTCTGCAGCCCCCGTCACTGCTGGGGTTGAGTCATCTTGACGTCTGCTTTACGGGCGGTTAAGGTGCCTCTTGTgc   <  2:197703/81‑1 (MQ=255)                                                                           gATTTTCTGCAGCCCCCGTCACTGCTGGGGTTGAGTCATCTTGACGTCTGCTTTACGGGCGGTTAAGGTGCCTCTTGTgcg  >  1:197738/1‑81 (MQ=255)                                                                           gATTTTCTGCAGCCCCCGTCACTGCTGGGGTTGAGTCATCTTGACGTCTGCTTTACGGGCGGTTAAGGTGCCTCTTGTgcg  >  1:197747/1‑81 (MQ=255)                                                                               |                                                                             AGCGGAACAATTAATCAGGTAAATGAAGTAGTTCGGGGAAATCAGTTGTCGTAATAATTAGCATGATTGGGGGCGATTCTCTGCAGCCCCCGTCACTGCTGGGGTTGAGTCATCTTGACGTCTGCTTTACGGGCGGTTAAGGTGCCTCTTGTGCG  >  NC_000913/982873‑983027

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑